Restriction map for
pFN21AE1438
(Circular) (Six-base) MAPSORT of: pFN21AE1438.seq Check: 1324 from: 1 to: 5590
pFN21AE1438 5590 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2671 2671
Size: 5590
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2619 3400 278
Size: 53 83 186 936 1083 781 2468
Fragments arranged by size:
2468 1083 936 781 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2933 1937
Size: 996 4594
Acc65I G'GTAC_C
Cuts at: 2911 2911
Size: 5590
AclI AA'CG_TT
Cuts at: 1563 3717 4090 1563
Size: 2154 373 3063
Fragments arranged by size:
3063 2154 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2227 2300 3653 5037 5305 1632
Size: 595 73 1353 1384 268 1917
Fragments arranged by size:
1917 1384 1353 595 268 73
AfeI AGC'GCT
Cuts at: 2300 2300
Size: 5590
AflII C'TTAA_G
Cuts at: 820 1017 2291 820
Size: 197 1274 4119
Fragments arranged by size:
4119 1274 197
AflIII A'CryG_T
Cuts at: 1203 4489 5466 1203
Size: 3286 977 1327
Fragments arranged by size:
3286 1327 977
AgeI A'CCGG_T
Cuts at: 4421 4421
Size: 5590
AhdI GACnn_n'nnGTC
Cuts at: 4319 4319
Size: 5590
AleI CACnn'nnGTG
Cuts at: 1166 2210 1166
Size: 1044 4546
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5590
AlwNI CAG_nnn'CTG
Cuts at: 3333 4905 3333
Size: 1572 4018
ApaLI G'TGCA_C
Cuts at: 2568 3647 4803 2568
Size: 1079 1156 3355
Fragments arranged by size:
3355 1156 1079
ApoI r'AATT_y
Cuts at: 1493 2899 3125 3277 5386 1493
Size: 1406 226 152 2109 1697
Fragments arranged by size:
2109 1697 1406 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4144 160
Size: 872 3112 1606
Fragments arranged by size:
3112 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5590
AvaI C'yCGr_G
Cuts at: 1870 1943 2915 1870
Size: 73 972 4545
Fragments arranged by size:
4545 972 73
BamHI G'GATC_C
Cuts at: 2920 3287 2920
Size: 367 5223
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2683 2911 4366 618
Size: 325 217 627 896 228 1455 1842
Fragments arranged by size:
1842 1455 896 627 325 228 217
BanII G_rGCy'C
Cuts at: 1391 2058 2322 2909 1391
Size: 667 264 587 4072
Fragments arranged by size:
4072 667 587 264
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4619
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2485 2519 3781 3815 1903
Size: 34 548 34 1262 34 3678
Fragments arranged by size:
3678 1262 548 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3482 4692 3482
Size: 1210 4380
BclI T'GATC_A
Cuts at: 1548 2530 1548
Size: 982 4608
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2172 2703 4201
Size: 107 122 71 631 605 500 531 1498
Cuts at: 4201 136
Size: 1525
Fragments arranged by size:
1525 1498 631 605 531 500 122 107
71
BglII A'GATC_T
Cuts at: 5585 5585
Size: 5590
BlpI GC'TnA_GC
Cuts at: 3007 3007
Size: 5590
Bme1580I G_kGCm'C
Cuts at: 1790 2382 2572 3651 4807 1790
Size: 592 190 1079 1156 2573
Fragments arranged by size:
2573 1156 1079 592 190
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5590
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2217 2377 3321
Size: 416 53 83 399 24 800 160 944
Cuts at: 3321 4279 442
Size: 958 1753
Fragments arranged by size:
1753 958 944 800 416 399 160 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5590
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4250 1239
Size: 3011 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2737 2861 3715 4580 4878 2737
Size: 124 854 865 298 3449
Fragments arranged by size:
3449 865 854 298 124
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4253 882
Size: 617 2754 2219
Fragments arranged by size:
2754 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2872 3381 493
Size: 711 1668 509 2702
Fragments arranged by size:
2702 1668 711 509
BsaBI GATnn'nnATC
Cuts at: 5584 5584
Size: 5590
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2616 3397 3779 4414
Size: 53 83 186 936 1083 781 382 635
Cuts at: 4414 275
Size: 1451
Fragments arranged by size:
1451 1083 936 781 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2022 4023 4421 4695 4842 5253 2022
Size: 2001 398 274 147 411 2359
Fragments arranged by size:
2359 2001 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5365
Fragments arranged by size:
5365 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5590
BseYI C'CCAG_C
Cuts at: 4793 5289 4793
Size: 496 5094
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2699 1163
Size: 573 963 4054
Fragments arranged by size:
4054 963 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2049 3040 3801 4829 1540
Size: 275 186 48 991 761 1028 2301
Fragments arranged by size:
2301 1028 991 761 275 186 48
BsiHKAI G_wGCw'C
Cuts at: 2190 2472 2572 2877 3651 3736 4807 2190
Size: 282 100 305 774 85 1071 2973
Fragments arranged by size:
2973 1071 774 305 282 100 85
BsmI GAATG_Cn'
Cuts at: 3101 3194 3101
Size: 93 5497
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2058 2190 2322 2382 2472 2572 2877
Size: 399 268 132 132 60 90 100 305
Cuts at: 2877 2909 3651 3736 4807 1391
Size: 32 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 305 268 132 132
100 90 85 60 32
BspEI T'CCGG_A
Cuts at: 5253 5253
Size: 5590
BspHI T'CATG_A
Cuts at: 3479 5209 3479
Size: 1730 3860
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2671 2945 844
Size: 1827 274 3489
Fragments arranged by size:
3489 1827 274
BsrBI CCG'CTC
Cuts at: 1394 1823 2031 3477 1394
Size: 429 208 1446 3507
Fragments arranged by size:
3507 1446 429 208
BsrDI GCAATG_nn'
Cuts at: 65 1248 2490 4085 4259 65
Size: 1183 1242 1595 174 1396
Fragments arranged by size:
1595 1396 1242 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2307 2472 4234 4421 1700
Size: 110 497 165 1762 187 2869
Fragments arranged by size:
2869 1762 497 187 165 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5590
BssHII G'CGCG_C
Cuts at: 1925 2087 1925
Size: 162 5428
BssSI C'ACGA_G
Cuts at: 2315 3650 4662 2315
Size: 1335 1012 3243
Fragments arranged by size:
3243 1335 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5590
BstBI TT'CG_AA
Cuts at: 4394 4394
Size: 5590
BstEII G'GTnAC_C
Cuts at: 1184 2659 1184
Size: 1475 4115
BstXI CCAn_nnnn'nTGG
Cuts at: 2227 2227
Size: 5590
BstYI r'GATC_y
Cuts at: 1972 2920 3287 3672 3689 5141 5227 5239 5585
Size: 948 367 385 17 1452 86 12 346
Cuts at: 5585 1972
Size: 1977
Fragments arranged by size:
1977 1452 948 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4099
Fragments arranged by size:
4099 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2519 487
Size: 683 56 1293 3558
Fragments arranged by size:
3558 1293 683 56
BtsI GCAGTG_nn'
Cuts at: 3106 3900 3920 3106
Size: 794 20 4776
Fragments arranged by size:
4776 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5590
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5555
DraI TTT'AAA
Cuts at: 2894 2894
Size: 5590
DraIII CAC_nnn'GTG
Cuts at: 2659 2659
Size: 5590
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2336 4597 809
Size: 507 1020 2261 1802
Fragments arranged by size:
2261 1802 1020 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2695 3037
Size: 54 1425 50 275 18 216 649 342
Cuts at: 3037 3926 5555 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 649 342 275 216 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2046 3037 1537
Size: 275 234 991 4090
Fragments arranged by size:
4090 991 275 234
EarI CTCTTCn'nnn_
Cuts at: 1353 2229 3520 1353
Size: 876 1291 3423
Fragments arranged by size:
3423 1291 876
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4177 4551 4697 387
Size: 1064 138 2588 374 146 1280
Fragments arranged by size:
2588 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2227 2300 3653 4250 5037 5305 1239
Size: 393 595 73 1353 597 787 268 1524
Fragments arranged by size:
1524 1353 787 597 595 393 268 73
EcoO109I rG'GnC_Cy
Cuts at: 2515 2515
Size: 5590
EcoRI G'AATT_C
Cuts at: 2899 3277 2899
Size: 378 5212
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2620 2620
Size: 5590
FspI TGC'GCA
Cuts at: 4096 4096
Size: 5590
HaeII r_GCGC'y
Cuts at: 1678 2302 2483 4737 1678
Size: 624 181 2254 2531
Fragments arranged by size:
2531 2254 624 181
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4231 4263 4305 4337 1373
Size: 32 135 32 2659 32 42 32 2626
Fragments arranged by size:
2659 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2934 3180 669
Size: 988 281 996 246 3079
Fragments arranged by size:
3079 996 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5590
HpaI GTT'AAC
Cuts at: 3180 3180
Size: 5590
KpnI G_GTAC'C
Cuts at: 2915 2915
Size: 5590
MfeI C'AATT_G
Cuts at: 3189 3189
Size: 5590
MluI A'CGCG_T
Cuts at: 5466 5466
Size: 5590
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4704 4888 1305
Size: 3399 184 2007
Fragments arranged by size:
3399 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2697 10
Size: 54 1425 343 865 2903
Fragments arranged by size:
2903 1425 865 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2210 2870 3548 3907 4066 518
Size: 648 1044 660 678 359 159 2042
Fragments arranged by size:
2042 1044 678 660 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2101 2182 3006 3683 4831 5076 1606
Size: 495 81 824 677 1148 245 2120
Fragments arranged by size:
2120 1148 824 677 495 245 81
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4099
Fragments arranged by size:
4099 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5590
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5590
NotI GC'GGCC_GC
Cuts at: 3037 3037
Size: 5590
NspI r_CATG'y
Cuts at: 1132 1229 2948 4493 1132
Size: 97 1719 1545 2229
Fragments arranged by size:
2229 1719 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2035 2576 1348
Size: 687 541 4362
Fragments arranged by size:
4362 687 541
PciI A'CATG_T
Cuts at: 4489 4489
Size: 5590
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5590
PfoI T'CCnGG_A
Cuts at: 2058 2058
Size: 5590
PmeI GTTT'AAAC
Cuts at: 2894 2894
Size: 5590
PmlI CAC'GTG
Cuts at: 2872 2872
Size: 5590
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3659 3691 5172 5204 3659
Size: 32 1481 32 4045
Fragments arranged by size:
4045 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5590
PsiI TTA'TAA
Cuts at: 3160 3160
Size: 5590
PstI C_TGCA'G
Cuts at: 830 2942 830
Size: 2112 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5590
PvuII CAG'CTG
Cuts at: 2101 2101
Size: 5590
SalI G'TCGA_C
Cuts at: 1936 2932 1936
Size: 996 4594
SbfI CC_TGCA'GG
Cuts at: 2942 2942
Size: 5590
ScaI AGT'ACT
Cuts at: 3838 5550 3838
Size: 1712 3878
SfcI C'TryA_G
Cuts at: 826 1045 2938 4073 4754 4945 826
Size: 219 1893 1135 681 191 1471
Fragments arranged by size:
1893 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5590
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5590
SmaI CCC'GGG
Cuts at: 1872 2917 1872
Size: 1045 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2291 2716 2876 3694 4595 4857
Size: 197 926 348 425 160 818 901 262
Cuts at: 4857 820
Size: 1553
Fragments arranged by size:
1553 926 901 818 425 348 262 197
160
SnaBI TAC'GTA
Cuts at: 493 3381 493
Size: 2888 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5590
SphI G_CATG'C
Cuts at: 1132 2948 1132
Size: 1816 3774
SspI AAT'ATT
Cuts at: 5 52 3514 5
Size: 47 3462 2081
Fragments arranged by size:
3462 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5590
StyI C'CwwG_G
Cuts at: 513 1065 2004 2419 2610 3029 513
Size: 552 939 415 191 419 3074
Fragments arranged by size:
3074 939 552 419 415 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3617 3634 3787 3972 4417 325
Size: 1255 2037 17 153 185 445 1498
Fragments arranged by size:
2037 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2136 2558 3836
Size: 274 80 33 51 1443 159 422 1278
Cuts at: 3836 5548 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1278 422 274 159 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5550 5582 5550
Size: 32 5558
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5590
XbaI T'CTAG_A
Cuts at: 2926 2926
Size: 5590
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2227 2227
Size: 5590
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5590
XmaI C'CCGG_G
Cuts at: 1870 2915 1870
Size: 1045 4545
XmnI GAAnn'nnTTC
Cuts at: 3719 3719
Size: 5590
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2617 3398 276
Size: 53 83 186 936 1083 781 2468
Fragments arranged by size:
2468 1083 936 781 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI