Restriction map for
pFN21AE1441
(Circular) (Six-base) MAPSORT of: pFN21AE1441.seq Check: 8143 from: 1 to: 5941
pFN21AE1441 5941 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2382 3751 278
Size: 53 83 186 936 846 1369 2468
Fragments arranged by size:
2468 1369 936 846 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2382 2808 3284 1937
Size: 445 426 476 4594
Fragments arranged by size:
4594 476 445 426
Acc65I G'GTAC_C
Cuts at: 3262 3262
Size: 5941
AclI AA'CG_TT
Cuts at: 1563 4068 4441 1563
Size: 2505 373 3063
Fragments arranged by size:
3063 2505 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3058 3140 4004 5388 5656 1632
Size: 1426 82 864 1384 268 1917
Fragments arranged by size:
1917 1426 1384 864 268 82
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5744
AflIII A'CryG_T
Cuts at: 1203 4840 5817 1203
Size: 3637 977 1327
Fragments arranged by size:
3637 1327 977
AgeI A'CCGG_T
Cuts at: 4772 4772
Size: 5941
AhdI GACnn_n'nnGTC
Cuts at: 4670 4670
Size: 5941
AleI CACnn'nnGTG
Cuts at: 1166 2410 2412 2801 1166
Size: 1244 2 389 4306
Fragments arranged by size:
4306 1244 389 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2776 1184
Size: 1592 4349
AlwNI CAG_nnn'CTG
Cuts at: 2707 2860 2911 3684 5256 2707
Size: 153 51 773 1572 3392
Fragments arranged by size:
3392 1572 773 153 51
ApaI G_GGCC'C
Cuts at: 3192 3192
Size: 5941
ApaLI G'TGCA_C
Cuts at: 3998 5154 3998
Size: 1156 4785
ApoI r'AATT_y
Cuts at: 1493 3250 3476 3628 5737 1493
Size: 1757 226 152 2109 1697
Fragments arranged by size:
2109 1757 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4495 160
Size: 872 3463 1606
Fragments arranged by size:
3463 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
AvaI C'yCGr_G
Cuts at: 1870 1943 2606 3266 1870
Size: 73 663 660 4545
Fragments arranged by size:
4545 663 660 73
BamHI G'GATC_C
Cuts at: 3271 3638 3271
Size: 367 5574
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2530 2593 2636 2760 3168
Size: 325 217 627 743 63 43 124 408
Cuts at: 3168 3262 4717 618
Size: 94 1455 1842
Fragments arranged by size:
1842 1455 743 627 408 325 217 124
94 63 43
BanII G_rGCy'C
Cuts at: 1391 2015 3192 3260 1391
Size: 624 1177 68 4072
Fragments arranged by size:
4072 1177 624 68
BbeI G_GCGC'C
Cuts at: 2534 3172 2534
Size: 638 5303
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4970
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3182 3216 4132 4166 1903
Size: 34 1245 34 916 34 3678
Fragments arranged by size:
3678 1245 916 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3833 5043 3833
Size: 1210 4731
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5941
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3196 4552 136
Size: 107 122 71 631 605 1524 1356 1525
Fragments arranged by size:
1525 1524 1356 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5936 5936
Size: 5941
BlpI GC'TnA_GC
Cuts at: 3358 3358
Size: 5941
Bme1580I G_kGCm'C
Cuts at: 1790 2565 3192 4002 5158 1790
Size: 775 627 810 1156 2573
Fragments arranged by size:
2573 1156 810 775 627
BmgBI CAC'GTC
Cuts at: 1318 2887 1318
Size: 1569 4372
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3043 3672 4630
Size: 416 53 83 399 24 1626 629 958
Cuts at: 4630 442
Size: 1753
Fragments arranged by size:
1753 1626 958 629 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2394 2420 2906 4601 1239
Size: 1155 26 486 1695 2579
Fragments arranged by size:
2579 1695 1155 486 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4066 4931 5229 4066
Size: 865 298 4778
Fragments arranged by size:
4778 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2894 3208 4604 882
Size: 617 1395 314 1396 2219
Fragments arranged by size:
2219 1396 1395 617 314
BsaAI yAC'GTr
Cuts at: 493 1204 3732 493
Size: 711 2528 2702
Fragments arranged by size:
2702 2528 711
BsaBI GATnn'nnATC
Cuts at: 5935 5935
Size: 5941
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2201 2379 2531 2826
Size: 53 83 186 936 668 178 152 295
Cuts at: 2826 3066 3169 3748 4130 4765 275
Size: 240 103 579 382 635 1451
Fragments arranged by size:
1451 936 668 635 579 382 295 240
186 178 152 103 83 53
BsaWI w'CCGG_w
Cuts at: 4374 4772 5046 5193 5604 4374
Size: 398 274 147 411 4711
Fragments arranged by size:
4711 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2369 2399 3095 3125 1178
Size: 30 165 30 966 30 696 30 3994
Fragments arranged by size:
3994 966 696 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2360 2614 2912 3146 1186
Size: 1174 254 298 234 3981
Fragments arranged by size:
3981 1174 298 254 234
BseYI C'CCAG_C
Cuts at: 2428 2520 5144 5640 2428
Size: 92 2624 496 2729
Fragments arranged by size:
2729 2624 496 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2660 1163
Size: 573 924 4444
Fragments arranged by size:
4444 924 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2497 2668 3391 4152 5180 1540
Size: 275 186 496 171 723 761 1028 2301
Fragments arranged by size:
2301 1028 761 723 496 275 186 171
BsiHKAI G_wGCw'C
Cuts at: 2922 2952 2996 4002 4087 5158 2922
Size: 30 44 1006 85 1071 3705
Fragments arranged by size:
3705 1071 1006 85 44 30
BsmI GAATG_Cn'
Cuts at: 2753 3452 3545 2753
Size: 699 93 5149
Fragments arranged by size:
5149 699 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2370 2370
Size: 5941
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2565 2922 2952 2996 3192 3260
Size: 399 225 550 357 30 44 196 68
Cuts at: 3260 4002 4087 5158 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 550 399 357 225 196
85 68 44 30
BspEI T'CCGG_A
Cuts at: 5604 5604
Size: 5941
BspHI T'CATG_A
Cuts at: 3830 5560 3830
Size: 1730 4211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3296 844
Size: 2452 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2187 2664 3828 1394
Size: 429 364 477 1164 3507
Fragments arranged by size:
3507 1164 477 429 364
BsrDI GCAATG_nn'
Cuts at: 65 1248 2291 4436 4610 65
Size: 1183 1043 2145 174 1396
Fragments arranged by size:
2145 1396 1183 1043 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3056 4585 4772 1700
Size: 110 1246 1529 187 2869
Fragments arranged by size:
2869 1529 1246 187 110
BsrGI T'GTAC_A
Cuts at: 96 2318 2847 96
Size: 2222 529 3190
Fragments arranged by size:
3190 2222 529
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5941
BssSI C'ACGA_G
Cuts at: 4001 5013 4001
Size: 1012 4929
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5941
BstBI TT'CG_AA
Cuts at: 4745 4745
Size: 5941
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5941
BstYI r'GATC_y
Cuts at: 1972 2508 3271 3638 4023 4040 5492 5578 5590
Size: 536 763 367 385 17 1452 86 12
Cuts at: 5590 5936 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 763 536 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2642 2642
Size: 5941
BtgI C'CryG_G
Cuts at: 513 1065 2004 2279 2304 2526 2589 513
Size: 552 939 275 25 222 63 3865
Fragments arranged by size:
3865 939 552 275 222 63 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5202
Fragments arranged by size:
5202 683 56
BtsI GCAGTG_nn'
Cuts at: 2098 2131 2487 2677 3457 4251 4271 2098
Size: 33 356 190 780 794 20 3768
Fragments arranged by size:
3768 794 780 356 190 33 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5941
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5906
DraI TTT'AAA
Cuts at: 3245 3245
Size: 5941
DraIII CAC_nnn'GTG
Cuts at: 2878 2878
Size: 5941
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4948 809
Size: 507 3632 1802
Fragments arranged by size:
3632 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2073 2361 2416
Size: 54 1425 50 275 18 243 288 55
Cuts at: 2416 2665 2691 3388 4277 5906 8
Size: 249 26 697 889 1629 43
Fragments arranged by size:
1629 1425 889 697 288 275 249 243
55 54 50 43 26 18
EagI C'GGCC_G
Cuts at: 1537 1812 2665 3388 1537
Size: 275 853 723 4090
Fragments arranged by size:
4090 853 723 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2114 3871 1353
Size: 761 1757 3423
Fragments arranged by size:
3423 1757 761
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4528 4902 5048 387
Size: 1064 138 2939 374 146 1280
Fragments arranged by size:
2939 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2394 2420 2906 3058 3140 4004 4601
Size: 393 762 26 486 152 82 864 597
Cuts at: 4601 5388 5656 1239
Size: 787 268 1524
Fragments arranged by size:
1524 864 787 762 597 486 393 268
152 82 26
EcoO109I rG'GnC_Cy
Cuts at: 2722 2722
Size: 5941
EcoRI G'AATT_C
Cuts at: 3250 3628 3250
Size: 378 5563
FspI TGC'GCA
Cuts at: 4447 4447
Size: 5941
HaeII r_GCGC'y
Cuts at: 1678 2534 2783 3172 5088 1678
Size: 856 249 389 1916 2531
Fragments arranged by size:
2531 1916 856 389 249
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2369 2401 4582 4614 4656
Size: 32 135 32 797 32 2181 32 42
Cuts at: 4656 4688 1373
Size: 32 2626
Fragments arranged by size:
2626 2181 797 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3285 3531 669
Size: 988 281 1347 246 3079
Fragments arranged by size:
3079 1347 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5941
HpaI GTT'AAC
Cuts at: 3531 3531
Size: 5941
KasI G'GCGC_C
Cuts at: 2530 3168 2530
Size: 638 5303
KpnI G_GTAC'C
Cuts at: 3266 3266
Size: 5941
MfeI C'AATT_G
Cuts at: 3540 3540
Size: 5941
MluI A'CGCG_T
Cuts at: 5817 5817
Size: 5941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5055 5239 1305
Size: 3750 184 2007
Fragments arranged by size:
3750 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2075 2693 10
Size: 54 1425 343 243 618 3258
Fragments arranged by size:
3258 1425 618 343 243 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2284 2410 2412 2758 2801 3899 4258
Size: 648 1118 126 2 346 43 1098 359
Cuts at: 4258 4417 518
Size: 159 2042
Fragments arranged by size:
2042 1118 1098 648 359 346 159 126
43 2
MspA1I CmG'CkG
Cuts at: 1606 2520 2528 2674 2821 2860 3166 3357 4034
Size: 914 8 146 147 39 306 191 677
Cuts at: 4034 5182 5427 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 914 677 306 245 191 147
146 39 8
NaeI GCC'GGC
Cuts at: 3058 3058
Size: 5941
NarI GG'CG_CC
Cuts at: 2531 3169 2531
Size: 638 5303
NcoI C'CATG_G
Cuts at: 513 1065 2004 2279 513
Size: 552 939 275 4175
Fragments arranged by size:
4175 939 552 275
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5941
NgoMIV G'CCGG_C
Cuts at: 3056 3056
Size: 5941
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5941
NotI GC'GGCC_GC
Cuts at: 2665 3388 2665
Size: 723 5218
NspI r_CATG'y
Cuts at: 1132 1229 3203 3299 4844 1132
Size: 97 1974 96 1545 2229
Fragments arranged by size:
2229 1974 1545 97 96
PasI CC'CwG_GG
Cuts at: 1348 2905 1348
Size: 1557 4384
PciI A'CATG_T
Cuts at: 4840 4840
Size: 5941
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5941
PfoI T'CCnGG_A
Cuts at: 2744 2922 2744
Size: 178 5763
PmeI GTTT'AAAC
Cuts at: 3245 3245
Size: 5941
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4010 4042 5523 5555 4010
Size: 32 1481 32 4396
Fragments arranged by size:
4396 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2437 1613
Size: 824 5117
PsiI TTA'TAA
Cuts at: 3511 3511
Size: 5941
PspOMI G'GGCC_C
Cuts at: 3188 3188
Size: 5941
PstI C_TGCA'G
Cuts at: 830 2126 2134 2703 3144 3293 830
Size: 1296 8 569 441 149 3478
Fragments arranged by size:
3478 1296 569 441 149 8
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5941
PvuII CAG'CTG
Cuts at: 2520 2674 2821 2860 2520
Size: 154 147 39 5601
Fragments arranged by size:
5601 154 147 39
SacII CC_GC'GG
Cuts at: 2529 2529
Size: 5941
SalI G'TCGA_C
Cuts at: 1936 3283 1936
Size: 1347 4594
SbfI CC_TGCA'GG
Cuts at: 3293 3293
Size: 5941
ScaI AGT'ACT
Cuts at: 4189 5901 4189
Size: 1712 4229
SfcI C'TryA_G
Cuts at: 826 1045 2122 2130 2699 2953 3140 3289 4424
Size: 219 1077 8 569 254 187 149 1135
Cuts at: 4424 5105 5296 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1077 681 569 254 219 191
187 149 8
SfoI GGC'GCC
Cuts at: 2532 3170 2532
Size: 638 5303
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5941
SmaI CCC'GGG
Cuts at: 1872 2608 3268 1872
Size: 736 660 4545
Fragments arranged by size:
4545 736 660
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4045 4946 5208 820
Size: 197 926 2102 901 262 1553
Fragments arranged by size:
2102 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3732 493
Size: 3239 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5941
SphI G_CATG'C
Cuts at: 1132 3203 3299 1132
Size: 2071 96 3774
Fragments arranged by size:
3774 2071 96
SrfI GCCC'GGGC
Cuts at: 2608 2608
Size: 5941
SspI AAT'ATT
Cuts at: 5 52 3865 5
Size: 47 3813 2081
Fragments arranged by size:
3813 2081 47
StuI AGG'CCT
Cuts at: 1517 2959 1517
Size: 1442 4499
StyI C'CwwG_G
Cuts at: 513 1065 2004 2172 2279 3380 513
Size: 552 939 168 107 1101 3074
Fragments arranged by size:
3074 1101 939 552 168 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2905 3968 3985 4138 4323 4768 325
Size: 1255 1325 1063 17 153 185 445 1498
Fragments arranged by size:
1498 1325 1255 1063 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2318 2655 2847
Size: 274 80 33 51 1443 341 337 192
Cuts at: 2847 4187 5899 96
Size: 1340 1712 138
Fragments arranged by size:
1712 1443 1340 341 337 274 192 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5901 5933 5901
Size: 32 5909
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5941
XbaI T'CTAG_A
Cuts at: 2422 3277 2422
Size: 855 5086
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5941
XmaI C'CCGG_G
Cuts at: 1870 2606 3266 1870
Size: 736 660 4545
Fragments arranged by size:
4545 736 660
XmnI GAAnn'nnTTC
Cuts at: 4070 4070
Size: 5941
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2380 3749 276
Size: 53 83 186 936 846 1369 2468
Fragments arranged by size:
2468 1369 936 846 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I EcoICRI EcoNI EcoRV
FalI FseI FspAI NruI NsiI PacI PmlI PpuMI
PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI
SwaI XcmI