Restriction map for
pFN21AE1344
(Circular) (Six-base) MAPSORT of: pFN21AE1344.seq Check: 4416 from: 1 to: 6385
pFN21AE1344 6385 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3484 3484
Size: 6385
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4195 278
Size: 53 83 186 936 2659 2468
Fragments arranged by size:
2659 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3728 1937
Size: 1791 4594
Acc65I G'GTAC_C
Cuts at: 3706 3706
Size: 6385
AclI AA'CG_TT
Cuts at: 1563 4512 4885 1563
Size: 2949 373 3063
Fragments arranged by size:
3063 2949 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2355 2444 3365 4448 5832 6100 1632
Size: 723 89 921 1083 1384 268 1917
Fragments arranged by size:
1917 1384 1083 921 723 268 89
AflII C'TTAA_G
Cuts at: 820 1017 2276 2638 820
Size: 197 1259 362 4567
Fragments arranged by size:
4567 1259 362 197
AflIII A'CryG_T
Cuts at: 1203 5284 6261 1203
Size: 4081 977 1327
Fragments arranged by size:
4081 1327 977
AgeI A'CCGG_T
Cuts at: 5216 5216
Size: 6385
AhdI GACnn_n'nnGTC
Cuts at: 5114 5114
Size: 6385
AleI CACnn'nnGTG
Cuts at: 1166 2574 1166
Size: 1408 4977
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6385
AlwNI CAG_nnn'CTG
Cuts at: 3106 4128 5700 3106
Size: 1022 1572 3791
Fragments arranged by size:
3791 1572 1022
ApaI G_GGCC'C
Cuts at: 2834 2834
Size: 6385
ApaLI G'TGCA_C
Cuts at: 4442 5598 4442
Size: 1156 5229
ApoI r'AATT_y
Cuts at: 1493 2401 2554 2979 3694 3920 4072 6181 1493
Size: 908 153 425 715 226 152 2109 1697
Fragments arranged by size:
2109 1697 908 715 425 226 153 152
AseI AT'TA_AT
Cuts at: 160 1032 4939 160
Size: 872 3907 1606
Fragments arranged by size:
3907 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6385
AvaI C'yCGr_G
Cuts at: 1870 1943 2064 3710 1870
Size: 73 121 1646 4545
Fragments arranged by size:
4545 1646 121 73
AvrII C'CTAG_G
Cuts at: 2987 2987
Size: 6385
BamHI G'GATC_C
Cuts at: 3715 4082 3715
Size: 367 6018
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3706 5161 618
Size: 325 217 627 1919 1455 1842
Fragments arranged by size:
1919 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2834 3540 3704 1391
Size: 1443 706 164 4072
Fragments arranged by size:
4072 1443 706 164
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5414
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4576 4610 1903
Size: 34 50 34 2555 34 3678
Fragments arranged by size:
3678 2555 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2779 4277 5487 2779
Size: 1498 1210 3677
Fragments arranged by size:
3677 1498 1210
BclI T'GATC_A
Cuts at: 1548 3417 1548
Size: 1869 4516
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4996 136
Size: 107 122 71 631 605 3324 1525
Fragments arranged by size:
3324 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3390 6380 3390
Size: 2990 3395
BlpI GC'TnA_GC
Cuts at: 2921 3802 2921
Size: 881 5504
Bme1580I G_kGCm'C
Cuts at: 1790 2834 3136 4446 5602 1790
Size: 1044 302 1310 1156 2573
Fragments arranged by size:
2573 1310 1156 1044 302
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6385
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4116 5074 442
Size: 416 53 83 399 24 2699 958 1753
Fragments arranged by size:
2699 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6385
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2074 5045 1239
Size: 835 2971 2579
Fragments arranged by size:
2971 2579 835
Bpu10I CC'TnA_GC
Cuts at: 2745 2745
Size: 6385
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2581 2645 2731 3367 4510 5375 5673 2581
Size: 64 86 636 1143 865 298 3293
Fragments arranged by size:
3293 1143 865 636 298 86 64
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5048 882
Size: 617 3549 2219
Fragments arranged by size:
3549 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4176 493
Size: 711 2972 2702
Fragments arranged by size:
2972 2702 711
BsaBI GATnn'nnATC
Cuts at: 6379 6379
Size: 6385
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4192 4574 5209 275
Size: 53 83 186 936 2659 382 635 1451
Fragments arranged by size:
2659 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4818 5216 5490 5637 6048 4818
Size: 398 274 147 411 5155
Fragments arranged by size:
5155 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2122 2152 1178
Size: 30 165 30 719 30 5411
Fragments arranged by size:
5411 719 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2130 1186
Size: 944 5441
BseYI C'CCAG_C
Cuts at: 2734 2780 5588 6084 2734
Size: 46 2808 496 3035
Fragments arranged by size:
3035 2808 496 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5812
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3835 4596 5624 1540
Size: 275 186 1834 761 1028 2301
Fragments arranged by size:
2301 1834 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2614 3364 4446 4531 5602 2614
Size: 750 1082 85 1071 3397
Fragments arranged by size:
3397 1082 1071 750 85
BsmI GAATG_Cn'
Cuts at: 3896 3989 3896
Size: 93 6292
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2614 2834 3136 3364 3540 3704 4446
Size: 399 824 220 302 228 176 164 742
Cuts at: 4446 4531 5602 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 824 742 399 302 228 220
176 164 85
BspEI T'CCGG_A
Cuts at: 6048 6048
Size: 6385
BspHI T'CATG_A
Cuts at: 4274 6004 4274
Size: 1730 4655
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3484 3740 844
Size: 2640 256 3489
Fragments arranged by size:
3489 2640 256
BsrBI CCG'CTC
Cuts at: 1394 1823 4272 1394
Size: 429 2449 3507
Fragments arranged by size:
3507 2449 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4880 5054 65
Size: 1183 3632 174 1396
Fragments arranged by size:
3632 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5029 5216 1700
Size: 110 3219 187 2869
Fragments arranged by size:
3219 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2895 96
Size: 2799 3586
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6385
BssSI C'ACGA_G
Cuts at: 2142 4445 5457 2142
Size: 2303 1012 3070
Fragments arranged by size:
3070 2303 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6385
BstBI TT'CG_AA
Cuts at: 5189 5189
Size: 6385
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6385
BstYI r'GATC_y
Cuts at: 1972 2965 3390 3715 4082 4467 4484 5936 6022
Size: 993 425 325 367 385 17 1452 86
Cuts at: 6022 6034 6380 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 993 425 385 367 346 325
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2915 513
Size: 552 1850 3983
Fragments arranged by size:
3983 1850 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2199 487
Size: 683 56 973 4673
Fragments arranged by size:
4673 973 683 56
BtsI GCAGTG_nn'
Cuts at: 3901 4695 4715 3901
Size: 794 20 5571
Fragments arranged by size:
5571 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6385
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3006 3041 529
Size: 35 2442 35 3873
Fragments arranged by size:
3873 2442 35 35
DraI TTT'AAA
Cuts at: 2683 3689 2683
Size: 1006 5379
DraIII CAC_nnn'GTG
Cuts at: 2938 2938
Size: 6385
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2317 5392 809
Size: 507 1001 3075 1802
Fragments arranged by size:
3075 1802 1001 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2912 3832 4721
Size: 54 1425 50 275 18 1082 920 889
Cuts at: 4721 6350 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1082 920 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3832 1537
Size: 275 2020 4090
Fragments arranged by size:
4090 2020 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2757 3384 3609 4315 1353
Size: 1404 627 225 706 3423
Fragments arranged by size:
3423 1404 706 627 225
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4972 5346 5492 387
Size: 1064 138 3383 374 146 1280
Fragments arranged by size:
3383 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2074 2355 2444 3365 4448 5045 5832
Size: 393 442 281 89 921 1083 597 787
Cuts at: 5832 6100 1239
Size: 268 1524
Fragments arranged by size:
1524 1083 921 787 597 442 393 281
268 89
EcoNI CCTnn'n_nnAGG
Cuts at: 2663 2663
Size: 6385
EcoO109I rG'GnC_Cy
Cuts at: 2830 2831 2830
Size: 1 6384
EcoRI G'AATT_C
Cuts at: 2979 3694 4072 2979
Size: 715 378 5292
Fragments arranged by size:
5292 715 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3095 3095
Size: 6385
FspI TGC'GCA
Cuts at: 4891 4891
Size: 6385
HaeII r_GCGC'y
Cuts at: 1678 5532 1678
Size: 3854 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3277 3309 3509 3541 5026
Size: 32 135 32 1705 32 200 32 1485
Cuts at: 5026 5058 5100 5132 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1705 1485 200 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3729 3975 669
Size: 988 281 1791 246 3079
Fragments arranged by size:
3079 1791 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3648 748
Size: 2900 3485
HpaI GTT'AAC
Cuts at: 3975 3975
Size: 6385
KpnI G_GTAC'C
Cuts at: 3710 3710
Size: 6385
MfeI C'AATT_G
Cuts at: 3984 3984
Size: 6385
MluI A'CGCG_T
Cuts at: 6261 6261
Size: 6385
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5499 5683 1305
Size: 4194 184 2007
Fragments arranged by size:
4194 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2914 10
Size: 54 1425 343 1082 3481
Fragments arranged by size:
3481 1425 1082 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2574 2840 4343 4702 4861 518
Size: 648 1408 266 1503 359 159 2042
Fragments arranged by size:
2042 1503 1408 648 359 266 159
MspA1I CmG'CkG
Cuts at: 1606 2308 2504 2780 3022 3801 4478 5626 5871
Size: 702 196 276 242 779 677 1148 245
Cuts at: 5871 1606
Size: 2120
Fragments arranged by size:
2120 1148 779 702 677 276 245 242
196
NcoI C'CATG_G
Cuts at: 513 1065 2915 513
Size: 552 1850 3983
Fragments arranged by size:
3983 1850 552
NdeI CA'TA_TG
Cuts at: 387 2814 387
Size: 2427 3958
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6385
NotI GC'GGCC_GC
Cuts at: 3832 3832
Size: 6385
NspI r_CATG'y
Cuts at: 1132 1229 2442 2707 3635 3743 5288 1132
Size: 97 1213 265 928 108 1545 2229
Fragments arranged by size:
2229 1545 1213 928 265 108 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6385
PciI A'CATG_T
Cuts at: 5284 5284
Size: 6385
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6385
PfoI T'CCnGG_A
Cuts at: 2614 2614
Size: 6385
PmeI GTTT'AAAC
Cuts at: 3689 3689
Size: 6385
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3087 3119 4454 4486 5967 5999 3087
Size: 32 1335 32 1481 32 3473
Fragments arranged by size:
3473 1481 1335 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6385
PsiI TTA'TAA
Cuts at: 3955 3955
Size: 6385
PspOMI G'GGCC_C
Cuts at: 2830 2830
Size: 6385
PstI C_TGCA'G
Cuts at: 830 2824 3121 3737 830
Size: 1994 297 616 3478
Fragments arranged by size:
3478 1994 616 297
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6385
PvuII CAG'CTG
Cuts at: 2308 2504 2780 3022 2308
Size: 196 276 242 5671
Fragments arranged by size:
5671 276 242 196
SalI G'TCGA_C
Cuts at: 1936 3727 1936
Size: 1791 4594
SbfI CC_TGCA'GG
Cuts at: 3737 3737
Size: 6385
ScaI AGT'ACT
Cuts at: 4633 6345 4633
Size: 1712 4673
SfcI C'TryA_G
Cuts at: 826 1045 2820 3117 3469 3733 4868 5549 5740
Size: 219 1775 297 352 264 1135 681 191
Cuts at: 5740 826
Size: 1471
Fragments arranged by size:
1775 1471 1135 681 352 297 264 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6385
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6385
SmaI CCC'GGG
Cuts at: 1872 3712 1872
Size: 1840 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2276 2560 2638 2660 2710 3346
Size: 197 926 333 284 78 22 50 636
Cuts at: 3346 4489 5390 5652 820
Size: 1143 901 262 1553
Fragments arranged by size:
1553 1143 926 901 636 333 284 262
197 78 50 22
SnaBI TAC'GTA
Cuts at: 493 4176 493
Size: 3683 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6385
SphI G_CATG'C
Cuts at: 1132 3743 1132
Size: 2611 3774
SspI AAT'ATT
Cuts at: 5 52 4309 5
Size: 47 4257 2081
Fragments arranged by size:
4257 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6385
StyI C'CwwG_G
Cuts at: 513 1065 2915 2987 3824 513
Size: 552 1850 72 837 3074
Fragments arranged by size:
3074 1850 837 552 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4412 4429 4582 4767 5212 325
Size: 1255 2832 17 153 185 445 1498
Fragments arranged by size:
2832 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2895 4631 6343
Size: 274 80 33 51 1443 918 1736 1712
Cuts at: 6343 96
Size: 138
Fragments arranged by size:
1736 1712 1443 918 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6345 6377 6345
Size: 32 6353
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6385
XbaI T'CTAG_A
Cuts at: 3092 3721 3092
Size: 629 5756
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2176 2842 2176
Size: 666 5719
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6385
XmaI C'CCGG_G
Cuts at: 1870 3710 1870
Size: 1840 4545
XmnI GAAnn'nnTTC
Cuts at: 4514 4514
Size: 6385
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4193 276
Size: 53 83 186 936 2659 2468
Fragments arranged by size:
2659 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI