Restriction map for
pFN21AE1390
(Circular) (Six-base) MAPSORT of: pFN21AE1390.seq Check: 2009 from: 1 to: 5836
pFN21AE1390 5836 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3646 278
Size: 53 83 186 936 2110 2468
Fragments arranged by size:
2468 2110 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3179 1937
Size: 1242 4594
Acc65I G'GTAC_C
Cuts at: 3157 3157
Size: 5836
AclI AA'CG_TT
Cuts at: 1563 3963 4336 1563
Size: 2400 373 3063
Fragments arranged by size:
3063 2400 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2231 3899 5283 5551 1632
Size: 599 1668 1384 268 1917
Fragments arranged by size:
1917 1668 1384 599 268
AfeI AGC'GCT
Cuts at: 2771 2771
Size: 5836
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5639
AflIII A'CryG_T
Cuts at: 1203 4735 5712 1203
Size: 3532 977 1327
Fragments arranged by size:
3532 1327 977
AgeI A'CCGG_T
Cuts at: 4667 4667
Size: 5836
AhdI GACnn_n'nnGTC
Cuts at: 4565 4565
Size: 5836
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5836
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5836
AlwNI CAG_nnn'CTG
Cuts at: 3579 5151 3579
Size: 1572 4264
ApaLI G'TGCA_C
Cuts at: 3893 5049 3893
Size: 1156 4680
ApoI r'AATT_y
Cuts at: 1493 3145 3371 3523 5632 1493
Size: 1652 226 152 2109 1697
Fragments arranged by size:
2109 1697 1652 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4390 160
Size: 872 3358 1606
Fragments arranged by size:
3358 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
AvaI C'yCGr_G
Cuts at: 1870 1943 2303 3161 1870
Size: 73 360 858 4545
Fragments arranged by size:
4545 858 360 73
BamHI G'GATC_C
Cuts at: 3166 3533 3166
Size: 367 5469
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2127 2268 2915 3157 4612
Size: 325 217 627 340 141 647 242 1455
Cuts at: 4612 618
Size: 1842
Fragments arranged by size:
1842 1455 647 627 340 325 242 217
141
BanII G_rGCy'C
Cuts at: 1391 2292 3041 3155 1391
Size: 901 749 114 4072
Fragments arranged by size:
4072 901 749 114
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2092 928
Size: 971 193 4672
Fragments arranged by size:
4672 971 193
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3115 3149 4027 4061 1903
Size: 34 1178 34 878 34 3678
Fragments arranged by size:
3678 1178 878 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2226 3728 4938 2226
Size: 1502 1210 3124
Fragments arranged by size:
3124 1502 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5836
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2420 4447 136
Size: 107 122 71 631 605 748 2027 1525
Fragments arranged by size:
2027 1525 748 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2262 5831 2262
Size: 3569 2267
BlpI GC'TnA_GC
Cuts at: 3253 3253
Size: 5836
Bme1580I G_kGCm'C
Cuts at: 1790 3897 5053 1790
Size: 2107 1156 2573
Fragments arranged by size:
2573 2107 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5836
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2254 3567 4525
Size: 416 53 83 399 24 837 1313 958
Cuts at: 4525 442
Size: 1753
Fragments arranged by size:
1753 1313 958 837 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5836
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2738 2931 4496 1239
Size: 1499 193 1565 2579
Fragments arranged by size:
2579 1565 1499 193
Bpu10I CC'TnA_GC
Cuts at: 3009 3009
Size: 5836
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3961 4826 5124 3961
Size: 865 298 4673
Fragments arranged by size:
4673 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2015 2378 4499 882
Size: 617 516 363 2121 2219
Fragments arranged by size:
2219 2121 617 516 363
BsaAI yAC'GTr
Cuts at: 493 1204 2170 2410 3627 493
Size: 711 966 240 1217 2702
Fragments arranged by size:
2702 1217 966 711 240
BsaBI GATnn'nnATC
Cuts at: 5830 5830
Size: 5836
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3643 4025 4660 275
Size: 53 83 186 936 2110 382 635 1451
Fragments arranged by size:
2110 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4269 4667 4941 5088 5499 4269
Size: 398 274 147 411 4606
Fragments arranged by size:
4606 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2127 2157 1178
Size: 30 165 30 724 30 4857
Fragments arranged by size:
4857 724 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2321 2701 1186
Size: 847 288 380 4321
Fragments arranged by size:
4321 847 380 288
BseYI C'CCAG_C
Cuts at: 5039 5535 5039
Size: 496 5340
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5263
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3286 4047 5075 1540
Size: 275 186 1285 761 1028 2301
Fragments arranged by size:
2301 1285 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2292 2313 3897 3982 5053 2292
Size: 21 1584 85 1071 3075
Fragments arranged by size:
3075 1584 1071 85 21
BsmI GAATG_Cn'
Cuts at: 3347 3440 3347
Size: 93 5743
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2292 2313 3041 3155 3897 3982 5053
Size: 399 502 21 728 114 742 85 1071
Cuts at: 5053 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 728 502 399 114 85
21
BspEI T'CCGG_A
Cuts at: 5499 5499
Size: 5836
BspHI T'CATG_A
Cuts at: 2577 2856 3725 5455 2577
Size: 279 869 1730 2958
Fragments arranged by size:
2958 1730 869 279
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3191 844
Size: 2347 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3723 1394
Size: 429 1900 3507
Fragments arranged by size:
3507 1900 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2770 4331 4505 65
Size: 1183 1522 1561 174 1396
Fragments arranged by size:
1561 1522 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2066 4480 4667 1700
Size: 110 256 2414 187 2869
Fragments arranged by size:
2869 2414 256 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5836
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5836
BssSI C'ACGA_G
Cuts at: 3896 4908 3896
Size: 1012 4824
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5836
BstBI TT'CG_AA
Cuts at: 4640 4640
Size: 5836
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5836
BstXI CCAn_nnnn'nTGG
Cuts at: 2032 2410 2032
Size: 378 5458
BstYI r'GATC_y
Cuts at: 1972 2262 2545 2583 3166 3533 3918 3935 5387
Size: 290 283 38 583 367 385 17 1452
Cuts at: 5387 5473 5485 5831 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 583 385 367 346 290 283
86 38 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2123 513
Size: 552 1058 4226
Fragments arranged by size:
4226 1058 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2094 487
Size: 683 56 868 4229
Fragments arranged by size:
4229 868 683 56
BtsI GCAGTG_nn'
Cuts at: 2430 2616 2961 3352 4146 4166 2430
Size: 186 345 391 794 20 4100
Fragments arranged by size:
4100 794 391 345 186 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5836
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2443 2478 529
Size: 35 1879 35 3887
Fragments arranged by size:
3887 1879 35 35
DraI TTT'AAA
Cuts at: 3140 3140
Size: 5836
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4843 809
Size: 507 3527 1802
Fragments arranged by size:
3527 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2412 2446 2691
Size: 54 1425 50 275 18 582 34 245
Cuts at: 2691 2743 3283 4172 5801 8
Size: 52 540 889 1629 43
Fragments arranged by size:
1629 1425 889 582 540 275 245 54
52 50 43 34 18
EagI C'GGCC_G
Cuts at: 1537 1812 3283 1537
Size: 275 1471 4090
Fragments arranged by size:
4090 1471 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2710 2717 2798 3766 1353
Size: 1357 7 81 968 3423
Fragments arranged by size:
3423 1357 968 81 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4423 4797 4943 387
Size: 1064 138 2834 374 146 1280
Fragments arranged by size:
2834 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2290 2290
Size: 5836
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2231 2738 2931 3899 4496 5283 5551
Size: 393 599 507 193 968 597 787 268
Cuts at: 5551 1239
Size: 1524
Fragments arranged by size:
1524 968 787 599 597 507 393 268
193
EcoO109I rG'GnC_Cy
Cuts at: 2332 2560 2884 2332
Size: 228 324 5284
Fragments arranged by size:
5284 324 228
EcoRI G'AATT_C
Cuts at: 3145 3523 3145
Size: 378 5458
EcoRV GAT'ATC
Cuts at: 2875 2875
Size: 5836
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2086 2086
Size: 5836
FspI TGC'GCA
Cuts at: 4342 4342
Size: 5836
HaeII r_GCGC'y
Cuts at: 1678 2773 4983 1678
Size: 1095 2210 2531
Fragments arranged by size:
2531 2210 1095
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2033 2065 2986 3018 4477
Size: 32 135 32 461 32 921 32 1459
Cuts at: 4477 4509 4551 4583 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1459 921 461 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3180 3426 669
Size: 988 281 1242 246 3079
Fragments arranged by size:
3079 1242 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5836
HpaI GTT'AAC
Cuts at: 3426 3426
Size: 5836
KpnI G_GTAC'C
Cuts at: 3161 3161
Size: 5836
MfeI C'AATT_G
Cuts at: 3435 3435
Size: 5836
MluI A'CGCG_T
Cuts at: 5712 5712
Size: 5836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4950 5134 1305
Size: 3645 184 2007
Fragments arranged by size:
3645 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2414 2693 2745 10
Size: 54 1425 343 582 279 52 3101
Fragments arranged by size:
3101 1425 582 343 279 54 52
MslI CAynn'nnrTG
Cuts at: 518 1166 2408 2813 2921 3794 4153 4312 518
Size: 648 1242 405 108 873 359 159 2042
Fragments arranged by size:
2042 1242 873 648 405 359 159 108
MspA1I CmG'CkG
Cuts at: 1606 2125 2338 2699 2778 3252 3929 5077 5322
Size: 519 213 361 79 474 677 1148 245
Cuts at: 5322 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 519 474 361 245 213
79
NaeI GCC'GGC
Cuts at: 2068 2068
Size: 5836
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5284
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5836
NgoMIV G'CCGG_C
Cuts at: 2066 2066
Size: 5836
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5836
NotI GC'GGCC_GC
Cuts at: 3283 3283
Size: 5836
NspI r_CATG'y
Cuts at: 1132 1229 3194 4739 1132
Size: 97 1965 1545 2229
Fragments arranged by size:
2229 1965 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5836
PciI A'CATG_T
Cuts at: 4735 4735
Size: 5836
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5836
PmeI GTTT'AAAC
Cuts at: 3140 3140
Size: 5836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3905 3937 5418 5450 3905
Size: 32 1481 32 4291
Fragments arranged by size:
4291 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2560 2884 2560
Size: 324 5512
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5836
PsiI TTA'TAA
Cuts at: 3406 3406
Size: 5836
PstI C_TGCA'G
Cuts at: 830 3188 830
Size: 2358 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5836
PvuII CAG'CTG
Cuts at: 2699 2699
Size: 5836
SacI G_AGCT'C
Cuts at: 2292 2292
Size: 5836
SacII CC_GC'GG
Cuts at: 2126 2126
Size: 5836
SalI G'TCGA_C
Cuts at: 1936 3178 1936
Size: 1242 4594
SapI GCTCTTCn'nnn_
Cuts at: 2717 2717
Size: 5836
SbfI CC_TGCA'GG
Cuts at: 3188 3188
Size: 5836
ScaI AGT'ACT
Cuts at: 4084 5796 4084
Size: 1712 4124
SfcI C'TryA_G
Cuts at: 826 1045 3184 4319 5000 5191 826
Size: 219 2139 1135 681 191 1471
Fragments arranged by size:
2139 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5836
SmaI CCC'GGG
Cuts at: 1872 3163 1872
Size: 1291 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3940 4841 5103 820
Size: 197 926 1997 901 262 1553
Fragments arranged by size:
1997 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2170 3627 493
Size: 1677 1457 2702
Fragments arranged by size:
2702 1677 1457
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5836
SphI G_CATG'C
Cuts at: 1132 3194 1132
Size: 2062 3774
SspI AAT'ATT
Cuts at: 5 52 3760 5
Size: 47 3708 2081
Fragments arranged by size:
3708 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5836
StyI C'CwwG_G
Cuts at: 513 1065 2346 3275 513
Size: 552 1281 929 3074
Fragments arranged by size:
3074 1281 929 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3863 3880 4033 4218 4663 325
Size: 1255 2283 17 153 185 445 1498
Fragments arranged by size:
2283 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4082 5794 96
Size: 274 80 33 51 1443 2105 1712 138
Fragments arranged by size:
2105 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2127 2159 5796 5828 2127
Size: 32 3637 32 2135
Fragments arranged by size:
3637 2135 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5836
XbaI T'CTAG_A
Cuts at: 3172 3172
Size: 5836
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2539 2539
Size: 5836
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5836
XmaI C'CCGG_G
Cuts at: 1870 3161 1870
Size: 1291 4545
XmnI GAAnn'nnTTC
Cuts at: 3965 3965
Size: 5836
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3644 276
Size: 53 83 186 936 2110 2468
Fragments arranged by size:
2468 2110 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SanDI SexAI SfiI
SfoI SrfI SwaI