Restriction map for
pFN21AE1384
(Circular) (Six-base) MAPSORT of: pFN21AE1384.seq Check: 6906 from: 1 to: 6499
pFN21AE1384 6499 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4309 278
Size: 53 83 186 936 2773 2468
Fragments arranged by size:
2773 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3842 1937
Size: 1905 4594
Acc65I G'GTAC_C
Cuts at: 3820 3820
Size: 6499
AclI AA'CG_TT
Cuts at: 1563 4626 4999 1563
Size: 3063 373 3063
Fragments arranged by size:
3063 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2146 2563 2645 3050 3629 4562 5946 6214
Size: 514 417 82 405 579 933 1384 268
Cuts at: 6214 1632
Size: 1917
Fragments arranged by size:
1917 1384 933 579 514 417 405 268
82
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6302
AflIII A'CryG_T
Cuts at: 1203 2852 5398 6375 1203
Size: 1649 2546 977 1327
Fragments arranged by size:
2546 1649 1327 977
AgeI A'CCGG_T
Cuts at: 5330 5330
Size: 6499
AhdI GACnn_n'nnGTC
Cuts at: 5228 5228
Size: 6499
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6499
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6499
AlwNI CAG_nnn'CTG
Cuts at: 2409 3172 3313 3712 4242 5814 2409
Size: 763 141 399 530 1572 3094
Fragments arranged by size:
3094 1572 763 530 399 141
ApaI G_GGCC'C
Cuts at: 2553 2782 3135 2553
Size: 229 353 5917
Fragments arranged by size:
5917 353 229
ApaLI G'TGCA_C
Cuts at: 3114 3700 4556 5712 3114
Size: 586 856 1156 3901
Fragments arranged by size:
3901 1156 856 586
ApoI r'AATT_y
Cuts at: 1493 3808 4034 4186 6295 1493
Size: 2315 226 152 2109 1697
Fragments arranged by size:
2315 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5053 160
Size: 872 4021 1606
Fragments arranged by size:
4021 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6499
AvaI C'yCGr_G
Cuts at: 1870 1943 2123 2731 3683 3824 1870
Size: 73 180 608 952 141 4545
Fragments arranged by size:
4545 952 608 180 141 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2736 2769 2736
Size: 33 6466
BamHI G'GATC_C
Cuts at: 3829 4196 3829
Size: 367 6132
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2704 3748 3820 5275 618
Size: 325 217 627 917 1044 72 1455 1842
Fragments arranged by size:
1842 1455 1044 917 627 325 217 72
BanII G_rGCy'C
Cuts at: 1391 2553 2621 2732 2782 3135 3342 3642 3818
Size: 1162 68 111 50 353 207 300 176
Cuts at: 3818 1391
Size: 4072
Fragments arranged by size:
4072 1162 353 300 207 176 111 68
50
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5528
BbvCI CC'TCA_GC
Cuts at: 2242 2278 2242
Size: 36 6463
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2940 2974 3488 3522 4690
Size: 34 50 34 919 34 514 34 1168
Cuts at: 4690 4724 1903
Size: 34 3678
Fragments arranged by size:
3678 1168 919 514 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2024 2730 2737 3470 4391 5601 2024
Size: 706 7 733 921 1210 2922
Fragments arranged by size:
2922 1210 921 733 706 7
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6499
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5110 136
Size: 107 122 71 631 605 3438 1525
Fragments arranged by size:
3438 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3774 6494 3774
Size: 2720 3779
BlpI GC'TnA_GC
Cuts at: 3288 3916 3288
Size: 628 5871
Bme1580I G_kGCm'C
Cuts at: 1790 2553 2782 3118 3135 3433 3704 3753 4560
Size: 763 229 336 17 298 271 49 807
Cuts at: 4560 5716 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 807 763 336 298 271 229
49 17
BmgBI CAC'GTC
Cuts at: 1318 3665 1318
Size: 2347 4152
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2354 4230 5188
Size: 416 53 83 399 24 937 1876 958
Cuts at: 5188 442
Size: 1753
Fragments arranged by size:
1876 1753 958 937 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6499
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2365 2547 3266 3341 3356 3758 5159 1239
Size: 1126 182 719 75 15 402 1401 2579
Fragments arranged by size:
2579 1401 1126 719 402 182 75 15
Bpu10I CC'TnA_GC
Cuts at: 2242 2278 2351 3400 2242
Size: 36 73 1049 5341
Fragments arranged by size:
5341 1049 73 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2744 3457 3716 4624 5489 5787 2744
Size: 713 259 908 865 298 3456
Fragments arranged by size:
3456 908 865 713 298 259
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5162 882
Size: 617 3663 2219
Fragments arranged by size:
3663 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3619 4290 493
Size: 711 2415 671 2702
Fragments arranged by size:
2702 2415 711 671
BsaBI GATnn'nnATC
Cuts at: 6493 6493
Size: 6499
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4306 4688 5323 275
Size: 53 83 186 936 2773 382 635 1451
Fragments arranged by size:
2773 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3236 4932 5330 5604 5751 6162 3236
Size: 1696 398 274 147 411 3573
Fragments arranged by size:
3573 1696 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3218 3248 1178
Size: 30 165 30 1815 30 4429
Fragments arranged by size:
4429 1815 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2230 2239 2242 2251 3086 3593 1186
Size: 1044 9 3 9 835 507 4092
Fragments arranged by size:
4092 1044 835 507 9 9 3
BseYI C'CCAG_C
Cuts at: 2156 2435 2451 2492 2597 2603 2656 3134 3203
Size: 279 16 41 105 6 53 478 69
Cuts at: 3203 3642 5702 6198 2156
Size: 439 2060 496 2457
Fragments arranged by size:
2457 2060 496 478 439 279 105 69
53 41 16 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2909 3467 1163
Size: 573 1173 558 4195
Fragments arranged by size:
4195 1173 573 558
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3547 3949 4710 5738 1540
Size: 275 186 1546 402 761 1028 2301
Fragments arranged by size:
2301 1546 1028 761 402 275 186
BsiHKAI G_wGCw'C
Cuts at: 3118 3342 3618 3704 4560 4645 5716 3118
Size: 224 276 86 856 85 1071 3901
Fragments arranged by size:
3901 1071 856 276 224 86 85
BsmI GAATG_Cn'
Cuts at: 4010 4103 4010
Size: 93 6406
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2553 2621 2732 2782 3118 3135 3342
Size: 399 763 68 111 50 336 17 207
Cuts at: 3342 3433 3618 3642 3704 3753 3818 4560 4645
Size: 91 185 24 62 49 65 742 85
Cuts at: 4645 5716 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 763 742 399 336 207 185
111 91 85 68 65 62 50 49
24 17
BspEI T'CCGG_A
Cuts at: 6162 6162
Size: 6499
BspHI T'CATG_A
Cuts at: 4388 6118 4388
Size: 1730 4769
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3237 3854 844
Size: 2393 617 3489
Fragments arranged by size:
3489 2393 617
BsrBI CCG'CTC
Cuts at: 1394 1823 2355 2775 4386 1394
Size: 429 532 420 1611 3507
Fragments arranged by size:
3507 1611 532 429 420
BsrDI GCAATG_nn'
Cuts at: 65 1248 4994 5168 65
Size: 1183 3746 174 1396
Fragments arranged by size:
3746 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2591 3045 3298 3417 3546 5143 5330
Size: 110 781 454 253 119 129 1597 187
Cuts at: 5330 1700
Size: 2869
Fragments arranged by size:
2869 1597 781 454 253 187 129 119
110
BsrGI T'GTAC_A
Cuts at: 96 3211 96
Size: 3115 3384
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6499
BssSI C'ACGA_G
Cuts at: 4559 5571 4559
Size: 1012 5487
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2409 3490 3556 794
Size: 1615 1081 66 3737
Fragments arranged by size:
3737 1615 1081 66
BstBI TT'CG_AA
Cuts at: 5303 5303
Size: 6499
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6499
BstXI CCAn_nnnn'nTGG
Cuts at: 2113 2840 2113
Size: 727 5772
BstYI r'GATC_y
Cuts at: 1972 3273 3774 3829 4196 4581 4598 6050 6136
Size: 1301 501 55 367 385 17 1452 86
Cuts at: 6136 6148 6494 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1301 501 385 367 346 86
55 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2106 3433 3566 513
Size: 552 1041 1327 133 3446
Fragments arranged by size:
3446 1327 1041 552 133
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3580 487
Size: 683 56 2354 3406
Fragments arranged by size:
3406 2354 683 56
BtsI GCAGTG_nn'
Cuts at: 2145 3138 3431 4015 4809 4829 2145
Size: 993 293 584 794 20 3815
Fragments arranged by size:
3815 993 794 584 293 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6499
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6464
DraI TTT'AAA
Cuts at: 3803 3803
Size: 6499
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5506 809
Size: 507 4190 1802
Fragments arranged by size:
4190 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2115 2479 3946
Size: 54 1425 50 275 18 285 364 1467
Cuts at: 3946 4835 6464 8
Size: 889 1629 43
Fragments arranged by size:
1629 1467 1425 889 364 285 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3946 1537
Size: 275 2134 4090
Fragments arranged by size:
4090 2134 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3347 4429 1353
Size: 1994 1082 3423
Fragments arranged by size:
3423 1994 1082
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3281 3341 5086 5460 5606 387
Size: 1064 138 1692 60 1745 374 146 1280
Fragments arranged by size:
1745 1692 1280 1064 374 146 138 60
EcoICRI GAG'CTC
Cuts at: 3340 3340
Size: 6499
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2146 2365 2547 2563 2645 3050 3266
Size: 393 514 219 182 16 82 405 216
Cuts at: 3266 3341 3356 3629 3758 4562 5159 5946 6214
Size: 75 15 273 129 804 597 787 268
Cuts at: 6214 1239
Size: 1524
Fragments arranged by size:
1524 804 787 597 514 405 393 273
268 219 216 182 129 82 75 16
15
EcoNI CCTnn'n_nnAGG
Cuts at: 3472 3472
Size: 6499
EcoO109I rG'GnC_Cy
Cuts at: 2051 2371 2550 2778 2779 3131 3310 3477 3709
Size: 320 179 228 1 352 179 167 232
Cuts at: 3709 2051
Size: 4841
Fragments arranged by size:
4841 352 320 232 228 179 179 167
1
EcoRI G'AATT_C
Cuts at: 3808 4186 3808
Size: 378 6121
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2890 3409 2890
Size: 519 5980
FspI TGC'GCA
Cuts at: 5005 5005
Size: 6499
HaeII r_GCGC'y
Cuts at: 1678 5646 1678
Size: 3968 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2360 2392 3218 3250 5140
Size: 32 135 32 788 32 826 32 1890
Cuts at: 5140 5172 5214 5246 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1890 826 788 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3843 4089 669
Size: 988 281 1905 246 3079
Fragments arranged by size:
3079 1905 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6499
HpaI GTT'AAC
Cuts at: 4089 4089
Size: 6499
KpnI G_GTAC'C
Cuts at: 3824 3824
Size: 6499
MfeI C'AATT_G
Cuts at: 4098 4098
Size: 6499
MluI A'CGCG_T
Cuts at: 6375 6375
Size: 6499
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5613 5797 1305
Size: 4308 184 2007
Fragments arranged by size:
4308 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2117 10
Size: 54 1425 343 285 4392
Fragments arranged by size:
4392 1425 343 285 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2171 2841 4457 4816 4975 518
Size: 648 1005 670 1616 359 159 2042
Fragments arranged by size:
2042 1616 1005 670 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2138 2215 2533 2656 2802 2958 3568 3603
Size: 532 77 318 123 146 156 610 35
Cuts at: 3603 3626 3654 3915 4592 5740 5985 1606
Size: 23 28 261 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 610 532 318 261 245
156 146 123 77 35 28 23
NaeI GCC'GGC
Cuts at: 3047 3047
Size: 6499
NcoI C'CATG_G
Cuts at: 513 1065 2106 513
Size: 552 1041 4906
Fragments arranged by size:
4906 1041 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6499
NgoMIV G'CCGG_C
Cuts at: 3045 3045
Size: 6499
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6499
NotI GC'GGCC_GC
Cuts at: 3946 3946
Size: 6499
NspI r_CATG'y
Cuts at: 1132 1229 2856 3857 5402 1132
Size: 97 1627 1001 1545 2229
Fragments arranged by size:
2229 1627 1545 1001 97
PasI CC'CwG_GG
Cuts at: 1348 2463 3706 1348
Size: 1115 1243 4141
Fragments arranged by size:
4141 1243 1115
PciI A'CATG_T
Cuts at: 2852 5398 2852
Size: 2546 3953
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2656 3172 1108
Size: 1548 516 4435
Fragments arranged by size:
4435 1548 516
PfoI T'CCnGG_A
Cuts at: 2047 3165 3333 2047
Size: 1118 168 5213
Fragments arranged by size:
5213 1118 168
PmeI GTTT'AAAC
Cuts at: 3803 3803
Size: 6499
PmlI CAC'GTG
Cuts at: 3619 3619
Size: 6499
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4568 4600 6081 6113 4568
Size: 32 1481 32 4954
Fragments arranged by size:
4954 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2051 3310 3477 2051
Size: 1259 167 5073
Fragments arranged by size:
5073 1259 167
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6499
PsiI TTA'TAA
Cuts at: 4069 4069
Size: 6499
PspOMI G'GGCC_C
Cuts at: 2549 2778 3131 2549
Size: 229 353 5917
Fragments arranged by size:
5917 353 229
PstI C_TGCA'G
Cuts at: 830 3426 3561 3588 3717 3851 830
Size: 2596 135 27 129 134 3478
Fragments arranged by size:
3478 2596 135 134 129 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6499
PvuII CAG'CTG
Cuts at: 2215 2533 2656 3603 3626 3654 2215
Size: 318 123 947 23 28 5060
Fragments arranged by size:
5060 947 318 123 28 23
SacI G_AGCT'C
Cuts at: 3342 3342
Size: 6499
SacII CC_GC'GG
Cuts at: 3569 3569
Size: 6499
SalI G'TCGA_C
Cuts at: 1936 3841 1936
Size: 1905 4594
SanDI GG'GwC_CC
Cuts at: 2051 2051
Size: 6499
SapI GCTCTTCn'nnn_
Cuts at: 3347 3347
Size: 6499
SbfI CC_TGCA'GG
Cuts at: 3851 3851
Size: 6499
ScaI AGT'ACT
Cuts at: 4747 6459 4747
Size: 1712 4787
SfcI C'TryA_G
Cuts at: 826 1045 3422 3557 3584 3713 3847 4982 5663
Size: 219 2377 135 27 129 134 1135 681
Cuts at: 5663 5854 826
Size: 191 1471
Fragments arranged by size:
2377 1471 1135 681 219 191 135 134
129 27
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6499
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6499
SmaI CCC'GGG
Cuts at: 1872 3826 1872
Size: 1954 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2723 3472 3731 4603 5504 5766
Size: 197 926 780 749 259 872 901 262
Cuts at: 5766 820
Size: 1553
Fragments arranged by size:
1553 926 901 872 780 749 262 259
197
SnaBI TAC'GTA
Cuts at: 493 4290 493
Size: 3797 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6499
SphI G_CATG'C
Cuts at: 1132 3857 1132
Size: 2725 3774
SspI AAT'ATT
Cuts at: 5 52 4423 5
Size: 47 4371 2081
Fragments arranged by size:
4371 2081 47
StuI AGG'CCT
Cuts at: 1517 2511 3405 1517
Size: 994 894 4611
Fragments arranged by size:
4611 994 894
StyI C'CwwG_G
Cuts at: 513 1065 2106 2763 3938 513
Size: 552 1041 657 1175 3074
Fragments arranged by size:
3074 1175 1041 657 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2663 2722 3719 3775 4526 4543 4696
Size: 1255 1083 59 997 56 751 17 153
Cuts at: 4696 4881 5326 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1083 997 751 445 185 153
59 56 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2904 3211 4745
Size: 274 80 33 51 1443 927 307 1534
Cuts at: 4745 6457 96
Size: 1712 138
Fragments arranged by size:
1712 1534 1443 927 307 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6459 6491 6459
Size: 32 6467
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6499
XbaI T'CTAG_A
Cuts at: 3835 3835
Size: 6499
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2024 2110 2605 2653 2024
Size: 86 495 48 5870
Fragments arranged by size:
5870 495 86 48
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6499
XmaI C'CCGG_G
Cuts at: 1870 3824 1870
Size: 1954 4545
XmnI GAAnn'nnTTC
Cuts at: 2630 4628 2630
Size: 1998 4501
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4307 276
Size: 53 83 186 936 2773 2468
Fragments arranged by size:
2773 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PspXI PsrI RsrII
SexAI SfiI SfoI SrfI SwaI