Restriction map for
pFN21AE1419
(Circular) (Six-base) MAPSORT of: pFN21AE1419.seq Check: 5932 from: 1 to: 5536
pFN21AE1419 5536 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3346 278
Size: 53 83 186 936 1810 2468
Fragments arranged by size:
2468 1810 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2879 1937
Size: 942 4594
Acc65I G'GTAC_C
Cuts at: 2857 2857
Size: 5536
AclI AA'CG_TT
Cuts at: 1563 3663 4036 1563
Size: 2100 373 3063
Fragments arranged by size:
3063 2100 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3599 4983 5251 1632
Size: 1967 1384 268 1917
Fragments arranged by size:
1967 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5339
AflIII A'CryG_T
Cuts at: 1203 4435 5412 1203
Size: 3232 977 1327
Fragments arranged by size:
3232 1327 977
AgeI A'CCGG_T
Cuts at: 2782 4367 2782
Size: 1585 3951
AhdI GACnn_n'nnGTC
Cuts at: 4265 4265
Size: 5536
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5536
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2837 1184
Size: 1653 3883
AlwNI CAG_nnn'CTG
Cuts at: 2143 3279 4851 2143
Size: 1136 1572 2828
Fragments arranged by size:
2828 1572 1136
ApaLI G'TGCA_C
Cuts at: 3593 4749 3593
Size: 1156 4380
ApoI r'AATT_y
Cuts at: 1493 2201 2845 3071 3223 5332 1493
Size: 708 644 226 152 2109 1697
Fragments arranged by size:
2109 1697 708 644 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4090 160
Size: 872 3058 1606
Fragments arranged by size:
3058 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5536
AvaI C'yCGr_G
Cuts at: 1870 1943 2861 1870
Size: 73 918 4545
Fragments arranged by size:
4545 918 73
AvrII C'CTAG_G
Cuts at: 2263 2263
Size: 5536
BamHI G'GATC_C
Cuts at: 2613 2866 3233 2613
Size: 253 367 4916
Fragments arranged by size:
4916 367 253
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2288 2857 4312 618
Size: 325 217 627 501 569 1455 1842
Fragments arranged by size:
1842 1455 627 569 501 325 217
BanII G_rGCy'C
Cuts at: 1391 2439 2587 2855 1391
Size: 1048 148 268 4072
Fragments arranged by size:
4072 1048 268 148
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4565
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3727 3761 1903
Size: 34 1790 34 3678
Fragments arranged by size:
3678 1790 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3428 4638 3428
Size: 1210 4326
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5536
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2448 4147 136
Size: 107 122 71 631 605 776 1699 1525
Fragments arranged by size:
1699 1525 776 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5531 5531
Size: 5536
BlpI GC'TnA_GC
Cuts at: 2953 2953
Size: 5536
Bme1580I G_kGCm'C
Cuts at: 1790 2291 3597 4753 1790
Size: 501 1306 1156 2573
Fragments arranged by size:
2573 1306 1156 501
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5536
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2438 3267 4225
Size: 416 53 83 399 24 1021 829 958
Cuts at: 4225 442
Size: 1753
Fragments arranged by size:
1753 1021 958 829 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5536
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2736 4196 1239
Size: 1497 1460 2579
Fragments arranged by size:
2579 1497 1460
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3661 4526 4824 3661
Size: 865 298 4373
Fragments arranged by size:
4373 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2501 4199 882
Size: 617 1002 1698 2219
Fragments arranged by size:
2219 1698 1002 617
BsaAI yAC'GTr
Cuts at: 493 1204 3327 493
Size: 711 2123 2702
Fragments arranged by size:
2702 2123 711
BsaBI GATnn'nnATC
Cuts at: 2600 5530 2600
Size: 2930 2606
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3343 3725 4360 275
Size: 53 83 186 936 1810 382 635 1451
Fragments arranged by size:
1810 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2782 3969 4367 4641 4788 5199 2782
Size: 1187 398 274 147 411 3119
Fragments arranged by size:
3119 1187 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2734 2764 1178
Size: 30 165 30 1331 30 3950
Fragments arranged by size:
3950 1331 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5536
BseYI C'CCAG_C
Cuts at: 4739 5235 4739
Size: 496 5040
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2140 2296 2351 2777 2843 1163
Size: 573 404 156 55 426 66 3856
Fragments arranged by size:
3856 573 426 404 156 66 55
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2986 3747 4775 1540
Size: 275 186 985 761 1028 2301
Fragments arranged by size:
2301 1028 985 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3597 3682 4753 3597
Size: 85 1071 4380
Fragments arranged by size:
4380 1071 85
BsmI GAATG_Cn'
Cuts at: 2183 3047 3140 2183
Size: 864 93 4579
Fragments arranged by size:
4579 864 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2291 2439 2587 2855 3597 3682 4753
Size: 399 501 148 148 268 742 85 1071
Cuts at: 4753 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 501 399 268 148 148
85
BspEI T'CCGG_A
Cuts at: 5199 5199
Size: 5536
BspHI T'CATG_A
Cuts at: 3425 5155 3425
Size: 1730 3806
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2891 844
Size: 2047 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2373 3423 1394
Size: 429 550 1050 3507
Fragments arranged by size:
3507 1050 550 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4031 4205 65
Size: 1183 2783 174 1396
Fragments arranged by size:
2783 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2782 4180 4367 1700
Size: 110 972 1398 187 2869
Fragments arranged by size:
2869 1398 972 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5536
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5536
BssSI C'ACGA_G
Cuts at: 3596 4608 3596
Size: 1012 4524
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5536
BstBI TT'CG_AA
Cuts at: 2478 4340 2478
Size: 1862 3674
BstEII G'GTnAC_C
Cuts at: 1184 2785 1184
Size: 1601 3935
BstXI CCAn_nnnn'nTGG
Cuts at: 2518 2632 2798 2518
Size: 114 166 5256
Fragments arranged by size:
5256 166 114
BstYI r'GATC_y
Cuts at: 1972 2613 2866 3233 3618 3635 5087 5173 5185
Size: 641 253 367 385 17 1452 86 12
Cuts at: 5185 5531 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 641 385 367 346 253 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4045
Fragments arranged by size:
4045 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4797
Fragments arranged by size:
4797 683 56
BtsI GCAGTG_nn'
Cuts at: 2030 2073 2230 2742 3052 3846 3866 2030
Size: 43 157 512 310 794 20 3700
Fragments arranged by size:
3700 794 512 310 157 43 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5536
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5501
DraI TTT'AAA
Cuts at: 2840 2840
Size: 5536
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4543 809
Size: 507 3227 1802
Fragments arranged by size:
3227 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2658 2983 3872
Size: 54 1425 50 275 18 828 325 889
Cuts at: 3872 5501 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 828 325 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2983 1537
Size: 275 1171 4090
Fragments arranged by size:
4090 1171 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3466 1353
Size: 2113 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4123 4497 4643 387
Size: 1064 138 2534 374 146 1280
Fragments arranged by size:
2534 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2736 3599 4196 4983 5251 1239
Size: 393 1104 863 597 787 268 1524
Fragments arranged by size:
1524 1104 863 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2680 2680
Size: 5536
EcoRI G'AATT_C
Cuts at: 2201 2845 3223 2201
Size: 644 378 4514
Fragments arranged by size:
4514 644 378
FspI TGC'GCA
Cuts at: 4042 4042
Size: 5536
HaeII r_GCGC'y
Cuts at: 1678 4683 1678
Size: 3005 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4177 4209 4251 4283 1373
Size: 32 135 32 2605 32 42 32 2626
Fragments arranged by size:
2626 2605 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2880 3126 669
Size: 988 281 942 246 3079
Fragments arranged by size:
3079 988 942 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5536
HpaI GTT'AAC
Cuts at: 3126 3126
Size: 5536
KpnI G_GTAC'C
Cuts at: 2861 2861
Size: 5536
MfeI C'AATT_G
Cuts at: 3135 3135
Size: 5536
MluI A'CGCG_T
Cuts at: 5412 5412
Size: 5536
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2763 4650 4834 1305
Size: 1458 1887 184 2007
Fragments arranged by size:
2007 1887 1458 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2660 10
Size: 54 1425 343 828 2886
Fragments arranged by size:
2886 1425 828 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2819 3494 3853 4012 518
Size: 648 1653 675 359 159 2042
Fragments arranged by size:
2042 1653 675 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2054 2952 3629 4777 5022 1606
Size: 448 898 677 1148 245 2120
Fragments arranged by size:
2120 1148 898 677 448 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4045
Fragments arranged by size:
4045 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5536
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5536
NotI GC'GGCC_GC
Cuts at: 2983 2983
Size: 5536
NspI r_CATG'y
Cuts at: 1132 1229 2082 2894 4439 1132
Size: 97 853 812 1545 2229
Fragments arranged by size:
2229 1545 853 812 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5536
PciI A'CATG_T
Cuts at: 4435 4435
Size: 5536
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5536
PmeI GTTT'AAAC
Cuts at: 2840 2840
Size: 5536
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3605 3637 5118 5150 3605
Size: 32 1481 32 3991
Fragments arranged by size:
3991 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2054 1613
Size: 441 5095
PsiI TTA'TAA
Cuts at: 3106 3106
Size: 5536
PstI C_TGCA'G
Cuts at: 830 2025 2888 830
Size: 1195 863 3478
Fragments arranged by size:
3478 1195 863
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5536
SalI G'TCGA_C
Cuts at: 1936 2878 1936
Size: 942 4594
SbfI CC_TGCA'GG
Cuts at: 2888 2888
Size: 5536
ScaI AGT'ACT
Cuts at: 3784 5496 3784
Size: 1712 3824
SexAI A'CCwGG_T
Cuts at: 2517 2517
Size: 5536
SfcI C'TryA_G
Cuts at: 826 1045 2021 2409 2884 4019 4700 4891 826
Size: 219 976 388 475 1135 681 191 1471
Fragments arranged by size:
1471 1135 976 681 475 388 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5536
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5536
SmaI CCC'GGG
Cuts at: 1872 2863 1872
Size: 991 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3640 4541 4803 820
Size: 197 926 1697 901 262 1553
Fragments arranged by size:
1697 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3327 493
Size: 2834 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5536
SphI G_CATG'C
Cuts at: 1132 2082 2894 1132
Size: 950 812 3774
Fragments arranged by size:
3774 950 812
SspI AAT'ATT
Cuts at: 5 52 3460 5
Size: 47 3408 2081
Fragments arranged by size:
3408 2081 47
StuI AGG'CCT
Cuts at: 1517 2348 1517
Size: 831 4705
StyI C'CwwG_G
Cuts at: 513 1065 2004 2263 2492 2975 513
Size: 552 939 259 229 483 3074
Fragments arranged by size:
3074 939 552 483 259 229
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2086 2804 3563 3580 3733 3918 4363
Size: 1255 506 718 759 17 153 185 445
Cuts at: 4363 325
Size: 1498
Fragments arranged by size:
1498 1255 759 718 506 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3782 5494 96
Size: 274 80 33 51 1443 1805 1712 138
Fragments arranged by size:
1805 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2488 2520 2734 2766 5496 5528 2488
Size: 32 214 32 2730 32 2496
Fragments arranged by size:
2730 2496 214 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5536
XbaI T'CTAG_A
Cuts at: 2872 2872
Size: 5536
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5536
XmaI C'CCGG_G
Cuts at: 1870 2861 1870
Size: 991 4545
XmnI GAAnn'nnTTC
Cuts at: 2181 3665 2181
Size: 1484 4052
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3344 276
Size: 53 83 186 936 1810 2468
Fragments arranged by size:
2468 1810 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI XcmI