Restriction map for
pFN21AE1433
(Circular) (Six-base) MAPSORT of: pFN21AE1433.seq Check: 423 from: 1 to: 6202
pFN21AE1433 6202 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4012 278
Size: 53 83 186 936 2476 2468
Fragments arranged by size:
2476 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3545 1937
Size: 1608 4594
Acc65I G'GTAC_C
Cuts at: 3523 3523
Size: 6202
AclI AA'CG_TT
Cuts at: 1563 4329 4702 1563
Size: 2766 373 3063
Fragments arranged by size:
3063 2766 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2030 4265 5649 5917 1632
Size: 398 2235 1384 268 1917
Fragments arranged by size:
2235 1917 1384 398 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6005
AflIII A'CryG_T
Cuts at: 1203 5101 6078 1203
Size: 3898 977 1327
Fragments arranged by size:
3898 1327 977
AgeI A'CCGG_T
Cuts at: 5033 5033
Size: 6202
AhdI GACnn_n'nnGTC
Cuts at: 4931 4931
Size: 6202
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6202
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6202
AlwNI CAG_nnn'CTG
Cuts at: 3945 5517 3945
Size: 1572 4630
ApaI G_GGCC'C
Cuts at: 2959 2959
Size: 6202
ApaLI G'TGCA_C
Cuts at: 4259 5415 4259
Size: 1156 5046
ApoI r'AATT_y
Cuts at: 1493 3511 3737 3889 5998 1493
Size: 2018 226 152 2109 1697
Fragments arranged by size:
2109 2018 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4756 160
Size: 872 3724 1606
Fragments arranged by size:
3724 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6202
AvaI C'yCGr_G
Cuts at: 1870 1943 2811 3336 3527 1870
Size: 73 868 525 191 4545
Fragments arranged by size:
4545 868 525 191 73
BamHI G'GATC_C
Cuts at: 3532 3899 3532
Size: 367 5835
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2735 3131 3523 4978 618
Size: 325 217 627 948 396 392 1455 1842
Fragments arranged by size:
1842 1455 948 627 396 392 325 217
BanII G_rGCy'C
Cuts at: 1391 2822 2959 3246 3362 3521 1391
Size: 1431 137 287 116 159 4072
Fragments arranged by size:
4072 1431 287 159 137 116
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2498 928
Size: 971 599 4632
Fragments arranged by size:
4632 971 599
BbvCI CC'TCA_GC
Cuts at: 2748 2748
Size: 6202
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4393 4427 1903
Size: 34 2456 34 3678
Fragments arranged by size:
3678 2456 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2537 4094 5304 2537
Size: 1557 1210 3435
Fragments arranged by size:
3435 1557 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6202
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3311 3377 4813
Size: 107 122 71 631 605 1639 66 1436
Cuts at: 4813 136
Size: 1525
Fragments arranged by size:
1639 1525 1436 631 605 122 107 71
66
BglII A'GATC_T
Cuts at: 6197 6197
Size: 6202
BlpI GC'TnA_GC
Cuts at: 3619 3619
Size: 6202
Bme1580I G_kGCm'C
Cuts at: 1790 2738 2959 3134 4263 5419 1790
Size: 948 221 175 1129 1156 2573
Fragments arranged by size:
2573 1156 1129 948 221 175
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6202
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3933 4891 442
Size: 416 53 83 399 24 2516 958 1753
Fragments arranged by size:
2516 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6202
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3265 3265
Size: 6202
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2467 3045 4862 1239
Size: 1228 578 1817 2579
Fragments arranged by size:
2579 1817 1228 578
Bpu10I CC'TnA_GC
Cuts at: 2288 2748 2825 2288
Size: 460 77 5665
Fragments arranged by size:
5665 460 77
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4327 5192 5490 4327
Size: 865 298 5039
Fragments arranged by size:
5039 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3231 3392 4865 882
Size: 617 1732 161 1473 2219
Fragments arranged by size:
2219 1732 1473 617 161
BsaAI yAC'GTr
Cuts at: 493 1204 3993 493
Size: 711 2789 2702
Fragments arranged by size:
2789 2702 711
BsaBI GATnn'nnATC
Cuts at: 6196 6196
Size: 6202
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2321 4009 4391 5026
Size: 53 83 186 936 788 1688 382 635
Cuts at: 5026 275
Size: 1451
Fragments arranged by size:
1688 1451 936 788 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4635 5033 5307 5454 5865 4635
Size: 398 274 147 411 4972
Fragments arranged by size:
4972 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5977
Fragments arranged by size:
5977 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6202
BseYI C'CCAG_C
Cuts at: 2952 3036 3060 5405 5901 2952
Size: 84 24 2345 496 3253
Fragments arranged by size:
3253 2345 496 84 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2018 2774 2789 3486 1163
Size: 573 282 756 15 697 3879
Fragments arranged by size:
3879 756 697 573 282 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3652 4413 5441 1540
Size: 275 186 1651 761 1028 2301
Fragments arranged by size:
2301 1651 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3022 3170 3215 3246 4263 4348 5419 3022
Size: 148 45 31 1017 85 1071 3805
Fragments arranged by size:
3805 1071 1017 148 85 45 31
BsmI GAATG_Cn'
Cuts at: 3713 3806 3713
Size: 93 6109
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2738 2822 2959 3022 3134 3170 3215
Size: 399 948 84 137 63 112 36 45
Cuts at: 3215 3246 3362 3521 4263 4348 5419 1391
Size: 31 116 159 742 85 1071 2174
Fragments arranged by size:
2174 1071 948 742 399 159 137 116
112 85 84 63 45 36 31
BspEI T'CCGG_A
Cuts at: 5865 5865
Size: 6202
BspHI T'CATG_A
Cuts at: 4091 5821 4091
Size: 1730 4472
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2040 2504 3557 844
Size: 1196 464 1053 3489
Fragments arranged by size:
3489 1196 1053 464
BsrBI CCG'CTC
Cuts at: 1394 1823 3451 4089 1394
Size: 429 1628 638 3507
Fragments arranged by size:
3507 1628 638 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4697 4871 65
Size: 1183 3449 174 1396
Fragments arranged by size:
3449 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2159 4846 5033 1700
Size: 110 349 2687 187 2869
Fragments arranged by size:
2869 2687 349 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6202
BssHII G'CGCG_C
Cuts at: 1925 2081 1925
Size: 156 6046
BssSI C'ACGA_G
Cuts at: 4262 5274 4262
Size: 1012 5190
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2041 2078 2662 794
Size: 1247 37 584 4334
Fragments arranged by size:
4334 1247 584 37
BstBI TT'CG_AA
Cuts at: 2788 5006 2788
Size: 2218 3984
BstEII G'GTnAC_C
Cuts at: 1184 2231 1184
Size: 1047 5155
BstXI CCAn_nnnn'nTGG
Cuts at: 3115 3115
Size: 6202
BstYI r'GATC_y
Cuts at: 1972 2391 3532 3899 4284 4301 5753 5839 5851
Size: 419 1141 367 385 17 1452 86 12
Cuts at: 5851 6197 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1141 419 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3404 3404
Size: 6202
BtgI C'CryG_G
Cuts at: 513 1065 2004 2601 3189 3256 3300 513
Size: 552 939 597 588 67 44 3415
Fragments arranged by size:
3415 939 597 588 552 67 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2234 487
Size: 683 56 1008 4455
Fragments arranged by size:
4455 1008 683 56
BtsI GCAGTG_nn'
Cuts at: 2260 2749 3718 4512 4532 2260
Size: 489 969 794 20 3930
Fragments arranged by size:
3930 969 794 489 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6202
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6167
DraI TTT'AAA
Cuts at: 3506 3506
Size: 6202
DraIII CAC_nnn'GTG
Cuts at: 2908 2921 2908
Size: 13 6189
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5209 809
Size: 507 3893 1802
Fragments arranged by size:
3893 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2274 2598 2866
Size: 54 1425 50 275 18 444 324 268
Cuts at: 2866 2910 2923 2976 3303 3649 4538 6167 8
Size: 44 13 53 327 346 889 1629 43
Fragments arranged by size:
1629 1425 889 444 346 327 324 275
268 54 53 50 44 43 18 13
EagI C'GGCC_G
Cuts at: 1537 1812 3649 1537
Size: 275 1837 4090
Fragments arranged by size:
4090 1837 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4132 1353
Size: 2779 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4789 5163 5309 387
Size: 1064 138 3200 374 146 1280
Fragments arranged by size:
3200 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3244 3244
Size: 6202
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2030 2467 3045 4265 4862 5649 5917
Size: 393 398 437 578 1220 597 787 268
Cuts at: 5917 1239
Size: 1524
Fragments arranged by size:
1524 1220 787 597 578 437 398 393
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2291 2291
Size: 6202
EcoO109I rG'GnC_Cy
Cuts at: 2956 3174 3340 2956
Size: 218 166 5818
Fragments arranged by size:
5818 218 166
EcoRI G'AATT_C
Cuts at: 3511 3889 3511
Size: 378 5824
FspI TGC'GCA
Cuts at: 4708 4708
Size: 6202
HaeII r_GCGC'y
Cuts at: 1678 2160 3418 5349 1678
Size: 482 1258 1931 2531
Fragments arranged by size:
2531 1931 1258 482
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2021 2053 4843 4875 4917
Size: 32 135 32 449 32 2790 32 42
Cuts at: 4917 4949 1373
Size: 32 2626
Fragments arranged by size:
2790 2626 449 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3546 3792 669
Size: 988 281 1608 246 3079
Fragments arranged by size:
3079 1608 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6202
HpaI GTT'AAC
Cuts at: 3792 3792
Size: 6202
KpnI G_GTAC'C
Cuts at: 3527 3527
Size: 6202
MfeI C'AATT_G
Cuts at: 3801 3801
Size: 6202
MluI A'CGCG_T
Cuts at: 6078 6078
Size: 6202
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3408 5316 5500 1305
Size: 2103 1908 184 2007
Fragments arranged by size:
2103 2007 1908 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2276 2868 2912 2925 2978
Size: 54 1425 343 444 592 44 13 53
Cuts at: 2978 3305 10
Size: 327 2907
Fragments arranged by size:
2907 1425 592 444 343 327 54 53
44 13
MslI CAynn'nnrTG
Cuts at: 518 1166 2253 2880 2883 4160 4519 4678 518
Size: 648 1087 627 3 1277 359 159 2042
Fragments arranged by size:
2042 1277 1087 648 627 359 159 3
MspA1I CmG'CkG
Cuts at: 1606 2050 3618 4295 5443 5688 1606
Size: 444 1568 677 1148 245 2120
Fragments arranged by size:
2120 1568 1148 677 444 245
NaeI GCC'GGC
Cuts at: 2161 2161
Size: 6202
NcoI C'CATG_G
Cuts at: 513 1065 2004 3256 3300 513
Size: 552 939 1252 44 3415
Fragments arranged by size:
3415 1252 939 552 44
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6202
NgoMIV G'CCGG_C
Cuts at: 2159 2159
Size: 6202
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6202
NotI GC'GGCC_GC
Cuts at: 3649 3649
Size: 6202
NspI r_CATG'y
Cuts at: 1132 1229 3560 5105 1132
Size: 97 2331 1545 2229
Fragments arranged by size:
2331 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6202
PciI A'CATG_T
Cuts at: 5101 5101
Size: 6202
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3222 1108
Size: 2114 4088
PfoI T'CCnGG_A
Cuts at: 2529 3022 2529
Size: 493 5709
PmeI GTTT'AAAC
Cuts at: 3506 3506
Size: 6202
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4271 4303 5784 5816 4271
Size: 32 1481 32 4657
Fragments arranged by size:
4657 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3340 3340
Size: 6202
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6202
PsiI TTA'TAA
Cuts at: 3772 3772
Size: 6202
PspOMI G'GGCC_C
Cuts at: 2955 2955
Size: 6202
PstI C_TGCA'G
Cuts at: 830 2658 3051 3554 830
Size: 1828 393 503 3478
Fragments arranged by size:
3478 1828 503 393
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6202
PvuII CAG'CTG
Cuts at: 2050 2050
Size: 6202
SacI G_AGCT'C
Cuts at: 3246 3246
Size: 6202
SalI G'TCGA_C
Cuts at: 1936 3544 1936
Size: 1608 4594
SbfI CC_TGCA'GG
Cuts at: 3554 3554
Size: 6202
ScaI AGT'ACT
Cuts at: 2454 4450 6162 2454
Size: 1996 1712 2494
Fragments arranged by size:
2494 1996 1712
SfcI C'TryA_G
Cuts at: 826 1045 2458 2654 3047 3550 4685 5366 5557
Size: 219 1413 196 393 503 1135 681 191
Cuts at: 5557 826
Size: 1471
Fragments arranged by size:
1471 1413 1135 681 503 393 219 196
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6202
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6202
SmaI CCC'GGG
Cuts at: 1872 3338 3529 1872
Size: 1466 191 4545
Fragments arranged by size:
4545 1466 191
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4306 5207 5469 820
Size: 197 926 2363 901 262 1553
Fragments arranged by size:
2363 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3993 493
Size: 3500 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6202
SphI G_CATG'C
Cuts at: 1132 3560 1132
Size: 2428 3774
SspI AAT'ATT
Cuts at: 5 52 4126 5
Size: 47 4074 2081
Fragments arranged by size:
4074 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6202
StyI C'CwwG_G
Cuts at: 513 1065 2004 2100 2199 3256 3300 3306 3490
Size: 552 939 96 99 1057 44 6 184
Cuts at: 3490 3641 513
Size: 151 3074
Fragments arranged by size:
3074 1057 939 552 184 151 99 96
44 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2074 2143 3074 4229 4246 4399 4584
Size: 1255 494 69 931 1155 17 153 185
Cuts at: 4584 5029 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1155 931 494 445 185 153
69 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2452 2593 4448
Size: 274 80 33 51 1443 475 141 1855
Cuts at: 4448 6160 96
Size: 1712 138
Fragments arranged by size:
1855 1712 1443 475 274 141 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6162 6194 6162
Size: 32 6170
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6202
XbaI T'CTAG_A
Cuts at: 3538 3538
Size: 6202
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2939 3307 2939
Size: 368 5834
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6202
XmaI C'CCGG_G
Cuts at: 1870 3336 3527 1870
Size: 1466 191 4545
Fragments arranged by size:
4545 1466 191
XmnI GAAnn'nnTTC
Cuts at: 4331 4331
Size: 6202
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4010 276
Size: 53 83 186 936 2476 2468
Fragments arranged by size:
2476 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI