Restriction map for
pFN21AE1447
(Circular) (Six-base) MAPSORT of: pFN21AE1447.seq Check: 1004 from: 1 to: 5581
pFN21AE1447 5581 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2183 2183
Size: 5581
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3391 278
Size: 53 83 186 936 1855 2468
Fragments arranged by size:
2468 1855 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2924 1937
Size: 987 4594
Acc65I G'GTAC_C
Cuts at: 2492 2902 2492
Size: 410 5171
AclI AA'CG_TT
Cuts at: 1563 3708 4081 1563
Size: 2145 373 3063
Fragments arranged by size:
3063 2145 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3644 5028 5296 1632
Size: 2012 1384 268 1917
Fragments arranged by size:
2012 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2642 820
Size: 197 1625 3759
Fragments arranged by size:
3759 1625 197
AflIII A'CryG_T
Cuts at: 1203 4480 5457 1203
Size: 3277 977 1327
Fragments arranged by size:
3277 1327 977
AgeI A'CCGG_T
Cuts at: 4412 4412
Size: 5581
AhdI GACnn_n'nnGTC
Cuts at: 2665 4310 2665
Size: 1645 3936
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5581
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5581
AlwNI CAG_nnn'CTG
Cuts at: 2538 2626 3324 4896 2538
Size: 88 698 1572 3223
Fragments arranged by size:
3223 1572 698 88
ApaLI G'TGCA_C
Cuts at: 3638 4794 3638
Size: 1156 4425
ApoI r'AATT_y
Cuts at: 1493 2105 2211 2471 2813 2890 3116 3268 5377
Size: 612 106 260 342 77 226 152 2109
Cuts at: 5377 1493
Size: 1697
Fragments arranged by size:
2109 1697 612 342 260 226 152 106
77
AseI AT'TA_AT
Cuts at: 160 1032 4135 160
Size: 872 3103 1606
Fragments arranged by size:
3103 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5581
AvaI C'yCGr_G
Cuts at: 1870 1943 2327 2906 1870
Size: 73 384 579 4545
Fragments arranged by size:
4545 579 384 73
AvrII C'CTAG_G
Cuts at: 2635 2635
Size: 5581
BamHI G'GATC_C
Cuts at: 2911 3278 2911
Size: 367 5214
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2492 2902 4357 618
Size: 325 217 627 705 410 1455 1842
Fragments arranged by size:
1842 1455 705 627 410 325 217
BanII G_rGCy'C
Cuts at: 1391 2900 1391
Size: 1509 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4610
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3772 3806 1903
Size: 34 1835 34 3678
Fragments arranged by size:
3678 1835 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3473 4683 3473
Size: 1210 4371
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5581
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4192 136
Size: 107 122 71 631 605 2520 1525
Fragments arranged by size:
2520 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2797 5576 2797
Size: 2779 2802
BlpI GC'TnA_GC
Cuts at: 2998 2998
Size: 5581
Bme1580I G_kGCm'C
Cuts at: 1790 3642 4798 1790
Size: 1852 1156 2573
Fragments arranged by size:
2573 1852 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5581
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3312 4270 442
Size: 416 53 83 399 24 1895 958 1753
Fragments arranged by size:
1895 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5581
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4241 1239
Size: 3002 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3706 4571 4869 3706
Size: 865 298 4418
Fragments arranged by size:
4418 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2709 4244 882
Size: 617 1210 1535 2219
Fragments arranged by size:
2219 1535 1210 617
BsaAI yAC'GTr
Cuts at: 493 1204 3372 493
Size: 711 2168 2702
Fragments arranged by size:
2702 2168 711
BsaBI GATnn'nnATC
Cuts at: 5575 5575
Size: 5581
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3388 3770 4405 275
Size: 53 83 186 936 1855 382 635 1451
Fragments arranged by size:
1855 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2061 4014 4412 4686 4833 5244 2061
Size: 1953 398 274 147 411 2398
Fragments arranged by size:
2398 1953 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5356
Fragments arranged by size:
5356 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2035 1186
Size: 849 4732
BseYI C'CCAG_C
Cuts at: 2458 2538 4784 5280 2458
Size: 80 2246 496 2759
Fragments arranged by size:
2759 2246 496 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2211 1163
Size: 573 475 4533
Fragments arranged by size:
4533 573 475
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3031 3792 4820 1540
Size: 275 186 1030 761 1028 2301
Fragments arranged by size:
2301 1030 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3642 3727 4798 3642
Size: 85 1071 4425
Fragments arranged by size:
4425 1071 85
BsmI GAATG_Cn'
Cuts at: 3092 3185 3092
Size: 93 5488
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2900 3642 3727 4798 1391
Size: 399 1110 742 85 1071 2174
Fragments arranged by size:
2174 1110 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 2061 5244 2061
Size: 3183 2398
BspHI T'CATG_A
Cuts at: 3470 5200 3470
Size: 1730 3851
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2183 2727 2753 2936 844
Size: 1339 544 26 183 3489
Fragments arranged by size:
3489 1339 544 183 26
BsrBI CCG'CTC
Cuts at: 1394 1823 3468 1394
Size: 429 1645 3507
Fragments arranged by size:
3507 1645 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4076 4250 65
Size: 1183 2828 174 1396
Fragments arranged by size:
2828 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4225 4412 1700
Size: 110 2415 187 2869
Fragments arranged by size:
2869 2415 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5581
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5581
BssSI C'ACGA_G
Cuts at: 3641 4653 3641
Size: 1012 4569
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5581
BstBI TT'CG_AA
Cuts at: 4385 4385
Size: 5581
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5581
BstYI r'GATC_y
Cuts at: 1972 2797 2911 3278 3663 3680 5132 5218 5230
Size: 825 114 367 385 17 1452 86 12
Cuts at: 5230 5576 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 825 385 367 346 114 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2038 2082 2673 513
Size: 552 939 34 44 591 3421
Fragments arranged by size:
3421 939 591 552 44 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4842
Fragments arranged by size:
4842 683 56
BtsI GCAGTG_nn'
Cuts at: 2539 3097 3891 3911 2539
Size: 558 794 20 4209
Fragments arranged by size:
4209 794 558 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5581
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5546
DraI TTT'AAA
Cuts at: 2885 2885
Size: 5581
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4588 809
Size: 507 3272 1802
Fragments arranged by size:
3272 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3028 3917 5546
Size: 54 1425 50 275 18 1198 889 1629
Cuts at: 5546 8
Size: 43
Fragments arranged by size:
1629 1425 1198 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3028 1537
Size: 275 1216 4090
Fragments arranged by size:
4090 1216 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3511 1353
Size: 2158 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2199 4168 4542 4688 387
Size: 1064 138 610 1969 374 146 1280
Fragments arranged by size:
1969 1280 1064 610 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3644 4241 5028 5296 1239
Size: 393 2012 597 787 268 1524
Fragments arranged by size:
2012 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2105 2890 3268 2105
Size: 785 378 4418
Fragments arranged by size:
4418 785 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2431 2431
Size: 5581
FspI TGC'GCA
Cuts at: 4087 4087
Size: 5581
HaeII r_GCGC'y
Cuts at: 1678 4728 1678
Size: 3050 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2141 2173 2235 2267 2348
Size: 32 135 32 569 32 62 32 81
Cuts at: 2348 2380 2651 2683 4222 4254 4296 4328 1373
Size: 32 271 32 1539 32 42 32 2626
Fragments arranged by size:
2626 1539 569 271 135 81 62 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2925 3171 669
Size: 988 281 987 246 3079
Fragments arranged by size:
3079 988 987 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5581
HpaI GTT'AAC
Cuts at: 3171 3171
Size: 5581
KpnI G_GTAC'C
Cuts at: 2496 2906 2496
Size: 410 5171
MfeI C'AATT_G
Cuts at: 3180 3180
Size: 5581
MluI A'CGCG_T
Cuts at: 5457 5457
Size: 5581
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2367 4695 4879 1305
Size: 1062 2328 184 2007
Fragments arranged by size:
2328 2007 1062 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3759
Fragments arranged by size:
3759 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2621 3539 3898 4057 518
Size: 648 1455 918 359 159 2042
Fragments arranged by size:
2042 1455 918 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2997 3674 4822 5067 1606
Size: 1391 677 1148 245 2120
Fragments arranged by size:
2120 1391 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2673 513
Size: 552 939 669 3421
Fragments arranged by size:
3421 939 669 552
NdeI CA'TA_TG
Cuts at: 387 2424 387
Size: 2037 3544
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5581
NotI GC'GGCC_GC
Cuts at: 3028 3028
Size: 5581
NspI r_CATG'y
Cuts at: 1132 1229 2101 2939 4484 1132
Size: 97 872 838 1545 2229
Fragments arranged by size:
2229 1545 872 838 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5581
PciI A'CATG_T
Cuts at: 4480 4480
Size: 5581
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5581
PmeI GTTT'AAAC
Cuts at: 2885 2885
Size: 5581
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3650 3682 5163 5195 3650
Size: 32 1481 32 4036
Fragments arranged by size:
4036 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5581
PsiI TTA'TAA
Cuts at: 3151 3151
Size: 5581
PstI C_TGCA'G
Cuts at: 830 2933 830
Size: 2103 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5581
SalI G'TCGA_C
Cuts at: 1936 2923 1936
Size: 987 4594
SbfI CC_TGCA'GG
Cuts at: 2933 2933
Size: 5581
ScaI AGT'ACT
Cuts at: 3829 5541 3829
Size: 1712 3869
SfcI C'TryA_G
Cuts at: 826 1045 2929 4064 4745 4936 826
Size: 219 1884 1135 681 191 1471
Fragments arranged by size:
1884 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5581
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5581
SmaI CCC'GGG
Cuts at: 1872 2908 1872
Size: 1036 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2327 2642 3685 4586 4848 820
Size: 197 926 384 315 1043 901 262 1553
Fragments arranged by size:
1553 1043 926 901 384 315 262 197
SnaBI TAC'GTA
Cuts at: 493 3372 493
Size: 2879 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5581
SphI G_CATG'C
Cuts at: 1132 2101 2939 1132
Size: 969 838 3774
Fragments arranged by size:
3774 969 838
SspI AAT'ATT
Cuts at: 5 52 3505 5
Size: 47 3453 2081
Fragments arranged by size:
3453 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5581
StyI C'CwwG_G
Cuts at: 513 1065 2004 2635 2673 3020 513
Size: 552 939 631 38 347 3074
Fragments arranged by size:
3074 939 631 552 347 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3608 3625 3778 3963 4408 325
Size: 1255 2028 17 153 185 445 1498
Fragments arranged by size:
2028 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3827 5539 96
Size: 274 80 33 51 1443 1850 1712 138
Fragments arranged by size:
1850 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2078 2110 2534 2566 5541 5573 2078
Size: 32 424 32 2975 32 2086
Fragments arranged by size:
2975 2086 424 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5581
XbaI T'CTAG_A
Cuts at: 2917 2917
Size: 5581
XhoI C'TCGA_G
Cuts at: 1943 2327 1943
Size: 384 5197
XmaI C'CCGG_G
Cuts at: 1870 2906 1870
Size: 1036 4545
XmnI GAAnn'nnTTC
Cuts at: 2474 3710 2474
Size: 1236 4345
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3389 276
Size: 53 83 186 936 1855 2468
Fragments arranged by size:
2468 1855 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI