Restriction map for
pFN21AE1381
(Circular) (Six-base) MAPSORT of: pFN21AE1381.seq Check: 9019 from: 1 to: 7054
pFN21AE1381 7054 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2791 3082 2791
Size: 291 6763
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4864 278
Size: 53 83 186 936 3328 2468
Fragments arranged by size:
3328 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4104 4397 1937
Size: 2167 293 4594
Fragments arranged by size:
4594 2167 293
Acc65I G'GTAC_C
Cuts at: 2886 4375 2886
Size: 1489 5565
AclI AA'CG_TT
Cuts at: 1563 5181 5554 1563
Size: 3618 373 3063
Fragments arranged by size:
3618 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2352 2642 4293 5117 6501 6769 1632
Size: 720 290 1651 824 1384 268 1917
Fragments arranged by size:
1917 1651 1384 824 720 290 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6857
AflIII A'CryG_T
Cuts at: 1203 3292 3687 5953 6930 1203
Size: 2089 395 2266 977 1327
Fragments arranged by size:
2266 2089 1327 977 395
AgeI A'CCGG_T
Cuts at: 5885 5885
Size: 7054
AhdI GACnn_n'nnGTC
Cuts at: 5783 5783
Size: 7054
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7054
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2342 1184
Size: 1158 5896
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2399 2431 2399
Size: 32 7022
AlwNI CAG_nnn'CTG
Cuts at: 2314 3199 3220 3499 3840 3918 4797 6369 2314
Size: 885 21 279 341 78 879 1572 2999
Fragments arranged by size:
2999 1572 885 879 341 279 78 21
ApaI G_GGCC'C
Cuts at: 2594 2709 3186 3232 3260 3427 3434 4069 2594
Size: 115 477 46 28 167 7 635 5579
Fragments arranged by size:
5579 635 477 167 115 46 28 7
ApaLI G'TGCA_C
Cuts at: 5111 6267 5111
Size: 1156 5898
ApoI r'AATT_y
Cuts at: 1493 2723 4363 4589 4741 6850 1493
Size: 1230 1640 226 152 2109 1697
Fragments arranged by size:
2109 1697 1640 1230 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5608 160
Size: 872 4576 1606
Fragments arranged by size:
4576 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7054
AvaI C'yCGr_G
Cuts at: 1870 1943 2266 2457 2692 2994 3185 3231 3420
Size: 73 323 191 235 302 191 46 189
Cuts at: 3420 4379 1870
Size: 959 4545
Fragments arranged by size:
4545 959 323 302 235 191 191 189
73 46
AvrII C'CTAG_G
Cuts at: 2701 2701
Size: 7054
BamHI G'GATC_C
Cuts at: 3904 4384 4751 3904
Size: 480 367 6207
Fragments arranged by size:
6207 480 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2073 2654 2886 2983 3079
Size: 325 217 627 286 581 232 97 96
Cuts at: 3079 3363 3608 4200 4375 5830 618
Size: 284 245 592 175 1455 1842
Fragments arranged by size:
1842 1455 627 592 581 325 286 284
245 232 217 175 97 96
BanII G_rGCy'C
Cuts at: 1391 2036 2047 2265 2381 2405 2412 2564 2594
Size: 645 11 218 116 24 7 152 30
Cuts at: 2594 2709 2879 3005 3186 3192 3232 3260 3427
Size: 115 170 126 181 6 40 28 167
Cuts at: 3427 3434 3708 3809 4069 4373 1391
Size: 7 274 101 260 304 4072
Fragments arranged by size:
4072 645 304 274 260 218 181 170
167 152 126 116 115 101 40 30
28 24 11 7 7 6
BbeI G_GCGC'C
Cuts at: 3367 3367
Size: 7054
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6083
BbvCI CC'TCA_GC
Cuts at: 2252 3402 3833 2252
Size: 1150 431 5473
Fragments arranged by size:
5473 1150 431
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5245 5279 1903
Size: 34 3308 34 3678
Fragments arranged by size:
3678 3308 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4946 6156 4946
Size: 1210 5844
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7054
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2155 2209 2385
Size: 107 122 71 631 605 483 54 176
Cuts at: 2385 3283 3337 3761 5665 136
Size: 898 54 424 1904 1525
Fragments arranged by size:
1904 1525 898 631 605 483 424 176
122 107 71 54 54
BglII A'GATC_T
Cuts at: 7049 7049
Size: 7054
BlpI GC'TnA_GC
Cuts at: 2583 3221 3632 4308 4471 2583
Size: 638 411 676 163 5166
Fragments arranged by size:
5166 676 638 411 163
Bme1580I G_kGCm'C
Cuts at: 1790 2076 2121 2594 2657 2709 3186 3232 3260
Size: 286 45 473 63 52 477 46 28
Cuts at: 3260 3427 3434 3945 4069 5115 6271 1790
Size: 167 7 511 124 1046 1156 2573
Fragments arranged by size:
2573 1156 1046 511 477 473 286 167
124 63 52 46 45 28 7
BmgBI CAC'GTC
Cuts at: 1318 2116 1318
Size: 798 6256
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2079 2600 3464
Size: 416 53 83 399 24 662 521 864
Cuts at: 3464 4785 5743 442
Size: 1321 958 1753
Fragments arranged by size:
1753 1321 958 864 662 521 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7054
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2551 2551
Size: 7054
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2037 2061 2341 2393 2546 2578 2619 2749
Size: 798 24 280 52 153 32 41 130
Cuts at: 2749 3320 3695 3722 3741 5714 1239
Size: 571 375 27 19 1973 2579
Fragments arranged by size:
2579 1973 798 571 375 280 153 130
52 41 32 27 24 19
Bpu10I CC'TnA_GC
Cuts at: 2252 3402 3455 3663 3833 2252
Size: 1150 53 208 170 5473
Fragments arranged by size:
5473 1150 208 170 53
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2622 3311 5179 6044 6342 2622
Size: 689 1868 865 298 3334
Fragments arranged by size:
3334 1868 865 689 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2753 4121 5717 882
Size: 617 1254 1368 1596 2219
Fragments arranged by size:
2219 1596 1368 1254 617
BsaAI yAC'GTr
Cuts at: 493 1204 4845 493
Size: 711 3641 2702
Fragments arranged by size:
3641 2702 711
BsaBI GATnn'nnATC
Cuts at: 7048 7048
Size: 7054
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2388 3364 4861 5243
Size: 53 83 186 936 855 976 1497 382
Cuts at: 5243 5878 275
Size: 635 1451
Fragments arranged by size:
1497 1451 976 936 855 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2657 3082 5487 5885 6159 6306 6717 2657
Size: 425 2405 398 274 147 411 2994
Fragments arranged by size:
2994 2405 425 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2399 2429 2592 2622 2939
Size: 30 165 30 996 30 163 30 317
Cuts at: 2939 2969 3668 3698 4184 4214 1178
Size: 30 699 30 486 30 4018
Fragments arranged by size:
4018 996 699 486 317 165 163 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2462 2554 2987 2990 3875 4163 4166 4205
Size: 1276 92 433 3 885 288 3 39
Cuts at: 4205 4364 1186
Size: 159 3876
Fragments arranged by size:
3876 1276 885 433 288 159 92 39
3 3
BseYI C'CCAG_C
Cuts at: 2048 2616 2828 2960 3004 3179 3253 3527 3707
Size: 568 212 132 44 175 74 274 180
Cuts at: 3707 3908 4002 4220 6257 6753 2048
Size: 201 94 218 2037 496 2349
Fragments arranged by size:
2349 2037 568 496 274 218 212 201
180 175 132 94 74 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2228 3173 3833 3904 1163
Size: 573 492 945 660 71 4313
Fragments arranged by size:
4313 945 660 573 492 71
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2159 2238 4504 5265 6293 1540
Size: 275 186 158 79 2266 761 1028 2301
Fragments arranged by size:
2301 2266 1028 761 275 186 158 79
BsiHKAI G_wGCw'C
Cuts at: 2412 2564 2879 3327 5115 5200 6271 2412
Size: 152 315 448 1788 85 1071 3195
Fragments arranged by size:
3195 1788 1071 448 315 152 85
BsmI GAATG_Cn'
Cuts at: 4565 4658 4565
Size: 93 6961
BsmBI CGTCTCn'nnnn_
Cuts at: 2359 2395 2359
Size: 36 7018
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2047 2076 2121 2265 2381 2405
Size: 399 246 11 29 45 144 116 24
Cuts at: 2405 2412 2564 2594 2657 2709 2879 3005 3186
Size: 7 152 30 63 52 170 126 181
Cuts at: 3186 3192 3232 3260 3327 3427 3434 3708 3809
Size: 6 40 28 67 100 7 274 101
Cuts at: 3809 3945 4069 4373 5115 5200 6271 1391
Size: 136 124 304 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 304 274 246 181
170 152 144 136 126 124 116 101
100 85 67 63 52 45 40 30
29 28 24 11 7 7 6
BspEI T'CCGG_A
Cuts at: 6717 6717
Size: 7054
BspHI T'CATG_A
Cuts at: 4943 6673 4943
Size: 1730 5324
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2791 3082 4409 844
Size: 1947 291 1327 3489
Fragments arranged by size:
3489 1947 1327 291
BsrBI CCG'CTC
Cuts at: 1394 1823 2842 3088 4941 1394
Size: 429 1019 246 1853 3507
Fragments arranged by size:
3507 1853 1019 429 246
BsrDI GCAATG_nn'
Cuts at: 65 1248 3308 3313 5549 5723 65
Size: 1183 2060 5 2236 174 1396
Fragments arranged by size:
2236 2060 1396 1183 174 5
BsrFI r'CCGG_y
Cuts at: 1700 1810 3366 5698 5885 1700
Size: 110 1556 2332 187 2869
Fragments arranged by size:
2869 2332 1556 187 110
BsrGI T'GTAC_A
Cuts at: 96 4029 96
Size: 3933 3121
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7054
BssSI C'ACGA_G
Cuts at: 2279 5114 6126 2279
Size: 2835 1012 3207
Fragments arranged by size:
3207 2835 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3918 794
Size: 3124 3930
BstBI TT'CG_AA
Cuts at: 5858 5858
Size: 7054
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7054
BstXI CCAn_nnnn'nTGG
Cuts at: 3934 4211 3934
Size: 277 6777
BstYI r'GATC_y
Cuts at: 1972 3904 4384 4751 5136 5153 6605 6691 6703
Size: 1932 480 367 385 17 1452 86 12
Cuts at: 6703 7049 1972
Size: 346 1977
Fragments arranged by size:
1977 1932 1452 480 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3581 3581
Size: 7054
BtgI C'CryG_G
Cuts at: 513 1065 2004 3138 513
Size: 552 939 1134 4429
Fragments arranged by size:
4429 1134 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6315
Fragments arranged by size:
6315 683 56
BtsI GCAGTG_nn'
Cuts at: 2240 2911 3868 3919 4165 4570 5364 5384 2240
Size: 671 957 51 246 405 794 20 3910
Fragments arranged by size:
3910 957 794 671 405 246 51 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7054
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7019
DraI TTT'AAA
Cuts at: 4358 4358
Size: 7054
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6061 809
Size: 507 4745 1802
Fragments arranged by size:
4745 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2156 2235 3936
Size: 54 1425 50 275 18 326 79 1701
Cuts at: 3936 4501 5390 7019 8
Size: 565 889 1629 43
Fragments arranged by size:
1701 1629 1425 889 565 326 275 79
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2156 2235 4501 1537
Size: 275 344 79 2266 4090
Fragments arranged by size:
4090 2266 344 275 79
EarI CTCTTCn'nnn_
Cuts at: 1353 4028 4984 1353
Size: 2675 956 3423
Fragments arranged by size:
3423 2675 956
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2910 3401 5641 6015 6161 387
Size: 1064 138 1321 491 2240 374 146 1280
Fragments arranged by size:
2240 1321 1280 1064 491 374 146 138
EcoICRI GAG'CTC
Cuts at: 2410 2562 2877 2410
Size: 152 315 6587
Fragments arranged by size:
6587 315 152
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2037 2061 2341 2352 2393 2546 2578
Size: 393 405 24 280 11 41 153 32
Cuts at: 2578 2619 2642 2749 3320 3695 3722 3741 4293
Size: 41 23 107 571 375 27 19 552
Cuts at: 4293 5117 5714 6501 6769 1239
Size: 824 597 787 268 1524
Fragments arranged by size:
1524 824 787 597 571 552 405 393
375 280 268 153 107 41 41 32
27 24 23 19 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2939 3401 3782 4031 2939
Size: 462 381 249 5962
Fragments arranged by size:
5962 462 381 249
EcoO109I rG'GnC_Cy
Cuts at: 2101 2311 2590 2698 2705 2991 3157 3257 3406
Size: 210 279 108 7 286 166 100 149
Cuts at: 3406 3430 3431 3447 3585 4065 4066 4301 2101
Size: 24 1 16 138 480 1 235 4854
Fragments arranged by size:
4854 480 286 279 235 210 166 149
138 108 100 24 16 7 1 1
EcoRI G'AATT_C
Cuts at: 4363 4741 4363
Size: 378 6676
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2752 2752
Size: 7054
FspI TGC'GCA
Cuts at: 5560 5560
Size: 7054
HaeII r_GCGC'y
Cuts at: 1678 2188 3060 3221 3367 3534 3829 3853 6201
Size: 510 872 161 146 167 295 24 2348
Cuts at: 6201 1678
Size: 2531
Fragments arranged by size:
2531 2348 872 510 295 167 161 146
24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2939 2971 3674 3706 5695
Size: 32 135 32 1367 32 703 32 1989
Cuts at: 5695 5727 5769 5801 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1989 1367 703 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4398 4644 669
Size: 988 281 2460 246 3079
Fragments arranged by size:
3079 2460 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2734 748
Size: 1986 5068
HpaI GTT'AAC
Cuts at: 4644 4644
Size: 7054
KasI G'GCGC_C
Cuts at: 3363 3363
Size: 7054
KpnI G_GTAC'C
Cuts at: 2890 4379 2890
Size: 1489 5565
MfeI C'AATT_G
Cuts at: 4653 4653
Size: 7054
MluI A'CGCG_T
Cuts at: 6930 6930
Size: 7054
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3459 3672 6168 6352 1305
Size: 2154 213 2496 184 2007
Fragments arranged by size:
2496 2154 2007 213 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5232
Fragments arranged by size:
5232 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5012 5371 5530 518
Size: 648 3846 359 159 2042
Fragments arranged by size:
3846 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2130 2155 2218 2234 2479 2587 2620 2860
Size: 524 25 63 16 245 108 33 240
Cuts at: 2860 3140 3148 3508 4470 5147 6295 6540 1606
Size: 280 8 360 962 677 1148 245 2120
Fragments arranged by size:
2120 1148 962 677 524 360 280 245
245 240 108 63 33 25 16 8
NaeI GCC'GGC
Cuts at: 3368 3368
Size: 7054
NarI GG'CG_CC
Cuts at: 3364 3364
Size: 7054
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5563
Fragments arranged by size:
5563 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7054
NgoMIV G'CCGG_C
Cuts at: 3366 3366
Size: 7054
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7054
NotI GC'GGCC_GC
Cuts at: 2235 4501 2235
Size: 2266 4788
NspI r_CATG'y
Cuts at: 1132 1229 2251 2323 3296 3691 4412 5957 1132
Size: 97 1022 72 973 395 721 1545 2229
Fragments arranged by size:
2229 1545 1022 973 721 395 97 72
PasI CC'CwG_GG
Cuts at: 1348 3388 3427 4061 4132 4239 1348
Size: 2040 39 634 71 107 4163
Fragments arranged by size:
4163 2040 634 107 71 39
PciI A'CATG_T
Cuts at: 3292 3687 5953 3292
Size: 395 2266 4393
Fragments arranged by size:
4393 2266 395
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2944 1108
Size: 1836 5218
PfoI T'CCnGG_A
Cuts at: 2038 2370 3297 2038
Size: 332 927 5795
Fragments arranged by size:
5795 927 332
PmeI GTTT'AAAC
Cuts at: 4358 4358
Size: 7054
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2399 2431 5123 5155 6636 6668 2399
Size: 32 2692 32 1481 32 2785
Fragments arranged by size:
2785 2692 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2101 2698 2991 3157 3447 3585 2101
Size: 597 293 166 290 138 5570
Fragments arranged by size:
5570 597 293 290 166 138
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7054
PsiI TTA'TAA
Cuts at: 4624 4624
Size: 7054
PspOMI G'GGCC_C
Cuts at: 2590 2705 3182 3228 3256 3423 3430 4065 2590
Size: 115 477 46 28 167 7 635 5579
Fragments arranged by size:
5579 635 477 167 115 46 28 7
PstI C_TGCA'G
Cuts at: 830 2217 2484 3204 3507 3555 4406 830
Size: 1387 267 720 303 48 851 3478
Fragments arranged by size:
3478 1387 851 720 303 267 48
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7054
PvuII CAG'CTG
Cuts at: 2218 2620 2860 3148 3508 2218
Size: 402 240 288 360 5764
Fragments arranged by size:
5764 402 360 288 240
SacI G_AGCT'C
Cuts at: 2412 2564 2879 2412
Size: 152 315 6587
Fragments arranged by size:
6587 315 152
SacII CC_GC'GG
Cuts at: 3141 3141
Size: 7054
SalI G'TCGA_C
Cuts at: 1936 4396 1936
Size: 2460 4594
SanDI GG'GwC_CC
Cuts at: 2991 3447 2991
Size: 456 6598
SbfI CC_TGCA'GG
Cuts at: 3555 4406 3555
Size: 851 6203
ScaI AGT'ACT
Cuts at: 5302 7014 5302
Size: 1712 5342
SfcI C'TryA_G
Cuts at: 826 1045 2213 2480 3200 3503 3551 4402 5537
Size: 219 1168 267 720 303 48 851 1135
Cuts at: 5537 6218 6409 826
Size: 681 191 1471
Fragments arranged by size:
1471 1168 1135 851 720 681 303 267
219 191 48
SfiI GGCCn_nnn'nGGCC
Cuts at: 2155 2155
Size: 7054
SfoI GGC'GCC
Cuts at: 3365 3365
Size: 7054
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7054
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7054
SmaI CCC'GGG
Cuts at: 1872 2268 2459 2694 2996 3233 3422 4381 1872
Size: 396 191 235 302 237 189 959 4545
Fragments arranged by size:
4545 959 396 302 237 235 191 189
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2637 3326 5158 6059 6321 820
Size: 197 926 694 689 1832 901 262 1553
Fragments arranged by size:
1832 1553 926 901 694 689 262 197
SnaBI TAC'GTA
Cuts at: 493 4845 493
Size: 4352 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7054
SphI G_CATG'C
Cuts at: 1132 2323 4412 1132
Size: 1191 2089 3774
Fragments arranged by size:
3774 2089 1191
SrfI GCCC'GGGC
Cuts at: 3233 3422 3233
Size: 189 6865
SspI AAT'ATT
Cuts at: 5 52 4978 5
Size: 47 4926 2081
Fragments arranged by size:
4926 2081 47
StuI AGG'CCT
Cuts at: 1517 3557 3953 1517
Size: 2040 396 4618
Fragments arranged by size:
4618 2040 396
StyI C'CwwG_G
Cuts at: 513 1065 2004 2394 2701 4493 513
Size: 552 939 390 307 1792 3074
Fragments arranged by size:
3074 1792 939 552 390 307
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2273 3036 3141 3541 3881 5081 5098
Size: 1255 693 763 105 400 340 1200 17
Cuts at: 5098 5251 5436 5881 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1200 763 693 445 400 340
185 153 105 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3684 4029 5300
Size: 274 80 33 51 1443 1707 345 1271
Cuts at: 5300 7012 96
Size: 1712 138
Fragments arranged by size:
1712 1707 1443 1271 345 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2592 2624 4184 4216 7014 7046 2592
Size: 32 1560 32 2798 32 2600
Fragments arranged by size:
2798 2600 1560 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2426 1316
Size: 1110 5944
XbaI T'CTAG_A
Cuts at: 4390 4390
Size: 7054
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2539 3176 2539
Size: 637 6417
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7054
XmaI C'CCGG_G
Cuts at: 1870 2266 2457 2692 2994 3231 3420 4379 1870
Size: 396 191 235 302 237 189 959 4545
Fragments arranged by size:
4545 959 396 302 237 235 191 189
XmnI GAAnn'nnTTC
Cuts at: 5183 5183
Size: 7054
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4862 276
Size: 53 83 186 936 3328 2468
Fragments arranged by size:
3328 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SanDI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BfrBI BsiWI BstZ17I DraIII EcoRV
FseI FspAI NruI NsiI PacI PmlI PspXI PsrI
RsrII SapI SexAI SwaI