Restriction map for
pFN21AE1451
(Circular) (Six-base) MAPSORT of: pFN21AE1451.seq Check: 9999 from: 1 to: 5782
pFN21AE1451 5782 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2432 2983 2432
Size: 551 5231
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3592 278
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3125 1937
Size: 1188 4594
Acc65I G'GTAC_C
Cuts at: 3103 3103
Size: 5782
AclI AA'CG_TT
Cuts at: 1563 3909 4282 1563
Size: 2346 373 3063
Fragments arranged by size:
3063 2346 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2759 3845 5229 5497 1632
Size: 1127 1086 1384 268 1917
Fragments arranged by size:
1917 1384 1127 1086 268
AfeI AGC'GCT
Cuts at: 3002 3002
Size: 5782
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5585
AflIII A'CryG_T
Cuts at: 1203 4681 5658 1203
Size: 3478 977 1327
Fragments arranged by size:
3478 1327 977
AgeI A'CCGG_T
Cuts at: 4613 4613
Size: 5782
AhdI GACnn_n'nnGTC
Cuts at: 4511 4511
Size: 5782
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5782
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5782
AlwNI CAG_nnn'CTG
Cuts at: 2062 3525 5097 2062
Size: 1463 1572 2747
Fragments arranged by size:
2747 1572 1463
ApaLI G'TGCA_C
Cuts at: 3049 3839 4995 3049
Size: 790 1156 3836
Fragments arranged by size:
3836 1156 790
ApoI r'AATT_y
Cuts at: 1493 3091 3317 3469 5578 1493
Size: 1598 226 152 2109 1697
Fragments arranged by size:
2109 1697 1598 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4336 160
Size: 872 3304 1606
Fragments arranged by size:
3304 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
AvaI C'yCGr_G
Cuts at: 1870 1943 2114 2159 2463 2538 2671 3107 1870
Size: 73 171 45 304 75 133 436 4545
Fragments arranged by size:
4545 436 304 171 133 75 73 45
BamHI G'GATC_C
Cuts at: 3112 3479 3112
Size: 367 5415
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2132 3103 4558 618
Size: 325 217 627 345 971 1455 1842
Fragments arranged by size:
1842 1455 971 627 345 325 217
BanII G_rGCy'C
Cuts at: 1391 2056 2166 2418 2891 2952 3101 1391
Size: 665 110 252 473 61 149 4072
Fragments arranged by size:
4072 665 473 252 149 110 61
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4811
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3040 3074 3973 4007 1903
Size: 34 50 34 1019 34 899 34 3678
Fragments arranged by size:
3678 1019 899 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3674 4884 3674
Size: 1210 4572
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5782
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2409 4393 136
Size: 107 122 71 631 605 737 1984 1525
Fragments arranged by size:
1984 1525 737 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5777 5777
Size: 5782
BlpI GC'TnA_GC
Cuts at: 2055 3199 2055
Size: 1144 4638
Bme1580I G_kGCm'C
Cuts at: 1790 2137 2900 3053 3843 4999 1790
Size: 347 763 153 790 1156 2573
Fragments arranged by size:
2573 1156 790 763 347 153
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5782
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3513 4471 442
Size: 416 53 83 399 24 2096 958 1753
Fragments arranged by size:
2096 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5782
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2813 4442 1239
Size: 1574 1629 2579
Fragments arranged by size:
2579 1629 1574
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2672 3907 4772 5070 2672
Size: 1235 865 298 3384
Fragments arranged by size:
3384 1235 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2104 2336 4445 882
Size: 617 605 232 2109 2219
Fragments arranged by size:
2219 2109 617 605 232
BsaAI yAC'GTr
Cuts at: 493 1204 3573 493
Size: 711 2369 2702
Fragments arranged by size:
2702 2369 711
BsaBI GATnn'nnATC
Cuts at: 2123 5776 2123
Size: 3653 2129
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2070 2667 3589 3971
Size: 53 83 186 936 537 597 922 382
Cuts at: 3971 4606 275
Size: 635 1451
Fragments arranged by size:
1451 936 922 635 597 537 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4215 4613 4887 5034 5445 4215
Size: 398 274 147 411 4552
Fragments arranged by size:
4552 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2060 2090 1178
Size: 30 165 30 657 30 4870
Fragments arranged by size:
4870 657 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2588 1186
Size: 1402 4380
BseYI C'CCAG_C
Cuts at: 2009 2049 2136 2817 2852 4985 5481 2009
Size: 40 87 681 35 2133 496 2310
Fragments arranged by size:
2310 2133 681 496 87 40 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2126 2167 3011 1163
Size: 573 390 41 844 3934
Fragments arranged by size:
3934 844 573 390 41
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2620 2763 3232 3993 5021 1540
Size: 275 186 619 143 469 761 1028 2301
Fragments arranged by size:
2301 1028 761 619 469 275 186 143
BsiHKAI G_wGCw'C
Cuts at: 2208 2418 2592 2885 2952 3053 3843 3928 4999
Size: 210 174 293 67 101 790 85 1071
Cuts at: 4999 2208
Size: 2991
Fragments arranged by size:
2991 1071 790 293 210 174 101 85
67
BsmI GAATG_Cn'
Cuts at: 2255 3293 3386 2255
Size: 1038 93 4651
Fragments arranged by size:
4651 1038 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2674 2674
Size: 5782
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2056 2137 2166 2208 2418 2592 2885
Size: 399 266 81 29 42 210 174 293
Cuts at: 2885 2891 2900 2952 3053 3101 3843 3928 4999
Size: 6 9 52 101 48 742 85 1071
Cuts at: 4999 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 293 266 210 174
101 85 81 52 48 42 29 9
6
BspEI T'CCGG_A
Cuts at: 5445 5445
Size: 5782
BspHI T'CATG_A
Cuts at: 2592 3671 5401 2592
Size: 1079 1730 2973
Fragments arranged by size:
2973 1730 1079
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2432 2466 2607 2702 2983 3137 844
Size: 1588 34 141 95 281 154 3489
Fragments arranged by size:
3489 1588 281 154 141 95 34
BsrBI CCG'CTC
Cuts at: 1394 1823 3669 1394
Size: 429 1846 3507
Fragments arranged by size:
3507 1846 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4277 4451 65
Size: 1183 3029 174 1396
Fragments arranged by size:
3029 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2615 2733 2787 4426 4613 1700
Size: 110 805 118 54 1639 187 2869
Fragments arranged by size:
2869 1639 805 187 118 110 54
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5782
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5782
BssSI C'ACGA_G
Cuts at: 3842 4854 3842
Size: 1012 4770
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5782
BstBI TT'CG_AA
Cuts at: 4586 4586
Size: 5782
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5782
BstYI r'GATC_y
Cuts at: 1972 3112 3479 3864 3881 5333 5419 5431 5777
Size: 1140 367 385 17 1452 86 12 346
Cuts at: 5777 1972
Size: 1977
Fragments arranged by size:
1977 1452 1140 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2370 2662 513
Size: 552 1305 292 3633
Fragments arranged by size:
3633 1305 552 292
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5043
Fragments arranged by size:
5043 683 56
BtsI GCAGTG_nn'
Cuts at: 3007 3298 4092 4112 3007
Size: 291 794 20 4677
Fragments arranged by size:
4677 794 291 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5782
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5747
DraI TTT'AAA
Cuts at: 3086 3086
Size: 5782
DraIII CAC_nnn'GTG
Cuts at: 2362 2362
Size: 5782
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4789 809
Size: 507 3473 1802
Fragments arranged by size:
3473 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2173 2709 2760
Size: 54 1425 50 275 18 343 536 51
Cuts at: 2760 3229 4118 5747 8
Size: 469 889 1629 43
Fragments arranged by size:
1629 1425 889 536 469 343 275 54
51 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2760 3229 1537
Size: 275 948 469 4090
Fragments arranged by size:
4090 948 469 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3712 1353
Size: 2359 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4369 4743 4889 387
Size: 1064 138 2780 374 146 1280
Fragments arranged by size:
2780 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2416 2950 2416
Size: 534 5248
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2759 2813 3845 4442 5229 5497 1239
Size: 393 1127 54 1032 597 787 268 1524
Fragments arranged by size:
1524 1127 1032 787 597 393 268 54
EcoO109I rG'GnC_Cy
Cuts at: 2455 2676 2833 2455
Size: 221 157 5404
Fragments arranged by size:
5404 221 157
EcoRI G'AATT_C
Cuts at: 3091 3469 3091
Size: 378 5404
FspI TGC'GCA
Cuts at: 2873 4288 2873
Size: 1415 4367
HaeII r_GCGC'y
Cuts at: 1678 2370 3004 4929 1678
Size: 692 634 1925 2531
Fragments arranged by size:
2531 1925 692 634
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2060 2092 2288 2320 4423
Size: 32 135 32 488 32 196 32 2103
Cuts at: 4423 4455 4497 4529 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2103 488 196 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2383 2803 3126 3372 669
Size: 988 281 445 420 323 246 3079
Fragments arranged by size:
3079 988 445 420 323 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5782
HpaI GTT'AAC
Cuts at: 3372 3372
Size: 5782
KpnI G_GTAC'C
Cuts at: 3107 3107
Size: 5782
MfeI C'AATT_G
Cuts at: 2192 3381 2192
Size: 1189 4593
MluI A'CGCG_T
Cuts at: 5658 5658
Size: 5782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4896 5080 1305
Size: 3591 184 2007
Fragments arranged by size:
3591 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2175 10
Size: 54 1425 343 343 3617
Fragments arranged by size:
3617 1425 343 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3740 4099 4258 518
Size: 648 2574 359 159 2042
Fragments arranged by size:
2574 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2049 2272 2294 2372 2707 3198 3875 5023
Size: 443 223 22 78 335 491 677 1148
Cuts at: 5023 5268 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 491 443 335 245 223
78 22
NaeI GCC'GGC
Cuts at: 2789 2789
Size: 5782
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5230
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5782
NgoMIV G'CCGG_C
Cuts at: 2787 2787
Size: 5782
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5782
NotI GC'GGCC_GC
Cuts at: 2760 3229 2760
Size: 469 5313
NspI r_CATG'y
Cuts at: 1132 1229 3140 4685 1132
Size: 97 1911 1545 2229
Fragments arranged by size:
2229 1911 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2893 1348
Size: 1545 4237
PciI A'CATG_T
Cuts at: 4681 4681
Size: 5782
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2079 1108
Size: 971 4811
PmeI GTTT'AAAC
Cuts at: 3086 3086
Size: 5782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3851 3883 5364 5396 3851
Size: 32 1481 32 4237
Fragments arranged by size:
4237 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2455 2676 2833 2455
Size: 221 157 5404
Fragments arranged by size:
5404 221 157
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5782
PsiI TTA'TAA
Cuts at: 3352 3352
Size: 5782
PstI C_TGCA'G
Cuts at: 830 2435 2684 2706 3134 830
Size: 1605 249 22 428 3478
Fragments arranged by size:
3478 1605 428 249 22
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5782
PvuII CAG'CTG
Cuts at: 2049 2707 2049
Size: 658 5124
SacI G_AGCT'C
Cuts at: 2418 2952 2418
Size: 534 5248
SacII CC_GC'GG
Cuts at: 2373 2373
Size: 5782
SalI G'TCGA_C
Cuts at: 1936 3124 1936
Size: 1188 4594
SbfI CC_TGCA'GG
Cuts at: 3134 3134
Size: 5782
ScaI AGT'ACT
Cuts at: 4030 5742 4030
Size: 1712 4070
SexAI A'CCwGG_T
Cuts at: 2835 2835
Size: 5782
SfcI C'TryA_G
Cuts at: 826 1045 2431 2680 2702 3130 4265 4946 5137
Size: 219 1386 249 22 428 1135 681 191
Cuts at: 5137 826
Size: 1471
Fragments arranged by size:
1471 1386 1135 681 428 249 219 191
22
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5782
SmaI CCC'GGG
Cuts at: 1872 2161 2465 2540 3109 1872
Size: 289 304 75 569 4545
Fragments arranged by size:
4545 569 304 289 75
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2687 3886 4787 5049 820
Size: 197 926 744 1199 901 262 1553
Fragments arranged by size:
1553 1199 926 901 744 262 197
SnaBI TAC'GTA
Cuts at: 493 3573 493
Size: 3080 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5782
SphI G_CATG'C
Cuts at: 1132 3140 1132
Size: 2008 3774
SspI AAT'ATT
Cuts at: 5 52 3706 5
Size: 47 3654 2081
Fragments arranged by size:
3654 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5782
StyI C'CwwG_G
Cuts at: 513 1065 2451 2520 3221 513
Size: 552 1386 69 701 3074
Fragments arranged by size:
3074 1386 701 552 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2369 2831 3809 3826 3979 4164 4609
Size: 1255 789 462 978 17 153 185 445
Cuts at: 4609 325
Size: 1498
Fragments arranged by size:
1498 1255 978 789 462 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3069 4028 5740
Size: 274 80 33 51 1443 1092 959 1712
Cuts at: 5740 96
Size: 138
Fragments arranged by size:
1712 1443 1092 959 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2459 2491 5742 5774 2459
Size: 32 3251 32 2467
Fragments arranged by size:
3251 2467 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2837 1316
Size: 1521 4261
XbaI T'CTAG_A
Cuts at: 3118 3118
Size: 5782
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2527 2527
Size: 5782
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5782
XmaI C'CCGG_G
Cuts at: 1870 2159 2463 2538 3107 1870
Size: 289 304 75 569 4545
Fragments arranged by size:
4545 569 304 289 75
XmnI GAAnn'nnTTC
Cuts at: 3911 3911
Size: 5782
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3590 276
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SacII SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SanDI SapI
SfiI SfoI SrfI SwaI