Restriction map for
pFN21AE1430
(Circular) (Six-base) MAPSORT of: pFN21AE1430.seq Check: 4116 from: 1 to: 5551
pFN21AE1430 5551 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3361 278
Size: 53 83 186 936 1825 2468
Fragments arranged by size:
2468 1825 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2894 1937
Size: 957 4594
Acc65I G'GTAC_C
Cuts at: 2872 2872
Size: 5551
AclI AA'CG_TT
Cuts at: 1563 3678 4051 1563
Size: 2115 373 3063
Fragments arranged by size:
3063 2115 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3614 4998 5266 1632
Size: 1982 1384 268 1917
Fragments arranged by size:
1982 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5354
AflIII A'CryG_T
Cuts at: 1203 4450 5427 1203
Size: 3247 977 1327
Fragments arranged by size:
3247 1327 977
AgeI A'CCGG_T
Cuts at: 4382 4382
Size: 5551
AhdI GACnn_n'nnGTC
Cuts at: 4280 4280
Size: 5551
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5551
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5551
AlwNI CAG_nnn'CTG
Cuts at: 2098 3294 4866 2098
Size: 1196 1572 2783
Fragments arranged by size:
2783 1572 1196
ApaLI G'TGCA_C
Cuts at: 2119 3608 4764 2119
Size: 1489 1156 2906
Fragments arranged by size:
2906 1489 1156
ApoI r'AATT_y
Cuts at: 1493 2289 2860 3086 3238 5347 1493
Size: 796 571 226 152 2109 1697
Fragments arranged by size:
2109 1697 796 571 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4105 160
Size: 872 3073 1606
Fragments arranged by size:
3073 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5551
AvaI C'yCGr_G
Cuts at: 1870 1943 2876 1870
Size: 73 933 4545
Fragments arranged by size:
4545 933 73
BamHI G'GATC_C
Cuts at: 2881 3248 2881
Size: 367 5184
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2107 2872 4327 618
Size: 325 217 627 320 765 1455 1842
Fragments arranged by size:
1842 1455 765 627 325 320 217
BanII G_rGCy'C
Cuts at: 1391 2045 2781 2870 1391
Size: 654 736 89 4072
Fragments arranged by size:
4072 736 654 89
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2598 2829 928
Size: 971 699 231 3650
Fragments arranged by size:
3650 971 699 231
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2347 2381 3742 3776 1903
Size: 34 50 34 326 34 1361 34 3678
Fragments arranged by size:
3678 1361 326 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2659 3443 4653 2659
Size: 784 1210 3557
Fragments arranged by size:
3557 1210 784
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5551
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2089 2134 4162
Size: 107 122 71 631 605 417 45 2028
Cuts at: 4162 136
Size: 1525
Fragments arranged by size:
2028 1525 631 605 417 122 107 71
45
BglII A'GATC_T
Cuts at: 5546 5546
Size: 5551
BlpI GC'TnA_GC
Cuts at: 2141 2780 2968 2141
Size: 639 188 4724
Fragments arranged by size:
4724 639 188
Bme1580I G_kGCm'C
Cuts at: 1790 2031 2123 3612 4768 1790
Size: 241 92 1489 1156 2573
Fragments arranged by size:
2573 1489 1156 241 92
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5551
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2710 3282 4240
Size: 416 53 83 399 24 1293 572 958
Cuts at: 4240 442
Size: 1753
Fragments arranged by size:
1753 1293 958 572 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5551
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2467 2467
Size: 5551
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2343 2813 4211 1239
Size: 1104 470 1398 2579
Fragments arranged by size:
2579 1398 1104 470
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2813 3676 4541 4839 2813
Size: 863 865 298 3525
Fragments arranged by size:
3525 865 863 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4214 882
Size: 617 2715 2219
Fragments arranged by size:
2715 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3342 493
Size: 711 2138 2702
Fragments arranged by size:
2702 2138 711
BsaBI GATnn'nnATC
Cuts at: 5545 5545
Size: 5551
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3358 3740 4375 275
Size: 53 83 186 936 1825 382 635 1451
Fragments arranged by size:
1825 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3984 4382 4656 4803 5214 3984
Size: 398 274 147 411 4321
Fragments arranged by size:
4321 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2482 2512 1178
Size: 30 165 30 1079 30 4217
Fragments arranged by size:
4217 1079 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2035 2456 1186
Size: 849 421 4281
Fragments arranged by size:
4281 849 421
BseYI C'CCAG_C
Cuts at: 2038 4754 5250 2038
Size: 2716 496 2339
Fragments arranged by size:
2716 2339 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2210 1163
Size: 573 474 4504
Fragments arranged by size:
4504 573 474
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2171 2740 3001 3762 4790 1540
Size: 275 186 170 569 261 761 1028 2301
Fragments arranged by size:
2301 1028 761 569 275 261 186 170
BsiHKAI G_wGCw'C
Cuts at: 2123 2781 2799 3612 3697 4768 2123
Size: 658 18 813 85 1071 2906
Fragments arranged by size:
2906 1071 813 658 85 18
BsmI GAATG_Cn'
Cuts at: 3062 3155 3062
Size: 93 5458
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2045 2123 2781 2799 2870 3612
Size: 399 241 14 78 658 18 71 742
Cuts at: 3612 3697 4768 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 658 399 241 85 78
71 18 14
BspEI T'CCGG_A
Cuts at: 5214 5214
Size: 5551
BspHI T'CATG_A
Cuts at: 3440 5170 3440
Size: 1730 3821
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2144 2906 844
Size: 1300 762 3489
Fragments arranged by size:
3489 1300 762
BsrBI CCG'CTC
Cuts at: 1394 1823 2164 3438 1394
Size: 429 341 1274 3507
Fragments arranged by size:
3507 1274 429 341
BsrDI GCAATG_nn'
Cuts at: 65 1248 4046 4220 65
Size: 1183 2798 174 1396
Fragments arranged by size:
2798 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2054 2104 4195 4382 1700
Size: 110 244 50 2091 187 2869
Fragments arranged by size:
2869 2091 244 187 110 50
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5551
BssHII G'CGCG_C
Cuts at: 1925 2149 1925
Size: 224 5327
BssSI C'ACGA_G
Cuts at: 3611 4623 3611
Size: 1012 4539
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2098 794
Size: 1304 4247
BstBI TT'CG_AA
Cuts at: 4355 4355
Size: 5551
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5551
BstXI CCAn_nnnn'nTGG
Cuts at: 2509 2509
Size: 5551
BstYI r'GATC_y
Cuts at: 1972 2881 3248 3633 3650 5102 5188 5200 5546
Size: 909 367 385 17 1452 86 12 346
Cuts at: 5546 1972
Size: 1977
Fragments arranged by size:
1977 1452 909 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4999
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4812
Fragments arranged by size:
4812 683 56
BtsI GCAGTG_nn'
Cuts at: 3067 3861 3881 3067
Size: 794 20 4737
Fragments arranged by size:
4737 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5551
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5516
DraI TTT'AAA
Cuts at: 2855 2855
Size: 5551
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4558 809
Size: 507 3242 1802
Fragments arranged by size:
3242 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2168 2998 3887
Size: 54 1425 50 275 18 338 830 889
Cuts at: 3887 5516 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 830 338 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2168 2998 1537
Size: 275 356 830 4090
Fragments arranged by size:
4090 830 356 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3481 1353
Size: 2128 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4138 4512 4658 387
Size: 1064 138 2549 374 146 1280
Fragments arranged by size:
2549 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2779 2779
Size: 5551
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2343 2813 3614 4211 4998 5266 1239
Size: 393 711 470 801 597 787 268 1524
Fragments arranged by size:
1524 801 787 711 597 470 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2545 2545
Size: 5551
EcoRI G'AATT_C
Cuts at: 2860 3238 2860
Size: 378 5173
FspI TGC'GCA
Cuts at: 4057 4057
Size: 5551
HaeII r_GCGC'y
Cuts at: 1678 4698 1678
Size: 3020 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2482 2514 2600 2632 4192
Size: 32 135 32 910 32 86 32 1560
Cuts at: 4192 4224 4266 4298 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1560 910 135 86 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2895 3141 669
Size: 988 281 957 246 3079
Fragments arranged by size:
3079 988 957 281 246
HindIII A'AGCT_T
Cuts at: 748 2244 748
Size: 1496 4055
HpaI GTT'AAC
Cuts at: 3141 3141
Size: 5551
KpnI G_GTAC'C
Cuts at: 2876 2876
Size: 5551
MfeI C'AATT_G
Cuts at: 3150 3150
Size: 5551
MluI A'CGCG_T
Cuts at: 5427 5427
Size: 5551
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2535 2563 4665 4849 1305
Size: 1230 28 2102 184 2007
Fragments arranged by size:
2102 2007 1230 184 28
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3729
Fragments arranged by size:
3729 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3509 3868 4027 518
Size: 648 2343 359 159 2042
Fragments arranged by size:
2343 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2967 3644 4792 5037 1606
Size: 1361 677 1148 245 2120
Fragments arranged by size:
2120 1361 1148 677 245
NaeI GCC'GGC
Cuts at: 2056 2106 2056
Size: 50 5501
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4999
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5551
NgoMIV G'CCGG_C
Cuts at: 2054 2104 2054
Size: 50 5501
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5551
NotI GC'GGCC_GC
Cuts at: 2998 2998
Size: 5551
NspI r_CATG'y
Cuts at: 1132 1229 2909 4454 1132
Size: 97 1680 1545 2229
Fragments arranged by size:
2229 1680 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5551
PciI A'CATG_T
Cuts at: 4450 4450
Size: 5551
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2722 1108
Size: 1614 3937
PmeI GTTT'AAAC
Cuts at: 2855 2855
Size: 5551
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3620 3652 5133 5165 3620
Size: 32 1481 32 4006
Fragments arranged by size:
4006 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5551
PsiI TTA'TAA
Cuts at: 2522 3121 2522
Size: 599 4952
PstI C_TGCA'G
Cuts at: 830 2103 2903 830
Size: 1273 800 3478
Fragments arranged by size:
3478 1273 800
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5551
SacI G_AGCT'C
Cuts at: 2781 2781
Size: 5551
SalI G'TCGA_C
Cuts at: 1936 2893 1936
Size: 957 4594
SbfI CC_TGCA'GG
Cuts at: 2903 2903
Size: 5551
ScaI AGT'ACT
Cuts at: 3799 5511 3799
Size: 1712 3839
SfcI C'TryA_G
Cuts at: 826 1045 2099 2899 4034 4715 4906 826
Size: 219 1054 800 1135 681 191 1471
Fragments arranged by size:
1471 1135 1054 800 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5551
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5551
SmaI CCC'GGG
Cuts at: 1872 2878 1872
Size: 1006 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2828 3655 4556 4818 820
Size: 197 926 885 827 901 262 1553
Fragments arranged by size:
1553 926 901 885 827 262 197
SnaBI TAC'GTA
Cuts at: 493 3342 493
Size: 2849 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5551
SphI G_CATG'C
Cuts at: 1132 2909 1132
Size: 1777 3774
SspI AAT'ATT
Cuts at: 5 52 3475 5
Size: 47 3423 2081
Fragments arranged by size:
3423 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5551
StyI C'CwwG_G
Cuts at: 513 1065 2694 2990 513
Size: 552 1629 296 3074
Fragments arranged by size:
3074 1629 552 296
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2754 3578 3595 3748 3933 4378 325
Size: 1255 1174 824 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1174 824 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2805 3797 5509
Size: 274 80 33 51 1443 828 992 1712
Cuts at: 5509 96
Size: 138
Fragments arranged by size:
1712 1443 992 828 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5511 5543 5511
Size: 32 5519
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5551
XbaI T'CTAG_A
Cuts at: 2887 2887
Size: 5551
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5551
XmaI C'CCGG_G
Cuts at: 1870 2876 1870
Size: 1006 4545
XmnI GAAnn'nnTTC
Cuts at: 3680 3680
Size: 5551
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3359 276
Size: 53 83 186 936 1825 2468
Fragments arranged by size:
2468 1825 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoNI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI