Restriction map for
pFN21AE1348
(Circular) (Six-base) MAPSORT of: pFN21AE1348.seq Check: 1498 from: 1 to: 6121
pFN21AE1348 6121 bp
With 182 enzymes: SgfI *
December 17, 2009 17:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3931 278
Size: 53 83 186 936 2395 2468
Fragments arranged by size:
2468 2395 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3464 1937
Size: 1527 4594
Acc65I G'GTAC_C
Cuts at: 3442 3442
Size: 6121
AclI AA'CG_TT
Cuts at: 1563 4248 4621 1563
Size: 2685 373 3063
Fragments arranged by size:
3063 2685 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3239 4184 5568 5836 1632
Size: 1607 945 1384 268 1917
Fragments arranged by size:
1917 1607 1384 945 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5924
AflIII A'CryG_T
Cuts at: 1203 3288 5020 5997 1203
Size: 2085 1732 977 1327
Fragments arranged by size:
2085 1732 1327 977
AgeI A'CCGG_T
Cuts at: 4952 4952
Size: 6121
AhdI GACnn_n'nnGTC
Cuts at: 4850 4850
Size: 6121
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6121
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6121
AlwNI CAG_nnn'CTG
Cuts at: 2399 3864 5436 2399
Size: 1465 1572 3084
Fragments arranged by size:
3084 1572 1465
ApaLI G'TGCA_C
Cuts at: 4178 5334 4178
Size: 1156 4965
ApoI r'AATT_y
Cuts at: 1493 3198 3430 3656 3808 5917 1493
Size: 1705 232 226 152 2109 1697
Fragments arranged by size:
2109 1705 1697 232 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2742 4675 160
Size: 872 1710 1933 1606
Fragments arranged by size:
1933 1710 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
AvaI C'yCGr_G
Cuts at: 1870 1943 3446 1870
Size: 73 1503 4545
Fragments arranged by size:
4545 1503 73
BamHI G'GATC_C
Cuts at: 2554 3451 3818 2554
Size: 897 367 4857
Fragments arranged by size:
4857 897 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2360 3442 4897 618
Size: 325 217 627 573 1082 1455 1842
Fragments arranged by size:
1842 1455 1082 627 573 325 217
BanII G_rGCy'C
Cuts at: 1391 2031 3440 1391
Size: 640 1409 4072
Fragments arranged by size:
4072 1409 640
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2117 928
Size: 971 218 4932
Fragments arranged by size:
4932 971 218
BbvCI CC'TCA_GC
Cuts at: 2325 2325
Size: 6121
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3387 3421 4312 4346 1903
Size: 34 1450 34 891 34 3678
Fragments arranged by size:
3678 1450 891 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4013 5223 4013
Size: 1210 4911
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6121
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4732 136
Size: 107 122 71 631 605 3060 1525
Fragments arranged by size:
3060 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6116 6116
Size: 6121
BlpI GC'TnA_GC
Cuts at: 3538 3538
Size: 6121
Bme1580I G_kGCm'C
Cuts at: 1790 4182 5338 1790
Size: 2392 1156 2573
Fragments arranged by size:
2573 2392 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6121
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3270 3852 4810
Size: 416 53 83 399 24 1853 582 958
Cuts at: 4810 442
Size: 1753
Fragments arranged by size:
1853 1753 958 582 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2895 4781 1239
Size: 1656 1886 2579
Fragments arranged by size:
2579 1886 1656
Bpu10I CC'TnA_GC
Cuts at: 2325 2325
Size: 6121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4246 5111 5409 4246
Size: 865 298 4958
Fragments arranged by size:
4958 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2088 3007 4784 882
Size: 617 589 919 1777 2219
Fragments arranged by size:
2219 1777 919 617 589
BsaAI yAC'GTr
Cuts at: 493 1204 2280 3912 493
Size: 711 1076 1632 2702
Fragments arranged by size:
2702 1632 1076 711
BsaBI GATnn'nnATC
Cuts at: 6115 6115
Size: 6121
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3928 4310 4945 275
Size: 53 83 186 936 2395 382 635 1451
Fragments arranged by size:
2395 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2016 4554 4952 5226 5373 5784 2016
Size: 2538 398 274 147 411 2353
Fragments arranged by size:
2538 2353 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5896
Fragments arranged by size:
5896 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6121
BseYI C'CCAG_C
Cuts at: 2036 5324 5820 2036
Size: 3288 496 2337
Fragments arranged by size:
3288 2337 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2926 1163
Size: 573 1190 4358
Fragments arranged by size:
4358 1190 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3571 4332 5360 1540
Size: 275 186 1570 761 1028 2301
Fragments arranged by size:
2301 1570 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4182 4267 5338 4182
Size: 85 1071 4965
Fragments arranged by size:
4965 1071 85
BsmI GAATG_Cn'
Cuts at: 3632 3725 3632
Size: 93 6028
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 3440 4182 4267 5338 1391
Size: 399 241 1409 742 85 1071 2174
Fragments arranged by size:
2174 1409 1071 742 399 241 85
BspEI T'CCGG_A
Cuts at: 5784 5784
Size: 6121
BspHI T'CATG_A
Cuts at: 4010 5740 4010
Size: 1730 4391
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2106 2811 3157 3476 844
Size: 1262 705 346 319 3489
Fragments arranged by size:
3489 1262 705 346 319
BsrBI CCG'CTC
Cuts at: 1394 1823 4008 1394
Size: 429 2185 3507
Fragments arranged by size:
3507 2185 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4616 4790 65
Size: 1183 3368 174 1396
Fragments arranged by size:
3368 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4765 4952 1700
Size: 110 2955 187 2869
Fragments arranged by size:
2955 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2969 3021 96
Size: 2873 52 3196
Fragments arranged by size:
3196 2873 52
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6121
BssSI C'ACGA_G
Cuts at: 4181 5193 4181
Size: 1012 5109
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6121
BstBI TT'CG_AA
Cuts at: 2370 4925 2370
Size: 2555 3566
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6121
BstYI r'GATC_y
Cuts at: 1972 2202 2554 3451 3818 4203 4220 5672 5758
Size: 230 352 897 367 385 17 1452 86
Cuts at: 5758 5770 6116 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 897 385 367 352 346 230
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4630
Fragments arranged by size:
4630 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2017 487
Size: 683 56 791 4591
Fragments arranged by size:
4591 791 683 56
BtsI GCAGTG_nn'
Cuts at: 2728 3637 4431 4451 2728
Size: 909 794 20 4398
Fragments arranged by size:
4398 909 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6121
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6086
DraI TTT'AAA
Cuts at: 2158 3425 2158
Size: 1267 4854
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2336 5128 809
Size: 507 1020 2792 1802
Fragments arranged by size:
2792 1802 1020 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3568 4457 6086
Size: 54 1425 50 275 18 1738 889 1629
Cuts at: 6086 8
Size: 43
Fragments arranged by size:
1738 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3568 1537
Size: 275 1756 4090
Fragments arranged by size:
4090 1756 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3215 4051 1353
Size: 1862 836 3423
Fragments arranged by size:
3423 1862 836
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4708 5082 5228 387
Size: 1064 138 3119 374 146 1280
Fragments arranged by size:
3119 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2895 3239 4184 4781 5568 5836 1239
Size: 393 1263 344 945 597 787 268 1524
Fragments arranged by size:
1524 1263 945 787 597 393 344 268
EcoO109I rG'GnC_Cy
Cuts at: 2270 2270
Size: 6121
EcoRI G'AATT_C
Cuts at: 3430 3808 3430
Size: 378 5743
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2289 2289
Size: 6121
FspI TGC'GCA
Cuts at: 4627 4627
Size: 6121
HaeII r_GCGC'y
Cuts at: 1678 5268 1678
Size: 3590 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2744 2776 4762 4794 4836
Size: 32 135 32 1172 32 1986 32 42
Cuts at: 4836 4868 1373
Size: 32 2626
Fragments arranged by size:
2626 1986 1172 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3172 3465 3711 669
Size: 988 281 1234 293 246 3079
Fragments arranged by size:
3079 1234 988 293 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6121
HpaI GTT'AAC
Cuts at: 3711 3711
Size: 6121
KpnI G_GTAC'C
Cuts at: 3446 3446
Size: 6121
MfeI C'AATT_G
Cuts at: 2148 3720 2148
Size: 1572 4549
MluI A'CGCG_T
Cuts at: 5997 5997
Size: 6121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2071 2368 2871 5235 5419 1305
Size: 766 297 503 2364 184 2007
Fragments arranged by size:
2364 2007 766 503 297 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4299
Fragments arranged by size:
4299 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4079 4438 4597 518
Size: 648 2913 359 159 2042
Fragments arranged by size:
2913 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2435 3537 4214 5362 5607 1606
Size: 829 1102 677 1148 245 2120
Fragments arranged by size:
2120 1148 1102 829 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4630
Fragments arranged by size:
4630 939 552
NdeI CA'TA_TG
Cuts at: 387 2977 387
Size: 2590 3531
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6121
NotI GC'GGCC_GC
Cuts at: 3568 3568
Size: 6121
NspI r_CATG'y
Cuts at: 1132 1229 3292 3402 3479 5024 1132
Size: 97 2063 110 77 1545 2229
Fragments arranged by size:
2229 2063 1545 110 97 77
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6121
PciI A'CATG_T
Cuts at: 3288 5020 3288
Size: 1732 4389
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6121
PmeI GTTT'AAAC
Cuts at: 3425 3425
Size: 6121
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4190 4222 5703 5735 4190
Size: 32 1481 32 4576
Fragments arranged by size:
4576 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2270 2270
Size: 6121
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6121
PsiI TTA'TAA
Cuts at: 2640 3691 2640
Size: 1051 5070
PstI C_TGCA'G
Cuts at: 830 2352 2629 3168 3473 830
Size: 1522 277 539 305 3478
Fragments arranged by size:
3478 1522 539 305 277
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6121
PvuII CAG'CTG
Cuts at: 2435 2435
Size: 6121
SalI G'TCGA_C
Cuts at: 1936 3463 1936
Size: 1527 4594
SbfI CC_TGCA'GG
Cuts at: 3473 3473
Size: 6121
ScaI AGT'ACT
Cuts at: 4369 6081 4369
Size: 1712 4409
SfcI C'TryA_G
Cuts at: 826 1045 2348 2548 2625 3164 3469 4604 5285
Size: 219 1303 200 77 539 305 1135 681
Cuts at: 5285 5476 826
Size: 191 1471
Fragments arranged by size:
1471 1303 1135 681 539 305 219 200
191 77
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6121
SmaI CCC'GGG
Cuts at: 1872 3448 1872
Size: 1576 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4225 5126 5388 820
Size: 197 926 2282 901 262 1553
Fragments arranged by size:
2282 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2280 3912 493
Size: 1787 1632 2702
Fragments arranged by size:
2702 1787 1632
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6121
SphI G_CATG'C
Cuts at: 1132 3402 3479 1132
Size: 2270 77 3774
Fragments arranged by size:
3774 2270 77
SspI AAT'ATT
Cuts at: 5 52 4045 5
Size: 47 3993 2081
Fragments arranged by size:
3993 2081 47
StuI AGG'CCT
Cuts at: 1517 2900 1517
Size: 1383 4738
StyI C'CwwG_G
Cuts at: 513 1065 2004 2301 2895 3325 3560 513
Size: 552 939 297 594 430 235 3074
Fragments arranged by size:
3074 939 594 552 430 297 235
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4148 4165 4318 4503 4948 325
Size: 1255 2568 17 153 185 445 1498
Fragments arranged by size:
2568 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2969 3021 4367
Size: 274 80 33 51 1443 992 52 1346
Cuts at: 4367 6079 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1346 992 274 138 80 52
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2856 2888 3256 3288 6081 6113 2856
Size: 32 368 32 2793 32 2864
Fragments arranged by size:
2864 2793 368 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6121
XbaI T'CTAG_A
Cuts at: 3457 3457
Size: 6121
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6121
XmaI C'CCGG_G
Cuts at: 1870 3446 1870
Size: 1576 4545
XmnI GAAnn'nnTTC
Cuts at: 3198 4250 3198
Size: 1052 5069
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3929 276
Size: 53 83 186 936 2395 2468
Fragments arranged by size:
2468 2395 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI