Restriction map for
pFN21AE1434
(Circular) (Six-base) MAPSORT of: pFN21AE1434.seq Check: 9043 from: 1 to: 7057
pFN21AE1434 7057 bp
With 182 enzymes: SgfI *
February 20, 2021 22:13 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4867 278
Size: 53 83 186 936 3331 2468
Fragments arranged by size:
3331 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4400 1937
Size: 2463 4594
Acc65I G'GTAC_C
Cuts at: 4378 4378
Size: 7057
AclI AA'CG_TT
Cuts at: 1563 2936 5184 5557 1563
Size: 1373 2248 373 3063
Fragments arranged by size:
3063 2248 1373 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2538 3599 5120 6504 6772 1632
Size: 906 1061 1521 1384 268 1917
Fragments arranged by size:
1917 1521 1384 1061 906 268
AfeI AGC'GCT
Cuts at: 4247 4247
Size: 7057
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6860
AflIII A'CryG_T
Cuts at: 1203 5956 6933 1203
Size: 4753 977 1327
Fragments arranged by size:
4753 1327 977
AgeI A'CCGG_T
Cuts at: 5888 5888
Size: 7057
AhdI GACnn_n'nnGTC
Cuts at: 5786 5786
Size: 7057
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7057
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7057
AlwNI CAG_nnn'CTG
Cuts at: 2162 2245 3991 4800 6372 2162
Size: 83 1746 809 1572 2847
Fragments arranged by size:
2847 1746 1572 809 83
ApaLI G'TGCA_C
Cuts at: 5114 6270 5114
Size: 1156 5901
ApoI r'AATT_y
Cuts at: 1493 2746 3112 3139 3384 3427 3623 4366 4592
Size: 1253 366 27 245 43 196 743 226
Cuts at: 4592 4744 6853 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1253 743 366 245 226 196
152 43 27
AseI AT'TA_AT
Cuts at: 160 1032 5611 160
Size: 872 4579 1606
Fragments arranged by size:
4579 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7057
AvaI C'yCGr_G
Cuts at: 1870 1943 4382 1870
Size: 73 2439 4545
Fragments arranged by size:
4545 2439 73
AvrII C'CTAG_G
Cuts at: 2419 2419
Size: 7057
BamHI G'GATC_C
Cuts at: 4387 4754 4387
Size: 367 6690
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4378 5833 618
Size: 325 217 627 2591 1455 1842
Fragments arranged by size:
2591 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2154 4221 4376 1391
Size: 763 2067 155 4072
Fragments arranged by size:
4072 2067 763 155
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2346 3587 4016 928
Size: 971 447 1241 429 3969
Fragments arranged by size:
3969 1241 971 447 429
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5248 5282 1903
Size: 34 50 34 3227 34 3678
Fragments arranged by size:
3678 3227 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4949 6159 4949
Size: 1210 5847
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7057
BfrBI ATG'CAT
Cuts at: 3932 3932
Size: 7057
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5668 136
Size: 107 122 71 631 605 3996 1525
Fragments arranged by size:
3996 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2557 7052 2557
Size: 4495 2562
BlpI GC'TnA_GC
Cuts at: 4311 4474 4311
Size: 163 6894
Bme1580I G_kGCm'C
Cuts at: 1790 5118 6274 1790
Size: 3328 1156 2573
Fragments arranged by size:
3328 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7057
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2408 3973 4788
Size: 416 53 83 399 24 991 1565 815
Cuts at: 4788 5746 442
Size: 958 1753
Fragments arranged by size:
1753 1565 991 958 815 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7057
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2222 2666 3863 4013 4235 4310 5717 1239
Size: 983 444 1197 150 222 75 1407 2579
Fragments arranged by size:
2579 1407 1197 983 444 222 150 75
Bpu10I CC'TnA_GC
Cuts at: 2122 2122
Size: 7057
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2552 2561 5182 6047 6345 2552
Size: 9 2621 865 298 3264
Fragments arranged by size:
3264 2621 865 298 9
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5720 882
Size: 617 4221 2219
Fragments arranged by size:
4221 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4848 493
Size: 711 3644 2702
Fragments arranged by size:
3644 2702 711
BsaBI GATnn'nnATC
Cuts at: 7051 7051
Size: 7057
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4864 5246 5881 275
Size: 53 83 186 936 3331 382 635 1451
Fragments arranged by size:
3331 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2946 3218 5490 5888 6162 6309 6720 2946
Size: 272 2272 398 274 147 411 3283
Fragments arranged by size:
3283 2272 411 398 274 272 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6832
Fragments arranged by size:
6832 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7057
BseYI C'CCAG_C
Cuts at: 3908 4242 6260 6756 3908
Size: 334 2018 496 4209
Fragments arranged by size:
4209 2018 496 334
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6484
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4507 5268 6296 1540
Size: 275 186 2506 761 1028 2301
Fragments arranged by size:
2506 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2993 3796 5118 5203 6274 2993
Size: 803 1322 85 1071 3776
Fragments arranged by size:
3776 1322 1071 803 85
BsmI GAATG_Cn'
Cuts at: 3366 3535 4568 4661 3366
Size: 169 1033 93 5762
Fragments arranged by size:
5762 1033 169 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2154 2993 3796 4221 4376 5118 5203
Size: 399 364 839 803 425 155 742 85
Cuts at: 5203 6274 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 839 803 742 425 399 364
155 85
BspEI T'CCGG_A
Cuts at: 2946 6720 2946
Size: 3774 3283
BspHI T'CATG_A
Cuts at: 4946 6676 4946
Size: 1730 5327
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3715 4412 844
Size: 2871 697 3489
Fragments arranged by size:
3489 2871 697
BsrBI CCG'CTC
Cuts at: 1394 1823 3952 4944 1394
Size: 429 2129 992 3507
Fragments arranged by size:
3507 2129 992 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2107 5552 5726 65
Size: 1183 859 3445 174 1396
Fragments arranged by size:
3445 1396 1183 859 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5701 5888 1700
Size: 110 3891 187 2869
Fragments arranged by size:
3891 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7057
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7057
BssSI C'ACGA_G
Cuts at: 5117 6129 5117
Size: 1012 6045
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7057
BstBI TT'CG_AA
Cuts at: 5861 5861
Size: 7057
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7057
BstXI CCAn_nnnn'nTGG
Cuts at: 3379 3716 3379
Size: 337 6720
BstYI r'GATC_y
Cuts at: 1972 2126 2557 2917 3100 4387 4754 5139 5156
Size: 154 431 360 183 1287 367 385 17
Cuts at: 5156 6608 6694 6706 7052 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1287 431 385 367 360 346
183 154 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2146 2146
Size: 7057
BtgI C'CryG_G
Cuts at: 513 1065 2673 3841 4299 513
Size: 552 1608 1168 458 3271
Fragments arranged by size:
3271 1608 1168 552 458
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6318
Fragments arranged by size:
6318 683 56
BtsI GCAGTG_nn'
Cuts at: 4573 5367 5387 4573
Size: 794 20 6243
Fragments arranged by size:
6243 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7057
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 1998 2033 2655 2690 529
Size: 35 1434 35 622 35 4896
Fragments arranged by size:
4896 1434 622 35 35 35
DraI TTT'AAA
Cuts at: 2133 3081 4361 2133
Size: 948 1280 4829
Fragments arranged by size:
4829 1280 948
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6064 809
Size: 507 4748 1802
Fragments arranged by size:
4748 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3305 3657 3677
Size: 54 1425 50 275 18 1475 352 20
Cuts at: 3677 3718 4504 5393 7022 8
Size: 41 786 889 1629 43
Fragments arranged by size:
1629 1475 1425 889 786 352 275 54
50 43 41 20 18
EagI C'GGCC_G
Cuts at: 1537 1812 4504 1537
Size: 275 2692 4090
Fragments arranged by size:
4090 2692 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4124 4987 1353
Size: 2771 863 3423
Fragments arranged by size:
3423 2771 863
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3728 5644 6018 6164 387
Size: 1064 138 2139 1916 374 146 1280
Fragments arranged by size:
2139 1916 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2222 2538 2666 3599 3863 4013 4235
Size: 393 590 316 128 933 264 150 222
Cuts at: 4235 4310 5120 5717 6504 6772 1239
Size: 75 810 597 787 268 1524
Fragments arranged by size:
1524 933 810 787 597 590 393 316
268 264 222 150 128 75
EcoNI CCTnn'n_nnAGG
Cuts at: 2057 2057
Size: 7057
EcoO109I rG'GnC_Cy
Cuts at: 3823 3823
Size: 7057
EcoRI G'AATT_C
Cuts at: 3623 4366 4744 3623
Size: 743 378 5936
Fragments arranged by size:
5936 743 378
EcoRV GAT'ATC
Cuts at: 3262 3262
Size: 7057
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4054 4054
Size: 7057
FspI TGC'GCA
Cuts at: 5563 5563
Size: 7057
HaeII r_GCGC'y
Cuts at: 1678 4249 6204 1678
Size: 2571 1955 2531
Fragments arranged by size:
2571 2531 1955
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3689 3721 5698 5730 5772
Size: 32 135 32 2117 32 1977 32 42
Cuts at: 5772 5804 1373
Size: 32 2626
Fragments arranged by size:
2626 2117 1977 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2940 4401 4647 669
Size: 988 281 1002 1461 246 3079
Fragments arranged by size:
3079 1461 1002 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7057
HpaI GTT'AAC
Cuts at: 2940 4647 2940
Size: 1707 5350
KpnI G_GTAC'C
Cuts at: 4382 4382
Size: 7057
MfeI C'AATT_G
Cuts at: 4656 4656
Size: 7057
MluI A'CGCG_T
Cuts at: 6933 6933
Size: 7057
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2208 3732 6171 6355 1305
Size: 903 1524 2439 184 2007
Fragments arranged by size:
2439 2007 1524 903 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3307 3659 3679 3720 10
Size: 54 1425 343 1475 352 20 41 3347
Fragments arranged by size:
3347 1475 1425 352 343 54 41 20
MslI CAynn'nnrTG
Cuts at: 518 1166 3714 5015 5374 5533 518
Size: 648 2548 1301 359 159 2042
Fragments arranged by size:
2548 2042 1301 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4315 4473 5150 6298 6543 1606
Size: 2709 158 677 1148 245 2120
Fragments arranged by size:
2709 2120 1148 677 245 158
NcoI C'CATG_G
Cuts at: 513 1065 3841 4299 513
Size: 552 2776 458 3271
Fragments arranged by size:
3271 2776 552 458
NdeI CA'TA_TG
Cuts at: 387 2622 387
Size: 2235 4822
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7057
NotI GC'GGCC_GC
Cuts at: 4504 4504
Size: 7057
NsiI A_TGCA'T
Cuts at: 3934 3934
Size: 7057
NspI r_CATG'y
Cuts at: 1132 1229 3787 4415 5960 1132
Size: 97 2558 628 1545 2229
Fragments arranged by size:
2558 2229 1545 628 97
PasI CC'CwG_GG
Cuts at: 1348 2299 1348
Size: 951 6106
PciI A'CATG_T
Cuts at: 5956 5956
Size: 7057
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2245 3991 1108
Size: 1137 1746 4174
Fragments arranged by size:
4174 1746 1137
PfoI T'CCnGG_A
Cuts at: 3984 3984
Size: 7057
PmeI GTTT'AAAC
Cuts at: 4361 4361
Size: 7057
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5126 5158 6639 6671 5126
Size: 32 1481 32 5512
Fragments arranged by size:
5512 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3823 3823
Size: 7057
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7057
PsiI TTA'TAA
Cuts at: 4627 4627
Size: 7057
PstI C_TGCA'G
Cuts at: 830 4409 830
Size: 3579 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7057
SalI G'TCGA_C
Cuts at: 1936 4399 1936
Size: 2463 4594
SbfI CC_TGCA'GG
Cuts at: 4409 4409
Size: 7057
ScaI AGT'ACT
Cuts at: 5305 7017 5305
Size: 1712 5345
SfcI C'TryA_G
Cuts at: 826 1045 2219 2561 4405 5540 6221 6412 826
Size: 219 1174 342 1844 1135 681 191 1471
Fragments arranged by size:
1844 1471 1174 1135 681 342 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7057
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7057
SmaI CCC'GGG
Cuts at: 1872 4384 1872
Size: 2512 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2531 2540 5161 6062 6324 820
Size: 197 926 588 9 2621 901 262 1553
Fragments arranged by size:
2621 1553 926 901 588 262 197 9
SnaBI TAC'GTA
Cuts at: 493 4848 493
Size: 4355 2702
SpeI A'CTAG_T
Cuts at: 152 3508 152
Size: 3356 3701
SphI G_CATG'C
Cuts at: 1132 4415 1132
Size: 3283 3774
SspI AAT'ATT
Cuts at: 5 52 3323 4981 5
Size: 47 3271 1658 2081
Fragments arranged by size:
3271 2081 1658 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7057
StyI C'CwwG_G
Cuts at: 513 1065 2058 2419 2963 3841 4299 4496 513
Size: 552 993 361 544 878 458 197 3074
Fragments arranged by size:
3074 993 878 552 544 458 361 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3955 5084 5101 5254 5439 5884 325
Size: 1255 2375 1129 17 153 185 445 1498
Fragments arranged by size:
2375 1498 1255 1129 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5303 7015 96
Size: 274 80 33 51 1443 3326 1712 138
Fragments arranged by size:
3326 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7017 7049 7017
Size: 32 7025
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7057
XbaI T'CTAG_A
Cuts at: 4393 4393
Size: 7057
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2016 3839 4285 2016
Size: 1823 446 4788
Fragments arranged by size:
4788 1823 446
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7057
XmaI C'CCGG_G
Cuts at: 1870 4382 1870
Size: 2512 4545
XmnI GAAnn'nnTTC
Cuts at: 5186 5186
Size: 7057
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4865 276
Size: 53 83 186 936 3331 2468
Fragments arranged by size:
3331 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BbeI BbvCI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI