Restriction map for
pFN21AE1343
(Circular) (Six-base) MAPSORT of: pFN21AE1343.seq Check: 8388 from: 1 to: 5488
pFN21AE1343 5488 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3298 278
Size: 53 83 186 936 1762 2468
Fragments arranged by size:
2468 1762 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2831 1937
Size: 894 4594
Acc65I G'GTAC_C
Cuts at: 2656 2809 2656
Size: 153 5335
AclI AA'CG_TT
Cuts at: 1563 3615 3988 1563
Size: 2052 373 3063
Fragments arranged by size:
3063 2052 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2109 2412 2426 3551 4935 5203 1632
Size: 477 303 14 1125 1384 268 1917
Fragments arranged by size:
1917 1384 1125 477 303 268 14
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5291
AflIII A'CryG_T
Cuts at: 1203 4387 5364 1203
Size: 3184 977 1327
Fragments arranged by size:
3184 1327 977
AgeI A'CCGG_T
Cuts at: 4319 4319
Size: 5488
AhdI GACnn_n'nnGTC
Cuts at: 4217 4217
Size: 5488
AleI CACnn'nnGTG
Cuts at: 1166 2056 2237 1166
Size: 890 181 4417
Fragments arranged by size:
4417 890 181
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5488
AlwNI CAG_nnn'CTG
Cuts at: 3231 4803 3231
Size: 1572 3916
ApaLI G'TGCA_C
Cuts at: 3545 4701 3545
Size: 1156 4332
ApoI r'AATT_y
Cuts at: 1493 2255 2797 3023 3175 5284 1493
Size: 762 542 226 152 2109 1697
Fragments arranged by size:
2109 1697 762 542 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4042 160
Size: 872 3010 1606
Fragments arranged by size:
3010 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5488
AvaI C'yCGr_G
Cuts at: 1870 1943 2235 2813 1870
Size: 73 292 578 4545
Fragments arranged by size:
4545 578 292 73
BamHI G'GATC_C
Cuts at: 2818 3185 2818
Size: 367 5121
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2239 2656 2809 4264 618
Size: 325 217 627 452 417 153 1455 1842
Fragments arranged by size:
1842 1455 627 452 417 325 217 153
BanII G_rGCy'C
Cuts at: 1391 2272 2807 1391
Size: 881 535 4072
Fragments arranged by size:
4072 881 535
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4517
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3679 3713 1903
Size: 34 1742 34 3678
Fragments arranged by size:
3678 1742 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3380 4590 3380
Size: 1210 4278
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5488
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4099 136
Size: 107 122 71 631 605 2427 1525
Fragments arranged by size:
2427 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2680 5483 2680
Size: 2803 2685
BlpI GC'TnA_GC
Cuts at: 2905 2905
Size: 5488
Bme1580I G_kGCm'C
Cuts at: 1790 3549 4705 1790
Size: 1759 1156 2573
Fragments arranged by size:
2573 1759 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5488
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2163 2275 3219
Size: 416 53 83 399 24 746 112 944
Cuts at: 3219 4177 442
Size: 958 1753
Fragments arranged by size:
1753 958 944 746 416 399 112 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5488
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2435 2708 4148 1239
Size: 1196 273 1440 2579
Fragments arranged by size:
2579 1440 1196 273
Bpu10I CC'TnA_GC
Cuts at: 2271 2779 2271
Size: 508 4980
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3613 4478 4776 3613
Size: 865 298 4325
Fragments arranged by size:
4325 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4151 882
Size: 617 2652 2219
Fragments arranged by size:
2652 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3279 493
Size: 711 2075 2702
Fragments arranged by size:
2702 2075 711
BsaBI GATnn'nnATC
Cuts at: 5482 5482
Size: 5488
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3295 3677 4312 275
Size: 53 83 186 936 1762 382 635 1451
Fragments arranged by size:
1762 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2766 3921 4319 4593 4740 5151 2766
Size: 1155 398 274 147 411 3103
Fragments arranged by size:
3103 1155 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2155 2185 2588 2618 1178
Size: 30 165 30 752 30 403 30 4048
Fragments arranged by size:
4048 752 403 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5488
BseYI C'CCAG_C
Cuts at: 2728 4691 5187 2728
Size: 1963 496 3029
Fragments arranged by size:
3029 1963 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2123 1163
Size: 573 387 4528
Fragments arranged by size:
4528 573 387
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2938 3699 4727 1540
Size: 275 186 937 761 1028 2301
Fragments arranged by size:
2301 1028 937 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3549 3634 4705 3549
Size: 85 1071 4332
Fragments arranged by size:
4332 1071 85
BsmI GAATG_Cn'
Cuts at: 2999 3092 2999
Size: 93 5395
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2272 2807 3549 3634 4705 1391
Size: 399 482 535 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 535 482 399 85
BspEI T'CCGG_A
Cuts at: 2766 5151 2766
Size: 2385 3103
BspHI T'CATG_A
Cuts at: 2011 3377 5107 2011
Size: 1366 1730 2392
Fragments arranged by size:
2392 1730 1366
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2127 2843 844
Size: 1283 716 3489
Fragments arranged by size:
3489 1283 716
BsrBI CCG'CTC
Cuts at: 1394 1823 3375 1394
Size: 429 1552 3507
Fragments arranged by size:
3507 1552 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3983 4157 65
Size: 1183 2735 174 1396
Fragments arranged by size:
2735 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4132 4319 1700
Size: 110 2322 187 2869
Fragments arranged by size:
2869 2322 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5488
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5488
BssSI C'ACGA_G
Cuts at: 3548 4560 3548
Size: 1012 4476
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5488
BstBI TT'CG_AA
Cuts at: 4292 4292
Size: 5488
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5488
BstXI CCAn_nnnn'nTGG
Cuts at: 2058 2437 2058
Size: 379 5109
BstYI r'GATC_y
Cuts at: 1972 2680 2818 3185 3570 3587 5039 5125 5137
Size: 708 138 367 385 17 1452 86 12
Cuts at: 5137 5483 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 708 385 367 346 138 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3997
Fragments arranged by size:
3997 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4749
Fragments arranged by size:
4749 683 56
BtsI GCAGTG_nn'
Cuts at: 2669 3004 3798 3818 2669
Size: 335 794 20 4339
Fragments arranged by size:
4339 794 335 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5488
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5453
DraI TTT'AAA
Cuts at: 2792 2792
Size: 5488
DraIII CAC_nnn'GTG
Cuts at: 2146 2146
Size: 5488
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4495 809
Size: 507 3179 1802
Fragments arranged by size:
3179 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2108 2935 3824
Size: 54 1425 50 275 18 278 827 889
Cuts at: 3824 5453 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 827 278 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2935 1537
Size: 275 1123 4090
Fragments arranged by size:
4090 1123 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2465 3418 1353
Size: 1112 953 3423
Fragments arranged by size:
3423 1112 953
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4075 4449 4595 387
Size: 1064 138 2486 374 146 1280
Fragments arranged by size:
2486 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2109 2412 2426 2435 2708 3551 4148
Size: 393 477 303 14 9 273 843 597
Cuts at: 4148 4935 5203 1239
Size: 787 268 1524
Fragments arranged by size:
1524 843 787 597 477 393 303 273
268 14 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2508 2778 2508
Size: 270 5218
EcoRI G'AATT_C
Cuts at: 2797 3175 2797
Size: 378 5110
FspI TGC'GCA
Cuts at: 3994 3994
Size: 5488
HaeII r_GCGC'y
Cuts at: 1678 4635 1678
Size: 2957 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2588 2620 4129 4161 4203
Size: 32 135 32 1016 32 1509 32 42
Cuts at: 4203 4235 1373
Size: 32 2626
Fragments arranged by size:
2626 1509 1016 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2832 3078 669
Size: 988 281 894 246 3079
Fragments arranged by size:
3079 988 894 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5488
HpaI GTT'AAC
Cuts at: 3078 3078
Size: 5488
KpnI G_GTAC'C
Cuts at: 2660 2813 2660
Size: 153 5335
MfeI C'AATT_G
Cuts at: 2643 3087 2643
Size: 444 5044
MluI A'CGCG_T
Cuts at: 5364 5364
Size: 5488
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4602 4786 1305
Size: 3297 184 2007
Fragments arranged by size:
3297 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2110 10
Size: 54 1425 343 278 3388
Fragments arranged by size:
3388 1425 343 278 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2056 2237 2738 3446 3805 3964 518
Size: 648 890 181 501 708 359 159 2042
Fragments arranged by size:
2042 890 708 648 501 359 181 159
MspA1I CmG'CkG
Cuts at: 1606 2537 2904 3581 4729 4974 1606
Size: 931 367 677 1148 245 2120
Fragments arranged by size:
2120 1148 931 677 367 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3997
Fragments arranged by size:
3997 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5488
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5488
NotI GC'GGCC_GC
Cuts at: 2935 2935
Size: 5488
NspI r_CATG'y
Cuts at: 1132 1229 2846 4391 1132
Size: 97 1617 1545 2229
Fragments arranged by size:
2229 1617 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5488
PciI A'CATG_T
Cuts at: 4387 4387
Size: 5488
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5488
PmeI GTTT'AAAC
Cuts at: 2792 2792
Size: 5488
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3557 3589 5070 5102 3557
Size: 32 1481 32 3943
Fragments arranged by size:
3943 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5488
PsiI TTA'TAA
Cuts at: 3058 3058
Size: 5488
PstI C_TGCA'G
Cuts at: 830 2840 830
Size: 2010 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5488
PvuII CAG'CTG
Cuts at: 2537 2537
Size: 5488
SalI G'TCGA_C
Cuts at: 1936 2830 1936
Size: 894 4594
SbfI CC_TGCA'GG
Cuts at: 2840 2840
Size: 5488
ScaI AGT'ACT
Cuts at: 3736 5448 3736
Size: 1712 3776
SfcI C'TryA_G
Cuts at: 826 1045 2039 2836 3971 4652 4843 826
Size: 219 994 797 1135 681 191 1471
Fragments arranged by size:
1471 1135 994 797 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5488
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5488
SmaI CCC'GGG
Cuts at: 1872 2815 1872
Size: 943 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3592 4493 4755 820
Size: 197 926 1649 901 262 1553
Fragments arranged by size:
1649 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3279 493
Size: 2786 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5488
SphI G_CATG'C
Cuts at: 1132 2846 1132
Size: 1714 3774
SspI AAT'ATT
Cuts at: 5 52 3412 5
Size: 47 3360 2081
Fragments arranged by size:
3360 2081 47
StuI AGG'CCT
Cuts at: 1517 2189 2561 1517
Size: 672 372 4444
Fragments arranged by size:
4444 672 372
StyI C'CwwG_G
Cuts at: 513 1065 2004 2023 2927 513
Size: 552 939 19 904 3074
Fragments arranged by size:
3074 939 904 552 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2482 2711 3515 3532 3685 3870 4315
Size: 1255 902 229 804 17 153 185 445
Cuts at: 4315 325
Size: 1498
Fragments arranged by size:
1498 1255 902 804 445 229 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2148 3734 5446
Size: 274 80 33 51 1443 171 1586 1712
Cuts at: 5446 96
Size: 138
Fragments arranged by size:
1712 1586 1443 274 171 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2155 2187 5448 5480 2155
Size: 32 3261 32 2163
Fragments arranged by size:
3261 2163 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5488
XbaI T'CTAG_A
Cuts at: 2824 2824
Size: 5488
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5488
XmaI C'CCGG_G
Cuts at: 1870 2813 1870
Size: 943 4545
XmnI GAAnn'nnTTC
Cuts at: 3617 3617
Size: 5488
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3296 276
Size: 53 83 186 936 1762 2468
Fragments arranged by size:
2468 1762 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI