Restriction map for
pFN21AE1420
(Circular) (Six-base) MAPSORT of: pFN21AE1420.seq Check: 615 from: 1 to: 5332
pFN21AE1420 5332 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2448 2448
Size: 5332
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3142 278
Size: 53 83 186 936 1606 2468
Fragments arranged by size:
2468 1606 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2675 1937
Size: 738 4594
Acc65I G'GTAC_C
Cuts at: 2145 2653 2145
Size: 508 4824
AclI AA'CG_TT
Cuts at: 1563 3459 3832 1563
Size: 1896 373 3063
Fragments arranged by size:
3063 1896 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3395 4779 5047 1632
Size: 1763 1384 268 1917
Fragments arranged by size:
1917 1763 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5135
AflIII A'CryG_T
Cuts at: 1203 4231 5208 1203
Size: 3028 977 1327
Fragments arranged by size:
3028 1327 977
AgeI A'CCGG_T
Cuts at: 4163 4163
Size: 5332
AhdI GACnn_n'nnGTC
Cuts at: 4061 4061
Size: 5332
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5332
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5332
AlwNI CAG_nnn'CTG
Cuts at: 3075 4647 3075
Size: 1572 3760
ApaI G_GGCC'C
Cuts at: 2332 2332
Size: 5332
ApaLI G'TGCA_C
Cuts at: 3389 4545 3389
Size: 1156 4176
ApoI r'AATT_y
Cuts at: 1493 2641 2867 3019 5128 1493
Size: 1148 226 152 2109 1697
Fragments arranged by size:
2109 1697 1148 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3886 160
Size: 872 2854 1606
Fragments arranged by size:
2854 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5332
AvaI C'yCGr_G
Cuts at: 1870 1943 2478 2657 1870
Size: 73 535 179 4545
Fragments arranged by size:
4545 535 179 73
BamHI G'GATC_C
Cuts at: 2662 3029 2662
Size: 367 4965
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2145 2653 4108 618
Size: 325 217 627 358 508 1455 1842
Fragments arranged by size:
1842 1455 627 508 358 325 217
BanII G_rGCy'C
Cuts at: 1391 2234 2332 2573 2651 1391
Size: 843 98 241 78 4072
Fragments arranged by size:
4072 843 241 98 78
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2057 928
Size: 971 158 4203
Fragments arranged by size:
4203 971 158
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3523 3557 1903
Size: 34 1586 34 3678
Fragments arranged by size:
3678 1586 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2390 3224 4434 2390
Size: 834 1210 3288
Fragments arranged by size:
3288 1210 834
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5332
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2084 3943 136
Size: 107 122 71 631 605 412 1859 1525
Fragments arranged by size:
1859 1525 631 605 412 122 107 71
BglII A'GATC_T
Cuts at: 5327 5327
Size: 5332
BlpI GC'TnA_GC
Cuts at: 2749 2749
Size: 5332
Bme1580I G_kGCm'C
Cuts at: 1790 2332 3393 4549 1790
Size: 542 1061 1156 2573
Fragments arranged by size:
2573 1156 1061 542
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5332
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3063 4021 442
Size: 416 53 83 399 24 1646 958 1753
Fragments arranged by size:
1753 1646 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5332
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2089 2207 3992 1239
Size: 850 118 1785 2579
Fragments arranged by size:
2579 1785 850 118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2408 3457 4322 4620 2408
Size: 1049 865 298 3120
Fragments arranged by size:
3120 1049 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2012 3995 882
Size: 617 513 1983 2219
Fragments arranged by size:
2219 1983 617 513
BsaAI yAC'GTr
Cuts at: 493 1204 3123 493
Size: 711 1919 2702
Fragments arranged by size:
2702 1919 711
BsaBI GATnn'nnATC
Cuts at: 5326 5326
Size: 5332
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3139 3521 4156 275
Size: 53 83 186 936 1606 382 635 1451
Fragments arranged by size:
1606 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3765 4163 4437 4584 4995 3765
Size: 398 274 147 411 4102
Fragments arranged by size:
4102 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5107
Fragments arranged by size:
5107 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2563 1186
Size: 1377 3955
BseYI C'CCAG_C
Cuts at: 2071 2166 2553 2618 4535 5031 2071
Size: 95 387 65 1917 496 2372
Fragments arranged by size:
2372 1917 496 387 95 65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2420 2457 1163
Size: 573 684 37 4038
Fragments arranged by size:
4038 684 573 37
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2782 3543 4571 1540
Size: 275 186 781 761 1028 2301
Fragments arranged by size:
2301 1028 781 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2034 3393 3478 4549 2034
Size: 1359 85 1071 2817
Fragments arranged by size:
2817 1359 1071 85
BsmI GAATG_Cn'
Cuts at: 2843 2936 2843
Size: 93 5239
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2234 2332 2573 2651 3393 3478
Size: 399 244 200 98 241 78 742 85
Cuts at: 3478 4549 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 244 241 200 98
85 78
BspEI T'CCGG_A
Cuts at: 4995 4995
Size: 5332
BspHI T'CATG_A
Cuts at: 3221 4951 3221
Size: 1730 3602
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2448 2687 844
Size: 1604 239 3489
Fragments arranged by size:
3489 1604 239
BsrBI CCG'CTC
Cuts at: 1394 1823 3219 1394
Size: 429 1396 3507
Fragments arranged by size:
3507 1396 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3827 4001 65
Size: 1183 2579 174 1396
Fragments arranged by size:
2579 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3976 4163 1700
Size: 110 2166 187 2869
Fragments arranged by size:
2869 2166 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5332
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5332
BssSI C'ACGA_G
Cuts at: 3392 4404 3392
Size: 1012 4320
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5332
BstBI TT'CG_AA
Cuts at: 4136 4136
Size: 5332
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5332
BstYI r'GATC_y
Cuts at: 1972 2662 3029 3414 3431 4883 4969 4981 5327
Size: 690 367 385 17 1452 86 12 346
Cuts at: 5327 1972
Size: 1977
Fragments arranged by size:
1977 1452 690 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2358 2358
Size: 5332
BtgI C'CryG_G
Cuts at: 513 1065 2004 2422 513
Size: 552 939 418 3423
Fragments arranged by size:
3423 939 552 418
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4593
Fragments arranged by size:
4593 683 56
BtsI GCAGTG_nn'
Cuts at: 2848 3642 3662 2848
Size: 794 20 4518
Fragments arranged by size:
4518 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5332
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5297
DraI TTT'AAA
Cuts at: 2636 2636
Size: 5332
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4339 809
Size: 507 3023 1802
Fragments arranged by size:
3023 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2318 2779 3668
Size: 54 1425 50 275 18 488 461 889
Cuts at: 3668 5297 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 488 461 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2779 1537
Size: 275 967 4090
Fragments arranged by size:
4090 967 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3262 1353
Size: 1909 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3919 4293 4439 387
Size: 1064 138 2330 374 146 1280
Fragments arranged by size:
2330 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2089 2207 3395 3992 4779 5047 1239
Size: 393 457 118 1188 597 787 268 1524
Fragments arranged by size:
1524 1188 787 597 457 393 268 118
EcoO109I rG'GnC_Cy
Cuts at: 2328 2329 2467 2597 2328
Size: 1 138 130 5063
Fragments arranged by size:
5063 138 130 1
EcoRI G'AATT_C
Cuts at: 2641 3019 2641
Size: 378 4954
FspI TGC'GCA
Cuts at: 3838 3838
Size: 5332
HaeII r_GCGC'y
Cuts at: 1678 2220 4479 1678
Size: 542 2259 2531
Fragments arranged by size:
2531 2259 542
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2555 2587 3973 4005 4047
Size: 32 135 32 983 32 1386 32 42
Cuts at: 4047 4079 1373
Size: 32 2626
Fragments arranged by size:
2626 1386 983 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2676 2922 669
Size: 988 281 738 246 3079
Fragments arranged by size:
3079 988 738 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5332
HpaI GTT'AAC
Cuts at: 2922 2922
Size: 5332
KpnI G_GTAC'C
Cuts at: 2149 2657 2149
Size: 508 4824
MfeI C'AATT_G
Cuts at: 2931 2931
Size: 5332
MluI A'CGCG_T
Cuts at: 5208 5208
Size: 5332
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4446 4630 1305
Size: 3141 184 2007
Fragments arranged by size:
3141 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3510
Fragments arranged by size:
3510 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3290 3649 3808 518
Size: 648 2124 359 159 2042
Fragments arranged by size:
2124 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2143 2251 2317 2434 2748 3425 4573 4818
Size: 537 108 66 117 314 677 1148 245
Cuts at: 4818 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 537 314 245 117 108
66
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3841
Fragments arranged by size:
3841 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5332
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5332
NotI GC'GGCC_GC
Cuts at: 2779 2779
Size: 5332
NspI r_CATG'y
Cuts at: 1132 1229 2690 4235 1132
Size: 97 1461 1545 2229
Fragments arranged by size:
2229 1545 1461 97
PasI CC'CwG_GG
Cuts at: 1348 2605 1348
Size: 1257 4075
PciI A'CATG_T
Cuts at: 4231 4231
Size: 5332
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5332
PfoI T'CCnGG_A
Cuts at: 2373 2373
Size: 5332
PmeI GTTT'AAAC
Cuts at: 2636 2636
Size: 5332
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2009 2041 3401 3433 4914 4946 2009
Size: 32 1360 32 1481 32 2395
Fragments arranged by size:
2395 1481 1360 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2467 2597 2467
Size: 130 5202
PshAI GACnn'nnGTC
Cuts at: 1613 2106 1613
Size: 493 4839
PsiI TTA'TAA
Cuts at: 2902 2902
Size: 5332
PspOMI G'GGCC_C
Cuts at: 2328 2328
Size: 5332
PstI C_TGCA'G
Cuts at: 830 2250 2369 2684 830
Size: 1420 119 315 3478
Fragments arranged by size:
3478 1420 315 119
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5332
PvuII CAG'CTG
Cuts at: 2251 2251
Size: 5332
SalI G'TCGA_C
Cuts at: 1936 2674 1936
Size: 738 4594
SanDI GG'GwC_CC
Cuts at: 2467 2467
Size: 5332
SbfI CC_TGCA'GG
Cuts at: 2684 2684
Size: 5332
ScaI AGT'ACT
Cuts at: 3580 5292 3580
Size: 1712 3620
SfcI C'TryA_G
Cuts at: 826 1045 2246 2365 2680 3815 4496 4687 826
Size: 219 1201 119 315 1135 681 191 1471
Fragments arranged by size:
1471 1201 1135 681 315 219 191 119
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5332
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5332
SmaI CCC'GGG
Cuts at: 1872 2659 1872
Size: 787 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2387 3436 4337 4599 820
Size: 197 926 444 1049 901 262 1553
Fragments arranged by size:
1553 1049 926 901 444 262 197
SnaBI TAC'GTA
Cuts at: 493 3123 493
Size: 2630 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5332
SphI G_CATG'C
Cuts at: 1132 2690 1132
Size: 1558 3774
SspI AAT'ATT
Cuts at: 5 52 3256 5
Size: 47 3204 2081
Fragments arranged by size:
3204 2081 47
StuI AGG'CCT
Cuts at: 1517 2363 2511 1517
Size: 846 148 4338
Fragments arranged by size:
4338 846 148
StyI C'CwwG_G
Cuts at: 513 1065 2004 2771 513
Size: 552 939 767 3074
Fragments arranged by size:
3074 939 767 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3359 3376 3529 3714 4159 325
Size: 1255 1779 17 153 185 445 1498
Fragments arranged by size:
1779 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3578 5290 96
Size: 274 80 33 51 1443 1601 1712 138
Fragments arranged by size:
1712 1601 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2079 2111 2526 2558 5292 5324 2079
Size: 32 415 32 2734 32 2087
Fragments arranged by size:
2734 2087 415 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5332
XbaI T'CTAG_A
Cuts at: 2668 2668
Size: 5332
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2321 2321
Size: 5332
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5332
XmaI C'CCGG_G
Cuts at: 1870 2657 1870
Size: 787 4545
XmnI GAAnn'nnTTC
Cuts at: 3461 3461
Size: 5332
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3140 276
Size: 53 83 186 936 1606 2468
Fragments arranged by size:
2468 1606 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII
SacI SacII SapI SexAI SfiI SfoI SrfI SwaI