Restriction map for
pFN21AE1383
(Circular) (Six-base) MAPSORT of: pFN21AE1383.seq Check: 197 from: 1 to: 6772
pFN21AE1383 6772 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2413 2413
Size: 6772
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4582 278
Size: 53 83 186 936 3046 2468
Fragments arranged by size:
3046 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2946 4115 1937
Size: 1009 1169 4594
Fragments arranged by size:
4594 1169 1009
Acc65I G'GTAC_C
Cuts at: 2196 4093 2196
Size: 1897 4875
AclI AA'CG_TT
Cuts at: 1563 4899 5272 1563
Size: 3336 373 3063
Fragments arranged by size:
3336 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2560 2978 4835 6219 6487 1632
Size: 928 418 1857 1384 268 1917
Fragments arranged by size:
1917 1857 1384 928 418 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6575
AflIII A'CryG_T
Cuts at: 1203 2484 2781 3696 5671 6648 1203
Size: 1281 297 915 1975 977 1327
Fragments arranged by size:
1975 1327 1281 977 915 297
AgeI A'CCGG_T
Cuts at: 5603 5603
Size: 6772
AhdI GACnn_n'nnGTC
Cuts at: 5501 5501
Size: 6772
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6772
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2147 3878 1184
Size: 963 1731 4078
Fragments arranged by size:
4078 1731 963
AlwNI CAG_nnn'CTG
Cuts at: 2191 2879 3575 3802 3973 4515 6087 2191
Size: 688 696 227 171 542 1572 2876
Fragments arranged by size:
2876 1572 696 688 542 227 171
ApaLI G'TGCA_C
Cuts at: 4829 5985 4829
Size: 1156 5616
ApoI r'AATT_y
Cuts at: 1493 2255 2495 4081 4307 4459 6568 1493
Size: 762 240 1586 226 152 2109 1697
Fragments arranged by size:
2109 1697 1586 762 240 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5326 160
Size: 872 4294 1606
Fragments arranged by size:
4294 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6772
AvaI C'yCGr_G
Cuts at: 1870 1943 2108 3767 4097 1870
Size: 73 165 1659 330 4545
Fragments arranged by size:
4545 1659 330 165 73
BamHI G'GATC_C
Cuts at: 4102 4469 4102
Size: 367 6405
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2196 2425 2625 3059 3985
Size: 325 217 627 409 229 200 434 926
Cuts at: 3985 4036 4093 5548 618
Size: 51 57 1455 1842
Fragments arranged by size:
1842 1455 926 627 434 409 325 229
217 200 57 51
BanII G_rGCy'C
Cuts at: 1391 2115 2550 3337 3356 3519 4091 1391
Size: 724 435 787 19 163 572 4072
Fragments arranged by size:
4072 787 724 572 435 163 19
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2860 3379 3571 3663 928
Size: 971 961 519 192 92 4037
Fragments arranged by size:
4037 971 961 519 192 92
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2199 2233 3098 3132 4963 4997 1903
Size: 34 262 34 865 34 1831 34 3678
Fragments arranged by size:
3678 1831 865 262 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4664 5874 4664
Size: 1210 5562
BclI T'GATC_A
Cuts at: 1548 3508 1548
Size: 1960 4812
BfrBI ATG'CAT
Cuts at: 3688 3688
Size: 6772
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2075 5383 136
Size: 107 122 71 631 605 403 3308 1525
Fragments arranged by size:
3308 1525 631 605 403 122 107 71
BglII A'GATC_T
Cuts at: 2175 2325 6767 2175
Size: 150 4442 2180
Fragments arranged by size:
4442 2180 150
BlpI GC'TnA_GC
Cuts at: 2804 4028 4189 2804
Size: 1224 161 5387
Fragments arranged by size:
5387 1224 161
Bme1580I G_kGCm'C
Cuts at: 1790 3062 3168 3990 4833 5989 1790
Size: 1272 106 822 843 1156 2573
Fragments arranged by size:
2573 1272 1156 843 822 106
BmgBI CAC'GTC
Cuts at: 1318 2035 2485 1318
Size: 717 450 5605
Fragments arranged by size:
5605 717 450
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2757 3721 4503
Size: 416 53 83 399 24 1340 964 782
Cuts at: 4503 5461 442
Size: 958 1753
Fragments arranged by size:
1753 1340 964 958 782 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6772
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2036 2165 2252 2309 2939 3299 3632 5432
Size: 797 129 87 57 630 360 333 1800
Cuts at: 5432 1239
Size: 2579
Fragments arranged by size:
2579 1800 797 630 360 333 129 87
57
Bpu10I CC'TnA_GC
Cuts at: 3336 3918 3336
Size: 582 6190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3854 4897 5762 6060 3854
Size: 1043 865 298 4566
Fragments arranged by size:
4566 1043 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2696 5435 882
Size: 617 1197 2739 2219
Fragments arranged by size:
2739 2219 1197 617
BsaAI yAC'GTr
Cuts at: 493 1204 2995 4563 493
Size: 711 1791 1568 2702
Fragments arranged by size:
2702 1791 1568 711
BsaBI GATnn'nnATC
Cuts at: 6766 6766
Size: 6772
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3447 3951 4579 4961
Size: 53 83 186 936 1914 504 628 382
Cuts at: 4961 5596 275
Size: 635 1451
Fragments arranged by size:
1914 1451 936 635 628 504 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2431 3079 3306 5205 5603 5877 6024 6435 2431
Size: 648 227 1899 398 274 147 411 2768
Fragments arranged by size:
2768 1899 648 411 398 274 227 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2837 2867 3239 3269 3446
Size: 30 165 30 1434 30 372 30 177
Cuts at: 3446 3476 3809 3839 3842 3872 1178
Size: 30 333 30 3 30 4078
Fragments arranged by size:
4078 1434 372 333 177 165 30 30
30 30 30 30 30 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2249 2279 2312 2315 3488 3830 3848 3857
Size: 1063 30 33 3 1173 342 18 9
Cuts at: 3857 1186
Size: 4101
Fragments arranged by size:
4101 1173 1063 342 33 30 18 9
3
BseYI C'CCAG_C
Cuts at: 2114 2191 2856 3643 3966 5975 6471 2114
Size: 77 665 787 323 2009 496 2415
Fragments arranged by size:
2415 2009 787 665 496 323 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2825 3947 3998 1163
Size: 573 1089 1122 51 3937
Fragments arranged by size:
3937 1122 1089 573 51
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2079 2901 3214 3444 4222 4983
Size: 275 186 78 822 313 230 778 761
Cuts at: 4983 6011 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 822 778 761 313 275 230
186 78
BsiHKAI G_wGCw'C
Cuts at: 2550 2925 3519 4833 4918 5989 2550
Size: 375 594 1314 85 1071 3333
Fragments arranged by size:
3333 1314 1071 594 375 85
BsmI GAATG_Cn'
Cuts at: 4283 4376 4283
Size: 93 6679
BsmBI CGTCTCn'nnnn_
Cuts at: 2285 3830 2285
Size: 1545 5227
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2115 2550 2925 3062 3168 3337 3356
Size: 399 325 435 375 137 106 169 19
Cuts at: 3356 3519 3990 4091 4833 4918 5989 1391
Size: 163 471 101 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 471 435 399 375 325
169 163 137 106 101 85 19
BspEI T'CCGG_A
Cuts at: 2431 3079 6435 2431
Size: 648 3356 2768
Fragments arranged by size:
3356 2768 648
BspHI T'CATG_A
Cuts at: 2343 2823 4661 6391 2343
Size: 480 1838 1730 2724
Fragments arranged by size:
2724 1838 1730 480
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2208 2413 2847 4127 844
Size: 1364 205 434 1280 3489
Fragments arranged by size:
3489 1364 1280 434 205
BsrBI CCG'CTC
Cuts at: 1394 1823 3366 3550 4659 1394
Size: 429 1543 184 1109 3507
Fragments arranged by size:
3507 1543 1109 429 184
BsrDI GCAATG_nn'
Cuts at: 65 1248 5267 5441 65
Size: 1183 4019 174 1396
Fragments arranged by size:
4019 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2074 3606 5416 5603 1700
Size: 110 264 1532 1810 187 2869
Fragments arranged by size:
2869 1810 1532 264 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6772
BssHII G'CGCG_C
Cuts at: 1925 2081 2083 1925
Size: 156 2 6614
Fragments arranged by size:
6614 156 2
BssSI C'ACGA_G
Cuts at: 3423 4832 5844 3423
Size: 1409 1012 4351
Fragments arranged by size:
4351 1409 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3678 3802 3976 3985 794
Size: 2884 124 174 9 3581
Fragments arranged by size:
3581 2884 174 124 9
BstBI TT'CG_AA
Cuts at: 5576 5576
Size: 6772
BstEII G'GTnAC_C
Cuts at: 1184 2161 1184
Size: 977 5795
BstXI CCAn_nnnn'nTGG
Cuts at: 2248 2248
Size: 6772
BstYI r'GATC_y
Cuts at: 1972 2175 2325 2434 3000 3819 3873 4102 4469
Size: 203 150 109 566 819 54 229 367
Cuts at: 4469 4854 4871 6323 6409 6421 6767 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 819 566 385 367 346 229
203 150 109 86 54 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2052 3064 3234 3810 3877 4017
Size: 552 939 48 1012 170 576 67 140
Cuts at: 4017 513
Size: 3268
Fragments arranged by size:
3268 1012 939 576 552 170 140 67
48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2570 487
Size: 683 56 1344 4689
Fragments arranged by size:
4689 1344 683 56
BtsI GCAGTG_nn'
Cuts at: 2131 3121 3908 3935 4063 4288 5082 5102 2131
Size: 990 787 27 128 225 794 20 3801
Fragments arranged by size:
3801 990 794 787 225 128 27 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6772
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6737
DraI TTT'AAA
Cuts at: 4076 4076
Size: 6772
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5779 809
Size: 507 4463 1802
Fragments arranged by size:
4463 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2049 2076
Size: 54 1425 50 275 18 198 21 27
Cuts at: 2076 2898 2989 3231 3441 4219 5108 6737 8
Size: 822 91 242 210 778 889 1629 43
Fragments arranged by size:
1629 1425 889 822 778 275 242 210
198 91 54 50 43 27 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 2076 2898 3441 4219 1537
Size: 275 264 822 543 778 4090
Fragments arranged by size:
4090 822 778 543 275 264
EarI CTCTTCn'nnn_
Cuts at: 1353 2054 2210 2375 2444 2451 3152 4702 1353
Size: 701 156 165 69 7 701 1550 3423
Fragments arranged by size:
3423 1550 701 701 165 156 69 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 5359 5733 5879 387
Size: 1064 138 435 3335 374 146 1280
Fragments arranged by size:
3335 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 2548 3517 2548
Size: 969 5803
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2165 2252 2309 2560 2939 2978
Size: 393 404 129 87 57 251 379 39
Cuts at: 2978 3299 3632 4835 5432 6219 6487 1239
Size: 321 333 1203 597 787 268 1524
Fragments arranged by size:
1524 1203 787 597 404 393 379 333
321 268 251 129 87 57 39
EcoNI CCTnn'n_nnAGG
Cuts at: 2234 3092 3278 3671 2234
Size: 858 186 393 5335
Fragments arranged by size:
5335 858 393 186
EcoO109I rG'GnC_Cy
Cuts at: 2244 2836 2953 3085 3790 2244
Size: 592 117 132 705 5226
Fragments arranged by size:
5226 705 592 132 117
EcoRI G'AATT_C
Cuts at: 2255 4081 4459 2255
Size: 1826 378 4568
Fragments arranged by size:
4568 1826 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2824 2824
Size: 6772
FspI TGC'GCA
Cuts at: 5278 5278
Size: 6772
HaeII r_GCGC'y
Cuts at: 1678 5919 1678
Size: 4241 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3113 3145 3239 3271 3446
Size: 32 135 32 1541 32 94 32 175
Cuts at: 3446 3478 3809 3841 5413 5445 5487 5519 1373
Size: 32 331 32 1572 32 42 32 2626
Fragments arranged by size:
2626 1572 1541 331 175 135 94 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3304 4116 4362 669
Size: 988 281 1366 812 246 3079
Fragments arranged by size:
3079 1366 988 812 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6772
HpaI GTT'AAC
Cuts at: 4362 4362
Size: 6772
KpnI G_GTAC'C
Cuts at: 2200 4097 2200
Size: 1897 4875
MfeI C'AATT_G
Cuts at: 4371 4371
Size: 6772
MluI A'CGCG_T
Cuts at: 2781 6648 2781
Size: 3867 2905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5886 6070 1305
Size: 4581 184 2007
Fragments arranged by size:
4581 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2051 2991 10
Size: 54 1425 343 219 940 3791
Fragments arranged by size:
3791 1425 940 343 219 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2687 4730 5089 5248 518
Size: 648 1521 2043 359 159 2042
Fragments arranged by size:
2043 2042 1521 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3412 3643 3736 3879 3973 3982 4042 4188
Size: 1806 231 93 143 94 9 60 146
Cuts at: 4188 4865 6013 6258 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1806 1148 677 245 231 146 143
94 93 60 9
NaeI GCC'GGC
Cuts at: 2076 2076
Size: 6772
NcoI C'CATG_G
Cuts at: 513 1065 2004 2052 3810 513
Size: 552 939 48 1758 3475
Fragments arranged by size:
3475 1758 939 552 48
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6772
NgoMIV G'CCGG_C
Cuts at: 2074 2074
Size: 6772
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6772
NotI GC'GGCC_GC
Cuts at: 4219 4219
Size: 6772
NsiI A_TGCA'T
Cuts at: 3690 3690
Size: 6772
NspI r_CATG'y
Cuts at: 1132 1229 2399 2776 2887 2929 3145 3700 4130
Size: 97 1170 377 111 42 216 555 430
Cuts at: 4130 5675 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 1170 555 430 377 216 111
97 42
PasI CC'CwG_GG
Cuts at: 1348 3667 1348
Size: 2319 4453
PciI A'CATG_T
Cuts at: 3696 5671 3696
Size: 1975 4797
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2191 3829 3973 1108
Size: 1083 1638 144 3907
Fragments arranged by size:
3907 1638 1083 144
PfoI T'CCnGG_A
Cuts at: 2814 2814
Size: 6772
PmeI GTTT'AAAC
Cuts at: 4076 4076
Size: 6772
PmlI CAC'GTG
Cuts at: 2995 2995
Size: 6772
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4841 4873 6354 6386 4841
Size: 32 1481 32 5227
Fragments arranged by size:
5227 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2836 2953 3085 2836
Size: 117 132 6523
Fragments arranged by size:
6523 132 117
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6772
PsiI TTA'TAA
Cuts at: 4342 4342
Size: 6772
PspXI vC'TCGA_Gb
Cuts at: 3767 3767
Size: 6772
PstI C_TGCA'G
Cuts at: 830 3093 3294 4124 830
Size: 2263 201 830 3478
Fragments arranged by size:
3478 2263 830 201
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6772
PvuII CAG'CTG
Cuts at: 3643 3973 3643
Size: 330 6442
SacI G_AGCT'C
Cuts at: 2550 3519 2550
Size: 969 5803
SacII CC_GC'GG
Cuts at: 3880 3880
Size: 6772
SalI G'TCGA_C
Cuts at: 1936 4114 1936
Size: 2178 4594
SapI GCTCTTCn'nnn_
Cuts at: 2054 2210 3152 2054
Size: 156 942 5674
Fragments arranged by size:
5674 942 156
SbfI CC_TGCA'GG
Cuts at: 4124 4124
Size: 6772
ScaI AGT'ACT
Cuts at: 5020 6732 5020
Size: 1712 5060
SfcI C'TryA_G
Cuts at: 826 1045 3089 3290 4120 5255 5936 6127 826
Size: 219 2044 201 830 1135 681 191 1471
Fragments arranged by size:
2044 1471 1135 830 681 219 201 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2075 2075
Size: 6772
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6772
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6772
SmaI CCC'GGG
Cuts at: 1872 4099 1872
Size: 2227 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3767 3869 4876 5777 6039 820
Size: 197 926 1824 102 1007 901 262 1553
Fragments arranged by size:
1824 1553 1007 926 901 262 197 102
SnaBI TAC'GTA
Cuts at: 493 4563 493
Size: 4070 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6772
SphI G_CATG'C
Cuts at: 1132 3145 4130 1132
Size: 2013 985 3774
Fragments arranged by size:
3774 2013 985
SspI AAT'ATT
Cuts at: 5 52 4696 5
Size: 47 4644 2081
Fragments arranged by size:
4644 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6772
StyI C'CwwG_G
Cuts at: 513 1065 2004 2052 2247 2401 3810 4211 513
Size: 552 939 48 195 154 1409 401 3074
Fragments arranged by size:
3074 1409 939 552 401 195 154 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2613 3077 4799 4816 4969 5154 5599
Size: 1255 1033 464 1722 17 153 185 445
Cuts at: 5599 325
Size: 1498
Fragments arranged by size:
1722 1498 1255 1033 464 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5018 6730 96
Size: 274 80 33 51 1443 3041 1712 138
Fragments arranged by size:
3041 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3842 3874 6732 6764 3842
Size: 32 2858 32 3850
Fragments arranged by size:
3850 2858 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6772
XbaI T'CTAG_A
Cuts at: 4108 4108
Size: 6772
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2248 2665 2974 2011
Size: 237 417 309 5809
Fragments arranged by size:
5809 417 309 237
XhoI C'TCGA_G
Cuts at: 1943 3767 1943
Size: 1824 4948
XmaI C'CCGG_G
Cuts at: 1870 4097 1870
Size: 2227 4545
XmnI GAAnn'nnTTC
Cuts at: 2573 3890 4901 2573
Size: 1317 1011 4444
Fragments arranged by size:
4444 1317 1011
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4580 276
Size: 53 83 186 936 3046 2468
Fragments arranged by size:
3046 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI
KasI NarI NruI PacI PspOMI PsrI RsrII SanDI
SexAI SfoI SrfI SwaI