Restriction map for
pFN21AE1423
(Circular) (Six-base) MAPSORT of: pFN21AE1423.seq Check: 6453 from: 1 to: 6049
pFN21AE1423 6049 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2030 2030
Size: 6049
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3859 278
Size: 53 83 186 936 2323 2468
Fragments arranged by size:
2468 2323 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2537 3392 1937
Size: 600 855 4594
Fragments arranged by size:
4594 855 600
Acc65I G'GTAC_C
Cuts at: 3370 3370
Size: 6049
AclI AA'CG_TT
Cuts at: 1563 4176 4549 1563
Size: 2613 373 3063
Fragments arranged by size:
3063 2613 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2384 4112 5496 5764 1632
Size: 752 1728 1384 268 1917
Fragments arranged by size:
1917 1728 1384 752 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5852
AflIII A'CryG_T
Cuts at: 1203 2185 4948 5925 1203
Size: 982 2763 977 1327
Fragments arranged by size:
2763 1327 982 977
AgeI A'CCGG_T
Cuts at: 4880 4880
Size: 6049
AhdI GACnn_n'nnGTC
Cuts at: 4778 4778
Size: 6049
AleI CACnn'nnGTG
Cuts at: 1166 3089 1166
Size: 1923 4126
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6049
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3170 3202 3239 3271 3170
Size: 32 37 32 5948
Fragments arranged by size:
5948 37 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3792 5364 3792
Size: 1572 4477
ApaI G_GGCC'C
Cuts at: 2746 3172 2746
Size: 426 5623
ApaLI G'TGCA_C
Cuts at: 2389 3218 4106 5262 2389
Size: 829 888 1156 3176
Fragments arranged by size:
3176 1156 888 829
ApoI r'AATT_y
Cuts at: 1493 2507 3358 3584 3736 5845 1493
Size: 1014 851 226 152 2109 1697
Fragments arranged by size:
2109 1697 1014 851 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4603 160
Size: 872 3571 1606
Fragments arranged by size:
3571 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6049
AvaI C'yCGr_G
Cuts at: 1870 1943 2193 3374 1870
Size: 73 250 1181 4545
Fragments arranged by size:
4545 1181 250 73
BamHI G'GATC_C
Cuts at: 2367 3379 3746 2367
Size: 1012 367 4670
Fragments arranged by size:
4670 1012 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2782 2974 3042 3091 3370
Size: 325 217 627 995 192 68 49 279
Cuts at: 3370 4825 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 995 627 325 279 217 192
68 49
BanII G_rGCy'C
Cuts at: 1391 2073 2556 2746 3172 3368 1391
Size: 682 483 190 426 196 4072
Fragments arranged by size:
4072 682 483 426 196 190
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5078
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4240 4274 1903
Size: 34 50 34 2219 34 3678
Fragments arranged by size:
3678 2219 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3941 5151 3941
Size: 1210 4839
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6049
BfrBI ATG'CAT
Cuts at: 2016 2016
Size: 6049
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2104 3051 3209
Size: 107 122 71 631 605 432 947 158
Cuts at: 3209 4660 136
Size: 1451 1525
Fragments arranged by size:
1525 1451 947 631 605 432 158 122
107 71
BglII A'GATC_T
Cuts at: 6044 6044
Size: 6049
BlpI GC'TnA_GC
Cuts at: 3137 3466 3137
Size: 329 5720
Bme1580I G_kGCm'C
Cuts at: 1790 2393 2438 2746 2787 2979 3047 3172 3222
Size: 603 45 308 41 192 68 125 50
Cuts at: 3222 4110 5266 1790
Size: 888 1156 2573
Fragments arranged by size:
2573 1156 888 603 308 192 125 68
50 45 41
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6049
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2109 2295 2348
Size: 416 53 83 399 24 692 186 53
Cuts at: 2348 3780 4738 442
Size: 1432 958 1753
Fragments arranged by size:
1753 1432 958 692 416 399 186 83
53 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6049
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2491 2491
Size: 6049
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2234 2609 3308 4709 1239
Size: 995 375 699 1401 2579
Fragments arranged by size:
2579 1401 995 699 375
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4174 5039 5337 4174
Size: 865 298 4886
Fragments arranged by size:
4886 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4712 882
Size: 617 3213 2219
Fragments arranged by size:
3213 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2029 2188 3840 493
Size: 711 825 159 1652 2702
Fragments arranged by size:
2702 1652 825 711 159
BsaBI GATnn'nnATC
Cuts at: 6043 6043
Size: 6049
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3856 4238 4873 275
Size: 53 83 186 936 2323 382 635 1451
Fragments arranged by size:
2323 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4482 4880 5154 5301 5712 4482
Size: 398 274 147 411 4819
Fragments arranged by size:
4819 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2958 2988 3239 3269 3333
Size: 30 165 30 1555 30 251 30 64
Cuts at: 3333 3363 1178
Size: 30 3864
Fragments arranged by size:
3864 1555 251 165 64 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2701 3260 3317 1186
Size: 1515 559 57 3918
Fragments arranged by size:
3918 1515 559 57
BseYI C'CCAG_C
Cuts at: 2064 2521 5252 5748 2064
Size: 457 2731 496 2365
Fragments arranged by size:
2731 2365 496 457
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3137 1163
Size: 573 1401 4075
Fragments arranged by size:
4075 1401 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3150 3499 4260 5288 1540
Size: 275 186 1149 349 761 1028 2301
Fragments arranged by size:
2301 1149 1028 761 349 275 186
BsiHKAI G_wGCw'C
Cuts at: 2073 2393 2595 3222 4110 4195 5266 2073
Size: 320 202 627 888 85 1071 2856
Fragments arranged by size:
2856 1071 888 627 320 202 85
BsmI GAATG_Cn'
Cuts at: 2018 3024 3560 3653 2018
Size: 1006 536 93 4414
Fragments arranged by size:
4414 1006 536 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2393 2438 2556 2595 2746 2787
Size: 399 283 320 45 118 39 151 41
Cuts at: 2787 2979 3047 3172 3222 3368 4110 4195 5266
Size: 192 68 125 50 146 742 85 1071
Cuts at: 5266 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 320 283 192 151
146 125 118 85 68 50 45 41
39
BspEI T'CCGG_A
Cuts at: 5712 5712
Size: 6049
BspHI T'CATG_A
Cuts at: 3938 5668 3938
Size: 1730 4319
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2030 3404 844
Size: 1186 1374 3489
Fragments arranged by size:
3489 1374 1186
BsrBI CCG'CTC
Cuts at: 1394 1823 2470 3936 1394
Size: 429 647 1466 3507
Fragments arranged by size:
3507 1466 647 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2062 2997 4544 4718 65
Size: 1183 814 935 1547 174 1396
Fragments arranged by size:
1547 1396 1183 935 814 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4693 4880 1700
Size: 110 2883 187 2869
Fragments arranged by size:
2883 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6049
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6049
BssSI C'ACGA_G
Cuts at: 2852 4109 5121 2852
Size: 1257 1012 3780
Fragments arranged by size:
3780 1257 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2061 794
Size: 1267 4782
BstBI TT'CG_AA
Cuts at: 4853 4853
Size: 6049
BstEII G'GTnAC_C
Cuts at: 1184 3101 1184
Size: 1917 4132
BstXI CCAn_nnnn'nTGG
Cuts at: 2426 2908 2426
Size: 482 5567
BstYI r'GATC_y
Cuts at: 1972 2209 2235 2367 3379 3746 4131 4148 5600
Size: 237 26 132 1012 367 385 17 1452
Cuts at: 5600 5686 5698 6044 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1012 385 367 346 237 132
86 26 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2317 2544 2962 513
Size: 552 1252 227 418 3600
Fragments arranged by size:
3600 1252 552 418 227
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3139 487
Size: 683 56 1913 3397
Fragments arranged by size:
3397 1913 683 56
BtsI GCAGTG_nn'
Cuts at: 3565 4359 4379 3565
Size: 794 20 5235
Fragments arranged by size:
5235 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6049
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6014
DraI TTT'AAA
Cuts at: 3353 3353
Size: 6049
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5056 809
Size: 507 3740 1802
Fragments arranged by size:
3740 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2811 3108 3496
Size: 54 1425 50 275 18 981 297 388
Cuts at: 3496 4385 6014 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 981 889 388 297 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3496 1537
Size: 275 1684 4090
Fragments arranged by size:
4090 1684 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2267 3979 1353
Size: 914 1712 3423
Fragments arranged by size:
3423 1712 914
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4636 5010 5156 387
Size: 1064 138 3047 374 146 1280
Fragments arranged by size:
3047 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2071 2071
Size: 6049
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2234 2384 2609 3308 4112 4709 5496
Size: 393 602 150 225 699 804 597 787
Cuts at: 5496 5764 1239
Size: 268 1524
Fragments arranged by size:
1524 804 787 699 602 597 393 268
225 150
EcoNI CCTnn'n_nnAGG
Cuts at: 3110 3290 3110
Size: 180 5869
EcoO109I rG'GnC_Cy
Cuts at: 2742 2743 2742
Size: 1 6048
EcoRI G'AATT_C
Cuts at: 2507 3358 3736 2507
Size: 851 378 4820
Fragments arranged by size:
4820 851 378
FspI TGC'GCA
Cuts at: 4555 4555
Size: 6049
HaeII r_GCGC'y
Cuts at: 1678 2668 5196 1678
Size: 990 2528 2531
Fragments arranged by size:
2531 2528 990
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2846 2878 4690 4722 4764
Size: 32 135 32 1274 32 1812 32 42
Cuts at: 4764 4796 1373
Size: 32 2626
Fragments arranged by size:
2626 1812 1274 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2482 2989 3393 3639 669
Size: 988 281 544 507 404 246 3079
Fragments arranged by size:
3079 988 544 507 404 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6049
HpaI GTT'AAC
Cuts at: 3639 3639
Size: 6049
KpnI G_GTAC'C
Cuts at: 3374 3374
Size: 6049
MfeI C'AATT_G
Cuts at: 3648 3648
Size: 6049
MluI A'CGCG_T
Cuts at: 5925 5925
Size: 6049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5163 5347 1305
Size: 3858 184 2007
Fragments arranged by size:
3858 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2813 3110 10
Size: 54 1425 343 981 297 2949
Fragments arranged by size:
2949 1425 981 343 297 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3089 3225 4007 4366 4525 518
Size: 648 1923 136 782 359 159 2042
Fragments arranged by size:
2042 1923 782 648 359 159 136
MspA1I CmG'CkG
Cuts at: 1606 2860 3465 4142 5290 5535 1606
Size: 1254 605 677 1148 245 2120
Fragments arranged by size:
2120 1254 1148 677 605 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5497
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6049
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6049
NotI GC'GGCC_GC
Cuts at: 3496 3496
Size: 6049
NsiI A_TGCA'T
Cuts at: 2018 2018
Size: 6049
NspI r_CATG'y
Cuts at: 1132 1229 3136 3407 4952 1132
Size: 97 1907 271 1545 2229
Fragments arranged by size:
2229 1907 1545 271 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6049
PciI A'CATG_T
Cuts at: 4948 4948
Size: 6049
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6049
PmeI GTTT'AAAC
Cuts at: 3353 3353
Size: 6049
PmlI CAC'GTG
Cuts at: 2029 2029
Size: 6049
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3239 3271 4118 4150 5631 5663 3239
Size: 32 847 32 1481 32 3625
Fragments arranged by size:
3625 1481 847 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6049
PsiI TTA'TAA
Cuts at: 3619 3619
Size: 6049
PspOMI G'GGCC_C
Cuts at: 2742 3168 2742
Size: 426 5623
PstI C_TGCA'G
Cuts at: 830 2839 3401 830
Size: 2009 562 3478
Fragments arranged by size:
3478 2009 562
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6049
PvuII CAG'CTG
Cuts at: 2860 2860
Size: 6049
SacI G_AGCT'C
Cuts at: 2073 2073
Size: 6049
SalI G'TCGA_C
Cuts at: 1936 3391 1936
Size: 1455 4594
SapI GCTCTTCn'nnn_
Cuts at: 2267 2267
Size: 6049
SbfI CC_TGCA'GG
Cuts at: 3401 3401
Size: 6049
ScaI AGT'ACT
Cuts at: 4297 6009 4297
Size: 1712 4337
SexAI A'CCwGG_T
Cuts at: 2778 2778
Size: 6049
SfcI C'TryA_G
Cuts at: 826 1045 2538 2835 3397 4532 5213 5404 826
Size: 219 1493 297 562 1135 681 191 1471
Fragments arranged by size:
1493 1471 1135 681 562 297 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6049
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6049
SmaI CCC'GGG
Cuts at: 1872 2195 3376 1872
Size: 323 1181 4545
Fragments arranged by size:
4545 1181 323
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4153 5054 5316 820
Size: 197 926 2210 901 262 1553
Fragments arranged by size:
2210 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3840 493
Size: 3347 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6049
SphI G_CATG'C
Cuts at: 1132 3136 3407 1132
Size: 2004 271 3774
Fragments arranged by size:
3774 2004 271
SspI AAT'ATT
Cuts at: 5 52 3973 5
Size: 47 3921 2081
Fragments arranged by size:
3921 2081 47
StuI AGG'CCT
Cuts at: 1517 3164 1517
Size: 1647 4402
StyI C'CwwG_G
Cuts at: 513 1065 2979 3111 3488 513
Size: 552 1914 132 377 3074
Fragments arranged by size:
3074 1914 552 377 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2283 2591 3221 4076 4093 4246 4431
Size: 1255 703 308 630 855 17 153 185
Cuts at: 4431 4876 325
Size: 445 1498
Fragments arranged by size:
1498 1255 855 703 630 445 308 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2937 4295 6007
Size: 274 80 33 51 1443 960 1358 1712
Cuts at: 6007 96
Size: 138
Fragments arranged by size:
1712 1443 1358 960 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2958 2990 6009 6041 2958
Size: 32 3019 32 2966
Fragments arranged by size:
3019 2966 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6049
XbaI T'CTAG_A
Cuts at: 3385 3385
Size: 6049
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2426 2426
Size: 6049
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6049
XmaI C'CCGG_G
Cuts at: 1870 2193 3374 1870
Size: 323 1181 4545
Fragments arranged by size:
4545 1181 323
XmnI GAAnn'nnTTC
Cuts at: 4178 4178
Size: 6049
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3857 276
Size: 53 83 186 936 2323 2468
Fragments arranged by size:
2468 2323 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI
PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI
SwaI