Restriction map for
pFN21AE1426
(Circular) (Six-base) MAPSORT of: pFN21AE1426.seq Check: 5160 from: 1 to: 5962
pFN21AE1426 5962 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3772 278
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2481 3305 1937
Size: 544 824 4594
Fragments arranged by size:
4594 824 544
Acc65I G'GTAC_C
Cuts at: 3283 3283
Size: 5962
AclI AA'CG_TT
Cuts at: 1563 4089 4462 1563
Size: 2526 373 3063
Fragments arranged by size:
3063 2526 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4025 5409 5677 1632
Size: 2393 1384 268 1917
Fragments arranged by size:
2393 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5765
AflIII A'CryG_T
Cuts at: 1203 2745 4861 5838 1203
Size: 1542 2116 977 1327
Fragments arranged by size:
2116 1542 1327 977
AgeI A'CCGG_T
Cuts at: 4793 4793
Size: 5962
AhdI GACnn_n'nnGTC
Cuts at: 4691 4691
Size: 5962
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5962
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5962
AlwNI CAG_nnn'CTG
Cuts at: 3705 5277 3705
Size: 1572 4390
ApaLI G'TGCA_C
Cuts at: 4019 5175 4019
Size: 1156 4806
ApoI r'AATT_y
Cuts at: 1493 2327 3002 3271 3497 3649 5758 1493
Size: 834 675 269 226 152 2109 1697
Fragments arranged by size:
2109 1697 834 675 269 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4516 160
Size: 872 3484 1606
Fragments arranged by size:
3484 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
AvaI C'yCGr_G
Cuts at: 1870 1943 2062 3287 1870
Size: 73 119 1225 4545
Fragments arranged by size:
4545 1225 119 73
BamHI G'GATC_C
Cuts at: 3292 3659 3292
Size: 367 5595
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2077 3283 4738 618
Size: 325 217 627 290 1206 1455 1842
Fragments arranged by size:
1842 1455 1206 627 325 290 217
BanII G_rGCy'C
Cuts at: 1391 2133 3281 1391
Size: 742 1148 4072
Fragments arranged by size:
4072 1148 742
BbeI G_GCGC'C
Cuts at: 2081 2081
Size: 5962
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4991
BbvCI CC'TCA_GC
Cuts at: 2546 2546
Size: 5962
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4153 4187 1903
Size: 34 50 34 2132 34 3678
Fragments arranged by size:
3678 2132 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3854 5064 3854
Size: 1210 4752
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5962
BfrBI ATG'CAT
Cuts at: 2397 2637 2397
Size: 240 5722
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4573 136
Size: 107 122 71 631 605 2901 1525
Fragments arranged by size:
2901 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2167 2355 5957 2167
Size: 188 3602 2172
Fragments arranged by size:
3602 2172 188
BlpI GC'TnA_GC
Cuts at: 3379 3379
Size: 5962
Bme1580I G_kGCm'C
Cuts at: 1790 4023 5179 1790
Size: 2233 1156 2573
Fragments arranged by size:
2573 2233 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5962
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3693 4651 442
Size: 416 53 83 399 24 2276 958 1753
Fragments arranged by size:
2276 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5962
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2406 2406
Size: 5962
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2192 2829 4622 1239
Size: 953 637 1793 2579
Fragments arranged by size:
2579 1793 953 637
Bpu10I CC'TnA_GC
Cuts at: 2258 2546 2778 2258
Size: 288 232 5442
Fragments arranged by size:
5442 288 232
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2290 2568 4087 4952 5250 2290
Size: 278 1519 865 298 3002
Fragments arranged by size:
3002 1519 865 298 278
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2864 4625 882
Size: 617 1365 1761 2219
Fragments arranged by size:
2219 1761 1365 617
BsaAI yAC'GTr
Cuts at: 493 1204 3753 493
Size: 711 2549 2702
Fragments arranged by size:
2702 2549 711
BsaBI GATnn'nnATC
Cuts at: 5956 5956
Size: 5962
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2078 3769 4151 4786
Size: 53 83 186 936 545 1691 382 635
Cuts at: 4786 275
Size: 1451
Fragments arranged by size:
1691 1451 936 635 545 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2105 4395 4793 5067 5214 5625 2105
Size: 2290 398 274 147 411 2442
Fragments arranged by size:
2442 2290 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2165 2195 1178
Size: 30 165 30 762 30 4945
Fragments arranged by size:
4945 762 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2189 1186
Size: 1003 4959
BseYI C'CCAG_C
Cuts at: 2014 2978 5165 5661 2014
Size: 964 2187 496 2315
Fragments arranged by size:
2315 2187 964 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5389
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3412 4173 5201 1540
Size: 275 186 1411 761 1028 2301
Fragments arranged by size:
2301 1411 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4023 4108 5179 4023
Size: 85 1071 4806
Fragments arranged by size:
4806 1071 85
BsmI GAATG_Cn'
Cuts at: 3473 3566 3473
Size: 93 5869
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2133 3281 4023 4108 5179 1391
Size: 399 343 1148 742 85 1071 2174
Fragments arranged by size:
2174 1148 1071 742 399 343 85
BspEI T'CCGG_A
Cuts at: 2105 5625 2105
Size: 3520 2442
BspHI T'CATG_A
Cuts at: 2211 3851 5581 2211
Size: 1640 1730 2592
Fragments arranged by size:
2592 1730 1640
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2738 3050 3317 844
Size: 1894 312 267 3489
Fragments arranged by size:
3489 1894 312 267
BsrBI CCG'CTC
Cuts at: 1394 1823 2894 3849 1394
Size: 429 1071 955 3507
Fragments arranged by size:
3507 1071 955 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4457 4631 65
Size: 1183 3209 174 1396
Fragments arranged by size:
3209 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4606 4793 1700
Size: 110 2796 187 2869
Fragments arranged by size:
2869 2796 187 110
BsrGI T'GTAC_A
Cuts at: 96 2244 3089 96
Size: 2148 845 2969
Fragments arranged by size:
2969 2148 845
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5962
BssSI C'ACGA_G
Cuts at: 2536 4022 5034 2536
Size: 1486 1012 3464
Fragments arranged by size:
3464 1486 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5962
BstBI TT'CG_AA
Cuts at: 4766 4766
Size: 5962
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5962
BstXI CCAn_nnnn'nTGG
Cuts at: 2235 2235
Size: 5962
BstYI r'GATC_y
Cuts at: 1972 2167 2355 3292 3659 4044 4061 5513 5599
Size: 195 188 937 367 385 17 1452 86
Cuts at: 5599 5611 5957 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 937 385 367 346 195 188
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2148 2402 513
Size: 552 1083 254 4073
Fragments arranged by size:
4073 1083 552 254
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5223
Fragments arranged by size:
5223 683 56
BtsI GCAGTG_nn'
Cuts at: 2525 3478 4272 4292 2525
Size: 953 794 20 4195
Fragments arranged by size:
4195 953 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5962
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5927
DraI TTT'AAA
Cuts at: 2461 3266 2461
Size: 805 5157
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4969 809
Size: 507 3653 1802
Fragments arranged by size:
3653 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3161 3409 4298
Size: 54 1425 50 275 18 1331 248 889
Cuts at: 4298 5927 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1331 889 275 248 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3409 1537
Size: 275 1597 4090
Fragments arranged by size:
4090 1597 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2310 2862 2978 3892 1353
Size: 957 552 116 914 3423
Fragments arranged by size:
3423 957 914 552 116
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4549 4923 5069 387
Size: 1064 138 2960 374 146 1280
Fragments arranged by size:
2960 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2192 2829 4025 4622 5409 5677 1239
Size: 393 560 637 1196 597 787 268 1524
Fragments arranged by size:
1524 1196 787 637 597 560 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2777 2777
Size: 5962
EcoO109I rG'GnC_Cy
Cuts at: 2066 2588 2066
Size: 522 5440
EcoRI G'AATT_C
Cuts at: 3271 3649 3271
Size: 378 5584
FspI TGC'GCA
Cuts at: 4468 4468
Size: 5962
HaeII r_GCGC'y
Cuts at: 1678 2081 5109 1678
Size: 403 3028 2531
Fragments arranged by size:
3028 2531 403
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2100 2132 2927 2959 4603
Size: 32 135 32 528 32 795 32 1644
Cuts at: 4603 4635 4677 4709 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1644 795 528 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3306 3552 669
Size: 988 281 1368 246 3079
Fragments arranged by size:
3079 1368 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5962
HpaI GTT'AAC
Cuts at: 3552 3552
Size: 5962
KasI G'GCGC_C
Cuts at: 2077 2077
Size: 5962
KpnI G_GTAC'C
Cuts at: 3287 3287
Size: 5962
MfeI C'AATT_G
Cuts at: 3561 3561
Size: 5962
MluI A'CGCG_T
Cuts at: 5838 5838
Size: 5962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5076 5260 1305
Size: 3771 184 2007
Fragments arranged by size:
3771 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4140
Fragments arranged by size:
4140 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2950 3022 3106 3920 4279 4438 518
Size: 648 1784 72 84 814 359 159 2042
Fragments arranged by size:
2042 1784 814 648 359 159 84 72
MspA1I CmG'CkG
Cuts at: 1606 2051 2913 3150 3378 4055 5203 5448 1606
Size: 445 862 237 228 677 1148 245 2120
Fragments arranged by size:
2120 1148 862 677 445 245 237 228
NarI GG'CG_CC
Cuts at: 2078 2078
Size: 5962
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5410
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5962
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5962
NotI GC'GGCC_GC
Cuts at: 3409 3409
Size: 5962
NruI TCG'CGA
Cuts at: 2073 2444 2073
Size: 371 5591
NsiI A_TGCA'T
Cuts at: 2399 2639 2399
Size: 240 5722
NspI r_CATG'y
Cuts at: 1132 1229 2401 2641 2749 3021 3147 3320 4865
Size: 97 1172 240 108 272 126 173 1545
Cuts at: 4865 1132
Size: 2229
Fragments arranged by size:
2229 1545 1172 272 240 173 126 108
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5962
PciI A'CATG_T
Cuts at: 2745 4861 2745
Size: 2116 3846
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5962
PfoI T'CCnGG_A
Cuts at: 3067 3067
Size: 5962
PmeI GTTT'AAAC
Cuts at: 3266 3266
Size: 5962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4031 4063 5544 5576 4031
Size: 32 1481 32 4417
Fragments arranged by size:
4417 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2066 2066
Size: 5962
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5962
PsiI TTA'TAA
Cuts at: 2346 3532 2346
Size: 1186 4776
PstI C_TGCA'G
Cuts at: 830 2338 2520 2768 2891 3314 830
Size: 1508 182 248 123 423 3478
Fragments arranged by size:
3478 1508 423 248 182 123
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5962
PvuII CAG'CTG
Cuts at: 2913 3150 2913
Size: 237 5725
SalI G'TCGA_C
Cuts at: 1936 3304 1936
Size: 1368 4594
SbfI CC_TGCA'GG
Cuts at: 3314 3314
Size: 5962
ScaI AGT'ACT
Cuts at: 4210 5922 4210
Size: 1712 4250
SfcI C'TryA_G
Cuts at: 826 1045 2334 2482 2516 2764 2887 3310 4445
Size: 219 1289 148 34 248 123 423 1135
Cuts at: 4445 5126 5317 826
Size: 681 191 1471
Fragments arranged by size:
1471 1289 1135 681 423 248 219 191
148 123 34
SfoI GGC'GCC
Cuts at: 2079 2079
Size: 5962
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5962
SmaI CCC'GGG
Cuts at: 1872 2064 3289 1872
Size: 192 1225 4545
Fragments arranged by size:
4545 1225 192
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2305 2583 4066 4967 5229 820
Size: 197 926 362 278 1483 901 262 1553
Fragments arranged by size:
1553 1483 926 901 362 278 262 197
SnaBI TAC'GTA
Cuts at: 493 3753 493
Size: 3260 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5962
SphI G_CATG'C
Cuts at: 1132 2401 3320 1132
Size: 1269 919 3774
Fragments arranged by size:
3774 1269 919
SspI AAT'ATT
Cuts at: 5 52 2234 3886 5
Size: 47 2182 1652 2081
Fragments arranged by size:
2182 2081 1652 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5962
StyI C'CwwG_G
Cuts at: 513 1065 3401 513
Size: 552 2336 3074
Fragments arranged by size:
3074 2336 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3989 4006 4159 4344 4789 325
Size: 1255 2409 17 153 185 445 1498
Fragments arranged by size:
2409 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2218 2244 3089
Size: 274 80 33 51 1443 241 26 845
Cuts at: 3089 4208 5920 96
Size: 1119 1712 138
Fragments arranged by size:
1712 1443 1119 845 274 241 138 80
51 33 26
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5922 5954 5922
Size: 32 5930
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5962
XbaI T'CTAG_A
Cuts at: 3298 3298
Size: 5962
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2840 2011
Size: 829 5133
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5962
XmaI C'CCGG_G
Cuts at: 1870 2062 3287 1870
Size: 192 1225 4545
Fragments arranged by size:
4545 1225 192
XmnI GAAnn'nnTTC
Cuts at: 4091 4091
Size: 5962
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3770 276
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NruI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI NaeI NgoMIV PacI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI