Restriction map for
pFN21AE1370
(Circular) (Six-base) MAPSORT of: pFN21AE1370.seq Check: 3082 from: 1 to: 5452
pFN21AE1370 5452 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3262 278
Size: 53 83 186 936 1726 2468
Fragments arranged by size:
2468 1726 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2795 1937
Size: 858 4594
Acc65I G'GTAC_C
Cuts at: 2773 2773
Size: 5452
AclI AA'CG_TT
Cuts at: 1563 3579 3952 1563
Size: 2016 373 3063
Fragments arranged by size:
3063 2016 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2603 3515 4899 5167 1632
Size: 971 912 1384 268 1917
Fragments arranged by size:
1917 1384 971 912 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5255
AflIII A'CryG_T
Cuts at: 1203 4351 5328 1203
Size: 3148 977 1327
Fragments arranged by size:
3148 1327 977
AgeI A'CCGG_T
Cuts at: 4283 4283
Size: 5452
AhdI GACnn_n'nnGTC
Cuts at: 4181 4181
Size: 5452
AleI CACnn'nnGTG
Cuts at: 1166 2120 2276 1166
Size: 954 156 4342
Fragments arranged by size:
4342 954 156
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5452
AlwNI CAG_nnn'CTG
Cuts at: 2068 3195 4767 2068
Size: 1127 1572 2753
Fragments arranged by size:
2753 1572 1127
ApaLI G'TGCA_C
Cuts at: 3509 4665 3509
Size: 1156 4296
ApoI r'AATT_y
Cuts at: 1493 2723 2761 2987 3139 5248 1493
Size: 1230 38 226 152 2109 1697
Fragments arranged by size:
2109 1697 1230 226 152 38
AseI AT'TA_AT
Cuts at: 160 1032 4006 160
Size: 872 2974 1606
Fragments arranged by size:
2974 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5452
AvaI C'yCGr_G
Cuts at: 1870 1943 2777 1870
Size: 73 834 4545
Fragments arranged by size:
4545 834 73
BamHI G'GATC_C
Cuts at: 2782 3149 2782
Size: 367 5085
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2104 2773 4228 618
Size: 325 217 627 221 96 669 1455 1842
Fragments arranged by size:
1842 1455 669 627 325 221 217 96
BanII G_rGCy'C
Cuts at: 1391 2079 2771 1391
Size: 688 692 4072
Fragments arranged by size:
4072 692 688
BbeI G_GCGC'C
Cuts at: 2012 2012
Size: 5452
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4481
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3643 3677 1903
Size: 34 1706 34 3678
Fragments arranged by size:
3678 1706 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3344 4554 3344
Size: 1210 4242
BclI T'GATC_A
Cuts at: 1548 2253 1548
Size: 705 4747
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4063 136
Size: 107 122 71 631 605 2391 1525
Fragments arranged by size:
2391 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5447 5447
Size: 5452
BlpI GC'TnA_GC
Cuts at: 2869 2869
Size: 5452
Bme1580I G_kGCm'C
Cuts at: 1790 2292 3513 4669 1790
Size: 502 1221 1156 2573
Fragments arranged by size:
2573 1221 1156 502
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5452
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2241 2655 3183
Size: 416 53 83 399 24 824 414 528
Cuts at: 3183 4141 442
Size: 958 1753
Fragments arranged by size:
1753 958 824 528 416 414 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5452
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1999 2497 4112 1239
Size: 760 498 1615 2579
Fragments arranged by size:
2579 1615 760 498
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2699 3577 4442 4740 2699
Size: 878 865 298 3411
Fragments arranged by size:
3411 878 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4115 882
Size: 617 2616 2219
Fragments arranged by size:
2616 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2274 3243 493
Size: 711 1070 969 2702
Fragments arranged by size:
2702 1070 969 711
BsaBI GATnn'nnATC
Cuts at: 5446 5446
Size: 5452
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 3259 3641 4276
Size: 53 83 186 936 476 1250 382 635
Cuts at: 4276 275
Size: 1451
Fragments arranged by size:
1451 1250 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3885 4283 4557 4704 5115 3885
Size: 398 274 147 411 4222
Fragments arranged by size:
4222 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2625 2655 1178
Size: 30 165 30 1222 30 3975
Fragments arranged by size:
3975 1222 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5452
BseYI C'CCAG_C
Cuts at: 4655 5151 4655
Size: 496 4956
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4879
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2112 2902 3663 4691 1540
Size: 275 186 111 790 761 1028 2301
Fragments arranged by size:
2301 1028 790 761 275 186 111
BsiHKAI G_wGCw'C
Cuts at: 2079 2134 2618 3513 3598 4669 2079
Size: 55 484 895 85 1071 2862
Fragments arranged by size:
2862 1071 895 484 85 55
BsmI GAATG_Cn'
Cuts at: 2963 3056 2963
Size: 93 5359
BsmBI CGTCTCn'nnnn_
Cuts at: 2599 2599
Size: 5452
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2134 2292 2618 2771 3513 3598
Size: 399 289 55 158 326 153 742 85
Cuts at: 3598 4669 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 326 289 158 153
85 55
BspEI T'CCGG_A
Cuts at: 5115 5115
Size: 5452
BspHI T'CATG_A
Cuts at: 3341 5071 3341
Size: 1730 3722
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2663 2807 844
Size: 1819 144 3489
Fragments arranged by size:
3489 1819 144
BsrBI CCG'CTC
Cuts at: 1394 1823 3339 1394
Size: 429 1516 3507
Fragments arranged by size:
3507 1516 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3947 4121 65
Size: 1183 2699 174 1396
Fragments arranged by size:
2699 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2107 4096 4283 1700
Size: 110 297 1989 187 2869
Fragments arranged by size:
2869 1989 297 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5452
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5452
BssSI C'ACGA_G
Cuts at: 2561 3512 4524 2561
Size: 951 1012 3489
Fragments arranged by size:
3489 1012 951
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5452
BstBI TT'CG_AA
Cuts at: 4256 4256
Size: 5452
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5452
BstXI CCAn_nnnn'nTGG
Cuts at: 2278 2278
Size: 5452
BstYI r'GATC_y
Cuts at: 1972 2173 2782 3149 3534 3551 5003 5089 5101
Size: 201 609 367 385 17 1452 86 12
Cuts at: 5101 5447 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 609 385 367 346 201 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3961
Fragments arranged by size:
3961 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2440 487
Size: 683 56 1214 3499
Fragments arranged by size:
3499 1214 683 56
BtsI GCAGTG_nn'
Cuts at: 2968 3762 3782 2968
Size: 794 20 4638
Fragments arranged by size:
4638 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5452
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5417
DraI TTT'AAA
Cuts at: 2756 2756
Size: 5452
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4459 809
Size: 507 3143 1802
Fragments arranged by size:
3143 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2097 2109 2667
Size: 54 1425 50 275 18 267 12 558
Cuts at: 2667 2899 3788 5417 8
Size: 232 889 1629 43
Fragments arranged by size:
1629 1425 889 558 275 267 232 54
50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2109 2899 1537
Size: 275 297 790 4090
Fragments arranged by size:
4090 790 297 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3382 1353
Size: 2029 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2071 4039 4413 4559 387
Size: 1064 138 482 1968 374 146 1280
Fragments arranged by size:
1968 1280 1064 482 374 146 138
EcoICRI GAG'CTC
Cuts at: 2077 2077
Size: 5452
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1999 2497 2603 3515 4112 4899 5167
Size: 393 367 498 106 912 597 787 268
Cuts at: 5167 1239
Size: 1524
Fragments arranged by size:
1524 912 787 597 498 393 367 268
106
EcoRI G'AATT_C
Cuts at: 2723 2761 3139 2723
Size: 38 378 5036
Fragments arranged by size:
5036 378 38
FspI TGC'GCA
Cuts at: 2196 3958 2196
Size: 1762 3690
HaeII r_GCGC'y
Cuts at: 1678 2012 4599 1678
Size: 334 2587 2531
Fragments arranged by size:
2587 2531 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4093 4125 4167 4199 1373
Size: 32 135 32 2521 32 42 32 2626
Fragments arranged by size:
2626 2521 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2796 3042 669
Size: 988 281 858 246 3079
Fragments arranged by size:
3079 988 858 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5452
HpaI GTT'AAC
Cuts at: 3042 3042
Size: 5452
KasI G'GCGC_C
Cuts at: 2008 2008
Size: 5452
KpnI G_GTAC'C
Cuts at: 2777 2777
Size: 5452
MfeI C'AATT_G
Cuts at: 3051 3051
Size: 5452
MluI A'CGCG_T
Cuts at: 5328 5328
Size: 5452
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2613 4566 4750 1305
Size: 1308 1953 184 2007
Fragments arranged by size:
2007 1953 1308 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2669 10
Size: 54 1425 343 837 2793
Fragments arranged by size:
2793 1425 837 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2120 2276 3410 3769 3928 518
Size: 648 954 156 1134 359 159 2042
Fragments arranged by size:
2042 1134 954 648 359 159 156
MspA1I CmG'CkG
Cuts at: 1606 2868 3545 4693 4938 1606
Size: 1262 677 1148 245 2120
Fragments arranged by size:
2120 1262 1148 677 245
NaeI GCC'GGC
Cuts at: 2109 2109
Size: 5452
NarI GG'CG_CC
Cuts at: 2009 2009
Size: 5452
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3961
Fragments arranged by size:
3961 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5452
NgoMIV G'CCGG_C
Cuts at: 2107 2107
Size: 5452
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5452
NotI GC'GGCC_GC
Cuts at: 2899 2899
Size: 5452
NspI r_CATG'y
Cuts at: 1132 1229 2309 2810 4355 1132
Size: 97 1080 501 1545 2229
Fragments arranged by size:
2229 1545 1080 501 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5452
PciI A'CATG_T
Cuts at: 4351 4351
Size: 5452
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2356 1108
Size: 1248 4204
PfoI T'CCnGG_A
Cuts at: 2015 2015
Size: 5452
PmeI GTTT'AAAC
Cuts at: 2756 2756
Size: 5452
PmlI CAC'GTG
Cuts at: 2274 2274
Size: 5452
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3521 3553 5034 5066 3521
Size: 32 1481 32 3907
Fragments arranged by size:
3907 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2450 1613
Size: 837 4615
PsiI TTA'TAA
Cuts at: 3022 3022
Size: 5452
PstI C_TGCA'G
Cuts at: 830 2804 830
Size: 1974 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5452
SacI G_AGCT'C
Cuts at: 2079 2079
Size: 5452
SalI G'TCGA_C
Cuts at: 1936 2794 1936
Size: 858 4594
SbfI CC_TGCA'GG
Cuts at: 2804 2804
Size: 5452
ScaI AGT'ACT
Cuts at: 3700 5412 3700
Size: 1712 3740
SfcI C'TryA_G
Cuts at: 826 1045 2800 3935 4616 4807 826
Size: 219 1755 1135 681 191 1471
Fragments arranged by size:
1755 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2010 2010
Size: 5452
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5452
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5452
SmaI CCC'GGG
Cuts at: 1872 2779 1872
Size: 907 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2714 3556 4457 4719 820
Size: 197 926 771 842 901 262 1553
Fragments arranged by size:
1553 926 901 842 771 262 197
SnaBI TAC'GTA
Cuts at: 493 3243 493
Size: 2750 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5452
SphI G_CATG'C
Cuts at: 1132 2810 1132
Size: 1678 3774
SspI AAT'ATT
Cuts at: 5 52 3376 5
Size: 47 3324 2081
Fragments arranged by size:
3324 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5452
StyI C'CwwG_G
Cuts at: 513 1065 2004 2100 2891 513
Size: 552 939 96 791 3074
Fragments arranged by size:
3074 939 791 552 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2266 2690 3479 3496 3649 3834 4279
Size: 1255 686 424 789 17 153 185 445
Cuts at: 4279 325
Size: 1498
Fragments arranged by size:
1498 1255 789 686 445 424 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2557 3698 5410
Size: 274 80 33 51 1443 580 1141 1712
Cuts at: 5410 96
Size: 138
Fragments arranged by size:
1712 1443 1141 580 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2625 2657 5412 5444 2625
Size: 32 2755 32 2633
Fragments arranged by size:
2755 2633 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5452
XbaI T'CTAG_A
Cuts at: 2788 2788
Size: 5452
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2185 2185
Size: 5452
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5452
XmaI C'CCGG_G
Cuts at: 1870 2777 1870
Size: 907 4545
XmnI GAAnn'nnTTC
Cuts at: 2024 3581 2024
Size: 1557 3895
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3260 276
Size: 53 83 186 936 1726 2468
Fragments arranged by size:
2468 1726 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PstI PvuI SacI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI
EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI
SapI SexAI SfiI SrfI SwaI