Restriction map for
pFN21AE1391
(Circular) (Six-base) MAPSORT of: pFN21AE1391.seq Check: 2093 from: 1 to: 7507
pFN21AE1391 7507 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4610 4610
Size: 7507
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5317 278
Size: 53 83 186 936 3781 2468
Fragments arranged by size:
3781 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3696 4850 1937
Size: 1759 1154 4594
Fragments arranged by size:
4594 1759 1154
Acc65I G'GTAC_C
Cuts at: 4828 4828
Size: 7507
AclI AA'CG_TT
Cuts at: 1563 5634 6007 1563
Size: 4071 373 3063
Fragments arranged by size:
4071 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4039 5570 6954 7222 1632
Size: 2407 1531 1384 268 1917
Fragments arranged by size:
2407 1917 1531 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7310
AflIII A'CryG_T
Cuts at: 1203 3223 6406 7383 1203
Size: 2020 3183 977 1327
Fragments arranged by size:
3183 2020 1327 977
AgeI A'CCGG_T
Cuts at: 6338 6338
Size: 7507
AhdI GACnn_n'nnGTC
Cuts at: 2018 3618 6236 2018
Size: 1600 2618 3289
Fragments arranged by size:
3289 2618 1600
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7507
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3561 1184
Size: 2377 5130
AlwNI CAG_nnn'CTG
Cuts at: 3614 4349 5250 6822 3614
Size: 735 901 1572 4299
Fragments arranged by size:
4299 1572 901 735
ApaLI G'TGCA_C
Cuts at: 3262 5564 6720 3262
Size: 2302 1156 4049
Fragments arranged by size:
4049 2302 1156
ApoI r'AATT_y
Cuts at: 1493 2432 4011 4249 4816 5042 5194 7303 1493
Size: 939 1579 238 567 226 152 2109 1697
Fragments arranged by size:
2109 1697 1579 939 567 238 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6061 160
Size: 872 5029 1606
Fragments arranged by size:
5029 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7507
AvaI C'yCGr_G
Cuts at: 1870 1943 3762 4832 1870
Size: 73 1819 1070 4545
Fragments arranged by size:
4545 1819 1070 73
BamHI G'GATC_C
Cuts at: 2949 4837 5204 2949
Size: 1888 367 5252
Fragments arranged by size:
5252 1888 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3641 4828 6283 618
Size: 325 217 627 1854 1187 1455 1842
Fragments arranged by size:
1854 1842 1455 1187 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2616 2837 3389 4826 1391
Size: 1225 221 552 1437 4072
Fragments arranged by size:
4072 1437 1225 552 221
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3410 3711 3837 4383 928
Size: 971 1511 301 126 546 4052
Fragments arranged by size:
4052 1511 971 546 301 126
BbvCI CC'TCA_GC
Cuts at: 3939 3939
Size: 7507
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5698 5732 1903
Size: 34 3761 34 3678
Fragments arranged by size:
3761 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4037 5399 6609 4037
Size: 1362 1210 4935
Fragments arranged by size:
4935 1362 1210
BclI T'GATC_A
Cuts at: 1548 2049 2077 1548
Size: 501 28 6978
Fragments arranged by size:
6978 501 28
BfrBI ATG'CAT
Cuts at: 2087 2385 4356 2087
Size: 298 1971 5238
Fragments arranged by size:
5238 1971 298
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4026 4206 4521
Size: 107 122 71 631 605 2354 180 315
Cuts at: 4521 6118 136
Size: 1597 1525
Fragments arranged by size:
2354 1597 1525 631 605 315 180 122
107 71
BglII A'GATC_T
Cuts at: 2315 2995 4399 7502 2315
Size: 680 1404 3103 2320
Fragments arranged by size:
3103 2320 1404 680
BlpI GC'TnA_GC
Cuts at: 4924 4924
Size: 7507
Bme1580I G_kGCm'C
Cuts at: 1790 3266 5568 6724 1790
Size: 1476 2302 1156 2573
Fragments arranged by size:
2573 2302 1476 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7507
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2325 4621 5238
Size: 416 53 83 399 24 908 2296 617
Cuts at: 5238 6196 442
Size: 958 1753
Fragments arranged by size:
2296 1753 958 908 617 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7507
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2667 3416 3657 3745 3936 4099 4353 6167
Size: 1428 749 241 88 191 163 254 1814
Cuts at: 6167 1239
Size: 2579
Fragments arranged by size:
2579 1814 1428 749 254 241 191 163
88
Bpu10I CC'TnA_GC
Cuts at: 3939 3939
Size: 7507
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2803 5632 6497 6795 2803
Size: 2829 865 298 3515
Fragments arranged by size:
3515 2829 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6170 882
Size: 617 4671 2219
Fragments arranged by size:
4671 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5298 493
Size: 711 4094 2702
Fragments arranged by size:
4094 2702 711
BsaBI GATnn'nnATC
Cuts at: 7501 7501
Size: 7507
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5314 5696 6331 275
Size: 53 83 186 936 3781 382 635 1451
Fragments arranged by size:
3781 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3969 5940 6338 6612 6759 7170 3969
Size: 1971 398 274 147 411 4306
Fragments arranged by size:
4306 1971 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3376 3406 3718 3748 3990
Size: 30 165 30 1973 30 312 30 242
Cuts at: 3990 4020 4406 4436 1178
Size: 30 386 30 4249
Fragments arranged by size:
4249 1973 386 312 242 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2634 3413 3807 1186
Size: 1448 779 394 4886
Fragments arranged by size:
4886 1448 779 394
BseYI C'CCAG_C
Cuts at: 4220 4327 6710 7206 4220
Size: 107 2383 496 4521
Fragments arranged by size:
4521 2383 496 107
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2984 3522 3820 4151 1163
Size: 573 1248 538 298 331 4519
Fragments arranged by size:
4519 1248 573 538 331 298
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4525 4957 5718 6746 1540
Size: 275 186 2524 432 761 1028 2301
Fragments arranged by size:
2524 2301 1028 761 432 275 186
BsiHKAI G_wGCw'C
Cuts at: 2837 3266 3389 3577 5568 5653 6724 2837
Size: 429 123 188 1991 85 1071 3620
Fragments arranged by size:
3620 1991 1071 429 188 123 85
BsmI GAATG_Cn'
Cuts at: 2047 2385 2480 3773 4103 4358 5018 5111 2047
Size: 338 95 1293 330 255 660 93 4443
Fragments arranged by size:
4443 1293 660 338 330 255 95 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2616 2837 3266 3389 3577 4826 5568
Size: 399 826 221 429 123 188 1249 742
Cuts at: 5568 5653 6724 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1249 1071 826 742 429 399 221
188 123 85
BspEI T'CCGG_A
Cuts at: 3969 7170 3969
Size: 3201 4306
BspHI T'CATG_A
Cuts at: 5396 7126 5396
Size: 1730 5777
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4610 4862 844
Size: 3766 252 3489
Fragments arranged by size:
3766 3489 252
BsrBI CCG'CTC
Cuts at: 1394 1823 5394 1394
Size: 429 3571 3507
Fragments arranged by size:
3571 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2457 2485 2593 6002 6176 65
Size: 1183 1209 28 108 3409 174 1396
Fragments arranged by size:
3409 1396 1209 1183 174 108 28
BsrFI r'CCGG_y
Cuts at: 1700 1810 6151 6338 1700
Size: 110 4341 187 2869
Fragments arranged by size:
4341 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2572 3063 96
Size: 2476 491 4540
Fragments arranged by size:
4540 2476 491
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7507
BssSI C'ACGA_G
Cuts at: 5567 6579 5567
Size: 1012 6495
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7507
BstBI TT'CG_AA
Cuts at: 6311 6311
Size: 7507
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7507
BstYI r'GATC_y
Cuts at: 1972 2315 2949 2995 4346 4399 4837 5204 5589
Size: 343 634 46 1351 53 438 367 385
Cuts at: 5589 5606 7058 7144 7156 7502 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1351 634 438 385 367 346
343 86 53 46 17 12
Bsu36I CC'TnA_GG
Cuts at: 3524 3524
Size: 7507
BtgI C'CryG_G
Cuts at: 513 1065 2004 2270 513
Size: 552 939 266 5750
Fragments arranged by size:
5750 939 552 266
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6768
Fragments arranged by size:
6768 683 56
BtsI GCAGTG_nn'
Cuts at: 3157 3510 5023 5817 5837 3157
Size: 353 1513 794 20 4827
Fragments arranged by size:
4827 1513 794 353 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7507
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7472
DraI TTT'AAA
Cuts at: 4476 4811 4476
Size: 335 7172
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6514 809
Size: 507 5198 1802
Fragments arranged by size:
5198 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2449 2703
Size: 54 1425 50 275 18 177 442 254
Cuts at: 2703 3592 4522 4803 4954 5843 7472 8
Size: 889 930 281 151 889 1629 43
Fragments arranged by size:
1629 1425 930 889 889 442 281 275
254 177 151 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4522 4954 1537
Size: 275 2710 432 4090
Fragments arranged by size:
4090 2710 432 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2406 5437 1353
Size: 1053 3031 3423
Fragments arranged by size:
3423 3031 1053
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6094 6468 6614 387
Size: 1064 138 4505 374 146 1280
Fragments arranged by size:
4505 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2835 3387 2835
Size: 552 6955
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2667 3416 3657 3745 3936 4039 4099
Size: 393 1035 749 241 88 191 103 60
Cuts at: 4099 4353 5570 6167 6954 7222 1239
Size: 254 1217 597 787 268 1524
Fragments arranged by size:
1524 1217 1035 787 749 597 393 268
254 241 191 103 88 60
EcoO109I rG'GnC_Cy
Cuts at: 4074 4074
Size: 7507
EcoRI G'AATT_C
Cuts at: 4249 4816 5194 4249
Size: 567 378 6562
Fragments arranged by size:
6562 567 378
FspI TGC'GCA
Cuts at: 6013 6013
Size: 7507
HaeII r_GCGC'y
Cuts at: 1678 6654 1678
Size: 4976 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2004 2036 2226 2258 2610
Size: 32 135 32 432 32 190 32 352
Cuts at: 2610 2642 2671 2703 3376 3408 3588 3604 3620
Size: 32 29 32 673 32 180 16 16
Cuts at: 3620 3636 3653 3668 3685 3700 4406 4438 6148
Size: 16 17 15 17 15 706 32 1710
Cuts at: 6148 6180 6222 6254 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1710 706 673 432 352 190 180
135 42 32 32 32 32 32 32
32 32 32 32 29 17 17 16
16 16 15 15
HincII GTy'rAC
Cuts at: 669 1657 1938 4851 5097 669
Size: 988 281 2913 246 3079
Fragments arranged by size:
3079 2913 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3531 748
Size: 2783 4724
HpaI GTT'AAC
Cuts at: 5097 5097
Size: 7507
KpnI G_GTAC'C
Cuts at: 4832 4832
Size: 7507
MfeI C'AATT_G
Cuts at: 5106 5106
Size: 7507
MluI A'CGCG_T
Cuts at: 3223 7383 3223
Size: 4160 3347
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3976 4021 4449 4590 6621 6805 1305
Size: 2671 45 428 141 2031 184 2007
Fragments arranged by size:
2671 2031 2007 428 184 141 45
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 5508
Fragments arranged by size:
5508 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4361 5465 5824 5983 518
Size: 648 3195 1104 359 159 2042
Fragments arranged by size:
3195 2042 1104 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2106 2454 4327 4923 5600 6748 6993 1606
Size: 500 348 1873 596 677 1148 245 2120
Fragments arranged by size:
2120 1873 1148 677 596 500 348 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2270 513
Size: 552 939 266 5750
Fragments arranged by size:
5750 939 552 266
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7507
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7507
NotI GC'GGCC_GC
Cuts at: 4954 4954
Size: 7507
NsiI A_TGCA'T
Cuts at: 2089 2387 4358 2089
Size: 298 1971 5238
Fragments arranged by size:
5238 1971 298
NspI r_CATG'y
Cuts at: 1132 1229 4242 4865 6410 1132
Size: 97 3013 623 1545 2229
Fragments arranged by size:
3013 2229 1545 623 97
PasI CC'CwG_GG
Cuts at: 1348 3115 1348
Size: 1767 5740
PciI A'CATG_T
Cuts at: 6406 6406
Size: 7507
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3959 1108
Size: 2851 4656
PfoI T'CCnGG_A
Cuts at: 3722 4253 4789 3722
Size: 531 536 6440
Fragments arranged by size:
6440 536 531
PmeI GTTT'AAAC
Cuts at: 4811 4811
Size: 7507
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5576 5608 7089 7121 5576
Size: 32 1481 32 5962
Fragments arranged by size:
5962 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4074 4074
Size: 7507
PshAI GACnn'nnGTC
Cuts at: 1613 2272 1613
Size: 659 6848
PsiI TTA'TAA
Cuts at: 3681 3747 5077 3681
Size: 66 1330 6111
Fragments arranged by size:
6111 1330 66
PstI C_TGCA'G
Cuts at: 830 2743 4859 830
Size: 1913 2116 3478
Fragments arranged by size:
3478 2116 1913
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7507
PvuII CAG'CTG
Cuts at: 2106 4327 2106
Size: 2221 5286
SacI G_AGCT'C
Cuts at: 2837 3389 2837
Size: 552 6955
SalI G'TCGA_C
Cuts at: 1936 4849 1936
Size: 2913 4594
SbfI CC_TGCA'GG
Cuts at: 4859 4859
Size: 7507
ScaI AGT'ACT
Cuts at: 2168 4052 5755 7467 2168
Size: 1884 1703 1712 2208
Fragments arranged by size:
2208 1884 1712 1703
SfcI C'TryA_G
Cuts at: 826 1045 2739 4381 4855 5990 6671 6862 826
Size: 219 1694 1642 474 1135 681 191 1471
Fragments arranged by size:
1694 1642 1471 1135 681 474 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7507
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7507
SmaI CCC'GGG
Cuts at: 1872 4834 1872
Size: 2962 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2818 5611 6512 6774 820
Size: 197 926 875 2793 901 262 1553
Fragments arranged by size:
2793 1553 926 901 875 262 197
SnaBI TAC'GTA
Cuts at: 493 5298 493
Size: 4805 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7507
SphI G_CATG'C
Cuts at: 1132 4865 1132
Size: 3733 3774
SspI AAT'ATT
Cuts at: 5 52 3075 5431 5
Size: 47 3023 2356 2081
Fragments arranged by size:
3023 2356 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7507
StyI C'CwwG_G
Cuts at: 513 1065 2004 2270 4946 513
Size: 552 939 266 2676 3074
Fragments arranged by size:
3074 2676 939 552 266
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4279 5534 5551 5704 5889 6334 325
Size: 1255 2699 1255 17 153 185 445 1498
Fragments arranged by size:
2699 1498 1255 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2025 2166 2572
Size: 274 80 33 51 1443 48 141 406
Cuts at: 2572 3063 4050 5753 7465 96
Size: 491 987 1703 1712 138
Fragments arranged by size:
1712 1703 1443 987 491 406 274 141
138 80 51 48 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3245 3277 3718 3750 3790 3822 7467 7499 3245
Size: 32 441 32 40 32 3645 32 3253
Fragments arranged by size:
3645 3253 441 40 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7507
XbaI T'CTAG_A
Cuts at: 3043 4843 3043
Size: 1800 5707
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3478 4074 3478
Size: 596 6911
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7507
XmaI C'CCGG_G
Cuts at: 1870 4832 1870
Size: 2962 4545
XmnI GAAnn'nnTTC
Cuts at: 5636 5636
Size: 7507
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5315 276
Size: 53 83 186 936 3781 2468
Fragments arranged by size:
3781 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI