Restriction map for
pFN21AE1374
(Circular) (Six-base) MAPSORT of: pFN21AE1374.seq Check: 9895 from: 1 to: 5989
pFN21AE1374 5989 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3140 3799 278
Size: 53 83 186 936 1604 659 2468
Fragments arranged by size:
2468 1604 936 659 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3332 1937
Size: 1395 4594
Acc65I G'GTAC_C
Cuts at: 3310 3310
Size: 5989
AclI AA'CG_TT
Cuts at: 1563 4116 4489 1563
Size: 2553 373 3063
Fragments arranged by size:
3063 2553 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4052 5436 5704 1632
Size: 2420 1384 268 1917
Fragments arranged by size:
2420 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5792
AflIII A'CryG_T
Cuts at: 1203 4888 5865 1203
Size: 3685 977 1327
Fragments arranged by size:
3685 1327 977
AgeI A'CCGG_T
Cuts at: 4820 4820
Size: 5989
AhdI GACnn_n'nnGTC
Cuts at: 4718 4718
Size: 5989
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5989
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5989
AlwNI CAG_nnn'CTG
Cuts at: 3732 5304 3732
Size: 1572 4417
ApaLI G'TGCA_C
Cuts at: 3080 4046 5202 3080
Size: 966 1156 3867
Fragments arranged by size:
3867 1156 966
ApoI r'AATT_y
Cuts at: 1493 3298 3524 3676 5785 1493
Size: 1805 226 152 2109 1697
Fragments arranged by size:
2109 1805 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4543 160
Size: 872 3511 1606
Fragments arranged by size:
3511 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5989
AvaI C'yCGr_G
Cuts at: 1870 1943 2201 2261 3314 1870
Size: 73 258 60 1053 4545
Fragments arranged by size:
4545 1053 258 73 60
BamHI G'GATC_C
Cuts at: 3319 3686 3319
Size: 367 5622
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2305 2766 3310 4765 618
Size: 325 217 627 518 461 544 1455 1842
Fragments arranged by size:
1842 1455 627 544 518 461 325 217
BanII G_rGCy'C
Cuts at: 1391 2606 2697 2861 2888 3104 3308 1391
Size: 1215 91 164 27 216 204 4072
Fragments arranged by size:
4072 1215 216 204 164 91 27
BbeI G_GCGC'C
Cuts at: 2309 2309
Size: 5989
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2877 2990 928
Size: 971 978 113 3927
Fragments arranged by size:
3927 978 971 113
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2150 2184 4180 4214 1903
Size: 34 213 34 1996 34 3678
Fragments arranged by size:
3678 1996 213 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3881 5091 3881
Size: 1210 4779
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5989
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2892 4600 136
Size: 107 122 71 631 605 1220 1708 1525
Fragments arranged by size:
1708 1525 1220 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3226 5984 3226
Size: 2758 3231
BlpI GC'TnA_GC
Cuts at: 2285 3406 2285
Size: 1121 4868
Bme1580I G_kGCm'C
Cuts at: 1790 2769 3084 4050 5206 1790
Size: 979 315 966 1156 2573
Fragments arranged by size:
2573 1156 979 966 315
BmgBI CAC'GTC
Cuts at: 1318 2074 2875 1318
Size: 756 801 4432
Fragments arranged by size:
4432 801 756
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3720 4678 442
Size: 416 53 83 399 24 2303 958 1753
Fragments arranged by size:
2303 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5989
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2453 2815 3028 4649 1239
Size: 1214 362 213 1621 2579
Fragments arranged by size:
2579 1621 1214 362 213
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4114 4979 5277 4114
Size: 865 298 4826
Fragments arranged by size:
4826 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4652 882
Size: 617 3153 2219
Fragments arranged by size:
3153 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3780 493
Size: 711 2576 2702
Fragments arranged by size:
2702 2576 711
BsaBI GATnn'nnATC
Cuts at: 5983 5983
Size: 5989
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2222 2306 3137 3796
Size: 53 83 186 936 689 84 831 659
Cuts at: 3796 4178 4813 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 831 689 659 635 382 186
84 83 53
BsaWI w'CCGG_w
Cuts at: 4422 4820 5094 5241 5652 4422
Size: 398 274 147 411 4759
Fragments arranged by size:
4759 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3098 3128 1178
Size: 30 165 30 1695 30 4039
Fragments arranged by size:
4039 1695 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2177 2818 1186
Size: 991 641 4357
Fragments arranged by size:
4357 991 641
BseYI C'CCAG_C
Cuts at: 2188 2788 2819 2912 3054 3250 5192 5688 2188
Size: 600 31 93 142 196 1942 496 2489
Fragments arranged by size:
2489 1942 600 496 196 142 93 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2516 1163
Size: 573 780 4636
Fragments arranged by size:
4636 780 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2646 3439 4200 5228 1540
Size: 275 186 645 793 761 1028 2301
Fragments arranged by size:
2301 1028 793 761 645 275 186
BsiHKAI G_wGCw'C
Cuts at: 2322 2441 2697 3084 4050 4135 5206 2322
Size: 119 256 387 966 85 1071 3105
Fragments arranged by size:
3105 1071 966 387 256 119 85
BsmI GAATG_Cn'
Cuts at: 3500 3593 3500
Size: 93 5896
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2322 2441 2606 2697 2769 2861 2888
Size: 399 532 119 165 91 72 92 27
Cuts at: 2888 3084 3104 3308 4050 4135 5206 1391
Size: 196 20 204 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 532 399 204 196 165
119 92 91 85 72 27 20
BspEI T'CCGG_A
Cuts at: 5652 5652
Size: 5989
BspHI T'CATG_A
Cuts at: 3878 5608 3878
Size: 1730 4259
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2112 3344 844
Size: 1268 1232 3489
Fragments arranged by size:
3489 1268 1232
BsrBI CCG'CTC
Cuts at: 1394 1823 2020 3119 3220 3876 1394
Size: 429 197 1099 101 656 3507
Fragments arranged by size:
3507 1099 656 429 197 101
BsrDI GCAATG_nn'
Cuts at: 65 1248 4484 4658 65
Size: 1183 3236 174 1396
Fragments arranged by size:
3236 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2294 3063 4633 4820 1700
Size: 110 484 769 1570 187 2869
Fragments arranged by size:
2869 1570 769 484 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5989
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5989
BssSI C'ACGA_G
Cuts at: 2138 2881 2923 4049 5061 2138
Size: 743 42 1126 1012 3066
Fragments arranged by size:
3066 1126 1012 743 42
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5989
BstBI TT'CG_AA
Cuts at: 4793 4793
Size: 5989
BstEII G'GTnAC_C
Cuts at: 1184 2477 1184
Size: 1293 4696
BstXI CCAn_nnnn'nTGG
Cuts at: 2635 3266 2635
Size: 631 5358
BstYI r'GATC_y
Cuts at: 1972 3226 3319 3686 4071 4088 5540 5626 5638
Size: 1254 93 367 385 17 1452 86 12
Cuts at: 5638 5984 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1254 385 367 346 93 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2718 513
Size: 552 1653 3784
Fragments arranged by size:
3784 1653 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3058 487
Size: 683 56 1832 3418
Fragments arranged by size:
3418 1832 683 56
BtsI GCAGTG_nn'
Cuts at: 2477 3505 4299 4319 2477
Size: 1028 794 20 4147
Fragments arranged by size:
4147 1028 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5989
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5954
DraI TTT'AAA
Cuts at: 3293 3293
Size: 5989
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4996 809
Size: 507 3680 1802
Fragments arranged by size:
3680 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2235 2517 3180
Size: 54 1425 50 275 18 405 282 663
Cuts at: 3180 3436 4325 5954 8
Size: 256 889 1629 43
Fragments arranged by size:
1629 1425 889 663 405 282 275 256
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3436 1537
Size: 275 1624 4090
Fragments arranged by size:
4090 1624 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3919 1353
Size: 2566 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2390 4576 4950 5096 387
Size: 1064 138 801 2186 374 146 1280
Fragments arranged by size:
2186 1280 1064 801 374 146 138
EcoICRI GAG'CTC
Cuts at: 2695 2695
Size: 5989
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2453 2815 3028 4052 4649 5436 5704
Size: 393 821 362 213 1024 597 787 268
Cuts at: 5704 1239
Size: 1524
Fragments arranged by size:
1524 1024 821 787 597 393 362 268
213
EcoNI CCTnn'n_nnAGG
Cuts at: 2030 2417 2459 2030
Size: 387 42 5560
Fragments arranged by size:
5560 387 42
EcoO109I rG'GnC_Cy
Cuts at: 2059 2735 3214 2059
Size: 676 479 4834
Fragments arranged by size:
4834 676 479
EcoRI G'AATT_C
Cuts at: 3298 3676 3298
Size: 378 5611
FspI TGC'GCA
Cuts at: 2246 2469 4495 2246
Size: 223 2026 3740
Fragments arranged by size:
3740 2026 223
HaeII r_GCGC'y
Cuts at: 1678 2309 2455 5136 1678
Size: 631 146 2681 2531
Fragments arranged by size:
2681 2531 631 146
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3218 3250 4630 4662 4704
Size: 32 135 32 1646 32 1380 32 42
Cuts at: 4704 4736 1373
Size: 32 2626
Fragments arranged by size:
2626 1646 1380 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3333 3579 669
Size: 988 281 1395 246 3079
Fragments arranged by size:
3079 1395 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5989
HpaI GTT'AAC
Cuts at: 3579 3579
Size: 5989
KasI G'GCGC_C
Cuts at: 2305 2305
Size: 5989
KpnI G_GTAC'C
Cuts at: 3314 3314
Size: 5989
MfeI C'AATT_G
Cuts at: 3588 3588
Size: 5989
MluI A'CGCG_T
Cuts at: 5865 5865
Size: 5989
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2349 5103 5287 1305
Size: 1044 2754 184 2007
Fragments arranged by size:
2754 2007 1044 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2237 10
Size: 54 1425 343 405 3762
Fragments arranged by size:
3762 1425 405 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2513 2702 3077 3947 4306 4465 518
Size: 648 1347 189 375 870 359 159 2042
Fragments arranged by size:
2042 1347 870 648 375 359 189 159
MspA1I CmG'CkG
Cuts at: 1606 2431 3087 3405 4082 5230 5475 1606
Size: 825 656 318 677 1148 245 2120
Fragments arranged by size:
2120 1148 825 677 656 318 245
NarI GG'CG_CC
Cuts at: 2306 2306
Size: 5989
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5437
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5989
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5989
NotI GC'GGCC_GC
Cuts at: 3436 3436
Size: 5989
NspI r_CATG'y
Cuts at: 1132 1229 3347 4892 1132
Size: 97 2118 1545 2229
Fragments arranged by size:
2229 2118 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2749 1348
Size: 1401 4588
PciI A'CATG_T
Cuts at: 4888 4888
Size: 5989
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5989
PfoI T'CCnGG_A
Cuts at: 2652 2708 2652
Size: 56 5933
PmeI GTTT'AAAC
Cuts at: 3293 3293
Size: 5989
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4058 4090 5571 5603 4058
Size: 32 1481 32 4444
Fragments arranged by size:
4444 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2735 3214 2735
Size: 479 5510
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5989
PsiI TTA'TAA
Cuts at: 3559 3559
Size: 5989
PstI C_TGCA'G
Cuts at: 830 2109 2763 3341 830
Size: 1279 654 578 3478
Fragments arranged by size:
3478 1279 654 578
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5989
SacI G_AGCT'C
Cuts at: 2697 2697
Size: 5989
SalI G'TCGA_C
Cuts at: 1936 3331 1936
Size: 1395 4594
SbfI CC_TGCA'GG
Cuts at: 2109 3341 2109
Size: 1232 4757
ScaI AGT'ACT
Cuts at: 2126 4237 5949 2126
Size: 2111 1712 2166
Fragments arranged by size:
2166 2111 1712
SexAI A'CCwGG_T
Cuts at: 2093 2397 2093
Size: 304 5685
SfcI C'TryA_G
Cuts at: 826 1045 2105 2759 3337 4472 5153 5344 826
Size: 219 1060 654 578 1135 681 191 1471
Fragments arranged by size:
1471 1135 1060 681 654 578 219 191
SfoI GGC'GCC
Cuts at: 2307 2307
Size: 5989
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5989
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5989
SmaI CCC'GGG
Cuts at: 1872 3316 1872
Size: 1444 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4093 4994 5256 820
Size: 197 926 2150 901 262 1553
Fragments arranged by size:
2150 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3780 493
Size: 3287 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5989
SphI G_CATG'C
Cuts at: 1132 3347 1132
Size: 2215 3774
SspI AAT'ATT
Cuts at: 5 52 3913 5
Size: 47 3861 2081
Fragments arranged by size:
3861 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5989
StyI C'CwwG_G
Cuts at: 513 1065 3428 513
Size: 552 2363 3074
Fragments arranged by size:
3074 2363 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2045 2660 3068 4016 4033 4186 4371
Size: 1255 465 615 408 948 17 153 185
Cuts at: 4371 4816 325
Size: 445 1498
Fragments arranged by size:
1498 1255 948 615 465 445 408 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2124 4235 5947
Size: 274 80 33 51 1443 147 2111 1712
Cuts at: 5947 96
Size: 138
Fragments arranged by size:
2111 1712 1443 274 147 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2369 2401 3098 3130 5949 5981 2369
Size: 32 697 32 2819 32 2377
Fragments arranged by size:
2819 2377 697 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2399 1316
Size: 1083 4906
XbaI T'CTAG_A
Cuts at: 3325 3325
Size: 5989
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2746 2746
Size: 5989
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5989
XmaI C'CCGG_G
Cuts at: 1870 3314 1870
Size: 1444 4545
XmnI GAAnn'nnTTC
Cuts at: 4118 4118
Size: 5989
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3138 3797 276
Size: 53 83 186 936 1604 659 2468
Fragments arranged by size:
2468 1604 936 659 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacI SalI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SapI SfiI SrfI SwaI