Restriction map for
pFN21AE1427
(Circular) (Six-base) MAPSORT of: pFN21AE1427.seq Check: 9538 from: 1 to: 8650
pFN21AE1427 8650 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3279 3658 3279
Size: 379 8271
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6460 278
Size: 53 83 186 936 4924 2468
Fragments arranged by size:
4924 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3526 4277 5993 1937
Size: 1589 751 1716 4594
Fragments arranged by size:
4594 1716 1589 751
Acc65I G'GTAC_C
Cuts at: 5971 5971
Size: 8650
AclI AA'CG_TT
Cuts at: 1563 6777 7150 1563
Size: 5214 373 3063
Fragments arranged by size:
5214 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2120 2319 2831 3326 4098 4302 4889 5688
Size: 488 199 512 495 772 204 587 799
Cuts at: 5688 6713 8097 8365 1632
Size: 1025 1384 268 1917
Fragments arranged by size:
1917 1384 1025 799 772 587 512 495
488 268 204 199
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8453
AflIII A'CryG_T
Cuts at: 1203 2019 7549 8526 1203
Size: 816 5530 977 1327
Fragments arranged by size:
5530 1327 977 816
AgeI A'CCGG_T
Cuts at: 7481 7481
Size: 8650
AhdI GACnn_n'nnGTC
Cuts at: 2554 7379 2554
Size: 4825 3825
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4015 5447 1184
Size: 2831 1432 4387
Fragments arranged by size:
4387 2831 1432
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2835 2867 3159 3191 2835
Size: 32 292 32 8294
Fragments arranged by size:
8294 292 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2679 2704 2731 2972 3116 3271 3388 3595 3943
Size: 25 27 241 144 155 117 207 348
Cuts at: 3943 5171 5230 5812 5869 6393 7965 2679
Size: 1228 59 582 57 524 1572 3364
Fragments arranged by size:
3364 1572 1228 582 524 348 241 207
155 144 117 59 57 27 25
ApaLI G'TGCA_C
Cuts at: 6707 7863 6707
Size: 1156 7494
ApoI r'AATT_y
Cuts at: 1493 2143 5371 5959 6185 6337 8446 1493
Size: 650 3228 588 226 152 2109 1697
Fragments arranged by size:
3228 2109 1697 650 588 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7204 160
Size: 872 6172 1606
Fragments arranged by size:
6172 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8650
AvaI C'yCGr_G
Cuts at: 1870 1943 2445 3035 3138 4679 5975 1870
Size: 73 502 590 103 1541 1296 4545
Fragments arranged by size:
4545 1541 1296 590 502 103 73
AvrII C'CTAG_G
Cuts at: 5885 5885
Size: 8650
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5801 5834 5801
Size: 33 8617
BamHI G'GATC_C
Cuts at: 5134 5497 5980 6347 5134
Size: 363 483 367 7437
Fragments arranged by size:
7437 483 367 363
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2591 3124 4650 4912 5825
Size: 325 217 627 804 533 1526 262 913
Cuts at: 5825 5971 7426 618
Size: 146 1455 1842
Fragments arranged by size:
1842 1526 1455 913 804 627 533 325
262 217 146
BanII G_rGCy'C
Cuts at: 1391 2265 3873 3925 4212 4815 5969 1391
Size: 874 1608 52 287 603 1154 4072
Fragments arranged by size:
4072 1608 1154 874 603 287 52
BbeI G_GCGC'C
Cuts at: 4916 4916
Size: 8650
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2307 3440 4086 4187 928
Size: 971 408 1133 646 101 5391
Fragments arranged by size:
5391 1133 971 646 408 101
BbvCI CC'TCA_GC
Cuts at: 2138 2253 2934 2138
Size: 115 681 7854
Fragments arranged by size:
7854 681 115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6841 6875 1903
Size: 34 4904 34 3678
Fragments arranged by size:
4904 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4862 5636 6542 7752 4862
Size: 774 906 1210 5760
Fragments arranged by size:
5760 1210 906 774
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8650
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5358 5549 7261
Size: 107 122 71 631 605 3686 191 1712
Cuts at: 7261 136
Size: 1525
Fragments arranged by size:
3686 1712 1525 631 605 191 122 107
71
BglII A'GATC_T
Cuts at: 8645 8645
Size: 8650
BlpI GC'TnA_GC
Cuts at: 4781 5465 6067 4781
Size: 684 602 7364
Fragments arranged by size:
7364 684 602
Bme1580I G_kGCm'C
Cuts at: 1790 3129 5386 5443 5589 6711 7867 1790
Size: 1339 2257 57 146 1122 1156 2573
Fragments arranged by size:
2573 2257 1339 1156 1122 146 57
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8650
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2320 4810 5583
Size: 416 53 83 399 24 903 2490 773
Cuts at: 5583 6381 7339 442
Size: 798 958 1753
Fragments arranged by size:
2490 1753 958 903 798 773 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4605 1056
Size: 3549 5101
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3145 3145
Size: 8650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2493 3138 3396 3439 3542 3659 3817 3990
Size: 1254 645 258 43 103 117 158 173
Cuts at: 3990 4445 7310 1239
Size: 455 2865 2579
Fragments arranged by size:
2865 2579 1254 645 455 258 173 158
117 103 43
Bpu10I CC'TnA_GC
Cuts at: 2085 2138 2253 2934 3440 4094 4734 4826 2085
Size: 53 115 681 506 654 640 92 5909
Fragments arranged by size:
5909 681 654 640 506 115 92 53
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2627 2999 4133 6775 7640 7938 2627
Size: 372 1134 2642 865 298 3339
Fragments arranged by size:
3339 2642 1134 865 372 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3272 5727 7313 882
Size: 617 1773 2455 1586 2219
Fragments arranged by size:
2455 2219 1773 1586 617
BsaAI yAC'GTr
Cuts at: 493 1204 2020 6441 493
Size: 711 816 4421 2702
Fragments arranged by size:
4421 2702 816 711
BsaBI GATnn'nnATC
Cuts at: 8644 8644
Size: 8650
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4913 6457 6839 7474
Size: 53 83 186 936 3380 1544 382 635
Cuts at: 7474 275
Size: 1451
Fragments arranged by size:
3380 1544 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3147 5775 7083 7481 7755 7902 8313 3147
Size: 2628 1308 398 274 147 411 3484
Fragments arranged by size:
3484 2628 1308 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4643 4673 4802 4832 5397
Size: 30 165 30 3240 30 129 30 565
Cuts at: 5397 5427 1178
Size: 30 4401
Fragments arranged by size:
4401 3240 565 165 129 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2390 2537 2951 3036 3197 3254 3303 3658
Size: 1204 147 414 85 161 57 49 355
Cuts at: 3658 4571 4697 5696 1186
Size: 913 126 999 4140
Fragments arranged by size:
4140 1204 999 913 414 355 161 147
126 85 57 49
BseYI C'CCAG_C
Cuts at: 3099 3199 4029 4839 4886 5285 5598 5706 5739
Size: 100 830 810 47 399 313 108 33
Cuts at: 5739 7853 8349 3099
Size: 2114 496 3400
Fragments arranged by size:
3400 2114 830 810 496 399 313 108
100 47 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3117 4124 4428 4677 5533 1163
Size: 573 1381 1007 304 249 856 4280
Fragments arranged by size:
4280 1381 1007 856 573 304 249
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6100 6861 7889 1540
Size: 275 186 4099 761 1028 2301
Fragments arranged by size:
4099 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2086 2265 2459 3215 4950 6711 6796 7867 2086
Size: 179 194 756 1735 1761 85 1071 2869
Fragments arranged by size:
2869 1761 1735 1071 756 194 179 85
BsmI GAATG_Cn'
Cuts at: 2353 3173 3428 6161 6254 2353
Size: 820 255 2733 93 4749
Fragments arranged by size:
4749 2733 820 255 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4399 5197 4399
Size: 798 7852
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2086 2265 2459 3129 3215 3873 3925
Size: 399 296 179 194 670 86 658 52
Cuts at: 3925 4212 4815 4950 5386 5443 5589 5969 6711
Size: 287 603 135 436 57 146 380 742
Cuts at: 6711 6796 7867 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 670 658 603 436 399
380 296 287 194 179 146 135 86
85 57 52
BspEI T'CCGG_A
Cuts at: 5775 8313 5775
Size: 2538 6112
BspHI T'CATG_A
Cuts at: 6539 8269 6539
Size: 1730 6920
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2980 3279 3658 3844 4036 4338 5050 5575
Size: 2136 299 379 186 192 302 712 525
Cuts at: 5575 6005 844
Size: 430 3489
Fragments arranged by size:
3489 2136 712 525 430 379 302 299
192 186
BsrBI CCG'CTC
Cuts at: 1394 1823 6537 1394
Size: 429 4714 3507
Fragments arranged by size:
4714 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2316 5542 5641 5766 7145 7319 65
Size: 1183 1068 3226 99 125 1379 174 1396
Fragments arranged by size:
3226 1396 1379 1183 1068 174 125 99
BsrFI r'CCGG_y
Cuts at: 1700 1810 7294 7481 1700
Size: 110 5484 187 2869
Fragments arranged by size:
5484 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2058 96
Size: 1962 6688
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8650
BssSI C'ACGA_G
Cuts at: 6710 7722 6710
Size: 1012 7638
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2704 3271 3748 794
Size: 1910 567 477 5696
Fragments arranged by size:
5696 1910 567 477
BstBI TT'CG_AA
Cuts at: 7454 7454
Size: 8650
BstEII G'GTnAC_C
Cuts at: 1184 4447 1184
Size: 3263 5387
BstXI CCAn_nnnn'nTGG
Cuts at: 2467 3004 5606 2467
Size: 537 2602 5511
Fragments arranged by size:
5511 2602 537
BstYI r'GATC_y
Cuts at: 1972 2271 3072 3384 5134 5497 5611 5980 6347
Size: 299 801 312 1750 363 114 369 367
Cuts at: 6347 6732 6749 8201 8287 8299 8645 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1750 1452 801 385 369 367 363
346 312 299 114 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3048 4670 5027 5616 5930 3048
Size: 1622 357 589 314 5768
Fragments arranged by size:
5768 1622 589 357 314
BtgI C'CryG_G
Cuts at: 513 1065 2004 2720 3792 4467 4662 4791 513
Size: 552 939 716 1072 675 195 129 4372
Fragments arranged by size:
4372 1072 939 716 675 552 195 129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7911
Fragments arranged by size:
7911 683 56
BtsI GCAGTG_nn'
Cuts at: 3469 3941 4002 4363 5441 5521 5589 5867 5905
Size: 472 61 361 1078 80 68 278 38
Cuts at: 5905 6166 6960 6980 3469
Size: 261 794 20 5139
Fragments arranged by size:
5139 1078 794 472 361 278 261 80
68 61 38 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8650
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2395 2430 529
Size: 35 1831 35 6749
Fragments arranged by size:
6749 1831 35 35
DraI TTT'AAA
Cuts at: 5954 5954
Size: 8650
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4434 7657 809
Size: 507 3118 3223 1802
Fragments arranged by size:
3223 3118 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2738 3066 4788
Size: 54 1425 50 275 18 908 328 1722
Cuts at: 4788 4794 5019 6097 6986 8615 8
Size: 6 225 1078 889 1629 43
Fragments arranged by size:
1722 1629 1425 1078 908 889 328 275
225 54 50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 6097 1537
Size: 275 4285 4090
Fragments arranged by size:
4285 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3086 4266 6580 1353
Size: 1733 1180 2314 3423
Fragments arranged by size:
3423 2314 1733 1180
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7237 7611 7757 387
Size: 1064 138 5648 374 146 1280
Fragments arranged by size:
5648 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2263 2263
Size: 8650
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2120 2319 2493 2831 3138 3326 3396
Size: 393 488 199 174 338 307 188 70
Cuts at: 3396 3439 3542 3659 3817 3990 4098 4302 4445
Size: 43 103 117 158 173 108 204 143
Cuts at: 4445 4889 5688 6713 7310 8097 8365 1239
Size: 444 799 1025 597 787 268 1524
Fragments arranged by size:
1524 1025 799 787 597 488 444 393
338 307 268 204 199 188 174 173
158 143 117 108 103 70 43
EcoNI CCTnn'n_nnAGG
Cuts at: 2405 4148 5786 2405
Size: 1743 1638 5269
Fragments arranged by size:
5269 1743 1638
EcoO109I rG'GnC_Cy
Cuts at: 2647 2814 2851 2969 3436 3579 3592 4183 5275
Size: 167 37 118 467 143 13 591 1092
Cuts at: 5275 5564 5663 2647
Size: 289 99 5634
Fragments arranged by size:
5634 1092 591 467 289 167 143 118
99 37 13
EcoRI G'AATT_C
Cuts at: 5371 5959 6337 5371
Size: 588 378 7684
Fragments arranged by size:
7684 588 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4724 4724
Size: 8650
FspI TGC'GCA
Cuts at: 5348 7156 5348
Size: 1808 6842
HaeII r_GCGC'y
Cuts at: 1678 2125 4916 7797 1678
Size: 447 2791 2881 2531
Fragments arranged by size:
2881 2791 2531 447
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2540 2572 3369 3401 4673
Size: 32 135 32 968 32 797 32 1272
Cuts at: 4673 4705 5126 5158 5234 5266 5397 5429 5825
Size: 32 421 32 76 32 131 32 396
Cuts at: 5825 5857 7291 7323 7365 7397 1373
Size: 32 1434 32 42 32 2626
Fragments arranged by size:
2626 1434 1272 968 797 421 396 135
131 76 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4524 5235 5994 6240 669
Size: 988 281 2586 711 759 246 3079
Fragments arranged by size:
3079 2586 988 759 711 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8650
HpaI GTT'AAC
Cuts at: 6240 6240
Size: 8650
KasI G'GCGC_C
Cuts at: 4912 4912
Size: 8650
KpnI G_GTAC'C
Cuts at: 5975 5975
Size: 8650
MfeI C'AATT_G
Cuts at: 6249 6249
Size: 8650
MluI A'CGCG_T
Cuts at: 8526 8526
Size: 8650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2004 5265 5440 7764 7948 1305
Size: 699 3261 175 2324 184 2007
Fragments arranged by size:
3261 2324 2007 699 184 175
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2740 4790 4796 10
Size: 54 1425 343 908 2050 6 3864
Fragments arranged by size:
3864 2050 1425 908 343 54 6
MslI CAynn'nnrTG
Cuts at: 518 1166 4386 6608 6967 7126 518
Size: 648 3220 2222 359 159 2042
Fragments arranged by size:
3220 2222 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2546 2626 3099 3208 3364 3459 5579 5602
Size: 940 80 473 109 156 95 2120 23
Cuts at: 5602 5710 6066 6743 7891 8136 1606
Size: 108 356 677 1148 245 2120
Fragments arranged by size:
2120 2120 1148 940 677 473 356 245
156 109 108 95 80 23
NarI GG'CG_CC
Cuts at: 4913 4913
Size: 8650
NcoI C'CATG_G
Cuts at: 513 1065 2004 3792 4467 4662 4791 513
Size: 552 939 1788 675 195 129 4372
Fragments arranged by size:
4372 1788 939 675 552 195 129
NdeI CA'TA_TG
Cuts at: 387 2199 3998 387
Size: 1812 1799 5039
Fragments arranged by size:
5039 1812 1799
NheI G'CTAG_C
Cuts at: 1052 4601 1052
Size: 3549 5101
NotI GC'GGCC_GC
Cuts at: 6097 6097
Size: 8650
NspI r_CATG'y
Cuts at: 1132 1229 4391 4496 5730 6008 7553 1132
Size: 97 3162 105 1234 278 1545 2229
Fragments arranged by size:
3162 2229 1545 1234 278 105 97
PasI CC'CwG_GG
Cuts at: 1348 4762 5821 1348
Size: 3414 1059 4177
Fragments arranged by size:
4177 3414 1059
PciI A'CATG_T
Cuts at: 7549 7549
Size: 8650
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2023 2878 4468 4473 4768 5947 1108
Size: 915 855 1590 5 295 1179 3811
Fragments arranged by size:
3811 1590 1179 915 855 295 5
PmeI GTTT'AAAC
Cuts at: 5954 5954
Size: 8650
PmlI CAC'GTG
Cuts at: 2020 2020
Size: 8650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6719 6751 8232 8264 6719
Size: 32 1481 32 7105
Fragments arranged by size:
7105 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2647 2814 2851 2969 3436 3579 3592 5275 5564
Size: 167 37 118 467 143 13 1683 289
Cuts at: 5564 5663 2647
Size: 99 5634
Fragments arranged by size:
5634 1683 467 289 167 143 118 99
37 13
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8650
PsiI TTA'TAA
Cuts at: 6220 6220
Size: 8650
PstI C_TGCA'G
Cuts at: 830 2700 3464 3669 4122 4486 4513 5061 5448
Size: 1870 764 205 453 364 27 548 387
Cuts at: 5448 5584 5912 6002 830
Size: 136 328 90 3478
Fragments arranged by size:
3478 1870 764 548 453 387 364 328
205 136 90 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8650
PvuII CAG'CTG
Cuts at: 2626 3099 3364 3459 5579 5602 2626
Size: 473 265 95 2120 23 5674
Fragments arranged by size:
5674 2120 473 265 95 23
SacI G_AGCT'C
Cuts at: 2265 2265
Size: 8650
SalI G'TCGA_C
Cuts at: 1936 5992 1936
Size: 4056 4594
SanDI GG'GwC_CC
Cuts at: 2814 2814
Size: 8650
SapI GCTCTTCn'nnn_
Cuts at: 3086 3086
Size: 8650
SbfI CC_TGCA'GG
Cuts at: 2700 3669 6002 2700
Size: 969 2333 5348
Fragments arranged by size:
5348 2333 969
ScaI AGT'ACT
Cuts at: 6898 8610 6898
Size: 1712 6938
SexAI A'CCwGG_T
Cuts at: 2304 4531 2304
Size: 2227 6423
SfcI C'TryA_G
Cuts at: 826 1045 2696 3460 3665 4118 4482 4509 5057
Size: 219 1651 764 205 453 364 27 548
Cuts at: 5057 5444 5580 5908 5998 7133 7814 8005 826
Size: 387 136 328 90 1135 681 191 1471
Fragments arranged by size:
1651 1471 1135 764 681 548 453 387
364 328 219 205 191 136 90 27
SfoI GGC'GCC
Cuts at: 4914 4914
Size: 8650
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8650
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8650
SmaI CCC'GGG
Cuts at: 1872 2447 5977 1872
Size: 575 3530 4545
Fragments arranged by size:
4545 3530 575
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2642 2978 4148 6754 7655 7917
Size: 197 926 699 336 1170 2606 901 262
Cuts at: 7917 820
Size: 1553
Fragments arranged by size:
2606 1553 1170 926 901 699 336 262
197
SnaBI TAC'GTA
Cuts at: 493 6441 493
Size: 5948 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8650
SphI G_CATG'C
Cuts at: 1132 6008 1132
Size: 4876 3774
SrfI GCCC'GGGC
Cuts at: 2447 2447
Size: 8650
SspI AAT'ATT
Cuts at: 5 52 6574 5
Size: 47 6522 2081
Fragments arranged by size:
6522 2081 47
StuI AGG'CCT
Cuts at: 1517 2918 3614 5890 1517
Size: 1401 696 2276 4277
Fragments arranged by size:
4277 2276 1401 696
StyI C'CwwG_G
Cuts at: 513 1065 2004 2406 2886 3792 3916 4038 4218
Size: 552 939 402 480 906 124 122 180
Cuts at: 4218 4467 4662 4791 5885 6089 513
Size: 249 195 129 1094 204 3074
Fragments arranged by size:
3074 1094 939 906 552 480 402 249
204 195 180 129 124 122
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5379 5813 6677 6694 6847 7032 7477
Size: 1255 3799 434 864 17 153 185 445
Cuts at: 7477 325
Size: 1498
Fragments arranged by size:
3799 1498 1255 864 445 434 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2058 2343 6896
Size: 274 80 33 51 1443 81 285 4553
Cuts at: 6896 8608 96
Size: 1712 138
Fragments arranged by size:
4553 1712 1443 285 274 138 81 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4281 4313 4643 4675 8610 8642 4281
Size: 32 330 32 3935 32 4289
Fragments arranged by size:
4289 3935 330 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8650
XbaI T'CTAG_A
Cuts at: 5986 5986
Size: 8650
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3694 4474 4916 5527 3694
Size: 780 442 611 6817
Fragments arranged by size:
6817 780 611 442
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8650
XmaI C'CCGG_G
Cuts at: 1870 2445 5975 1870
Size: 575 3530 4545
Fragments arranged by size:
4545 3530 575
XmnI GAAnn'nnTTC
Cuts at: 6779 6779
Size: 8650
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6458 276
Size: 53 83 186 936 4924 2468
Fragments arranged by size:
4924 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BfrBI BsiWI BstZ17I DraIII EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PspOMI PspXI PsrI RsrII SacII SfiI SwaI