Restriction map for
pFN21AE1450
(Circular) (Six-base) MAPSORT of: pFN21AE1450.seq Check: 7363 from: 1 to: 5989
pFN21AE1450 5989 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3799 278
Size: 53 83 186 936 2263 2468
Fragments arranged by size:
2468 2263 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3332 1937
Size: 1395 4594
Acc65I G'GTAC_C
Cuts at: 3310 3310
Size: 5989
AclI AA'CG_TT
Cuts at: 1563 4116 4489 1563
Size: 2553 373 3063
Fragments arranged by size:
3063 2553 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2917 4052 5436 5704 1632
Size: 1285 1135 1384 268 1917
Fragments arranged by size:
1917 1384 1285 1135 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5792
AflIII A'CryG_T
Cuts at: 1203 4888 5865 1203
Size: 3685 977 1327
Fragments arranged by size:
3685 1327 977
AgeI A'CCGG_T
Cuts at: 4820 4820
Size: 5989
AhdI GACnn_n'nnGTC
Cuts at: 4718 4718
Size: 5989
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5989
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5989
AlwNI CAG_nnn'CTG
Cuts at: 3068 3732 5304 3068
Size: 664 1572 3753
Fragments arranged by size:
3753 1572 664
ApaLI G'TGCA_C
Cuts at: 4046 5202 4046
Size: 1156 4833
ApoI r'AATT_y
Cuts at: 1493 2427 3142 3298 3524 3676 5785 1493
Size: 934 715 156 226 152 2109 1697
Fragments arranged by size:
2109 1697 934 715 226 156 152
AseI AT'TA_AT
Cuts at: 160 1032 2189 4543 160
Size: 872 1157 2354 1606
Fragments arranged by size:
2354 1606 1157 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5989
AvaI C'yCGr_G
Cuts at: 1870 1943 3314 1870
Size: 73 1371 4545
Fragments arranged by size:
4545 1371 73
BamHI G'GATC_C
Cuts at: 3319 3686 3319
Size: 367 5622
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3310 4765 618
Size: 325 217 627 1523 1455 1842
Fragments arranged by size:
1842 1523 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3308 1391
Size: 1917 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5018
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4180 4214 1903
Size: 34 2243 34 3678
Fragments arranged by size:
3678 2243 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3881 5091 3881
Size: 1210 4779
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5989
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4600 136
Size: 107 122 71 631 605 2928 1525
Fragments arranged by size:
2928 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5984 5984
Size: 5989
BlpI GC'TnA_GC
Cuts at: 3083 3406 3083
Size: 323 5666
Bme1580I G_kGCm'C
Cuts at: 1790 4050 5206 1790
Size: 2260 1156 2573
Fragments arranged by size:
2573 2260 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5989
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3720 4678 442
Size: 416 53 83 399 24 2303 958 1753
Fragments arranged by size:
2303 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5989
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2429 2435 2746 3045 4649 1239
Size: 1190 6 311 299 1604 2579
Fragments arranged by size:
2579 1604 1190 311 299 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2909 4114 4979 5277 2909
Size: 1205 865 298 3621
Fragments arranged by size:
3621 1205 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4652 882
Size: 617 3153 2219
Fragments arranged by size:
3153 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3780 493
Size: 711 2576 2702
Fragments arranged by size:
2702 2576 711
BsaBI GATnn'nnATC
Cuts at: 5983 5983
Size: 5989
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3796 4178 4813 275
Size: 53 83 186 936 2263 382 635 1451
Fragments arranged by size:
2263 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4422 4820 5094 5241 5652 4422
Size: 398 274 147 411 4759
Fragments arranged by size:
4759 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5764
Fragments arranged by size:
5764 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2267 2761 3263 1186
Size: 1081 494 502 3912
Fragments arranged by size:
3912 1081 502 494
BseYI C'CCAG_C
Cuts at: 2906 3078 5192 5688 2906
Size: 172 2114 496 3207
Fragments arranged by size:
3207 2114 496 172
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3093 1163
Size: 573 1357 4059
Fragments arranged by size:
4059 1357 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3439 4200 5228 1540
Size: 275 186 1438 761 1028 2301
Fragments arranged by size:
2301 1438 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4050 4135 5206 4050
Size: 85 1071 4833
Fragments arranged by size:
4833 1071 85
BsmI GAATG_Cn'
Cuts at: 2403 2751 3500 3593 2403
Size: 348 749 93 4799
Fragments arranged by size:
4799 749 348 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2653 2653
Size: 5989
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3308 4050 4135 5206 1391
Size: 399 1518 742 85 1071 2174
Fragments arranged by size:
2174 1518 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5652 5652
Size: 5989
BspHI T'CATG_A
Cuts at: 3878 5608 3878
Size: 1730 4259
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2031 3344 844
Size: 1187 1313 3489
Fragments arranged by size:
3489 1313 1187
BsrBI CCG'CTC
Cuts at: 1394 1823 3876 1394
Size: 429 2053 3507
Fragments arranged by size:
3507 2053 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2652 4484 4658 65
Size: 1183 1404 1832 174 1396
Fragments arranged by size:
1832 1404 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4633 4820 1700
Size: 110 2823 187 2869
Fragments arranged by size:
2869 2823 187 110
BsrGI T'GTAC_A
Cuts at: 96 2089 96
Size: 1993 3996
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5989
BssSI C'ACGA_G
Cuts at: 3173 4049 5061 3173
Size: 876 1012 4101
Fragments arranged by size:
4101 1012 876
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3096 794
Size: 2302 3687
BstBI TT'CG_AA
Cuts at: 4793 4793
Size: 5989
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5989
BstYI r'GATC_y
Cuts at: 1972 2632 3203 3319 3686 4071 4088 5540 5626
Size: 660 571 116 367 385 17 1452 86
Cuts at: 5626 5638 5984 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 660 571 385 367 346 116
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5437
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5250
Fragments arranged by size:
5250 683 56
BtsI GCAGTG_nn'
Cuts at: 3505 4299 4319 3505
Size: 794 20 5175
Fragments arranged by size:
5175 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5989
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5954
DraI TTT'AAA
Cuts at: 3293 3293
Size: 5989
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4996 809
Size: 507 3680 1802
Fragments arranged by size:
3680 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2482 3436 4325
Size: 54 1425 50 275 18 652 954 889
Cuts at: 4325 5954 8
Size: 1629 43
Fragments arranged by size:
1629 1425 954 889 652 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3436 1537
Size: 275 1624 4090
Fragments arranged by size:
4090 1624 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2096 2990 3919 1353
Size: 743 894 929 3423
Fragments arranged by size:
3423 929 894 743
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4576 4950 5096 387
Size: 1064 138 2987 374 146 1280
Fragments arranged by size:
2987 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2429 2435 2746 2917 3045 4052 4649
Size: 393 797 6 311 171 128 1007 597
Cuts at: 4649 5436 5704 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1007 797 787 597 393 311 268
171 128 6
EcoRI G'AATT_C
Cuts at: 2427 3298 3676 2427
Size: 871 378 4740
Fragments arranged by size:
4740 871 378
EcoRV GAT'ATC
Cuts at: 2551 2551
Size: 5989
FspI TGC'GCA
Cuts at: 4495 4495
Size: 5989
HaeII r_GCGC'y
Cuts at: 1678 5136 1678
Size: 3458 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2125 2157 4630 4662 4704
Size: 32 135 32 553 32 2473 32 42
Cuts at: 4704 4736 1373
Size: 32 2626
Fragments arranged by size:
2626 2473 553 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2613 3333 3579 669
Size: 988 281 675 720 246 3079
Fragments arranged by size:
3079 988 720 675 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5989
HpaI GTT'AAC
Cuts at: 2613 3579 2613
Size: 966 5023
KpnI G_GTAC'C
Cuts at: 3314 3314
Size: 5989
MfeI C'AATT_G
Cuts at: 2046 3588 2046
Size: 1542 4447
MluI A'CGCG_T
Cuts at: 5865 5865
Size: 5989
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2289 5103 5287 1305
Size: 984 2814 184 2007
Fragments arranged by size:
2814 2007 984 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2484 10
Size: 54 1425 343 652 3515
Fragments arranged by size:
3515 1425 652 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2964 3947 4306 4465 518
Size: 648 1798 983 359 159 2042
Fragments arranged by size:
2042 1798 983 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2018 3405 4082 5230 5475 1606
Size: 412 1387 677 1148 245 2120
Fragments arranged by size:
2120 1387 1148 677 412 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5437
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5989
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5989
NotI GC'GGCC_GC
Cuts at: 3436 3436
Size: 5989
NspI r_CATG'y
Cuts at: 1132 1229 2502 3347 4892 1132
Size: 97 1273 845 1545 2229
Fragments arranged by size:
2229 1545 1273 845 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5989
PciI A'CATG_T
Cuts at: 4888 4888
Size: 5989
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3068 1108
Size: 1960 4029
PmeI GTTT'AAAC
Cuts at: 3293 3293
Size: 5989
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4058 4090 5571 5603 4058
Size: 32 1481 32 4444
Fragments arranged by size:
4444 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2665 1613
Size: 1052 4937
PsiI TTA'TAA
Cuts at: 3559 3559
Size: 5989
PstI C_TGCA'G
Cuts at: 830 2700 3341 830
Size: 1870 641 3478
Fragments arranged by size:
3478 1870 641
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5989
SalI G'TCGA_C
Cuts at: 1936 3331 1936
Size: 1395 4594
SbfI CC_TGCA'GG
Cuts at: 3341 3341
Size: 5989
ScaI AGT'ACT
Cuts at: 4237 5949 4237
Size: 1712 4277
SfcI C'TryA_G
Cuts at: 826 1045 2586 2696 3337 4472 5153 5344 826
Size: 219 1541 110 641 1135 681 191 1471
Fragments arranged by size:
1541 1471 1135 681 641 219 191 110
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5989
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5989
SmaI CCC'GGG
Cuts at: 1872 3316 1872
Size: 1444 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2888 4093 4994 5256 820
Size: 197 926 945 1205 901 262 1553
Fragments arranged by size:
1553 1205 945 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3780 493
Size: 3287 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5989
SphI G_CATG'C
Cuts at: 1132 3347 1132
Size: 2215 3774
SspI AAT'ATT
Cuts at: 5 52 3010 3913 5
Size: 47 2958 903 2081
Fragments arranged by size:
2958 2081 903 47
StuI AGG'CCT
Cuts at: 1517 2736 3104 1517
Size: 1219 368 4402
Fragments arranged by size:
4402 1219 368
StyI C'CwwG_G
Cuts at: 513 1065 3428 513
Size: 552 2363 3074
Fragments arranged by size:
3074 2363 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4016 4033 4186 4371 4816 325
Size: 1255 2436 17 153 185 445 1498
Fragments arranged by size:
2436 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2089 2457 4235
Size: 274 80 33 51 1443 112 368 1778
Cuts at: 4235 5947 96
Size: 1712 138
Fragments arranged by size:
1778 1712 1443 368 274 138 112 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5949 5981 5949
Size: 32 5957
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5989
XbaI T'CTAG_A
Cuts at: 3325 3325
Size: 5989
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5989
XmaI C'CCGG_G
Cuts at: 1870 3314 1870
Size: 1444 4545
XmnI GAAnn'nnTTC
Cuts at: 2427 4118 2427
Size: 1691 4298
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3797 276
Size: 53 83 186 936 2263 2468
Fragments arranged by size:
2468 2263 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI