Restriction map for
pFN21AE1446
(Circular) (Six-base) MAPSORT of: pFN21AE1446.seq Check: 4798 from: 1 to: 5680
pFN21AE1446 5680 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3490 278
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3023 1937
Size: 1086 4594
Acc65I G'GTAC_C
Cuts at: 3001 3001
Size: 5680
AclI AA'CG_TT
Cuts at: 1563 3807 4180 1563
Size: 2244 373 3063
Fragments arranged by size:
3063 2244 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3743 5127 5395 1632
Size: 2111 1384 268 1917
Fragments arranged by size:
2111 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5483
AflIII A'CryG_T
Cuts at: 1203 4579 5556 1203
Size: 3376 977 1327
Fragments arranged by size:
3376 1327 977
AgeI A'CCGG_T
Cuts at: 4511 4511
Size: 5680
AhdI GACnn_n'nnGTC
Cuts at: 2691 4409 2691
Size: 1718 3962
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5680
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5680
AlwNI CAG_nnn'CTG
Cuts at: 3423 4995 3423
Size: 1572 4108
ApaLI G'TGCA_C
Cuts at: 3737 4893 3737
Size: 1156 4524
ApoI r'AATT_y
Cuts at: 1493 2963 2989 3215 3367 5476 1493
Size: 1470 26 226 152 2109 1697
Fragments arranged by size:
2109 1697 1470 226 152 26
AseI AT'TA_AT
Cuts at: 160 1032 4234 160
Size: 872 3202 1606
Fragments arranged by size:
3202 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
AvaI C'yCGr_G
Cuts at: 1870 1943 3005 1870
Size: 73 1062 4545
Fragments arranged by size:
4545 1062 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2917 2950 2917
Size: 33 5647
BamHI G'GATC_C
Cuts at: 2389 3010 3377 2389
Size: 621 367 4692
Fragments arranged by size:
4692 621 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2141 3001 4456 618
Size: 325 217 627 354 860 1455 1842
Fragments arranged by size:
1842 1455 860 627 354 325 217
BanII G_rGCy'C
Cuts at: 1391 2418 2514 2999 1391
Size: 1027 96 485 4072
Fragments arranged by size:
4072 1027 485 96
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2681 928
Size: 971 782 3927
Fragments arranged by size:
3927 971 782
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2002 2036 3871 3905 1903
Size: 34 65 34 1835 34 3678
Fragments arranged by size:
3678 1835 65 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2945 3572 4782 2945
Size: 627 1210 3843
Fragments arranged by size:
3843 1210 627
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5680
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4291 136
Size: 107 122 71 631 605 2619 1525
Fragments arranged by size:
2619 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5675 5675
Size: 5680
BlpI GC'TnA_GC
Cuts at: 2215 3097 2215
Size: 882 4798
Bme1580I G_kGCm'C
Cuts at: 1790 2144 3741 4897 1790
Size: 354 1597 1156 2573
Fragments arranged by size:
2573 1597 1156 354
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5680
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2197 3411 4369
Size: 416 53 83 399 24 780 1214 958
Cuts at: 4369 442
Size: 1753
Fragments arranged by size:
1753 1214 958 780 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5680
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2093 2093
Size: 5680
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2556 4340 1239
Size: 1317 1784 2579
Fragments arranged by size:
2579 1784 1317
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2258 3805 4670 4968 2258
Size: 1547 865 298 2970
Fragments arranged by size:
2970 1547 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2064 4343 882
Size: 617 565 2279 2219
Fragments arranged by size:
2279 2219 617 565
BsaAI yAC'GTr
Cuts at: 493 1204 3471 493
Size: 711 2267 2702
Fragments arranged by size:
2702 2267 711
BsaBI GATnn'nnATC
Cuts at: 5674 5674
Size: 5680
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3487 3869 4504 275
Size: 53 83 186 936 1954 382 635 1451
Fragments arranged by size:
1954 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2159 2292 2682 4113 4511 4785 4932 5343 2159
Size: 133 390 1431 398 274 147 411 2496
Fragments arranged by size:
2496 1431 411 398 390 274 147 133
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5455
Fragments arranged by size:
5455 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 1186
Size: 880 4800
BseYI C'CCAG_C
Cuts at: 2137 4883 5379 2137
Size: 2746 496 2438
Fragments arranged by size:
2746 2438 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5107
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3130 3891 4919 1540
Size: 275 186 1129 761 1028 2301
Fragments arranged by size:
2301 1129 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2514 2565 3741 3826 4897 2514
Size: 51 1176 85 1071 3297
Fragments arranged by size:
3297 1176 1071 85 51
BsmI GAATG_Cn'
Cuts at: 3191 3284 3191
Size: 93 5587
BsmBI CGTCTCn'nnnn_
Cuts at: 2240 2240
Size: 5680
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2144 2418 2514 2565 2999 3741 3826
Size: 399 354 274 96 51 434 742 85
Cuts at: 3826 4897 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 434 399 354 274 96
85 51
BspEI T'CCGG_A
Cuts at: 2292 2682 5343 2292
Size: 390 2661 2629
Fragments arranged by size:
2661 2629 390
BspHI T'CATG_A
Cuts at: 3569 5299 3569
Size: 1730 3950
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2306 2391 3035 844
Size: 1462 85 644 3489
Fragments arranged by size:
3489 1462 644 85
BsrBI CCG'CTC
Cuts at: 1394 1823 2035 3567 1394
Size: 429 212 1532 3507
Fragments arranged by size:
3507 1532 429 212
BsrDI GCAATG_nn'
Cuts at: 65 1248 2443 4175 4349 65
Size: 1183 1195 1732 174 1396
Fragments arranged by size:
1732 1396 1195 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4324 4511 1700
Size: 110 2514 187 2869
Fragments arranged by size:
2869 2514 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5680
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5680
BssSI C'ACGA_G
Cuts at: 3740 4752 3740
Size: 1012 4668
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5680
BstBI TT'CG_AA
Cuts at: 2961 4484 2961
Size: 1523 4157
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5680
BstXI CCAn_nnnn'nTGG
Cuts at: 2504 2891 2504
Size: 387 5293
BstYI r'GATC_y
Cuts at: 1972 2389 2427 3010 3377 3762 3779 5231 5317
Size: 417 38 583 367 385 17 1452 86
Cuts at: 5317 5329 5675 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 583 417 385 367 346 86
38 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2337 2760 513
Size: 552 1272 423 3433
Fragments arranged by size:
3433 1272 552 423
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2473 487
Size: 683 56 1247 3694
Fragments arranged by size:
3694 1247 683 56
BtsI GCAGTG_nn'
Cuts at: 3196 3990 4010 3196
Size: 794 20 4866
Fragments arranged by size:
4866 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5680
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2644 2679 2770 2805 529
Size: 35 2080 35 91 35 3404
Fragments arranged by size:
3404 2080 91 35 35 35
DraI TTT'AAA
Cuts at: 2984 2984
Size: 5680
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2102 2735 4687 809
Size: 507 786 633 1952 1802
Fragments arranged by size:
1952 1802 786 633 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2088 2494 3127
Size: 54 1425 50 275 18 258 406 633
Cuts at: 3127 4016 5645 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 633 406 275 258 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3127 1537
Size: 275 1315 4090
Fragments arranged by size:
4090 1315 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2826 2836 3610 1353
Size: 1473 10 774 3423
Fragments arranged by size:
3423 1473 774 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4267 4641 4787 387
Size: 1064 138 2678 374 146 1280
Fragments arranged by size:
2678 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2512 2512
Size: 5680
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2556 3743 4340 5127 5395 1239
Size: 393 924 1187 597 787 268 1524
Fragments arranged by size:
1524 1187 924 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2021 2782 2021
Size: 761 4919
EcoO109I rG'GnC_Cy
Cuts at: 2095 2468 2095
Size: 373 5307
EcoRI G'AATT_C
Cuts at: 2989 3367 2989
Size: 378 5302
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2872 2872
Size: 5680
FspI TGC'GCA
Cuts at: 4186 4186
Size: 5680
HaeII r_GCGC'y
Cuts at: 1678 4827 1678
Size: 3149 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2677 2709 4321 4353 4395
Size: 32 135 32 1105 32 1612 32 42
Cuts at: 4395 4427 1373
Size: 32 2626
Fragments arranged by size:
2626 1612 1105 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2701 3024 3270 669
Size: 988 281 763 323 246 3079
Fragments arranged by size:
3079 988 763 323 281 246
HindIII A'AGCT_T
Cuts at: 748 2642 748
Size: 1894 3786
HpaI GTT'AAC
Cuts at: 3270 3270
Size: 5680
KpnI G_GTAC'C
Cuts at: 3005 3005
Size: 5680
MfeI C'AATT_G
Cuts at: 3279 3279
Size: 5680
MluI A'CGCG_T
Cuts at: 5556 5556
Size: 5680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4794 4978 1305
Size: 3489 184 2007
Fragments arranged by size:
3489 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2090 2496 10
Size: 54 1425 343 258 406 3194
Fragments arranged by size:
3194 1425 406 343 258 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2147 2327 2502 2606 2612 2889 3638
Size: 648 981 180 175 104 6 277 749
Cuts at: 3638 3997 4156 518
Size: 359 159 2042
Fragments arranged by size:
2042 981 749 648 359 277 180 175
159 104 6
MspA1I CmG'CkG
Cuts at: 1606 2219 2229 2263 2708 2910 3096 3773 4921
Size: 613 10 34 445 202 186 677 1148
Cuts at: 4921 5166 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 613 445 245 202 186
34 10
NcoI C'CATG_G
Cuts at: 513 1065 2760 513
Size: 552 1695 3433
Fragments arranged by size:
3433 1695 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5680
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5680
NotI GC'GGCC_GC
Cuts at: 3127 3127
Size: 5680
NspI r_CATG'y
Cuts at: 1132 1229 2204 3038 4583 1132
Size: 97 975 834 1545 2229
Fragments arranged by size:
2229 1545 975 834 97
PasI CC'CwG_GG
Cuts at: 1348 2473 1348
Size: 1125 4555
PciI A'CATG_T
Cuts at: 4579 4579
Size: 5680
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5680
PmeI GTTT'AAAC
Cuts at: 2984 2984
Size: 5680
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2792 2824 3749 3781 5262 5294 2792
Size: 32 925 32 1481 32 3178
Fragments arranged by size:
3178 1481 925 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2095 2468 2095
Size: 373 5307
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5680
PsiI TTA'TAA
Cuts at: 3250 3250
Size: 5680
PstI C_TGCA'G
Cuts at: 830 2025 3032 830
Size: 1195 1007 3478
Fragments arranged by size:
3478 1195 1007
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5680
PvuII CAG'CTG
Cuts at: 2219 2229 2263 2708 2219
Size: 10 34 445 5191
Fragments arranged by size:
5191 445 34 10
SacI G_AGCT'C
Cuts at: 2514 2514
Size: 5680
SalI G'TCGA_C
Cuts at: 1936 3022 1936
Size: 1086 4594
SanDI GG'GwC_CC
Cuts at: 2468 2468
Size: 5680
SbfI CC_TGCA'GG
Cuts at: 3032 3032
Size: 5680
ScaI AGT'ACT
Cuts at: 2957 3928 5640 2957
Size: 971 1712 2997
Fragments arranged by size:
2997 1712 971
SfcI C'TryA_G
Cuts at: 826 1045 2021 2902 3028 4163 4844 5035 826
Size: 219 976 881 126 1135 681 191 1471
Fragments arranged by size:
1471 1135 976 881 681 219 191 126
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5680
SmaI CCC'GGG
Cuts at: 1872 3007 1872
Size: 1135 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2273 3784 4685 4947 820
Size: 197 926 330 1511 901 262 1553
Fragments arranged by size:
1553 1511 926 901 330 262 197
SnaBI TAC'GTA
Cuts at: 493 3471 493
Size: 2978 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5680
SphI G_CATG'C
Cuts at: 1132 3038 1132
Size: 1906 3774
SspI AAT'ATT
Cuts at: 5 52 3604 5
Size: 47 3552 2081
Fragments arranged by size:
3552 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5680
StyI C'CwwG_G
Cuts at: 513 1065 2091 2625 2760 3119 513
Size: 552 1026 534 135 359 3074
Fragments arranged by size:
3074 1026 552 534 359 135
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3707 3724 3877 4062 4507 325
Size: 1255 2127 17 153 185 445 1498
Fragments arranged by size:
2127 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2955 3926 5638
Size: 274 80 33 51 1443 978 971 1712
Cuts at: 5638 96
Size: 138
Fragments arranged by size:
1712 1443 978 971 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5640 5672 5640
Size: 32 5648
Tth111I GACn'n_nGTC
Cuts at: 1316 2620 1316
Size: 1304 4376
XbaI T'CTAG_A
Cuts at: 3016 3016
Size: 5680
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5680
XmaI C'CCGG_G
Cuts at: 1870 3005 1870
Size: 1135 4545
XmnI GAAnn'nnTTC
Cuts at: 2678 3809 2678
Size: 1131 4549
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3488 276
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacII SapI
SexAI SfiI SfoI SrfI SwaI XcmI