Restriction map for
pFN21AE1428
(Circular) (Six-base) MAPSORT of: pFN21AE1428.seq Check: 9805 from: 1 to: 5635
pFN21AE1428 5635 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2143 2143
Size: 5635
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3445 278
Size: 53 83 186 936 1909 2468
Fragments arranged by size:
2468 1909 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2978 1937
Size: 1041 4594
Acc65I G'GTAC_C
Cuts at: 2956 2956
Size: 5635
AclI AA'CG_TT
Cuts at: 1563 3762 4135 1563
Size: 2199 373 3063
Fragments arranged by size:
3063 2199 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3698 5082 5350 1632
Size: 2066 1384 268 1917
Fragments arranged by size:
2066 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5438
AflIII A'CryG_T
Cuts at: 1203 2337 4534 5511 1203
Size: 1134 2197 977 1327
Fragments arranged by size:
2197 1327 1134 977
AgeI A'CCGG_T
Cuts at: 4466 4466
Size: 5635
AhdI GACnn_n'nnGTC
Cuts at: 2062 4364 2062
Size: 2302 3333
AleI CACnn'nnGTG
Cuts at: 1166 2577 1166
Size: 1411 4224
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2916 1184
Size: 1732 3903
AlwNI CAG_nnn'CTG
Cuts at: 3378 4950 3378
Size: 1572 4063
ApaLI G'TGCA_C
Cuts at: 3692 4848 3692
Size: 1156 4479
ApoI r'AATT_y
Cuts at: 1493 2522 2944 3170 3322 5431 1493
Size: 1029 422 226 152 2109 1697
Fragments arranged by size:
2109 1697 1029 422 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4189 160
Size: 872 3157 1606
Fragments arranged by size:
3157 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
AvaI C'yCGr_G
Cuts at: 1870 1943 2165 2960 1870
Size: 73 222 795 4545
Fragments arranged by size:
4545 795 222 73
BamHI G'GATC_C
Cuts at: 2536 2965 3332 2536
Size: 429 367 4839
Fragments arranged by size:
4839 429 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2457 2956 4411 618
Size: 325 217 627 670 499 1455 1842
Fragments arranged by size:
1842 1455 670 627 499 325 217
BanII G_rGCy'C
Cuts at: 1391 2172 2954 1391
Size: 781 782 4072
Fragments arranged by size:
4072 782 781
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4664
BbvCI CC'TCA_GC
Cuts at: 2363 2453 2363
Size: 90 5545
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3826 3860 1903
Size: 34 1889 34 3678
Fragments arranged by size:
3678 1889 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2768 3527 4737 2768
Size: 759 1210 3666
Fragments arranged by size:
3666 1210 759
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5635
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2349 4246 136
Size: 107 122 71 631 605 677 1897 1525
Fragments arranged by size:
1897 1525 677 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5630 5630
Size: 5635
BlpI GC'TnA_GC
Cuts at: 3052 3052
Size: 5635
Bme1580I G_kGCm'C
Cuts at: 1790 3696 4852 1790
Size: 1906 1156 2573
Fragments arranged by size:
2573 1906 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5635
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2059 2167 2703
Size: 416 53 83 399 24 642 108 536
Cuts at: 2703 3366 4324 442
Size: 663 958 1753
Fragments arranged by size:
1753 958 663 642 536 416 399 108
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5635
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2260 2822 2260
Size: 562 5073
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2174 2393 2444 4295 1239
Size: 935 219 51 1851 2579
Fragments arranged by size:
2579 1851 935 219 51
Bpu10I CC'TnA_GC
Cuts at: 2363 2453 2363
Size: 90 5545
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3760 4625 4923 3760
Size: 865 298 4472
Fragments arranged by size:
4472 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2795 4298 882
Size: 617 1296 1503 2219
Fragments arranged by size:
2219 1503 1296 617
BsaAI yAC'GTr
Cuts at: 493 1204 2340 3426 493
Size: 711 1136 1086 2702
Fragments arranged by size:
2702 1136 1086 711
BsaBI GATnn'nnATC
Cuts at: 2672 5629 2672
Size: 2957 2678
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3442 3824 4459 275
Size: 53 83 186 936 1909 382 635 1451
Fragments arranged by size:
1909 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4068 4466 4740 4887 5298 4068
Size: 398 274 147 411 4405
Fragments arranged by size:
4405 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2324 2354 1178
Size: 30 165 30 921 30 4459
Fragments arranged by size:
4459 921 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5635
BseYI C'CCAG_C
Cuts at: 2070 2126 2294 2654 4838 5334 2070
Size: 56 168 360 2184 496 2371
Fragments arranged by size:
2371 2184 496 360 168 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2575 2806 1163
Size: 573 839 231 3992
Fragments arranged by size:
3992 839 573 231
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3085 3846 4874 1540
Size: 275 186 1084 761 1028 2301
Fragments arranged by size:
2301 1084 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2190 2409 3696 3781 4852 2190
Size: 219 1287 85 1071 2973
Fragments arranged by size:
2973 1287 1071 219 85
BsmI GAATG_Cn'
Cuts at: 3146 3239 3146
Size: 93 5542
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2172 2190 2409 2954 3696 3781 4852
Size: 399 382 18 219 545 742 85 1071
Cuts at: 4852 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 545 399 382 219 85
18
BspEI T'CCGG_A
Cuts at: 5298 5298
Size: 5635
BspHI T'CATG_A
Cuts at: 3524 5254 3524
Size: 1730 3905
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2143 2990 844
Size: 1299 847 3489
Fragments arranged by size:
3489 1299 847
BsrBI CCG'CTC
Cuts at: 1394 1823 3522 1394
Size: 429 1699 3507
Fragments arranged by size:
3507 1699 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4130 4304 65
Size: 1183 2882 174 1396
Fragments arranged by size:
2882 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4279 4466 1700
Size: 110 2469 187 2869
Fragments arranged by size:
2869 2469 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5635
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5635
BssSI C'ACGA_G
Cuts at: 3695 4707 3695
Size: 1012 4623
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5635
BstBI TT'CG_AA
Cuts at: 4439 4439
Size: 5635
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5635
BstYI r'GATC_y
Cuts at: 1972 2536 2965 3332 3717 3734 5186 5272 5284
Size: 564 429 367 385 17 1452 86 12
Cuts at: 5284 5630 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 564 429 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4144
Fragments arranged by size:
4144 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4896
Fragments arranged by size:
4896 683 56
BtsI GCAGTG_nn'
Cuts at: 2587 3151 3945 3965 2587
Size: 564 794 20 4257
Fragments arranged by size:
4257 794 564 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5635
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5600
DraI TTT'AAA
Cuts at: 2939 2939
Size: 5635
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4642 809
Size: 507 3326 1802
Fragments arranged by size:
3326 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2350 2709 3082
Size: 54 1425 50 275 18 520 359 373
Cuts at: 3082 3971 5600 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 520 373 359 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3082 1537
Size: 275 1270 4090
Fragments arranged by size:
4090 1270 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2665 2904 3565 1353
Size: 1312 239 661 3423
Fragments arranged by size:
3423 1312 661 239
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4222 4596 4742 387
Size: 1064 138 2633 374 146 1280
Fragments arranged by size:
2633 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2174 2393 2444 3698 4295 5082 5350
Size: 393 542 219 51 1254 597 787 268
Cuts at: 5350 1239
Size: 1524
Fragments arranged by size:
1524 1254 787 597 542 393 268 219
51
EcoRI G'AATT_C
Cuts at: 2944 3322 2944
Size: 378 5257
FspI TGC'GCA
Cuts at: 4141 4141
Size: 5635
HaeII r_GCGC'y
Cuts at: 1678 4782 1678
Size: 3104 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2048 2080 2430 2462 2749
Size: 32 135 32 476 32 350 32 287
Cuts at: 2749 2781 4276 4308 4350 4382 1373
Size: 32 1495 32 42 32 2626
Fragments arranged by size:
2626 1495 476 350 287 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2979 3225 669
Size: 988 281 1041 246 3079
Fragments arranged by size:
3079 1041 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5635
HpaI GTT'AAC
Cuts at: 3225 3225
Size: 5635
KpnI G_GTAC'C
Cuts at: 2960 2960
Size: 5635
MfeI C'AATT_G
Cuts at: 3234 3234
Size: 5635
MluI A'CGCG_T
Cuts at: 5511 5511
Size: 5635
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4749 4933 1305
Size: 3444 184 2007
Fragments arranged by size:
3444 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2352 10
Size: 54 1425 343 520 3293
Fragments arranged by size:
3293 1425 520 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2577 2898 3593 3952 4111 518
Size: 648 1411 321 695 359 159 2042
Fragments arranged by size:
2042 1411 695 648 359 321 159
MspA1I CmG'CkG
Cuts at: 1606 2102 2328 2466 3051 3728 4876 5121 1606
Size: 496 226 138 585 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 585 496 245 226 138
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4144
Fragments arranged by size:
4144 939 552
NdeI CA'TA_TG
Cuts at: 387 2085 387
Size: 1698 3937
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5635
NotI GC'GGCC_GC
Cuts at: 3082 3082
Size: 5635
NspI r_CATG'y
Cuts at: 1132 1229 2217 2993 4538 1132
Size: 97 988 776 1545 2229
Fragments arranged by size:
2229 1545 988 776 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5635
PciI A'CATG_T
Cuts at: 4534 4534
Size: 5635
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2253 1108
Size: 1145 4490
PfoI T'CCnGG_A
Cuts at: 2190 2430 2190
Size: 240 5395
PmeI GTTT'AAAC
Cuts at: 2939 2939
Size: 5635
PmlI CAC'GTG
Cuts at: 2340 2340
Size: 5635
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3704 3736 5217 5249 3704
Size: 32 1481 32 4090
Fragments arranged by size:
4090 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5635
PsiI TTA'TAA
Cuts at: 3205 3205
Size: 5635
PspXI vC'TCGA_Gb
Cuts at: 2165 2165
Size: 5635
PstI C_TGCA'G
Cuts at: 830 2140 2747 2987 830
Size: 1310 607 240 3478
Fragments arranged by size:
3478 1310 607 240
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5635
PvuII CAG'CTG
Cuts at: 2102 2466 2102
Size: 364 5271
SalI G'TCGA_C
Cuts at: 1936 2977 1936
Size: 1041 4594
SapI GCTCTTCn'nnn_
Cuts at: 2665 2904 2665
Size: 239 5396
SbfI CC_TGCA'GG
Cuts at: 2987 2987
Size: 5635
ScaI AGT'ACT
Cuts at: 3883 5595 3883
Size: 1712 3923
SfcI C'TryA_G
Cuts at: 826 1045 2136 2299 2703 2724 2743 2983 4118
Size: 219 1091 163 404 21 19 240 1135
Cuts at: 4118 4799 4990 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1091 681 404 240 219 191
163 21 19
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5635
SmaI CCC'GGG
Cuts at: 1872 2962 1872
Size: 1090 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2165 3739 4640 4902 820
Size: 197 926 222 1574 901 262 1553
Fragments arranged by size:
1574 1553 926 901 262 222 197
SnaBI TAC'GTA
Cuts at: 493 3426 493
Size: 2933 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5635
SphI G_CATG'C
Cuts at: 1132 2993 1132
Size: 1861 3774
SspI AAT'ATT
Cuts at: 5 52 3559 5
Size: 47 3507 2081
Fragments arranged by size:
3507 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5635
StyI C'CwwG_G
Cuts at: 513 1065 2004 2075 3074 513
Size: 552 939 71 999 3074
Fragments arranged by size:
3074 999 939 552 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2297 3662 3679 3832 4017 4462 325
Size: 1255 717 1365 17 153 185 445 1498
Fragments arranged by size:
1498 1365 1255 717 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2334 2616 3881
Size: 274 80 33 51 1443 357 282 1265
Cuts at: 3881 5593 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1265 357 282 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2324 2356 5595 5627 2324
Size: 32 3239 32 2332
Fragments arranged by size:
3239 2332 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5635
XbaI T'CTAG_A
Cuts at: 2971 2971
Size: 5635
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2573 2573
Size: 5635
XhoI C'TCGA_G
Cuts at: 1943 2165 1943
Size: 222 5413
XmaI C'CCGG_G
Cuts at: 1870 2960 1870
Size: 1090 4545
XmnI GAAnn'nnTTC
Cuts at: 3764 3764
Size: 5635
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3443 276
Size: 53 83 186 936 1909 2468
Fragments arranged by size:
2468 1909 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PpuMI PspOMI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI