Restriction map for
pFN21AE1399
(Circular) (Six-base) MAPSORT of: pFN21AE1399.seq Check: 9835 from: 1 to: 10252
pFN21AE1399 10252 bp
With 182 enzymes: SgfI *
February 20, 2021 22:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2961 4093 6599 2961
Size: 1132 2506 6614
Fragments arranged by size:
6614 2506 1132
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6828 8062 278
Size: 53 83 186 936 5292 1234 2468
Fragments arranged by size:
5292 2468 1234 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4752 5004 7595 1937
Size: 2815 252 2591 4594
Fragments arranged by size:
4594 2815 2591 252
Acc65I G'GTAC_C
Cuts at: 7573 7573
Size: 10252
AclI AA'CG_TT
Cuts at: 1563 6870 8379 8752 1563
Size: 5307 1509 373 3063
Fragments arranged by size:
5307 3063 1509 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2516 2765 4640 4843 4914 8315 9699 9967
Size: 884 249 1875 203 71 3401 1384 268
Cuts at: 9967 1632
Size: 1917
Fragments arranged by size:
3401 1917 1875 1384 884 268 249 203
71
AfeI AGC'GCT
Cuts at: 3292 5416 3292
Size: 2124 8128
AflII C'TTAA_G
Cuts at: 820 1017 4145 820
Size: 197 3128 6927
Fragments arranged by size:
6927 3128 197
AflIII A'CryG_T
Cuts at: 1203 5916 9151 10128 1203
Size: 4713 3235 977 1327
Fragments arranged by size:
4713 3235 1327 977
AgeI A'CCGG_T
Cuts at: 9083 9083
Size: 10252
AhdI GACnn_n'nnGTC
Cuts at: 8981 8981
Size: 10252
AleI CACnn'nnGTG
Cuts at: 1166 2174 3651 6110 1166
Size: 1008 1477 2459 5308
Fragments arranged by size:
5308 2459 1477 1008
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 10252
AlwNI CAG_nnn'CTG
Cuts at: 2944 3352 3861 3997 4603 6293 7543 7995 9567
Size: 408 509 136 606 1690 1250 452 1572
Cuts at: 9567 2944
Size: 3629
Fragments arranged by size:
3629 1690 1572 1250 606 509 452 408
136
ApaI G_GGCC'C
Cuts at: 3417 3558 5550 3417
Size: 141 1992 8119
Fragments arranged by size:
8119 1992 141
ApaLI G'TGCA_C
Cuts at: 5951 8309 9465 5951
Size: 2358 1156 6738
Fragments arranged by size:
6738 2358 1156
ApoI r'AATT_y
Cuts at: 1493 3959 4783 5524 6251 7561 7787 7939 10048
Size: 2466 824 741 727 1310 226 152 2109
Cuts at: 10048 1493
Size: 1697
Fragments arranged by size:
2466 2109 1697 1310 824 741 727 226
152
AseI AT'TA_AT
Cuts at: 160 1032 8806 160
Size: 872 7774 1606
Fragments arranged by size:
7774 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10252
AvaI C'yCGr_G
Cuts at: 1870 1943 2721 2992 3246 3374 3410 3550 3584
Size: 73 778 271 254 128 36 140 34
Cuts at: 3584 4319 4524 5072 5384 5549 5597 5811 6098
Size: 735 205 548 312 165 48 214 287
Cuts at: 6098 6515 7497 7577 1870
Size: 417 982 80 4545
Fragments arranged by size:
4545 982 778 735 548 417 312 287
271 254 214 205 165 140 128 80
73 48 36 34
BamHI G'GATC_C
Cuts at: 2262 2566 7582 7949 2262
Size: 304 5016 367 4565
Fragments arranged by size:
5016 4565 367 304
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2141 3515 4222 5483 6046
Size: 325 217 627 354 1374 707 1261 563
Cuts at: 6046 7052 7573 9028 618
Size: 1006 521 1455 1842
Fragments arranged by size:
1842 1455 1374 1261 1006 707 627 563
521 354 325 217
BanII G_rGCy'C
Cuts at: 1391 2671 3417 3558 3833 4281 4433 4499 5463
Size: 1280 746 141 275 448 152 66 964
Cuts at: 5463 5550 6105 6143 7571 1391
Size: 87 555 38 1428 4072
Fragments arranged by size:
4072 1428 1280 964 746 555 448 275
152 141 87 66 38
BbeI G_GCGC'C
Cuts at: 5487 5487
Size: 10252
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2492 2753 3395 3884 4902 5084 6555
Size: 971 593 261 642 489 1018 182 1471
Cuts at: 6555 928
Size: 4625
Fragments arranged by size:
4625 1471 1018 971 642 593 489 261
182
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5149 5182 5183 5216 5477
Size: 34 50 34 3128 33 1 33 261
Cuts at: 5477 5511 6109 6143 8443 8477 1903
Size: 34 598 34 2300 34 3678
Fragments arranged by size:
3678 3128 2300 598 261 50 34 34
34 34 34 33 33 1
BciVI GTATCCnnnnn_n'
Cuts at: 8144 9354 8144
Size: 1210 9042
BclI T'GATC_A
Cuts at: 1548 1548
Size: 10252
BfrBI ATG'CAT
Cuts at: 2217 2505 2217
Size: 288 9964
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3487 3589 5026
Size: 107 122 71 631 605 1815 102 1437
Cuts at: 5026 5723 8863 136
Size: 697 3140 1525
Fragments arranged by size:
3140 1815 1525 1437 697 631 605 122
107 102 71
BglII A'GATC_T
Cuts at: 6084 10247 6084
Size: 4163 6089
BlpI GC'TnA_GC
Cuts at: 2619 5418 7669 2619
Size: 2799 2251 5202
Fragments arranged by size:
5202 2799 2251
Bme1580I G_kGCm'C
Cuts at: 1790 2934 2983 3183 3411 3417 3558 3585 4225
Size: 1144 49 200 228 6 141 27 640
Cuts at: 4225 4689 5550 5571 5955 6117 6772 7126 7509
Size: 464 861 21 384 162 655 354 383
Cuts at: 7509 8313 9469 1790
Size: 804 1156 2573
Fragments arranged by size:
2573 1156 1144 861 804 655 640 464
384 383 354 228 200 162 141 49
27 21 6
BmgBI CAC'GTC
Cuts at: 1318 5082 5532 5919 7121 1318
Size: 3764 450 387 1202 4449
Fragments arranged by size:
4449 3764 1202 450 387
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3310 6632 7983
Size: 416 53 83 399 24 1893 3322 1351
Cuts at: 7983 8941 442
Size: 958 1753
Fragments arranged by size:
3322 1893 1753 1351 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10252
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2432 2966 4019 4292 6346 6779 8912 1239
Size: 1193 534 1053 273 2054 433 2133 2579
Fragments arranged by size:
2579 2133 2054 1193 1053 534 433 273
Bpu10I CC'TnA_GC
Cuts at: 2611 6648 2611
Size: 4037 6215
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 8377 9242 9540 8377
Size: 865 298 9089
Fragments arranged by size:
9089 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2090 2582 2591 5846 5862 7144 7157
Size: 617 591 492 9 3255 16 1282 13
Cuts at: 7157 8915 882
Size: 1758 2219
Fragments arranged by size:
3255 2219 1758 1282 617 591 492 16
13 9
BsaAI yAC'GTr
Cuts at: 493 1204 2935 3406 8043 493
Size: 711 1731 471 4637 2702
Fragments arranged by size:
4637 2702 1731 711 471
BsaBI GATnn'nnATC
Cuts at: 5906 10246 5906
Size: 4340 5912
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4478 5484 5964 6456
Size: 53 83 186 936 2945 1006 480 492
Cuts at: 6456 6825 8059 8441 9076 275
Size: 369 1234 382 635 1451
Fragments arranged by size:
2945 1451 1234 1006 936 635 492 480
382 369 186 83 53
BsaWI w'CCGG_w
Cuts at: 2591 4299 5871 8685 9083 9357 9504 9915 2591
Size: 1708 1572 2814 398 274 147 411 2928
Fragments arranged by size:
2928 2814 1708 1572 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3740 3770 4865 4895 5831
Size: 30 165 30 2337 30 1095 30 936
Cuts at: 5831 5861 6567 6597 7183 7213 1178
Size: 30 706 30 586 30 4217
Fragments arranged by size:
4217 2337 1095 936 706 586 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2168 2372 3761 3887 4289 4337 4599 5402
Size: 982 204 1389 126 402 48 262 803
Cuts at: 5402 6171 6231 6494 1186
Size: 769 60 263 4944
Fragments arranged by size:
4944 1389 982 803 769 402 263 262
204 126 60 48
BseYI C'CCAG_C
Cuts at: 3345 3365 3445 3982 4406 5510 5560 5639 5768
Size: 20 80 537 424 1104 50 79 129
Cuts at: 5768 5922 6771 7059 7273 9455 9951 3345
Size: 154 849 288 214 2182 496 3646
Fragments arranged by size:
3646 2182 1104 849 537 496 424 288
214 154 129 80 79 50 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2843 2957 6577 1163
Size: 573 1107 114 3620 4838
Fragments arranged by size:
4838 3620 1107 573 114
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5269 7702 8463 9491 1540
Size: 275 186 3268 2433 761 1028 2301
Fragments arranged by size:
3268 2433 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2671 2952 4281 5087 5500 5955 7004 7025 8313
Size: 281 1329 806 413 455 1049 21 1288
Cuts at: 8313 8398 9469 2671
Size: 85 1071 3454
Fragments arranged by size:
3454 1329 1288 1071 1049 806 455 413
281 85 21
BsmI GAATG_Cn'
Cuts at: 2217 2992 4918 7763 7856 2217
Size: 775 1926 2845 93 4613
Fragments arranged by size:
4613 2845 1926 775 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5828 6512 5828
Size: 684 9568
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2671 2934 2952 2983 3183 3411 3417
Size: 399 881 263 18 31 200 228 6
Cuts at: 3417 3558 3585 3833 4225 4281 4433 4499 4689
Size: 141 27 248 392 56 152 66 190
Cuts at: 4689 5087 5463 5500 5550 5571 5955 6105 6117
Size: 398 376 37 50 21 384 150 12
Cuts at: 6117 6143 6772 7004 7025 7126 7509 7571 8313
Size: 26 629 232 21 101 383 62 742
Cuts at: 8313 8398 9469 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 881 742 629 399 398 392
384 383 376 263 248 232 228 200
190 152 150 141 101 85 66 62
56 50 37 31 27 26 21 21
18 12 6
BspEI T'CCGG_A
Cuts at: 4299 9915 4299
Size: 5616 4636
BspHI T'CATG_A
Cuts at: 6996 8141 9871 6996
Size: 1145 1730 7377
Fragments arranged by size:
7377 1730 1145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2208 2913 2961 4093 6599 7470 7607 844
Size: 1364 705 48 1132 2506 871 137 3489
Fragments arranged by size:
3489 2506 1364 1132 871 705 137 48
BsrBI CCG'CTC
Cuts at: 1394 1823 2362 2618 4594 6746 7211 8139 1394
Size: 429 539 256 1976 2152 465 928 3507
Fragments arranged by size:
3507 2152 1976 928 539 465 429 256
BsrDI GCAATG_nn'
Cuts at: 65 1248 2335 6233 6688 8747 8921 65
Size: 1183 1087 3898 455 2059 174 1396
Fragments arranged by size:
3898 2059 1396 1183 1087 455 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2320 3213 3423 3973 4413 4916 5591
Size: 110 510 893 210 550 440 503 675
Cuts at: 5591 5780 7080 8896 9083 1700
Size: 189 1300 1816 187 2869
Fragments arranged by size:
2869 1816 1300 893 675 550 510 503
440 210 189 187 110
BsrGI T'GTAC_A
Cuts at: 96 2270 96
Size: 2174 8078
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10252
BssSI C'ACGA_G
Cuts at: 2903 4280 6704 8312 9324 2903
Size: 1377 2424 1608 1012 3831
Fragments arranged by size:
3831 2424 1608 1377 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3361 4416 4684 794
Size: 2567 1055 268 6362
Fragments arranged by size:
6362 2567 1055 268
BstBI TT'CG_AA
Cuts at: 9056 9056
Size: 10252
BstEII G'GTnAC_C
Cuts at: 1184 4159 5794 7030 1184
Size: 2975 1635 1236 4406
Fragments arranged by size:
4406 2975 1635 1236
BstXI CCAn_nnnn'nTGG
Cuts at: 2551 3235 4171 5323 5974 2551
Size: 684 936 1152 651 6829
Fragments arranged by size:
6829 1152 936 684 651
BstYI r'GATC_y
Cuts at: 1972 2262 2566 2907 3636 6084 7255 7582 7949
Size: 290 304 341 729 2448 1171 327 367
Cuts at: 7949 8334 8351 9803 9889 9901 10247 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
2448 1977 1452 1171 729 385 367 346
341 327 304 290 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5822 7108 5822
Size: 1286 8966
BtgI C'CryG_G
Cuts at: 513 1065 2064 2388 3315 3649 3709 3825 4005
Size: 552 999 324 927 334 60 116 180
Cuts at: 4005 4824 5958 6144 6198 513
Size: 819 1134 186 54 4567
Fragments arranged by size:
4567 1134 999 927 819 552 334 324
186 180 116 60 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2507 3417 5233 5895 487
Size: 683 56 1281 910 1816 662 4844
Fragments arranged by size:
4844 1816 1281 910 683 662 56
BtsI GCAGTG_nn'
Cuts at: 2121 5571 6772 7768 8562 8582 2121
Size: 3450 1201 996 794 20 3791
Fragments arranged by size:
3791 3450 1201 996 794 20
ClaI AT'CG_AT
Cuts at: 1554 7440 7482 1554
Size: 5886 42 4324
Fragments arranged by size:
5886 4324 42
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2644 2679 7116 7151 529
Size: 35 2080 35 4437 35 3630
Fragments arranged by size:
4437 3630 2080 35 35 35
DraI TTT'AAA
Cuts at: 7556 7556
Size: 10252
DraIII CAC_nnn'GTG
Cuts at: 2244 2877 5959 2244
Size: 633 3082 6537
Fragments arranged by size:
6537 3082 633
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3305 9259 809
Size: 507 1989 5954 1802
Fragments arranged by size:
5954 1989 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2322 2469 3114
Size: 54 1425 50 275 18 492 147 645
Cuts at: 3114 3237 3318 3712 4093 4827 6783 7377 7699
Size: 123 81 394 381 734 1956 594 322
Cuts at: 7699 8588 10217 8
Size: 889 1629 43
Fragments arranged by size:
1956 1629 1425 889 734 645 594 492
394 381 322 275 147 123 81 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7699 1537
Size: 275 5887 4090
Fragments arranged by size:
5887 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2088 3599 4584 5988 6144 8182 1353
Size: 735 1511 985 1404 156 2038 3423
Fragments arranged by size:
3423 2038 1511 1404 985 735 156
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3081 3749 5044 6955 8839 9213
Size: 1064 138 1492 668 1295 1911 1884 374
Cuts at: 9213 9359 387
Size: 146 1280
Fragments arranged by size:
1911 1884 1492 1295 1280 1064 668 374
146 138
EcoICRI GAG'CTC
Cuts at: 2669 4279 2669
Size: 1610 8642
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2432 2516 2765 2966 4019 4292 4640
Size: 393 800 84 249 201 1053 273 348
Cuts at: 4640 4843 4914 6346 6779 8315 8912 9699 9967
Size: 203 71 1432 433 1536 597 787 268
Cuts at: 9967 1239
Size: 1524
Fragments arranged by size:
1536 1524 1432 1053 800 787 597 433
393 348 273 268 249 203 201 84
71
EcoNI CCTnn'n_nnAGG
Cuts at: 2354 7466 2354
Size: 5112 5140
EcoO109I rG'GnC_Cy
Cuts at: 3193 3250 3414 3554 5179 5546 5702 5716 5816
Size: 57 164 140 1625 367 156 14 100
Cuts at: 5816 6804 7104 7474 3193
Size: 988 300 370 5971
Fragments arranged by size:
5971 1625 988 370 367 300 164 156
140 100 57 14
EcoRI G'AATT_C
Cuts at: 3959 4783 5524 7561 7939 3959
Size: 824 741 2037 378 6272
Fragments arranged by size:
6272 2037 824 741 378
EcoRV GAT'ATC
Cuts at: 6271 6271
Size: 10252
FspI TGC'GCA
Cuts at: 8758 8758
Size: 10252
HaeII r_GCGC'y
Cuts at: 1678 3294 3708 5418 5487 9399 1678
Size: 1616 414 1710 69 3912 2531
Fragments arranged by size:
3912 2531 1710 1616 414 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3518 3550 4153 4185 4277
Size: 32 135 32 1946 32 603 32 92
Cuts at: 4277 4309 4427 4459 4865 4897 5411 5443 5627
Size: 32 118 32 406 32 514 32 184
Cuts at: 5627 5659 8893 8925 8967 8999 1373
Size: 32 3234 32 42 32 2626
Fragments arranged by size:
3234 2626 1946 603 514 406 184 135
118 92 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4069 6628 7596 7842 669
Size: 988 281 2131 2559 968 246 3079
Fragments arranged by size:
3079 2559 2131 988 968 281 246
HindIII A'AGCT_T
Cuts at: 748 3806 6448 748
Size: 3058 2642 4552
Fragments arranged by size:
4552 3058 2642
HpaI GTT'AAC
Cuts at: 4069 7842 4069
Size: 3773 6479
KasI G'GCGC_C
Cuts at: 5483 5483
Size: 10252
KpnI G_GTAC'C
Cuts at: 7577 7577
Size: 10252
MfeI C'AATT_G
Cuts at: 7851 7851
Size: 10252
MluI A'CGCG_T
Cuts at: 10128 10128
Size: 10252
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2052 2079 2806 3231 3288 4842 4889 5079
Size: 747 27 727 425 57 1554 47 190
Cuts at: 5079 9366 9550 1305
Size: 4287 184 2007
Fragments arranged by size:
4287 2007 1554 747 727 425 190 184
57 47 27
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2471 3116 4829 6785 10
Size: 54 1425 343 639 645 1713 1956 3477
Fragments arranged by size:
3477 1956 1713 1425 645 639 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2174 2546 3651 4370 4835 5108 6110
Size: 648 1008 372 1105 719 465 273 1002
Cuts at: 6110 8210 8569 8728 518
Size: 2100 359 159 2042
Fragments arranged by size:
2100 2042 1105 1008 1002 719 648 465
372 359 273 159
MspA1I CmG'CkG
Cuts at: 1606 2260 2623 2806 3039 3349 3651 3711 4402
Size: 654 363 183 233 310 302 60 691
Cuts at: 4402 5136 5200 5425 5521 5583 5650 6092 7277
Size: 734 64 225 96 62 67 442 1185
Cuts at: 7277 7540 7668 8345 9493 9738 1606
Size: 263 128 677 1148 245 2120
Fragments arranged by size:
2120 1185 1148 734 691 677 654 442
363 310 302 263 245 233 225 183
128 96 67 64 62 60
NaeI GCC'GGC
Cuts at: 2322 3215 4918 5593 5782 7082 2322
Size: 893 1703 675 189 1300 5492
Fragments arranged by size:
5492 1703 1300 893 675 189
NarI GG'CG_CC
Cuts at: 5484 5484
Size: 10252
NcoI C'CATG_G
Cuts at: 513 1065 6144 513
Size: 552 5079 4621
Fragments arranged by size:
5079 4621 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10252
NgoMIV G'CCGG_C
Cuts at: 2320 3213 4916 5591 5780 7080 2320
Size: 893 1703 675 189 1300 5492
Fragments arranged by size:
5492 1703 1300 893 675 189
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10252
NotI GC'GGCC_GC
Cuts at: 7699 7699
Size: 10252
NsiI A_TGCA'T
Cuts at: 2219 2507 2219
Size: 288 9964
NspI r_CATG'y
Cuts at: 1132 1229 3494 5899 7610 9155 1132
Size: 97 2265 2405 1711 1545 2229
Fragments arranged by size:
2405 2265 2229 1711 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3765 5454 5542 1348
Size: 2417 1689 88 6058
Fragments arranged by size:
6058 2417 1689 88
PciI A'CATG_T
Cuts at: 9151 9151
Size: 10252
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2244 5476 6816 1108
Size: 1136 3232 1340 4544
Fragments arranged by size:
4544 3232 1340 1136
PfoI T'CCnGG_A
Cuts at: 2349 2349
Size: 10252
PmeI GTTT'AAAC
Cuts at: 7556 7556
Size: 10252
PmlI CAC'GTG
Cuts at: 2935 2935
Size: 10252
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8321 8353 9834 9866 8321
Size: 32 1481 32 8707
Fragments arranged by size:
8707 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3250 5702 5816 6804 7104 7474 3250
Size: 2452 114 988 300 370 6028
Fragments arranged by size:
6028 2452 988 370 300 114
PshAI GACnn'nnGTC
Cuts at: 1613 3884 1613
Size: 2271 7981
PsiI TTA'TAA
Cuts at: 2781 7822 2781
Size: 5041 5211
PspOMI G'GGCC_C
Cuts at: 3413 3554 5546 3413
Size: 141 1992 8119
Fragments arranged by size:
8119 1992 141
PspXI vC'TCGA_Gb
Cuts at: 4524 7497 4524
Size: 2973 7279
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4433 4465 4433
Size: 32 10220
PstI C_TGCA'G
Cuts at: 830 2205 2924 3357 3894 5991 6385 7077 7467
Size: 1375 719 433 537 2097 394 692 390
Cuts at: 7467 7604 830
Size: 137 3478
Fragments arranged by size:
3478 2097 1375 719 692 537 433 394
390 137
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10252
PvuII CAG'CTG
Cuts at: 2623 3039 3349 5200 5425 7540 2623
Size: 416 310 1851 225 2115 5335
Fragments arranged by size:
5335 2115 1851 416 310 225
RsrII CG'GwC_CG
Cuts at: 4297 4297
Size: 10252
SacI G_AGCT'C
Cuts at: 2671 4281 2671
Size: 1610 8642
SacII CC_GC'GG
Cuts at: 3652 3712 3652
Size: 60 10192
SalI G'TCGA_C
Cuts at: 1936 7594 1936
Size: 5658 4594
SanDI GG'GwC_CC
Cuts at: 5702 5816 5702
Size: 114 10138
SapI GCTCTTCn'nnn_
Cuts at: 3599 4584 3599
Size: 985 9267
SbfI CC_TGCA'GG
Cuts at: 2924 5991 7604 2924
Size: 3067 1613 5572
Fragments arranged by size:
5572 3067 1613
ScaI AGT'ACT
Cuts at: 8500 10212 8500
Size: 1712 8540
SexAI A'CCwGG_T
Cuts at: 4137 4811 4137
Size: 674 9578
SfcI C'TryA_G
Cuts at: 826 1045 2201 2920 3353 3571 3890 3989 4903
Size: 219 1156 719 433 218 319 99 914
Cuts at: 4903 5987 6381 6896 7073 7463 7600 8735 9416
Size: 1084 394 515 177 390 137 1135 681
Cuts at: 9416 9607 826
Size: 191 1471
Fragments arranged by size:
1471 1156 1135 1084 914 719 681 515
433 394 390 319 219 218 191 177
137 99
SfiI GGCCn_nnn'nGGCC
Cuts at: 5723 5723
Size: 10252
SfoI GGC'GCC
Cuts at: 5485 5485
Size: 10252
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10252
SgrAI Cr'CCGG_yG
Cuts at: 1700 3213 1700
Size: 1513 8739
SmaI CCC'GGG
Cuts at: 1872 3248 3412 3552 3586 5599 5813 7579 1872
Size: 1376 164 140 34 2013 214 1766 4545
Fragments arranged by size:
4545 2013 1766 1376 214 164 140 34
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4145 4524 6515 7497 8356 9257
Size: 197 926 2202 379 1991 982 859 901
Cuts at: 9257 9519 820
Size: 262 1553
Fragments arranged by size:
2202 1991 1553 982 926 901 859 379
262 197
SnaBI TAC'GTA
Cuts at: 493 8043 493
Size: 7550 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10252
SphI G_CATG'C
Cuts at: 1132 7610 1132
Size: 6478 3774
SrfI GCCC'GGGC
Cuts at: 3412 3412
Size: 10252
SspI AAT'ATT
Cuts at: 5 52 8176 5
Size: 47 8124 2081
Fragments arranged by size:
8124 2081 47
StuI AGG'CCT
Cuts at: 1517 2562 2918 7421 1517
Size: 1045 356 4503 4348
Fragments arranged by size:
4503 4348 1045 356
StyI C'CwwG_G
Cuts at: 513 1065 4459 4794 4884 5721 6144 6786 6800
Size: 552 3394 335 90 837 423 642 14
Cuts at: 6800 7691 513
Size: 891 3074
Fragments arranged by size:
3394 3074 891 837 642 552 423 335
90 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2381 2571 3964 4285 5468 5628 5691
Size: 1255 801 190 1393 321 1183 160 63
Cuts at: 5691 5870 7394 8279 8296 8449 8634 9079 325
Size: 179 1524 885 17 153 185 445 1498
Fragments arranged by size:
1524 1498 1393 1255 1183 885 801 445
321 190 185 179 160 153 63 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2270 4854 8498
Size: 274 80 33 51 1443 293 2584 3644
Cuts at: 8498 10210 96
Size: 1712 138
Fragments arranged by size:
3644 2584 1712 1443 293 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3740 3772 3963 3995 4455 4487 6567 6599 7183
Size: 32 191 32 460 32 2080 32 584
Cuts at: 7183 7215 10212 10244 3740
Size: 32 2997 32 3748
Fragments arranged by size:
3748 2997 2080 584 460 191 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3659 6458 1316
Size: 2343 2799 5110
Fragments arranged by size:
5110 2799 2343
XbaI T'CTAG_A
Cuts at: 7588 7588
Size: 10252
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5477 5647 6133 7247 5477
Size: 170 486 1114 8482
Fragments arranged by size:
8482 1114 486 170
XhoI C'TCGA_G
Cuts at: 1943 4524 6515 7497 1943
Size: 2581 1991 982 4698
Fragments arranged by size:
4698 2581 1991 982
XmaI C'CCGG_G
Cuts at: 1870 3246 3410 3550 3584 5597 5811 7577 1870
Size: 1376 164 140 34 2013 214 1766 4545
Fragments arranged by size:
4545 2013 1766 1376 214 164 140 34
XmnI GAAnn'nnTTC
Cuts at: 3809 8381 3809
Size: 4572 5680
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6826 8060 276
Size: 53 83 186 936 5292 1234 2468
Fragments arranged by size:
5292 2468 1234 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BplI BsiWI BstZ17I
FalI FseI FspAI NruI PacI SwaI