Restriction map for
pFN21AE1388
(Circular) (Six-base) MAPSORT of: pFN21AE1388.seq Check: 3504 from: 1 to: 6256
pFN21AE1388 6256 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4066 278
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2432 2474 3599 1937
Size: 495 42 1125 4594
Fragments arranged by size:
4594 1125 495 42
Acc65I G'GTAC_C
Cuts at: 3577 3577
Size: 6256
AclI AA'CG_TT
Cuts at: 1563 4383 4756 1563
Size: 2820 373 3063
Fragments arranged by size:
3063 2820 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2250 2688 2841 4319 5703 5971 1632
Size: 618 438 153 1478 1384 268 1917
Fragments arranged by size:
1917 1478 1384 618 438 268 153
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6059
AflIII A'CryG_T
Cuts at: 1203 5155 6132 1203
Size: 3952 977 1327
Fragments arranged by size:
3952 1327 977
AgeI A'CCGG_T
Cuts at: 3054 5087 3054
Size: 2033 4223
AhdI GACnn_n'nnGTC
Cuts at: 2789 4985 2789
Size: 2196 4060
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6256
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6256
AlwNI CAG_nnn'CTG
Cuts at: 2467 2648 2984 3999 5571 2467
Size: 181 336 1015 1572 3152
Fragments arranged by size:
3152 1572 1015 336 181
ApaLI G'TGCA_C
Cuts at: 4313 5469 4313
Size: 1156 5100
ApoI r'AATT_y
Cuts at: 1493 3119 3335 3565 3791 3943 6052 1493
Size: 1626 216 230 226 152 2109 1697
Fragments arranged by size:
2109 1697 1626 230 226 216 152
AseI AT'TA_AT
Cuts at: 160 1032 4810 160
Size: 872 3778 1606
Fragments arranged by size:
3778 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
AvaI C'yCGr_G
Cuts at: 1870 1943 3581 1870
Size: 73 1638 4545
Fragments arranged by size:
4545 1638 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2905 2938 2905
Size: 33 6223
BamHI G'GATC_C
Cuts at: 2025 3586 3953 2025
Size: 1561 367 4328
Fragments arranged by size:
4328 1561 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3577 5032 618
Size: 325 217 627 1790 1455 1842
Fragments arranged by size:
1842 1790 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3318 3575 1391
Size: 1927 257 4072
Fragments arranged by size:
4072 1927 257
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2238 2694 2895 928
Size: 971 339 456 201 4289
Fragments arranged by size:
4289 971 456 339 201
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4447 4481 1903
Size: 34 2510 34 3678
Fragments arranged by size:
3678 2510 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4148 5358 4148
Size: 1210 5046
BclI T'GATC_A
Cuts at: 1548 2100 3340 1548
Size: 552 1240 4464
Fragments arranged by size:
4464 1240 552
BfrBI ATG'CAT
Cuts at: 2625 2625
Size: 6256
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4867 136
Size: 107 122 71 631 605 3195 1525
Fragments arranged by size:
3195 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6251 6251
Size: 6256
BlpI GC'TnA_GC
Cuts at: 3673 3673
Size: 6256
Bme1580I G_kGCm'C
Cuts at: 1790 4317 5473 1790
Size: 2527 1156 2573
Fragments arranged by size:
2573 2527 1156
BmgBI CAC'GTC
Cuts at: 1318 2221 1318
Size: 903 5353
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2455 2518 2825
Size: 416 53 83 399 24 1038 63 307
Cuts at: 2825 3987 4945 442
Size: 1162 958 1753
Fragments arranged by size:
1753 1162 1038 958 416 399 307 83
63 53 24
BmtI G_CTAG'C
Cuts at: 1056 2936 1056
Size: 1880 4376
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2033 2781 4916 1239
Size: 794 748 2135 2579
Fragments arranged by size:
2579 2135 794 748
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4381 5246 5544 4381
Size: 865 298 5093
Fragments arranged by size:
5093 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4919 882
Size: 617 3420 2219
Fragments arranged by size:
3420 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4047 493
Size: 711 2843 2702
Fragments arranged by size:
2843 2702 711
BsaBI GATnn'nnATC
Cuts at: 6250 6250
Size: 6256
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4063 4445 5080 275
Size: 53 83 186 936 2530 382 635 1451
Fragments arranged by size:
2530 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3054 4689 5087 5361 5508 5919 3054
Size: 1635 398 274 147 411 3391
Fragments arranged by size:
3391 1635 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2754 2784 3029 3059 1178
Size: 30 165 30 1351 30 245 30 4375
Fragments arranged by size:
4375 1351 245 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 2472 1186
Size: 844 442 4970
Fragments arranged by size:
4970 844 442
BseYI C'CCAG_C
Cuts at: 5459 5955 5459
Size: 496 5760
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5683
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3706 4467 5495 1540
Size: 275 186 1705 761 1028 2301
Fragments arranged by size:
2301 1705 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3318 4317 4402 5473 3318
Size: 999 85 1071 4101
Fragments arranged by size:
4101 1071 999 85
BsmI GAATG_Cn'
Cuts at: 3767 3860 3767
Size: 93 6163
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3318 3575 4317 4402 5473 1391
Size: 399 1528 257 742 85 1071 2174
Fragments arranged by size:
2174 1528 1071 742 399 257 85
BspEI T'CCGG_A
Cuts at: 5919 5919
Size: 6256
BspHI T'CATG_A
Cuts at: 4145 5875 4145
Size: 1730 4526
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3611 844
Size: 2767 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4143 1394
Size: 429 2320 3507
Fragments arranged by size:
3507 2320 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3318 4751 4925 65
Size: 1183 2070 1433 174 1396
Fragments arranged by size:
2070 1433 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3054 4900 5087 1700
Size: 110 1244 1846 187 2869
Fragments arranged by size:
2869 1846 1244 187 110
BsrGI T'GTAC_A
Cuts at: 96 3409 3512 96
Size: 3313 103 2840
Fragments arranged by size:
3313 2840 103
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6256
BssSI C'ACGA_G
Cuts at: 2114 4316 5328 2114
Size: 2202 1012 3042
Fragments arranged by size:
3042 2202 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6256
BstBI TT'CG_AA
Cuts at: 2886 5060 2886
Size: 2174 4082
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6256
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2083 3473 2011
Size: 72 1390 4794
Fragments arranged by size:
4794 1390 72
BstYI r'GATC_y
Cuts at: 1972 2025 2599 3586 3953 4338 4355 5807 5893
Size: 53 574 987 367 385 17 1452 86
Cuts at: 5893 5905 6251 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 987 574 385 367 346 86
53 17 12
BstZ17I GTA'TAC
Cuts at: 2475 2475
Size: 6256
Bsu36I CC'TnA_GG
Cuts at: 2062 2484 2062
Size: 422 5834
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4765
Fragments arranged by size:
4765 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2041 487
Size: 683 56 815 4702
Fragments arranged by size:
4702 815 683 56
BtsI GCAGTG_nn'
Cuts at: 3772 4566 4586 3772
Size: 794 20 5442
Fragments arranged by size:
5442 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6256
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6221
DraI TTT'AAA
Cuts at: 3145 3430 3560 3145
Size: 285 130 5841
Fragments arranged by size:
5841 285 130
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5263 809
Size: 507 3947 1802
Fragments arranged by size:
3947 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2923 3703 4592
Size: 54 1425 50 275 18 1093 780 889
Cuts at: 4592 6221 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1093 889 780 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3703 1537
Size: 275 1891 4090
Fragments arranged by size:
4090 1891 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3306 4186 1353
Size: 1953 880 3423
Fragments arranged by size:
3423 1953 880
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4843 5217 5363 387
Size: 1064 138 3254 374 146 1280
Fragments arranged by size:
3254 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3316 3316
Size: 6256
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2250 2688 2781 2841 4319 4916
Size: 393 401 217 438 93 60 1478 597
Cuts at: 4916 5703 5971 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1478 787 597 438 401 393 268
217 93 60
EcoRI G'AATT_C
Cuts at: 3565 3943 3565
Size: 378 5878
FspI TGC'GCA
Cuts at: 4762 4762
Size: 6256
HaeII r_GCGC'y
Cuts at: 1678 5403 1678
Size: 3725 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2775 2795 2807 2827 4897
Size: 32 135 32 1203 20 12 20 2070
Cuts at: 4897 4929 4971 5003 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2070 1203 135 42 32 32 32
32 20 20 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3600 3846 669
Size: 988 281 1662 246 3079
Fragments arranged by size:
3079 1662 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2405 748
Size: 1657 4599
HpaI GTT'AAC
Cuts at: 3846 3846
Size: 6256
KpnI G_GTAC'C
Cuts at: 3581 3581
Size: 6256
MfeI C'AATT_G
Cuts at: 3177 3855 3177
Size: 678 5578
MluI A'CGCG_T
Cuts at: 6132 6132
Size: 6256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5370 5554 1305
Size: 4065 184 2007
Fragments arranged by size:
4065 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2925 10
Size: 54 1425 343 1093 3341
Fragments arranged by size:
3341 1425 1093 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2577 4214 4573 4732 518
Size: 648 1411 1637 359 159 2042
Fragments arranged by size:
2042 1637 1411 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2083 3672 4349 5497 5742 1606
Size: 477 1589 677 1148 245 2120
Fragments arranged by size:
2120 1589 1148 677 477 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4765
Fragments arranged by size:
4765 939 552
NdeI CA'TA_TG
Cuts at: 387 3031 387
Size: 2644 3612
NheI G'CTAG_C
Cuts at: 1052 2932 1052
Size: 1880 4376
NotI GC'GGCC_GC
Cuts at: 3703 3703
Size: 6256
NsiI A_TGCA'T
Cuts at: 2627 2627
Size: 6256
NspI r_CATG'y
Cuts at: 1132 1229 2099 3614 5159 1132
Size: 97 870 1515 1545 2229
Fragments arranged by size:
2229 1545 1515 870 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6256
PciI A'CATG_T
Cuts at: 5155 5155
Size: 6256
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2467 1108
Size: 1359 4897
PmeI GTTT'AAAC
Cuts at: 3560 3560
Size: 6256
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4325 4357 5838 5870 4325
Size: 32 1481 32 4711
Fragments arranged by size:
4711 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6256
PsiI TTA'TAA
Cuts at: 2567 3826 2567
Size: 1259 4997
PstI C_TGCA'G
Cuts at: 830 2127 3608 830
Size: 1297 1481 3478
Fragments arranged by size:
3478 1481 1297
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6256
PvuII CAG'CTG
Cuts at: 2083 2083
Size: 6256
SacI G_AGCT'C
Cuts at: 3318 3318
Size: 6256
SalI G'TCGA_C
Cuts at: 1936 3598 1936
Size: 1662 4594
SapI GCTCTTCn'nnn_
Cuts at: 3306 3306
Size: 6256
SbfI CC_TGCA'GG
Cuts at: 3608 3608
Size: 6256
ScaI AGT'ACT
Cuts at: 4504 6216 4504
Size: 1712 4544
SexAI A'CCwGG_T
Cuts at: 2279 2279
Size: 6256
SfcI C'TryA_G
Cuts at: 826 1045 2123 3540 3604 4739 5420 5611 826
Size: 219 1078 1417 64 1135 681 191 1471
Fragments arranged by size:
1471 1417 1135 1078 681 219 191 64
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6256
SmaI CCC'GGG
Cuts at: 1872 3583 1872
Size: 1711 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4360 5261 5523 820
Size: 197 926 2417 901 262 1553
Fragments arranged by size:
2417 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4047 493
Size: 3554 2702
SpeI A'CTAG_T
Cuts at: 152 2159 152
Size: 2007 4249
SphI G_CATG'C
Cuts at: 1132 3614 1132
Size: 2482 3774
SspI AAT'ATT
Cuts at: 5 52 4180 5
Size: 47 4128 2081
Fragments arranged by size:
4128 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6256
StyI C'CwwG_G
Cuts at: 513 1065 2004 3695 513
Size: 552 939 1691 3074
Fragments arranged by size:
3074 1691 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2560 2683 4283 4300 4453 4638 5083
Size: 1255 980 123 1600 17 153 185 445
Cuts at: 5083 325
Size: 1498
Fragments arranged by size:
1600 1498 1255 980 445 185 153 123
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3409 3512 4502
Size: 274 80 33 51 1443 1432 103 990
Cuts at: 4502 6214 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1432 990 274 138 103 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2682 2714 2754 2786 2817 2849 3029 3061 6216
Size: 32 40 32 31 32 180 32 3155
Cuts at: 6216 6248 2682
Size: 32 2690
Fragments arranged by size:
3155 2690 180 40 32 32 32 32
32 31
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6256
XbaI T'CTAG_A
Cuts at: 3592 3592
Size: 6256
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6256
XmaI C'CCGG_G
Cuts at: 1870 3581 1870
Size: 1711 4545
XmnI GAAnn'nnTTC
Cuts at: 2340 4385 2340
Size: 2045 4211
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4064 276
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BplI Bpu10I BsiWI BsmBI DraIII EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SfiI SfoI SrfI SwaI XcmI