Restriction map for
pFN21AE1368
(Circular) (Six-base) MAPSORT of: pFN21AE1368.seq Check: 1806 from: 1 to: 6721
pFN21AE1368 6721 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2185 2500 2681 2991 3381 2185
Size: 315 181 310 390 5525
Fragments arranged by size:
5525 390 315 310 181
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4531 278
Size: 53 83 186 936 2995 2468
Fragments arranged by size:
2995 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2096 2466 4064 1937
Size: 159 370 1598 4594
Fragments arranged by size:
4594 1598 370 159
Acc65I G'GTAC_C
Cuts at: 4042 4042
Size: 6721
AclI AA'CG_TT
Cuts at: 1563 4848 5221 1563
Size: 3285 373 3063
Fragments arranged by size:
3285 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2585 2666 3446 4784 6168 6436 1632
Size: 953 81 780 1338 1384 268 1917
Fragments arranged by size:
1917 1384 1338 953 780 268 81
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6524
AflIII A'CryG_T
Cuts at: 1203 5620 6597 1203
Size: 4417 977 1327
Fragments arranged by size:
4417 1327 977
AgeI A'CCGG_T
Cuts at: 5552 5552
Size: 6721
AhdI GACnn_n'nnGTC
Cuts at: 5450 5450
Size: 6721
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6721
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2792 3082 3701 1184
Size: 1608 290 619 4204
Fragments arranged by size:
4204 1608 619 290
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2210 2242 2423 2455 2888 2909 2920 2941 2210
Size: 32 181 32 433 21 11 21 5990
Fragments arranged by size:
5990 433 181 32 32 21 21 11
AlwNI CAG_nnn'CTG
Cuts at: 2209 2659 2670 4464 6036 2209
Size: 450 11 1794 1572 2894
Fragments arranged by size:
2894 1794 1572 450 11
ApaI G_GGCC'C
Cuts at: 3193 3659 3193
Size: 466 6255
ApaLI G'TGCA_C
Cuts at: 4778 5934 4778
Size: 1156 5565
ApoI r'AATT_y
Cuts at: 1493 3101 4030 4256 4408 6517 1493
Size: 1608 929 226 152 2109 1697
Fragments arranged by size:
2109 1697 1608 929 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5275 160
Size: 872 4243 1606
Fragments arranged by size:
4243 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6721
AvaI C'yCGr_G
Cuts at: 1870 1943 2697 3192 3241 3518 3650 4046 1870
Size: 73 754 495 49 277 132 396 4545
Fragments arranged by size:
4545 754 495 396 277 132 73 49
AvrII C'CTAG_G
Cuts at: 3308 3308
Size: 6721
BamHI G'GATC_C
Cuts at: 2784 4051 4418 2784
Size: 1267 367 5087
Fragments arranged by size:
5087 1267 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2262 2693 3338 3866 4042
Size: 325 217 627 475 431 645 528 176
Cuts at: 4042 5497 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 645 627 528 475 431 325
217 176
BanII G_rGCy'C
Cuts at: 1391 2327 2748 2823 2880 2949 3193 3230 3387
Size: 936 421 75 57 69 244 37 157
Cuts at: 3387 3659 4040 1391
Size: 272 381 4072
Fragments arranged by size:
4072 936 421 381 272 244 157 75
69 57 37
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3451 928
Size: 971 1552 4198
Fragments arranged by size:
4198 1552 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4912 4946 1903
Size: 34 50 34 2891 34 3678
Fragments arranged by size:
3678 2891 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4613 5823 4613
Size: 1210 5511
BclI T'GATC_A
Cuts at: 1548 2661 1548
Size: 1113 5608
BfrBI ATG'CAT
Cuts at: 3353 3353
Size: 6721
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3494 5332
Size: 107 122 71 631 605 337 1485 1838
Cuts at: 5332 136
Size: 1525
Fragments arranged by size:
1838 1525 1485 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 6716 6716
Size: 6721
BlpI GC'TnA_GC
Cuts at: 2678 3806 4138 2678
Size: 1128 332 5261
Fragments arranged by size:
5261 1128 332
Bme1580I G_kGCm'C
Cuts at: 1790 2698 3193 3199 3659 3885 4782 5938 1790
Size: 908 495 6 460 226 897 1156 2573
Fragments arranged by size:
2573 1156 908 897 495 460 226 6
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6721
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2104 3187 3699
Size: 416 53 83 399 24 687 1083 512
Cuts at: 3699 4452 5410 442
Size: 753 958 1753
Fragments arranged by size:
1753 1083 958 753 687 512 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6721
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2732 2845 2876 5381 1239
Size: 1493 113 31 2505 2579
Fragments arranged by size:
2579 2505 1493 113 31
Bpu10I CC'TnA_GC
Cuts at: 3482 3482
Size: 6721
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4846 5711 6009 4846
Size: 865 298 5558
Fragments arranged by size:
5558 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3121 4000 5384 882
Size: 617 1622 879 1384 2219
Fragments arranged by size:
2219 1622 1384 879 617
BsaAI yAC'GTr
Cuts at: 493 1204 2130 4512 493
Size: 711 926 2382 2702
Fragments arranged by size:
2702 2382 926 711
BsaBI GATnn'nnATC
Cuts at: 6715 6715
Size: 6721
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2027 2052 4528 4910
Size: 53 83 186 936 494 25 2476 382
Cuts at: 4910 5545 275
Size: 635 1451
Fragments arranged by size:
2476 1451 936 635 494 382 186 83
53 25
BsaWI w'CCGG_w
Cuts at: 5154 5552 5826 5973 6384 5154
Size: 398 274 147 411 5491
Fragments arranged by size:
5491 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2309 2339 2395 2423 2425
Size: 30 165 30 906 30 56 28 2
Cuts at: 2425 2453 2909 2939 1178
Size: 28 456 30 4960
Fragments arranged by size:
4960 906 456 165 56 30 30 30
30 28 28 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2065 2930 2933 2936 1186
Size: 879 865 3 3 4971
Fragments arranged by size:
4971 879 865 3 3
BseYI C'CCAG_C
Cuts at: 2212 2301 2358 2610 2704 2886 3089 3229 3413
Size: 89 57 252 94 182 203 140 184
Cuts at: 3413 3973 5924 6420 2212
Size: 560 1951 496 2513
Fragments arranged by size:
2513 1951 560 496 252 203 184 182
140 94 89 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3149 3254 3575 1163
Size: 573 1413 105 321 4309
Fragments arranged by size:
4309 1413 573 321 105
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3361 4171 4932 5960 1540
Size: 275 186 1360 810 761 1028 2301
Fragments arranged by size:
2301 1360 1028 810 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2496 2619 2679 2748 2880 2949 3387 4782 4867
Size: 123 60 69 132 69 438 1395 85
Cuts at: 4867 5938 2496
Size: 1071 3279
Fragments arranged by size:
3279 1395 1071 438 132 123 85 69
69 60
BsmI GAATG_Cn'
Cuts at: 4232 4325 4232
Size: 93 6628
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2327 2496 2619 2679 2698 2748 2823
Size: 399 537 169 123 60 19 50 75
Cuts at: 2823 2880 2949 3193 3199 3230 3387 3659 3885
Size: 57 69 244 6 31 157 272 226
Cuts at: 3885 4040 4782 4867 5938 1391
Size: 155 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 537 399 272 244 226
169 157 155 123 85 75 69 60
57 50 31 19 6
BspEI T'CCGG_A
Cuts at: 6384 6384
Size: 6721
BspHI T'CATG_A
Cuts at: 4610 6340 4610
Size: 1730 4991
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2185 2500 2681 2745 2991 3381 3903 4076
Size: 1341 315 181 64 246 390 522 173
Cuts at: 4076 844
Size: 3489
Fragments arranged by size:
3489 1341 522 390 315 246 181 173
64
BsrBI CCG'CTC
Cuts at: 1394 1823 4608 1394
Size: 429 2785 3507
Fragments arranged by size:
3507 2785 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3138 5216 5390 65
Size: 1183 1890 2078 174 1396
Fragments arranged by size:
2078 1890 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2029 2537 2802 2975 3699 3843 5365
Size: 110 219 508 265 173 724 144 1522
Cuts at: 5365 5552 1700
Size: 187 2869
Fragments arranged by size:
2869 1522 724 508 265 219 187 173
144 110
BsrGI T'GTAC_A
Cuts at: 96 3135 96
Size: 3039 3682
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6721
BssSI C'ACGA_G
Cuts at: 4781 5793 4781
Size: 1012 5709
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2209 3345 794
Size: 1415 1136 4170
Fragments arranged by size:
4170 1415 1136
BstBI TT'CG_AA
Cuts at: 5525 5525
Size: 6721
BstEII G'GTnAC_C
Cuts at: 1184 2368 1184
Size: 1184 5537
BstXI CCAn_nnnn'nTGG
Cuts at: 2610 2610
Size: 6721
BstYI r'GATC_y
Cuts at: 1972 2784 3155 4051 4418 4803 4820 6272 6358
Size: 812 371 896 367 385 17 1452 86
Cuts at: 6358 6370 6716 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 896 812 385 371 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2930 2930
Size: 6721
BtgI C'CryG_G
Cuts at: 513 1065 2067 2183 2479 3330 513
Size: 552 1002 116 296 851 3904
Fragments arranged by size:
3904 1002 851 552 296 116
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3806 3913 487
Size: 683 56 2580 107 3295
Fragments arranged by size:
3295 2580 683 107 56
BtsI GCAGTG_nn'
Cuts at: 3504 3694 3782 4014 4237 5031 5051 3504
Size: 190 88 232 223 794 20 5174
Fragments arranged by size:
5174 794 232 223 190 88 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6721
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3359 3394 529
Size: 35 2795 35 3856
Fragments arranged by size:
3856 2795 35 35
DraI TTT'AAA
Cuts at: 4025 4025
Size: 6721
DraIII CAC_nnn'GTG
Cuts at: 3345 3345
Size: 6721
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3365 5728 809
Size: 507 2049 2363 1802
Fragments arranged by size:
2363 2049 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2977 3501 3580
Size: 54 1425 50 275 18 1147 524 79
Cuts at: 3580 4168 5057 6686 8
Size: 588 889 1629 43
Fragments arranged by size:
1629 1425 1147 889 588 524 275 79
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4168 1537
Size: 275 2356 4090
Fragments arranged by size:
4090 2356 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2087 2249 4651 1353
Size: 734 162 2402 3423
Fragments arranged by size:
3423 2402 734 162
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2531 3188 5308 5682 5828 387
Size: 1064 138 942 657 2120 374 146 1280
Fragments arranged by size:
2120 1280 1064 942 657 374 146 138
EcoICRI GAG'CTC
Cuts at: 2746 2878 2947 3385 2746
Size: 132 69 438 6082
Fragments arranged by size:
6082 438 132 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2585 2666 2732 2845 2876 3446 4784
Size: 393 953 81 66 113 31 570 1338
Cuts at: 4784 5381 6168 6436 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1338 953 787 597 570 393 268
113 81 66 31
EcoNI CCTnn'n_nnAGG
Cuts at: 2273 3278 3989 2273
Size: 1005 711 5005
Fragments arranged by size:
5005 1005 711
EcoO109I rG'GnC_Cy
Cuts at: 2484 3036 3313 3655 3656 3748 3912 2484
Size: 552 277 342 1 92 164 5293
Fragments arranged by size:
5293 552 342 277 164 92 1
EcoRI G'AATT_C
Cuts at: 4030 4408 4030
Size: 378 6343
FseI GG_CCGG'CC
Cuts at: 2979 2979
Size: 6721
FspI TGC'GCA
Cuts at: 5227 5227
Size: 6721
HaeII r_GCGC'y
Cuts at: 1678 3029 3489 5868 1678
Size: 1351 460 2379 2531
Fragments arranged by size:
2531 2379 1351 460
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2867 2899 3935 3967 5362
Size: 32 135 32 1295 32 1036 32 1395
Cuts at: 5362 5394 5436 5468 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1395 1295 1036 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4065 4311 669
Size: 988 281 2127 246 3079
Fragments arranged by size:
3079 2127 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6721
HpaI GTT'AAC
Cuts at: 4311 4311
Size: 6721
KpnI G_GTAC'C
Cuts at: 4046 4046
Size: 6721
MfeI C'AATT_G
Cuts at: 4320 4320
Size: 6721
MluI A'CGCG_T
Cuts at: 6597 6597
Size: 6721
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5835 6019 1305
Size: 4530 184 2007
Fragments arranged by size:
4530 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3503 10
Size: 54 1425 343 1671 3228
Fragments arranged by size:
3228 1671 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4007 4679 5038 5197 518
Size: 648 2841 672 359 159 2042
Fragments arranged by size:
2841 2042 672 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2069 2104 2421 2610 2635 2667 2864 3081
Size: 463 35 317 189 25 32 197 217
Cuts at: 3081 3669 3802 4137 4814 5962 6207 1606
Size: 588 133 335 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 588 463 335 317 245
217 197 189 133 35 32 25
NaeI GCC'GGC
Cuts at: 2031 2804 2977 3701 3845 2031
Size: 773 173 724 144 4907
Fragments arranged by size:
4907 773 724 173 144
NcoI C'CATG_G
Cuts at: 513 1065 2479 513
Size: 552 1414 4755
Fragments arranged by size:
4755 1414 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6721
NgoMIV G'CCGG_C
Cuts at: 2029 2802 2975 3699 3843 2029
Size: 773 173 724 144 4907
Fragments arranged by size:
4907 773 724 173 144
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6721
NotI GC'GGCC_GC
Cuts at: 4168 4168
Size: 6721
NsiI A_TGCA'T
Cuts at: 3355 3355
Size: 6721
NspI r_CATG'y
Cuts at: 1132 1229 4079 5624 1132
Size: 97 2850 1545 2229
Fragments arranged by size:
2850 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2796 1348
Size: 1448 5273
PciI A'CATG_T
Cuts at: 5620 5620
Size: 6721
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3105 3262 3758 1108
Size: 1997 157 496 4071
Fragments arranged by size:
4071 1997 496 157
PfoI T'CCnGG_A
Cuts at: 2046 2268 2287 2892 2046
Size: 222 19 605 5875
Fragments arranged by size:
5875 605 222 19
PmeI GTTT'AAAC
Cuts at: 4025 4025
Size: 6721
PmlI CAC'GTG
Cuts at: 2130 2130
Size: 6721
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2423 2455 2909 2941 3596 3628 4790 4822 6303
Size: 32 454 32 655 32 1162 32 1481
Cuts at: 6303 6335 2423
Size: 32 2809
Fragments arranged by size:
2809 1481 1162 655 454 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2484 3313 3748 2484
Size: 829 435 5457
Fragments arranged by size:
5457 829 435
PshAI GACnn'nnGTC
Cuts at: 1613 2655 1613
Size: 1042 5679
PsiI TTA'TAA
Cuts at: 4291 4291
Size: 6721
PspOMI G'GGCC_C
Cuts at: 3189 3655 3189
Size: 466 6255
PstI C_TGCA'G
Cuts at: 830 2253 2988 3153 3795 3865 4073 830
Size: 1423 735 165 642 70 208 3478
Fragments arranged by size:
3478 1423 735 642 208 165 70
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6721
PvuII CAG'CTG
Cuts at: 2635 2667 2635
Size: 32 6689
SacI G_AGCT'C
Cuts at: 2748 2880 2949 3387 2748
Size: 132 69 438 6082
Fragments arranged by size:
6082 438 132 69
SacII CC_GC'GG
Cuts at: 2070 2070
Size: 6721
SalI G'TCGA_C
Cuts at: 1936 4063 1936
Size: 2127 4594
SapI GCTCTTCn'nnn_
Cuts at: 2087 2249 2087
Size: 162 6559
SbfI CC_TGCA'GG
Cuts at: 3865 4073 3865
Size: 208 6513
ScaI AGT'ACT
Cuts at: 4969 6681 4969
Size: 1712 5009
SexAI A'CCwGG_T
Cuts at: 2274 2274
Size: 6721
SfcI C'TryA_G
Cuts at: 826 1045 2249 2984 3149 3791 3861 4069 5204
Size: 219 1204 735 165 642 70 208 1135
Cuts at: 5204 5885 6076 826
Size: 681 191 1471
Fragments arranged by size:
1471 1204 1135 735 681 642 219 208
191 165 70
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6721
SgrAI Cr'CCGG_yG
Cuts at: 1700 2029 1700
Size: 329 6392
SmaI CCC'GGG
Cuts at: 1872 2699 3194 3243 3520 4048 1872
Size: 827 495 49 277 528 4545
Fragments arranged by size:
4545 827 528 495 277 49
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4825 5726 5988 820
Size: 197 926 2882 901 262 1553
Fragments arranged by size:
2882 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4512 493
Size: 4019 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6721
SphI G_CATG'C
Cuts at: 1132 4079 1132
Size: 2947 3774
SrfI GCCC'GGGC
Cuts at: 3194 3194
Size: 6721
SspI AAT'ATT
Cuts at: 5 52 4645 5
Size: 47 4593 2081
Fragments arranged by size:
4593 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6721
StyI C'CwwG_G
Cuts at: 513 1065 2472 2479 3308 4160 513
Size: 552 1407 7 829 852 3074
Fragments arranged by size:
3074 1407 852 829 552 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2350 2899 3194 3634 4748 4765 4918
Size: 1255 770 549 295 440 1114 17 153
Cuts at: 4918 5103 5548 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1114 770 549 445 440 295
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3135 4967 6679
Size: 274 80 33 51 1443 1158 1832 1712
Cuts at: 6679 96
Size: 138
Fragments arranged by size:
1832 1712 1443 1158 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2114 2146 2346 2378 6681 6713 2114
Size: 32 200 32 4303 32 2122
Fragments arranged by size:
4303 2122 200 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2276 1316
Size: 960 5761
XbaI T'CTAG_A
Cuts at: 4057 4057
Size: 6721
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3328 3496 3328
Size: 168 6553
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6721
XmaI C'CCGG_G
Cuts at: 1870 2697 3192 3241 3518 4046 1870
Size: 827 495 49 277 528 4545
Fragments arranged by size:
4545 827 528 495 277 49
XmnI GAAnn'nnTTC
Cuts at: 4850 4850
Size: 6721
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4529 276
Size: 53 83 186 936 2995 2468
Fragments arranged by size:
2995 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BbvCI BplI BsiWI BsmBI
BstZ17I EcoRV FalI FspAI KasI NarI NruI PacI
PspXI PsrI RsrII SanDI SfiI SfoI SwaI