Restriction map for
pFN21AE1352
(Circular) (Six-base) MAPSORT of: pFN21AE1352.seq Check: 6065 from: 1 to: 5701
pFN21AE1352 5701 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3511 278
Size: 53 83 186 936 1975 2468
Fragments arranged by size:
2468 1975 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3044 1937
Size: 1107 4594
Acc65I G'GTAC_C
Cuts at: 3022 3022
Size: 5701
AclI AA'CG_TT
Cuts at: 1563 3828 4201 1563
Size: 2265 373 3063
Fragments arranged by size:
3063 2265 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3764 5148 5416 1632
Size: 2132 1384 268 1917
Fragments arranged by size:
2132 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5504
AflIII A'CryG_T
Cuts at: 1203 2718 4600 5577 1203
Size: 1515 1882 977 1327
Fragments arranged by size:
1882 1515 1327 977
AgeI A'CCGG_T
Cuts at: 4532 4532
Size: 5701
AhdI GACnn_n'nnGTC
Cuts at: 4430 4430
Size: 5701
AleI CACnn'nnGTG
Cuts at: 1166 2651 1166
Size: 1485 4216
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5701
AlwNI CAG_nnn'CTG
Cuts at: 3444 5016 3444
Size: 1572 4129
ApaLI G'TGCA_C
Cuts at: 3758 4914 3758
Size: 1156 4545
ApoI r'AATT_y
Cuts at: 1493 2329 3010 3236 3388 5497 1493
Size: 836 681 226 152 2109 1697
Fragments arranged by size:
2109 1697 836 681 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4255 160
Size: 872 3223 1606
Fragments arranged by size:
3223 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5701
AvaI C'yCGr_G
Cuts at: 1870 1943 2865 3026 1870
Size: 73 922 161 4545
Fragments arranged by size:
4545 922 161 73
BamHI G'GATC_C
Cuts at: 3031 3398 3031
Size: 367 5334
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2044 2318 3022 4477 618
Size: 325 217 627 257 274 704 1455 1842
Fragments arranged by size:
1842 1455 704 627 325 274 257 217
BanII G_rGCy'C
Cuts at: 1391 2073 3020 1391
Size: 682 947 4072
Fragments arranged by size:
4072 947 682
BbeI G_GCGC'C
Cuts at: 2048 2048
Size: 5701
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2697 2892 928
Size: 971 798 195 3737
Fragments arranged by size:
3737 971 798 195
BbvCI CC'TCA_GC
Cuts at: 2907 2907
Size: 5701
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2314 2329 2348 2363 3892 3926 1903
Size: 34 377 15 19 15 1529 34 3678
Fragments arranged by size:
3678 1529 377 34 34 19 15 15
BciVI GTATCCnnnnn_n'
Cuts at: 2855 3593 4803 2855
Size: 738 1210 3753
Fragments arranged by size:
3753 1210 738
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5701
BfrBI ATG'CAT
Cuts at: 2530 2530
Size: 5701
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4312 136
Size: 107 122 71 631 605 2640 1525
Fragments arranged by size:
2640 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5696 5696
Size: 5701
BlpI GC'TnA_GC
Cuts at: 3118 3118
Size: 5701
Bme1580I G_kGCm'C
Cuts at: 1790 3762 4918 1790
Size: 1972 1156 2573
Fragments arranged by size:
2573 1972 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5701
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3432 4390 442
Size: 416 53 83 399 24 2015 958 1753
Fragments arranged by size:
2015 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5701
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2652 2689 4361 1239
Size: 1413 37 1672 2579
Fragments arranged by size:
2579 1672 1413 37
Bpu10I CC'TnA_GC
Cuts at: 2171 2907 2171
Size: 736 4965
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3826 4691 4989 3826
Size: 865 298 4538
Fragments arranged by size:
4538 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2596 4364 882
Size: 617 1097 1768 2219
Fragments arranged by size:
2219 1768 1097 617
BsaAI yAC'GTr
Cuts at: 493 1204 3492 493
Size: 711 2288 2702
Fragments arranged by size:
2702 2288 711
BsaBI GATnn'nnATC
Cuts at: 5695 5695
Size: 5701
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2045 2100 3508 3890
Size: 53 83 186 936 512 55 1408 382
Cuts at: 3890 4525 275
Size: 635 1451
Fragments arranged by size:
1451 1408 936 635 512 382 186 83
55 53
BsaWI w'CCGG_w
Cuts at: 2781 4134 4532 4806 4953 5364 2781
Size: 1353 398 274 147 411 3118
Fragments arranged by size:
3118 1353 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5476
Fragments arranged by size:
5476 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2686 2924 2948 1186
Size: 1500 238 24 3939
Fragments arranged by size:
3939 1500 238 24
BseYI C'CCAG_C
Cuts at: 2011 2026 4904 5400 2011
Size: 15 2878 496 2312
Fragments arranged by size:
2878 2312 496 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2078 2288 1163
Size: 573 342 210 4576
Fragments arranged by size:
4576 573 342 210
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3151 3912 4940 1540
Size: 275 186 1150 761 1028 2301
Fragments arranged by size:
2301 1150 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3762 3847 4918 3762
Size: 85 1071 4545
Fragments arranged by size:
4545 1071 85
BsmI GAATG_Cn'
Cuts at: 3212 3305 3212
Size: 93 5608
BsmBI CGTCTCn'nnnn_
Cuts at: 2132 2132
Size: 5701
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 3020 3762 3847 4918 1391
Size: 399 283 947 742 85 1071 2174
Fragments arranged by size:
2174 1071 947 742 399 283 85
BspEI T'CCGG_A
Cuts at: 5364 5364
Size: 5701
BspHI T'CATG_A
Cuts at: 3590 5320 3590
Size: 1730 3971
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3056 844
Size: 2212 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3588 1394
Size: 429 1765 3507
Fragments arranged by size:
3507 1765 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2408 2632 4196 4370 65
Size: 1183 1160 224 1564 174 1396
Fragments arranged by size:
1564 1396 1183 1160 224 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2096 2108 2321 4345 4532 1700
Size: 110 286 12 213 2024 187 2869
Fragments arranged by size:
2869 2024 286 213 187 110 12
BsrGI T'GTAC_A
Cuts at: 96 2442 96
Size: 2346 3355
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5701
BssSI C'ACGA_G
Cuts at: 3761 4773 3761
Size: 1012 4689
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5701
BstBI TT'CG_AA
Cuts at: 4505 4505
Size: 5701
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5701
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2080 2011
Size: 69 5632
BstYI r'GATC_y
Cuts at: 1972 2194 3031 3398 3783 3800 5252 5338 5350
Size: 222 837 367 385 17 1452 86 12
Cuts at: 5350 5696 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 837 385 367 346 222 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2052 513
Size: 552 987 4162
Fragments arranged by size:
4162 987 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2332 487
Size: 683 56 1106 3856
Fragments arranged by size:
3856 1106 683 56
BtsI GCAGTG_nn'
Cuts at: 2183 3217 4011 4031 2183
Size: 1034 794 20 3853
Fragments arranged by size:
3853 1034 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5701
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2734 2769 529
Size: 35 2170 35 3461
Fragments arranged by size:
3461 2170 35 35
DraI TTT'AAA
Cuts at: 3005 3005
Size: 5701
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4708 809
Size: 507 3392 1802
Fragments arranged by size:
3392 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2094 2403 3148
Size: 54 1425 50 275 18 264 309 745
Cuts at: 3148 4037 5666 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 745 309 275 264 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3148 1537
Size: 275 1336 4090
Fragments arranged by size:
4090 1336 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2144 3631 1353
Size: 791 1487 3423
Fragments arranged by size:
3423 1487 791
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4288 4662 4808 387
Size: 1064 138 2699 374 146 1280
Fragments arranged by size:
2699 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2652 2689 3764 4361 5148 5416 1239
Size: 393 1020 37 1075 597 787 268 1524
Fragments arranged by size:
1524 1075 1020 787 597 393 268 37
EcoNI CCTnn'n_nnAGG
Cuts at: 2491 2491
Size: 5701
EcoRI G'AATT_C
Cuts at: 3010 3388 3010
Size: 378 5323
FspI TGC'GCA
Cuts at: 4207 4207
Size: 5701
HaeII r_GCGC'y
Cuts at: 1678 2048 4848 1678
Size: 370 2800 2531
Fragments arranged by size:
2800 2531 370
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2576 2608 4342 4374 4416
Size: 32 135 32 1004 32 1734 32 42
Cuts at: 4416 4448 1373
Size: 32 2626
Fragments arranged by size:
2626 1734 1004 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3045 3291 669
Size: 988 281 1107 246 3079
Fragments arranged by size:
3079 1107 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5701
HpaI GTT'AAC
Cuts at: 3291 3291
Size: 5701
KasI G'GCGC_C
Cuts at: 2044 2044
Size: 5701
KpnI G_GTAC'C
Cuts at: 3026 3026
Size: 5701
MfeI C'AATT_G
Cuts at: 3300 3300
Size: 5701
MluI A'CGCG_T
Cuts at: 2718 5577 2718
Size: 2859 2842
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4815 4999 1305
Size: 3510 184 2007
Fragments arranged by size:
3510 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2405 10
Size: 54 1425 343 573 3306
Fragments arranged by size:
3306 1425 573 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2651 3659 4018 4177 518
Size: 648 1485 1008 359 159 2042
Fragments arranged by size:
2042 1485 1008 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3117 3794 4942 5187 1606
Size: 1511 677 1148 245 2120
Fragments arranged by size:
2120 1511 1148 677 245
NaeI GCC'GGC
Cuts at: 2098 2098
Size: 5701
NarI GG'CG_CC
Cuts at: 2045 2045
Size: 5701
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5149
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5701
NgoMIV G'CCGG_C
Cuts at: 2096 2096
Size: 5701
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5701
NotI GC'GGCC_GC
Cuts at: 3148 3148
Size: 5701
NsiI A_TGCA'T
Cuts at: 2532 2532
Size: 5701
NspI r_CATG'y
Cuts at: 1132 1229 2426 2534 3059 4604 1132
Size: 97 1197 108 525 1545 2229
Fragments arranged by size:
2229 1545 1197 525 108 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5701
PciI A'CATG_T
Cuts at: 4600 4600
Size: 5701
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5701
PmeI GTTT'AAAC
Cuts at: 3005 3005
Size: 5701
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3770 3802 5283 5315 3770
Size: 32 1481 32 4156
Fragments arranged by size:
4156 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5701
PsiI TTA'TAA
Cuts at: 3271 3271
Size: 5701
PstI C_TGCA'G
Cuts at: 830 2190 3053 830
Size: 1360 863 3478
Fragments arranged by size:
3478 1360 863
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5701
SalI G'TCGA_C
Cuts at: 1936 3043 1936
Size: 1107 4594
SbfI CC_TGCA'GG
Cuts at: 3053 3053
Size: 5701
ScaI AGT'ACT
Cuts at: 3949 5661 3949
Size: 1712 3989
SexAI A'CCwGG_T
Cuts at: 2577 2577
Size: 5701
SfcI C'TryA_G
Cuts at: 826 1045 2186 3049 4184 4865 5056 826
Size: 219 1141 863 1135 681 191 1471
Fragments arranged by size:
1471 1141 1135 863 681 219 191
SfoI GGC'GCC
Cuts at: 2046 2046
Size: 5701
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5701
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5701
SmaI CCC'GGG
Cuts at: 1872 3028 1872
Size: 1156 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3805 4706 4968 820
Size: 197 926 1862 901 262 1553
Fragments arranged by size:
1862 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3492 493
Size: 2999 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5701
SphI G_CATG'C
Cuts at: 1132 2534 3059 1132
Size: 1402 525 3774
Fragments arranged by size:
3774 1402 525
SspI AAT'ATT
Cuts at: 5 52 2359 3625 5
Size: 47 2307 1266 2081
Fragments arranged by size:
2307 2081 1266 47
StuI AGG'CCT
Cuts at: 1517 2538 1517
Size: 1021 4680
StyI C'CwwG_G
Cuts at: 513 1065 3140 513
Size: 552 2075 3074
Fragments arranged by size:
3074 2075 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3728 3745 3898 4083 4528 325
Size: 1255 2148 17 153 185 445 1498
Fragments arranged by size:
2148 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2442 3947 5659
Size: 274 80 33 51 1443 465 1505 1712
Cuts at: 5659 96
Size: 138
Fragments arranged by size:
1712 1505 1443 465 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5661 5693 5661
Size: 32 5669
Tth111I GACn'n_nGTC
Cuts at: 1316 2387 1316
Size: 1071 4630
XbaI T'CTAG_A
Cuts at: 3037 3037
Size: 5701
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5701
XmaI C'CCGG_G
Cuts at: 1870 3026 1870
Size: 1156 4545
XmnI GAAnn'nnTTC
Cuts at: 3830 3830
Size: 5701
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3509 276
Size: 53 83 186 936 1975 2468
Fragments arranged by size:
2468 1975 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BplI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI
FseI FspAI NruI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SfiI SrfI SwaI XcmI