Restriction map for
pFN21AE1380
(Circular) (Six-base) MAPSORT of: pFN21AE1380.seq Check: 9852 from: 1 to: 6679
pFN21AE1380 6679 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3238 3238
Size: 6679
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4489 278
Size: 53 83 186 936 2953 2468
Fragments arranged by size:
2953 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4022 1937
Size: 2085 4594
Acc65I G'GTAC_C
Cuts at: 4000 4000
Size: 6679
AclI AA'CG_TT
Cuts at: 1563 4806 5179 1563
Size: 3243 373 3063
Fragments arranged by size:
3243 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2084 3577 4742 6126 6394 1632
Size: 452 1493 1165 1384 268 1917
Fragments arranged by size:
1917 1493 1384 1165 452 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6482
AflIII A'CryG_T
Cuts at: 1203 2778 3854 5578 6555 1203
Size: 1575 1076 1724 977 1327
Fragments arranged by size:
1724 1575 1327 1076 977
AgeI A'CCGG_T
Cuts at: 5510 5510
Size: 6679
AhdI GACnn_n'nnGTC
Cuts at: 5408 5408
Size: 6679
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6679
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6679
AlwNI CAG_nnn'CTG
Cuts at: 2338 4422 5994 2338
Size: 2084 1572 3023
Fragments arranged by size:
3023 2084 1572
ApaI G_GGCC'C
Cuts at: 2114 2228 2468 2697 3817 2114
Size: 114 240 229 1120 4976
Fragments arranged by size:
4976 1120 240 229 114
ApaLI G'TGCA_C
Cuts at: 3148 3323 4736 5892 3148
Size: 175 1413 1156 3935
Fragments arranged by size:
3935 1413 1156 175
ApoI r'AATT_y
Cuts at: 1493 3988 4214 4366 6475 1493
Size: 2495 226 152 2109 1697
Fragments arranged by size:
2495 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5233 160
Size: 872 4201 1606
Fragments arranged by size:
4201 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6679
AvaI C'yCGr_G
Cuts at: 1870 1943 2114 2280 2395 2585 2735 3575 4004
Size: 73 171 166 115 190 150 840 429
Cuts at: 4004 1870
Size: 4545
Fragments arranged by size:
4545 840 429 190 171 166 150 115
73
BamHI G'GATC_C
Cuts at: 4009 4376 4009
Size: 367 6312
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2270 3509 3638 3797 4000
Size: 325 217 627 483 1239 129 159 203
Cuts at: 4000 5455 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1239 627 483 325 217 203
159 129
BanII G_rGCy'C
Cuts at: 1391 2114 2228 2394 2468 2697 3302 3817 3879
Size: 723 114 166 74 229 605 515 62
Cuts at: 3879 3998 1391
Size: 119 4072
Fragments arranged by size:
4072 723 605 515 229 166 119 114
74 62
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2311 3700 928
Size: 971 412 1389 3907
Fragments arranged by size:
3907 1389 971 412
BbvCI CC'TCA_GC
Cuts at: 2060 3371 3644 2060
Size: 1311 273 5095
Fragments arranged by size:
5095 1311 273
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3745 3779 4870 4904 1903
Size: 34 1808 34 1091 34 3678
Fragments arranged by size:
3678 1808 1091 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4571 5781 4571
Size: 1210 5469
BclI T'GATC_A
Cuts at: 1548 3264 1548
Size: 1716 4963
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5290 136
Size: 107 122 71 631 605 3618 1525
Fragments arranged by size:
3618 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2814 2844 6674 2814
Size: 30 3830 2819
Fragments arranged by size:
3830 2819 30
BlpI GC'TnA_GC
Cuts at: 2484 4096 2484
Size: 1612 5067
Bme1580I G_kGCm'C
Cuts at: 1790 2114 2228 2468 2697 3152 3327 3817 4740
Size: 324 114 240 229 455 175 490 923
Cuts at: 4740 5896 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 923 490 455 324 240 229
175 114
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6679
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2528 4410 5368
Size: 416 53 83 399 24 1111 1882 958
Cuts at: 5368 442
Size: 1753
Fragments arranged by size:
1882 1753 1111 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6679
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2231 2234 2258 2315 2589 2847 2971 3217
Size: 992 3 24 57 274 258 124 246
Cuts at: 3217 3371 3452 5339 1239
Size: 154 81 1887 2579
Fragments arranged by size:
2579 1887 992 274 258 246 154 124
81 57 24 3
Bpu10I CC'TnA_GC
Cuts at: 2060 2169 3371 3644 2060
Size: 109 1202 273 5095
Fragments arranged by size:
5095 1202 273 109
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2465 2877 3666 4804 5669 5967 2465
Size: 412 789 1138 865 298 3177
Fragments arranged by size:
3177 1138 865 789 412 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2155 3684 5342 882
Size: 617 656 1529 1658 2219
Fragments arranged by size:
2219 1658 1529 656 617
BsaAI yAC'GTr
Cuts at: 493 1204 2779 4470 493
Size: 711 1575 1691 2702
Fragments arranged by size:
2702 1691 1575 711
BsaBI GATnn'nnATC
Cuts at: 6673 6673
Size: 6679
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2796 3102 4486 4868
Size: 53 83 186 936 1263 306 1384 382
Cuts at: 4868 5503 275
Size: 635 1451
Fragments arranged by size:
1451 1384 1263 936 635 382 306 186
83 53
BsaWI w'CCGG_w
Cuts at: 5112 5510 5784 5931 6342 5112
Size: 398 274 147 411 5449
Fragments arranged by size:
5449 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3022 3052 3369 3399 3498
Size: 30 165 30 1619 30 317 30 99
Cuts at: 3498 3528 1178
Size: 30 4329
Fragments arranged by size:
4329 1619 317 165 99 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2501 3027 3593 3895 1186
Size: 1315 526 566 302 3970
Fragments arranged by size:
3970 1315 566 526 302
BseYI C'CCAG_C
Cuts at: 3179 3358 3781 3863 5882 6378 3179
Size: 179 423 82 2019 496 3480
Fragments arranged by size:
3480 2019 496 423 179 82
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2336 3449 3634 3813 1163
Size: 573 600 1113 185 179 4029
Fragments arranged by size:
4029 1113 600 573 185 179
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2597 2691 4129 4890 5918 1540
Size: 275 186 596 94 1438 761 1028 2301
Fragments arranged by size:
2301 1438 1028 761 596 275 186 94
BsiHKAI G_wGCw'C
Cuts at: 3152 3302 3327 3944 3953 4740 4825 5896 3152
Size: 150 25 617 9 787 85 1071 3935
Fragments arranged by size:
3935 1071 787 617 150 85 25 9
BsmI GAATG_Cn'
Cuts at: 4190 4283 4190
Size: 93 6586
BsmBI CGTCTCn'nnnn_
Cuts at: 3457 3457
Size: 6679
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2114 2228 2394 2468 2697 3152 3302
Size: 399 324 114 166 74 229 455 150
Cuts at: 3302 3327 3817 3879 3944 3953 3998 4740 4825
Size: 25 490 62 65 9 45 742 85
Cuts at: 4825 5896 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 490 455 399 324 229
166 150 114 85 74 65 62 45
25 9
BspEI T'CCGG_A
Cuts at: 6342 6342
Size: 6679
BspHI T'CATG_A
Cuts at: 4568 6298 4568
Size: 1730 4949
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3238 3421 4034 844
Size: 2394 183 613 3489
Fragments arranged by size:
3489 2394 613 183
BsrBI CCG'CTC
Cuts at: 1394 1823 4566 1394
Size: 429 2743 3507
Fragments arranged by size:
3507 2743 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3277 5174 5348 65
Size: 1183 2029 1897 174 1396
Fragments arranged by size:
2029 1897 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2642 3635 5323 5510 1700
Size: 110 832 993 1688 187 2869
Fragments arranged by size:
2869 1688 993 832 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6679
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6679
BssSI C'ACGA_G
Cuts at: 4739 5751 4739
Size: 1012 5667
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3957 794
Size: 3163 3516
BstBI TT'CG_AA
Cuts at: 5483 5483
Size: 6679
BstEII G'GTnAC_C
Cuts at: 1184 2377 3581 1184
Size: 1193 1204 4282
Fragments arranged by size:
4282 1204 1193
BstXI CCAn_nnnn'nTGG
Cuts at: 2935 3112 3973 2935
Size: 177 861 5641
Fragments arranged by size:
5641 861 177
BstYI r'GATC_y
Cuts at: 1972 2439 2740 2814 2844 4009 4376 4761 4778
Size: 467 301 74 30 1165 367 385 17
Cuts at: 4778 6230 6316 6328 6674 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1165 467 385 367 346 301
86 74 30 17 12
Bsu36I CC'TnA_GG
Cuts at: 2666 3501 2666
Size: 835 5844
BtgI C'CryG_G
Cuts at: 513 1065 2004 2011 3513 3879 513
Size: 552 939 7 1502 366 3313
Fragments arranged by size:
3313 1502 939 552 366 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5940
Fragments arranged by size:
5940 683 56
BtsI GCAGTG_nn'
Cuts at: 2320 2348 3229 3325 3822 4195 4989 5009 2320
Size: 28 881 96 497 373 794 20 3990
Fragments arranged by size:
3990 881 794 497 373 96 28 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6679
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2056 2091 2337 2372 529
Size: 35 1492 35 246 35 4836
Fragments arranged by size:
4836 1492 246 35 35 35
DraI TTT'AAA
Cuts at: 3983 3983
Size: 6679
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3212 3692 5686 809
Size: 507 1896 480 1994 1802
Fragments arranged by size:
1994 1896 1802 507 480
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2594 2640
Size: 54 1425 50 275 18 216 548 46
Cuts at: 2640 2644 2688 2961 3537 4126 5015 6644 8
Size: 4 44 273 576 589 889 1629 43
Fragments arranged by size:
1629 1425 889 589 576 548 275 273
216 54 50 46 44 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2594 2688 4126 1537
Size: 275 782 94 1438 4090
Fragments arranged by size:
4090 1438 782 275 94
EarI CTCTTCn'nnn_
Cuts at: 1353 2210 4609 1353
Size: 857 2399 3423
Fragments arranged by size:
3423 2399 857
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5266 5640 5786 387
Size: 1064 138 3677 374 146 1280
Fragments arranged by size:
3677 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3300 3300
Size: 6679
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2231 2234 2258 2315 2589 2847
Size: 393 452 147 3 24 57 274 258
Cuts at: 2847 2971 3217 3371 3452 3577 4742 5339 6126
Size: 124 246 154 81 125 1165 597 787
Cuts at: 6126 6394 1239
Size: 268 1524
Fragments arranged by size:
1524 1165 787 597 452 393 274 268
258 246 154 147 125 124 81 57
24 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2906 3504 2906
Size: 598 6081
EcoO109I rG'GnC_Cy
Cuts at: 2111 2173 2335 2464 2465 2671 2694 2768 3175
Size: 62 162 129 1 206 23 74 407
Cuts at: 3175 3497 3813 3814 2111
Size: 322 316 1 4976
Fragments arranged by size:
4976 407 322 316 206 162 129 74
62 23 1 1
EcoRI G'AATT_C
Cuts at: 3988 4366 3988
Size: 378 6301
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3232 3232
Size: 6679
FseI GG_CCGG'CC
Cuts at: 2646 2646
Size: 6679
FspI TGC'GCA
Cuts at: 2731 3138 3221 5185 2731
Size: 407 83 1964 4225
Fragments arranged by size:
4225 1964 407 83
HaeII r_GCGC'y
Cuts at: 1678 5826 1678
Size: 4148 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2405 2437 3022 3054 3332
Size: 32 135 32 833 32 585 32 278
Cuts at: 3332 3364 3664 3696 5320 5352 5394 5426 1373
Size: 32 300 32 1624 32 42 32 2626
Fragments arranged by size:
2626 1624 833 585 300 278 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2404 4023 4269 669
Size: 988 281 466 1619 246 3079
Fragments arranged by size:
3079 1619 988 466 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6679
HpaI GTT'AAC
Cuts at: 4269 4269
Size: 6679
KpnI G_GTAC'C
Cuts at: 4004 4004
Size: 6679
MfeI C'AATT_G
Cuts at: 4278 4278
Size: 6679
MluI A'CGCG_T
Cuts at: 3854 6555 3854
Size: 2701 3978
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2539 3420 5793 5977 1305
Size: 1234 881 2373 184 2007
Fragments arranged by size:
2373 2007 1234 881 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2048 2963 10
Size: 54 1425 343 216 915 3726
Fragments arranged by size:
3726 1425 915 343 216 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3248 4637 4996 5155 518
Size: 648 2082 1389 359 159 2042
Fragments arranged by size:
2082 2042 1389 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2013 2052 2236 2456 2959 2991 3183 3237
Size: 407 39 184 220 503 32 192 54
Cuts at: 3237 3313 3358 3480 3648 4095 4772 5920 6165
Size: 76 45 122 168 447 677 1148 245
Cuts at: 6165 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 503 447 407 245 220
192 184 168 122 76 54 45 39
32
NaeI GCC'GGC
Cuts at: 2644 2644
Size: 6679
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5188
Fragments arranged by size:
5188 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6679
NgoMIV G'CCGG_C
Cuts at: 2642 2642
Size: 6679
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6679
NotI GC'GGCC_GC
Cuts at: 2594 4126 2594
Size: 1532 5147
NspI r_CATG'y
Cuts at: 1132 1229 2554 2812 3247 4037 5582 1132
Size: 97 1325 258 435 790 1545 2229
Fragments arranged by size:
2229 1545 1325 790 435 258 97
PasI CC'CwG_GG
Cuts at: 1348 2338 2530 1348
Size: 990 192 5497
Fragments arranged by size:
5497 990 192
PciI A'CATG_T
Cuts at: 5578 5578
Size: 6679
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2338 2572 1108
Size: 1230 234 5215
Fragments arranged by size:
5215 1230 234
PfoI T'CCnGG_A
Cuts at: 2208 2208
Size: 6679
PmeI GTTT'AAAC
Cuts at: 3983 3983
Size: 6679
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4748 4780 6261 6293 4748
Size: 32 1481 32 5134
Fragments arranged by size:
5134 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2671 2768 3175 2671
Size: 97 407 6175
Fragments arranged by size:
6175 407 97
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6679
PsiI TTA'TAA
Cuts at: 4249 4249
Size: 6679
PspOMI G'GGCC_C
Cuts at: 2110 2224 2464 2693 3813 2110
Size: 114 240 229 1120 4976
Fragments arranged by size:
4976 1120 240 229 114
PspXI vC'TCGA_Gb
Cuts at: 2585 2585
Size: 6679
PstI C_TGCA'G
Cuts at: 830 2057 2766 3009 4031 830
Size: 1227 709 243 1022 3478
Fragments arranged by size:
3478 1227 1022 709 243
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6679
PvuII CAG'CTG
Cuts at: 2052 2236 2456 2991 3183 3237 3358 3480 3648
Size: 184 220 535 192 54 121 122 168
Cuts at: 3648 2052
Size: 5083
Fragments arranged by size:
5083 535 220 192 184 168 122 121
54
SacI G_AGCT'C
Cuts at: 3302 3302
Size: 6679
SacII CC_GC'GG
Cuts at: 2014 2014
Size: 6679
SalI G'TCGA_C
Cuts at: 1936 4021 1936
Size: 2085 4594
SanDI GG'GwC_CC
Cuts at: 2768 3175 2768
Size: 407 6272
SbfI CC_TGCA'GG
Cuts at: 2766 4031 2766
Size: 1265 5414
ScaI AGT'ACT
Cuts at: 4927 6639 4927
Size: 1712 4967
SfcI C'TryA_G
Cuts at: 826 1045 2053 2762 3005 4027 5162 5843 6034
Size: 219 1008 709 243 1022 1135 681 191
Cuts at: 6034 826
Size: 1471
Fragments arranged by size:
1471 1135 1022 1008 709 681 243 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6679
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6679
SmaI CCC'GGG
Cuts at: 1872 2397 2737 4006 1872
Size: 525 340 1269 4545
Fragments arranged by size:
4545 1269 525 340
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2444 2585 2892 3681 4783 5684
Size: 197 926 501 141 307 789 1102 901
Cuts at: 5684 5946 820
Size: 262 1553
Fragments arranged by size:
1553 1102 926 901 789 501 307 262
197 141
SnaBI TAC'GTA
Cuts at: 493 4470 493
Size: 3977 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6679
SphI G_CATG'C
Cuts at: 1132 2554 3247 4037 1132
Size: 1422 693 790 3774
Fragments arranged by size:
3774 1422 790 693
SspI AAT'ATT
Cuts at: 5 52 4603 5
Size: 47 4551 2081
Fragments arranged by size:
4551 2081 47
StuI AGG'CCT
Cuts at: 1517 2898 3392 3762 1517
Size: 1381 494 370 4434
Fragments arranged by size:
4434 1381 494 370
StyI C'CwwG_G
Cuts at: 513 1065 2004 2610 3042 3540 4118 513
Size: 552 939 606 432 498 578 3074
Fragments arranged by size:
3074 939 606 578 552 498 432
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3959 4706 4723 4876 5061 5506 325
Size: 1255 2379 747 17 153 185 445 1498
Fragments arranged by size:
2379 1498 1255 747 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4925 6637 96
Size: 274 80 33 51 1443 2948 1712 138
Fragments arranged by size:
2948 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3369 3401 3498 3530 3842 3874 6639 6671 3369
Size: 32 97 32 312 32 2765 32 3377
Fragments arranged by size:
3377 2765 312 97 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3676 1316
Size: 2360 4319
XbaI T'CTAG_A
Cuts at: 4015 4015
Size: 6679
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2569 3970 2569
Size: 1401 5278
XhoI C'TCGA_G
Cuts at: 1943 2585 1943
Size: 642 6037
XmaI C'CCGG_G
Cuts at: 1870 2395 2735 4004 1870
Size: 525 340 1269 4545
Fragments arranged by size:
4545 1269 525 340
XmnI GAAnn'nnTTC
Cuts at: 4808 4808
Size: 6679
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4487 276
Size: 53 83 186 936 2953 2468
Fragments arranged by size:
2953 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstZ17I DraIII EcoRV FspAI KasI NarI NruI
NsiI PacI PmlI PsrI RsrII SapI SexAI SfiI
SfoI SrfI SwaI