Restriction map for
pFN21AE1373
(Circular) (Six-base) MAPSORT of: pFN21AE1373.seq Check: 3815 from: 1 to: 6022
pFN21AE1373 6022 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2568 2568
Size: 6022
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3832 278
Size: 53 83 186 936 2296 2468
Fragments arranged by size:
2468 2296 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3365 1937
Size: 1428 4594
Acc65I G'GTAC_C
Cuts at: 3343 3343
Size: 6022
AclI AA'CG_TT
Cuts at: 1563 4149 4522 1563
Size: 2586 373 3063
Fragments arranged by size:
3063 2586 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4085 5469 5737 1632
Size: 2453 1384 268 1917
Fragments arranged by size:
2453 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5825
AflIII A'CryG_T
Cuts at: 1203 4921 5898 1203
Size: 3718 977 1327
Fragments arranged by size:
3718 1327 977
AgeI A'CCGG_T
Cuts at: 4853 4853
Size: 6022
AhdI GACnn_n'nnGTC
Cuts at: 4751 4751
Size: 6022
AleI CACnn'nnGTG
Cuts at: 1166 3237 1166
Size: 2071 3951
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6022
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2501 2533 2501
Size: 32 5990
AlwNI CAG_nnn'CTG
Cuts at: 2114 3765 5337 2114
Size: 1651 1572 2799
Fragments arranged by size:
2799 1651 1572
ApaI G_GGCC'C
Cuts at: 2079 2561 2861 3005 2079
Size: 482 300 144 5096
Fragments arranged by size:
5096 482 300 144
ApaLI G'TGCA_C
Cuts at: 4079 5235 4079
Size: 1156 4866
ApoI r'AATT_y
Cuts at: 1493 3146 3331 3557 3709 5818 1493
Size: 1653 185 226 152 2109 1697
Fragments arranged by size:
2109 1697 1653 226 185 152
AseI AT'TA_AT
Cuts at: 160 1032 4576 160
Size: 872 3544 1606
Fragments arranged by size:
3544 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6022
AvaI C'yCGr_G
Cuts at: 1870 1943 2036 2538 3347 1870
Size: 73 93 502 809 4545
Fragments arranged by size:
4545 809 502 93 73
BamHI G'GATC_C
Cuts at: 2220 3352 3719 2220
Size: 1132 367 4523
Fragments arranged by size:
4523 1132 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3343 4798 618
Size: 325 217 627 1556 1455 1842
Fragments arranged by size:
1842 1556 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2079 2561 2570 2655 2835 2861 3005 3255
Size: 688 482 9 85 180 26 144 250
Cuts at: 3255 3341 1391
Size: 86 4072
Fragments arranged by size:
4072 688 482 250 180 144 86 85
26 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5051
BbvCI CC'TCA_GC
Cuts at: 3281 3281
Size: 6022
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4213 4247 1903
Size: 34 2276 34 3678
Fragments arranged by size:
3678 2276 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3914 5124 3914
Size: 1210 4812
BclI T'GATC_A
Cuts at: 1548 2118 2955 1548
Size: 570 837 4615
Fragments arranged by size:
4615 837 570
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4633 136
Size: 107 122 71 631 605 2961 1525
Fragments arranged by size:
2961 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6017 6017
Size: 6022
BlpI GC'TnA_GC
Cuts at: 2042 2103 3439 2042
Size: 61 1336 4625
Fragments arranged by size:
4625 1336 61
Bme1580I G_kGCm'C
Cuts at: 1790 2079 2561 2861 3005 4083 5239 1790
Size: 289 482 300 144 1078 1156 2573
Fragments arranged by size:
2573 1156 1078 482 300 289 144
BmgBI CAC'GTC
Cuts at: 1318 2792 1318
Size: 1474 4548
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2631 3231 3753
Size: 416 53 83 399 24 1214 600 522
Cuts at: 3753 4711 442
Size: 958 1753
Fragments arranged by size:
1753 1214 958 600 522 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6022
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2128 2128
Size: 6022
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2183 2366 2786 3269 4682 1239
Size: 944 183 420 483 1413 2579
Fragments arranged by size:
2579 1413 944 483 420 183
Bpu10I CC'TnA_GC
Cuts at: 3281 3281
Size: 6022
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4147 5012 5310 4147
Size: 865 298 4859
Fragments arranged by size:
4859 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2634 3265 3269 4685 882
Size: 617 1135 631 4 1416 2219
Fragments arranged by size:
2219 1416 1135 631 617 4
BsaAI yAC'GTr
Cuts at: 493 1204 2065 3813 493
Size: 711 861 1748 2702
Fragments arranged by size:
2702 1748 861 711
BsaBI GATnn'nnATC
Cuts at: 6016 6016
Size: 6022
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3829 4211 4846 275
Size: 53 83 186 936 2296 382 635 1451
Fragments arranged by size:
2296 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2897 4455 4853 5127 5274 5685 2897
Size: 1558 398 274 147 411 3234
Fragments arranged by size:
3234 1558 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2409 2439 2879 2897 2900
Size: 30 165 30 1006 30 440 18 3
Cuts at: 2900 2909 2927 2930 3012 3042 1178
Size: 9 18 3 82 30 4158
Fragments arranged by size:
4158 1006 440 165 82 30 30 30
30 18 18 9 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2905 1186
Size: 1719 4303
BseYI C'CCAG_C
Cuts at: 2396 2828 2933 5225 5721 2396
Size: 432 105 2292 496 2697
Fragments arranged by size:
2697 2292 496 432 105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2087 2714 1163
Size: 573 351 627 4471
Fragments arranged by size:
4471 627 573 351
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2999 3472 4233 5261 1540
Size: 275 186 998 473 761 1028 2301
Fragments arranged by size:
2301 1028 998 761 473 275 186
BsiHKAI G_wGCw'C
Cuts at: 3255 4083 4168 5239 3255
Size: 828 85 1071 4038
Fragments arranged by size:
4038 1071 828 85
BsmI GAATG_Cn'
Cuts at: 3533 3626 3533
Size: 93 5929
BsmBI CGTCTCn'nnnn_
Cuts at: 2159 2159
Size: 6022
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2561 2570 2655 2835 2861 3005
Size: 399 289 482 9 85 180 26 144
Cuts at: 3005 3255 3341 4083 4168 5239 1391
Size: 250 86 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 482 399 289 250 180
144 86 85 85 26 9
BspEI T'CCGG_A
Cuts at: 5685 5685
Size: 6022
BspHI T'CATG_A
Cuts at: 3911 5641 3911
Size: 1730 4292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2568 3231 3377 844
Size: 1724 663 146 3489
Fragments arranged by size:
3489 1724 663 146
BsrBI CCG'CTC
Cuts at: 1394 1823 2871 3909 1394
Size: 429 1048 1038 3507
Fragments arranged by size:
3507 1048 1038 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4517 4691 65
Size: 1183 3269 174 1396
Fragments arranged by size:
3269 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2753 2814 4666 4853 1700
Size: 110 943 61 1852 187 2869
Fragments arranged by size:
2869 1852 943 187 110 61
BsrGI T'GTAC_A
Cuts at: 96 2775 96
Size: 2679 3343
BssHII G'CGCG_C
Cuts at: 1925 2611 1925
Size: 686 5336
BssSI C'ACGA_G
Cuts at: 3254 4082 5094 3254
Size: 828 1012 4182
Fragments arranged by size:
4182 1012 828
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6022
BstBI TT'CG_AA
Cuts at: 4826 4826
Size: 6022
BstEII G'GTnAC_C
Cuts at: 1184 2407 1184
Size: 1223 4799
BstXI CCAn_nnnn'nTGG
Cuts at: 2404 2528 2810 2404
Size: 124 282 5616
Fragments arranged by size:
5616 282 124
BstYI r'GATC_y
Cuts at: 1972 2220 2967 3352 3719 4104 4121 5573 5659
Size: 248 747 385 367 385 17 1452 86
Cuts at: 5659 5671 6017 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 747 385 385 367 346 248
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2144 2413 3016 2144
Size: 269 603 5150
Fragments arranged by size:
5150 603 269
BtgI C'CryG_G
Cuts at: 513 1065 2527 2553 513
Size: 552 1462 26 3982
Fragments arranged by size:
3982 1462 552 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5283
Fragments arranged by size:
5283 683 56
BtsI GCAGTG_nn'
Cuts at: 2341 3538 4332 4352 2341
Size: 1197 794 20 4011
Fragments arranged by size:
4011 1197 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6022
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5987
DraI TTT'AAA
Cuts at: 3326 3326
Size: 6022
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5029 809
Size: 507 3713 1802
Fragments arranged by size:
3713 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2812 2996 3469
Size: 54 1425 50 275 18 982 184 473
Cuts at: 3469 4358 5987 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 982 889 473 275 184 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2996 3469 1537
Size: 275 1184 473 4090
Fragments arranged by size:
4090 1184 473 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3952 1353
Size: 2599 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4609 4983 5129 387
Size: 1064 138 3020 374 146 1280
Fragments arranged by size:
3020 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3253 3253
Size: 6022
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 2366 2786 3269 4085 4682 5469
Size: 393 551 183 420 483 816 597 787
Cuts at: 5469 5737 1239
Size: 268 1524
Fragments arranged by size:
1524 816 787 597 551 483 420 393
268 183
EcoO109I rG'GnC_Cy
Cuts at: 2058 2075 2076 2558 2702 2858 3002 3183 2058
Size: 17 1 482 144 156 144 181 4897
Fragments arranged by size:
4897 482 181 156 144 144 17 1
EcoRI G'AATT_C
Cuts at: 3146 3331 3709 3146
Size: 185 378 5459
Fragments arranged by size:
5459 378 185
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2749 2749
Size: 6022
FspI TGC'GCA
Cuts at: 4528 4528
Size: 6022
HaeII r_GCGC'y
Cuts at: 1678 2809 3124 5169 1678
Size: 1131 315 2045 2531
Fragments arranged by size:
2531 2045 1131 315
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2557 2589 2970 3002 4663
Size: 32 135 32 985 32 381 32 1661
Cuts at: 4663 4695 4737 4769 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1661 985 381 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3366 3612 669
Size: 988 281 1428 246 3079
Fragments arranged by size:
3079 1428 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6022
HpaI GTT'AAC
Cuts at: 3612 3612
Size: 6022
KpnI G_GTAC'C
Cuts at: 3347 3347
Size: 6022
MfeI C'AATT_G
Cuts at: 3621 3621
Size: 6022
MluI A'CGCG_T
Cuts at: 5898 5898
Size: 6022
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3237 3327 5136 5320 1305
Size: 1932 90 1809 184 2007
Fragments arranged by size:
2007 1932 1809 184 90
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4200
Fragments arranged by size:
4200 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2520 2526 3237 3980 4339 4498 518
Size: 648 1354 6 711 743 359 159 2042
Fragments arranged by size:
2042 1354 743 711 648 359 159 6
MspA1I CmG'CkG
Cuts at: 1606 3092 3103 3272 3438 4115 5263 5508 1606
Size: 1486 11 169 166 677 1148 245 2120
Fragments arranged by size:
2120 1486 1148 677 245 169 166 11
NaeI GCC'GGC
Cuts at: 2755 2816 2755
Size: 61 5961
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5470
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6022
NgoMIV G'CCGG_C
Cuts at: 2753 2814 2753
Size: 61 5961
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6022
NotI GC'GGCC_GC
Cuts at: 3469 3469
Size: 6022
NspI r_CATG'y
Cuts at: 1132 1229 2282 2895 3380 4925 1132
Size: 97 1053 613 485 1545 2229
Fragments arranged by size:
2229 1545 1053 613 485 97
PasI CC'CwG_GG
Cuts at: 1348 2717 2854 1348
Size: 1369 137 4516
Fragments arranged by size:
4516 1369 137
PciI A'CATG_T
Cuts at: 4921 4921
Size: 6022
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2097 2271 1108
Size: 989 174 4859
Fragments arranged by size:
4859 989 174
PmeI GTTT'AAAC
Cuts at: 3326 3326
Size: 6022
PmlI CAC'GTG
Cuts at: 2065 2065
Size: 6022
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4091 4123 5604 5636 4091
Size: 32 1481 32 4477
Fragments arranged by size:
4477 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2058 2702 3183 2058
Size: 644 481 4897
Fragments arranged by size:
4897 644 481
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6022
PsiI TTA'TAA
Cuts at: 3592 3592
Size: 6022
PspOMI G'GGCC_C
Cuts at: 2075 2557 2857 3001 2075
Size: 482 300 144 5096
Fragments arranged by size:
5096 482 300 144
PstI C_TGCA'G
Cuts at: 830 3374 830
Size: 2544 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6022
PvuII CAG'CTG
Cuts at: 3092 3272 3092
Size: 180 5842
SacI G_AGCT'C
Cuts at: 3255 3255
Size: 6022
SalI G'TCGA_C
Cuts at: 1936 3364 1936
Size: 1428 4594
SbfI CC_TGCA'GG
Cuts at: 3374 3374
Size: 6022
ScaI AGT'ACT
Cuts at: 4270 5982 4270
Size: 1712 4310
SfcI C'TryA_G
Cuts at: 826 1045 2316 2368 3187 3370 4505 5186 5377
Size: 219 1271 52 819 183 1135 681 191
Cuts at: 5377 826
Size: 1471
Fragments arranged by size:
1471 1271 1135 819 681 219 191 183
52
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6022
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6022
SmaI CCC'GGG
Cuts at: 1872 2540 3349 1872
Size: 668 809 4545
Fragments arranged by size:
4545 809 668
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4126 5027 5289 820
Size: 197 926 2183 901 262 1553
Fragments arranged by size:
2183 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3813 493
Size: 3320 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6022
SphI G_CATG'C
Cuts at: 1132 3380 1132
Size: 2248 3774
SspI AAT'ATT
Cuts at: 5 52 3946 5
Size: 47 3894 2081
Fragments arranged by size:
3894 2081 47
StuI AGG'CCT
Cuts at: 1517 2322 1517
Size: 805 5217
StyI C'CwwG_G
Cuts at: 513 1065 2125 3078 3309 3461 513
Size: 552 1060 953 231 152 3074
Fragments arranged by size:
3074 1060 953 552 231 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2138 2511 4049 4066 4219 4404 4849
Size: 1255 558 373 1538 17 153 185 445
Cuts at: 4849 325
Size: 1498
Fragments arranged by size:
1538 1498 1255 558 445 373 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2775 2886 4268
Size: 274 80 33 51 1443 798 111 1382
Cuts at: 4268 5980 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1382 798 274 138 111 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2409 2441 3012 3044 5982 6014 2409
Size: 32 571 32 2938 32 2417
Fragments arranged by size:
2938 2417 571 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6022
XbaI T'CTAG_A
Cuts at: 3358 3358
Size: 6022
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2551 2577 2644 2851 2941 3244 2551
Size: 26 67 207 90 303 5329
Fragments arranged by size:
5329 303 207 90 67 26
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6022
XmaI C'CCGG_G
Cuts at: 1870 2538 3347 1870
Size: 668 809 4545
Fragments arranged by size:
4545 809 668
XmnI GAAnn'nnTTC
Cuts at: 4151 4151
Size: 6022
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3830 276
Size: 53 83 186 936 2296 2468
Fragments arranged by size:
2468 2296 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PfoI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI