Restriction map for
pFN21AE1387
(Circular) (Six-base) MAPSORT of: pFN21AE1387.seq Check: 2524 from: 1 to: 7651
pFN21AE1387 7651 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4351 4351
Size: 7651
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5461 278
Size: 53 83 186 936 3925 2468
Fragments arranged by size:
3925 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3721 3826 4994 1937
Size: 1784 105 1168 4594
Fragments arranged by size:
4594 1784 1168 105
Acc65I G'GTAC_C
Cuts at: 4972 4972
Size: 7651
AclI AA'CG_TT
Cuts at: 1563 5778 6151 1563
Size: 4215 373 3063
Fragments arranged by size:
4215 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2183 2437 4239 4554 5714 7098 7366 1632
Size: 551 254 1802 315 1160 1384 268 1917
Fragments arranged by size:
1917 1802 1384 1160 551 315 268 254
AflII C'TTAA_G
Cuts at: 820 1017 3655 4282 820
Size: 197 2638 627 4189
Fragments arranged by size:
4189 2638 627 197
AflIII A'CryG_T
Cuts at: 1203 6550 7527 1203
Size: 5347 977 1327
Fragments arranged by size:
5347 1327 977
AgeI A'CCGG_T
Cuts at: 6482 6482
Size: 7651
AhdI GACnn_n'nnGTC
Cuts at: 6380 6380
Size: 7651
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7651
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3356 1184
Size: 2172 5479
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4231 4263 4231
Size: 32 7619
AlwNI CAG_nnn'CTG
Cuts at: 2537 4322 5394 6966 2537
Size: 1785 1072 1572 3222
Fragments arranged by size:
3222 1785 1572 1072
ApaLI G'TGCA_C
Cuts at: 3181 3412 5708 6864 3181
Size: 231 2296 1156 3968
Fragments arranged by size:
3968 2296 1156 231
ApoI r'AATT_y
Cuts at: 1493 2067 2543 2699 2755 4078 4162 4752 4910
Size: 574 476 156 56 1323 84 590 158
Cuts at: 4910 4960 5186 5338 7447 1493
Size: 50 226 152 2109 1697
Fragments arranged by size:
2109 1697 1323 590 574 476 226 158
156 152 84 56 50
AseI AT'TA_AT
Cuts at: 160 1032 6205 160
Size: 872 5173 1606
Fragments arranged by size:
5173 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7651
AvaI C'yCGr_G
Cuts at: 1870 1943 3789 4359 4976 1870
Size: 73 1846 570 617 4545
Fragments arranged by size:
4545 1846 617 570 73
AvrII C'CTAG_G
Cuts at: 3832 3832
Size: 7651
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2503 2536 2562 2595 2503
Size: 33 26 33 7559
Fragments arranged by size:
7559 33 33 26
BamHI G'GATC_C
Cuts at: 3274 3449 4981 5348 3274
Size: 175 1532 367 5577
Fragments arranged by size:
5577 1532 367 175
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3783 4705 4972 6427 618
Size: 325 217 627 1996 922 267 1455 1842
Fragments arranged by size:
1996 1842 1455 922 627 325 267 217
BanII G_rGCy'C
Cuts at: 1391 2738 3107 3264 3845 3905 4970 1391
Size: 1347 369 157 581 60 1065 4072
Fragments arranged by size:
4072 1347 1065 581 369 157 60
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2153 3591 928
Size: 971 254 1438 4988
Fragments arranged by size:
4988 1438 971 254
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5842 5876 1903
Size: 34 3905 34 3678
Fragments arranged by size:
3905 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4942 5543 6753 4942
Size: 601 1210 5840
Fragments arranged by size:
5840 1210 601
BclI T'GATC_A
Cuts at: 1548 4672 1548
Size: 3124 4527
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3169 6262 136
Size: 107 122 71 631 605 1497 3093 1525
Fragments arranged by size:
3093 1525 1497 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3563 7646 3563
Size: 4083 3568
BlpI GC'TnA_GC
Cuts at: 5068 5068
Size: 7651
Bme1580I G_kGCm'C
Cuts at: 1790 3185 3416 3788 5712 6868 1790
Size: 1395 231 372 1924 1156 2573
Fragments arranged by size:
2573 1924 1395 1156 372 231
BmgBI CAC'GTC
Cuts at: 1318 4940 1318
Size: 3622 4029
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2916 3905 4100
Size: 416 53 83 399 24 1499 989 195
Cuts at: 4100 5382 6340 442
Size: 1282 958 1753
Fragments arranged by size:
1753 1499 1282 989 958 416 399 195
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7651
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2569 3286 3479 4014 4510 4911 6311 1239
Size: 1330 717 193 535 496 401 1400 2579
Fragments arranged by size:
2579 1400 1330 717 535 496 401 193
Bpu10I CC'TnA_GC
Cuts at: 2112 3960 2112
Size: 1848 5803
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5776 6641 6939 5776
Size: 865 298 6488
Fragments arranged by size:
6488 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4095 6314 882
Size: 617 2596 2219 2219
Fragments arranged by size:
2596 2219 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5442 493
Size: 711 4238 2702
Fragments arranged by size:
4238 2702 711
BsaBI GATnn'nnATC
Cuts at: 2247 3363 4410 7645 2247
Size: 1116 1047 3235 2253
Fragments arranged by size:
3235 2253 1116 1047
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3934 4397 5458 5840
Size: 53 83 186 936 2401 463 1061 382
Cuts at: 5840 6475 275
Size: 635 1451
Fragments arranged by size:
2401 1451 1061 936 635 463 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2859 6084 6482 6756 6903 7314 2859
Size: 3225 398 274 147 411 3196
Fragments arranged by size:
3225 3196 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2384 2414 3948 3978 1178
Size: 30 165 30 981 30 1534 30 4851
Fragments arranged by size:
4851 1534 981 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2058 3022 3386 3519 3639 3657 4011 4014
Size: 872 964 364 133 120 18 354 3
Cuts at: 4014 4017 4034 4914 1186
Size: 3 17 880 3923
Fragments arranged by size:
3923 964 880 872 364 354 133 120
18 17 3 3
BseYI C'CCAG_C
Cuts at: 3637 6854 7350 3637
Size: 3217 496 3938
Fragments arranged by size:
3938 3217 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3153 4728 1163
Size: 573 1417 1575 4086
Fragments arranged by size:
4086 1575 1417 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4733 5101 5862 6890 1540
Size: 275 186 2732 368 761 1028 2301
Fragments arranged by size:
2732 2301 1028 761 368 275 186
BsiHKAI G_wGCw'C
Cuts at: 3185 3416 5712 5797 6868 3185
Size: 231 2296 85 1071 3968
Fragments arranged by size:
3968 2296 1071 231 85
BsmI GAATG_Cn'
Cuts at: 5162 5255 5162
Size: 93 7558
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2738 3107 3185 3264 3416 3788 3845
Size: 399 948 369 78 79 152 372 57
Cuts at: 3845 3905 4970 5712 5797 6868 1391
Size: 60 1065 742 85 1071 2174
Fragments arranged by size:
2174 1071 1065 948 742 399 372 369
152 85 79 78 60 57
BspEI T'CCGG_A
Cuts at: 7314 7314
Size: 7651
BspHI T'CATG_A
Cuts at: 2608 3052 5540 7270 2608
Size: 444 2488 1730 2989
Fragments arranged by size:
2989 2488 1730 444
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4351 5006 844
Size: 3507 655 3489
Fragments arranged by size:
3507 3489 655
BsrBI CCG'CTC
Cuts at: 1394 1823 5538 1394
Size: 429 3715 3507
Fragments arranged by size:
3715 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2649 2683 6146 6320 65
Size: 1183 1401 34 3463 174 1396
Fragments arranged by size:
3463 1401 1396 1183 174 34
BsrFI r'CCGG_y
Cuts at: 1700 1810 4533 6295 6482 1700
Size: 110 2723 1762 187 2869
Fragments arranged by size:
2869 2723 1762 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7651
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7651
BssSI C'ACGA_G
Cuts at: 5711 6723 5711
Size: 1012 6639
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2537 3189 4811 794
Size: 1743 652 1622 3634
Fragments arranged by size:
3634 1743 1622 652
BstBI TT'CG_AA
Cuts at: 6455 6455
Size: 7651
BstEII G'GTnAC_C
Cuts at: 1184 3977 4626 1184
Size: 2793 649 4209
Fragments arranged by size:
4209 2793 649
BstXI CCAn_nnnn'nTGG
Cuts at: 3271 3271
Size: 7651
BstYI r'GATC_y
Cuts at: 1972 3274 3449 3498 3538 3563 3661 4552 4573
Size: 1302 175 49 40 25 98 891 21
Cuts at: 4573 4981 5348 5733 5750 7202 7288 7300 7646
Size: 408 367 385 17 1452 86 12 346
Cuts at: 7646 1972
Size: 1977
Fragments arranged by size:
1977 1452 1302 891 408 385 367 346
175 98 86 49 40 25 21 17
12
Bsu36I CC'TnA_GG
Cuts at: 4543 4779 4543
Size: 236 7415
BtgI C'CryG_G
Cuts at: 513 1065 2004 2139 2582 4771 513
Size: 552 939 135 443 2189 3393
Fragments arranged by size:
3393 2189 939 552 443 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6912
Fragments arranged by size:
6912 683 56
BtsI GCAGTG_nn'
Cuts at: 5167 5961 5981 5167
Size: 794 20 6837
Fragments arranged by size:
6837 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7651
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7616
DraI TTT'AAA
Cuts at: 2326 2380 4296 4955 2326
Size: 54 1916 659 5022
Fragments arranged by size:
5022 1916 659 54
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3149 6658 809
Size: 507 1833 3509 1802
Fragments arranged by size:
3509 1833 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4535 4597 5098
Size: 54 1425 50 275 18 2705 62 501
Cuts at: 5098 5987 7616 8
Size: 889 1629 43
Fragments arranged by size:
2705 1629 1425 889 501 275 62 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5098 1537
Size: 275 3286 4090
Fragments arranged by size:
4090 3286 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4839 4847 5581 1353
Size: 3486 8 734 3423
Fragments arranged by size:
3486 3423 734 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6238 6612 6758 387
Size: 1064 138 4649 374 146 1280
Fragments arranged by size:
4649 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 2437 2569 3286 3479 4014 4239
Size: 393 551 254 132 717 193 535 225
Cuts at: 4239 4510 4554 4911 5714 6311 7098 7366 1239
Size: 271 44 357 803 597 787 268 1524
Fragments arranged by size:
1524 803 787 717 597 551 535 393
357 271 268 254 225 193 132 44
EcoNI CCTnn'n_nnAGG
Cuts at: 2609 3836 3951 4428 2609
Size: 1227 115 477 5832
Fragments arranged by size:
5832 1227 477 115
EcoRI G'AATT_C
Cuts at: 2543 4162 4960 5338 2543
Size: 1619 798 378 4856
Fragments arranged by size:
4856 1619 798 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3765 4876 3765
Size: 1111 6540
FspI TGC'GCA
Cuts at: 6157 6157
Size: 7651
HaeII r_GCGC'y
Cuts at: 1678 6798 1678
Size: 5120 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2415 2447 2920 2952 3001
Size: 32 135 32 843 32 473 32 49
Cuts at: 3001 3033 3284 3316 3373 3405 3490 3522 3813
Size: 32 251 32 57 32 85 32 291
Cuts at: 3813 3845 3948 3980 4250 4282 4304 4336 6292
Size: 32 103 32 270 32 22 32 1956
Cuts at: 6292 6324 6366 6398 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1956 843 473 291 270 251 135
103 85 57 49 42 32 32 32
32 32 32 32 32 32 32 32
32 32 32 22
HincII GTy'rAC
Cuts at: 669 1657 1938 3827 4995 5241 669
Size: 988 281 1889 1168 246 3079
Fragments arranged by size:
3079 1889 1168 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7651
HpaI GTT'AAC
Cuts at: 5241 5241
Size: 7651
KpnI G_GTAC'C
Cuts at: 4976 4976
Size: 7651
MfeI C'AATT_G
Cuts at: 2661 5250 2661
Size: 2589 5062
MluI A'CGCG_T
Cuts at: 7527 7527
Size: 7651
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2083 3192 4379 6765 6949 1305
Size: 778 1109 1187 2386 184 2007
Fragments arranged by size:
2386 2007 1187 1109 778 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4599 10
Size: 54 1425 343 2767 3062
Fragments arranged by size:
3062 2767 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3269 3338 5609 5968 6127 518
Size: 648 2103 69 2271 359 159 2042
Fragments arranged by size:
2271 2103 2042 648 359 159 69
MspA1I CmG'CkG
Cuts at: 1606 5067 5744 6892 7137 1606
Size: 3461 677 1148 245 2120
Fragments arranged by size:
3461 2120 1148 677 245
NaeI GCC'GGC
Cuts at: 4535 4535
Size: 7651
NcoI C'CATG_G
Cuts at: 513 1065 2004 2582 4771 513
Size: 552 939 578 2189 3393
Fragments arranged by size:
3393 2189 939 578 552
NdeI CA'TA_TG
Cuts at: 387 2800 387
Size: 2413 5238
NgoMIV G'CCGG_C
Cuts at: 4533 4533
Size: 7651
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7651
NotI GC'GGCC_GC
Cuts at: 5098 5098
Size: 7651
NspI r_CATG'y
Cuts at: 1132 1229 2464 3337 5009 6554 1132
Size: 97 1235 873 1672 1545 2229
Fragments arranged by size:
2229 1672 1545 1235 873 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7651
PciI A'CATG_T
Cuts at: 6550 6550
Size: 7651
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3736 3854 4376 1108
Size: 2628 118 522 4383
Fragments arranged by size:
4383 2628 522 118
PmeI GTTT'AAAC
Cuts at: 4955 4955
Size: 7651
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5720 5752 7233 7265 5720
Size: 32 1481 32 6106
Fragments arranged by size:
6106 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7651
PsiI TTA'TAA
Cuts at: 3015 5221 3015
Size: 2206 5445
PstI C_TGCA'G
Cuts at: 830 5003 830
Size: 4173 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7651
SalI G'TCGA_C
Cuts at: 1936 3825 4993 1936
Size: 1889 1168 4594
Fragments arranged by size:
4594 1889 1168
SbfI CC_TGCA'GG
Cuts at: 5003 5003
Size: 7651
ScaI AGT'ACT
Cuts at: 2321 5899 7611 2321
Size: 3578 1712 2361
Fragments arranged by size:
3578 2361 1712
SexAI A'CCwGG_T
Cuts at: 4630 4630
Size: 7651
SfcI C'TryA_G
Cuts at: 826 1045 2822 4999 6134 6815 7006 826
Size: 219 1777 2177 1135 681 191 1471
Fragments arranged by size:
2177 1777 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7651
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7651
SmaI CCC'GGG
Cuts at: 1872 4978 1872
Size: 3106 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3655 4282 5755 6656 6918 820
Size: 197 926 1712 627 1473 901 262 1553
Fragments arranged by size:
1712 1553 1473 926 901 627 262 197
SnaBI TAC'GTA
Cuts at: 493 5442 493
Size: 4949 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7651
SphI G_CATG'C
Cuts at: 1132 3337 5009 1132
Size: 2205 1672 3774
Fragments arranged by size:
3774 2205 1672
SspI AAT'ATT
Cuts at: 5 52 5575 5
Size: 47 5523 2081
Fragments arranged by size:
5523 2081 47
StuI AGG'CCT
Cuts at: 1517 4351 1517
Size: 2834 4817
StyI C'CwwG_G
Cuts at: 513 1065 2004 2582 2729 3832 3982 4771 5090
Size: 552 939 578 147 1103 150 789 319
Cuts at: 5090 513
Size: 3074
Fragments arranged by size:
3074 1103 939 789 578 552 319 150
147
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4194 5678 5695 5848 6033 6478 325
Size: 1255 2614 1484 17 153 185 445 1498
Fragments arranged by size:
2614 1498 1484 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2319 4061 5897
Size: 274 80 33 51 1443 342 1742 1836
Cuts at: 5897 7609 96
Size: 1712 138
Fragments arranged by size:
1836 1742 1712 1443 342 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3483 3515 4139 4171 7611 7643 3483
Size: 32 624 32 3440 32 3491
Fragments arranged by size:
3491 3440 624 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7651
XbaI T'CTAG_A
Cuts at: 4987 4987
Size: 7651
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2627 2627
Size: 7651
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7651
XmaI C'CCGG_G
Cuts at: 1870 4976 1870
Size: 3106 4545
XmnI GAAnn'nnTTC
Cuts at: 2591 5780 2591
Size: 3189 4462
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5459 276
Size: 53 83 186 936 3925 2468
Fragments arranged by size:
3925 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BbeI BbvCI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoO109I EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI