Restriction map for
pFN21AE1340
(Circular) (Six-base) MAPSORT of: pFN21AE1340.seq Check: 6036 from: 1 to: 7111
pFN21AE1340 7111 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2236 2681 3008 4334 2236
Size: 445 327 1326 5013
Fragments arranged by size:
5013 1326 445 327
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4921 278
Size: 53 83 186 936 3385 2468
Fragments arranged by size:
3385 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3104 4454 1937
Size: 1167 1350 4594
Fragments arranged by size:
4594 1350 1167
Acc65I G'GTAC_C
Cuts at: 4432 4432
Size: 7111
AclI AA'CG_TT
Cuts at: 1563 5238 5611 1563
Size: 3675 373 3063
Fragments arranged by size:
3675 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2876 4333 5174 6558 6826 1632
Size: 1244 1457 841 1384 268 1917
Fragments arranged by size:
1917 1457 1384 1244 841 268
AflII C'TTAA_G
Cuts at: 820 1017 2558 820
Size: 197 1541 5373
Fragments arranged by size:
5373 1541 197
AflIII A'CryG_T
Cuts at: 1203 2970 3123 4017 6010 6987 1203
Size: 1767 153 894 1993 977 1327
Fragments arranged by size:
1993 1767 1327 977 894 153
AgeI A'CCGG_T
Cuts at: 5942 5942
Size: 7111
AhdI GACnn_n'nnGTC
Cuts at: 2395 4021 5840 2395
Size: 1626 1819 3666
Fragments arranged by size:
3666 1819 1626
AleI CACnn'nnGTG
Cuts at: 1166 2786 4011 1166
Size: 1620 1225 4266
Fragments arranged by size:
4266 1620 1225
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2624 1184
Size: 1440 5671
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4082 4114 4082
Size: 32 7079
AlwNI CAG_nnn'CTG
Cuts at: 2137 2492 2854 3811 4326 4854 6426 2137
Size: 355 362 957 515 528 1572 2822
Fragments arranged by size:
2822 1572 957 528 515 362 355
ApaI G_GGCC'C
Cuts at: 3639 3697 3639
Size: 58 7053
ApaLI G'TGCA_C
Cuts at: 5168 6324 5168
Size: 1156 5955
ApoI r'AATT_y
Cuts at: 1493 4420 4646 4798 6907 1493
Size: 2927 226 152 2109 1697
Fragments arranged by size:
2927 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5665 160
Size: 872 4633 1606
Fragments arranged by size:
4633 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7111
AvaI C'yCGr_G
Cuts at: 1870 1943 3092 3393 3795 3977 4058 4436 1870
Size: 73 1149 301 402 182 81 378 4545
Fragments arranged by size:
4545 1149 402 378 301 182 81 73
AvrII C'CTAG_G
Cuts at: 3421 3421
Size: 7111
BamHI G'GATC_C
Cuts at: 4032 4373 4441 4808 4032
Size: 341 68 367 6335
Fragments arranged by size:
6335 367 341 68
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2224 2647 3198 3699 3973
Size: 325 217 627 437 423 551 501 274
Cuts at: 3973 4432 5887 618
Size: 459 1455 1842
Fragments arranged by size:
1842 1455 627 551 501 459 437 423
325 274 217
BanII G_rGCy'C
Cuts at: 1391 2100 2769 3134 3639 3697 4067 4430 1391
Size: 709 669 365 505 58 370 363 4072
Fragments arranged by size:
4072 709 669 505 370 365 363 58
BbeI G_GCGC'C
Cuts at: 3703 3977 3703
Size: 274 6837
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4339 928
Size: 971 2440 3700
Fragments arranged by size:
3700 2440 971
BbvCI CC'TCA_GC
Cuts at: 3719 3719
Size: 7111
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2866 2900 3001 3035 5302
Size: 34 50 34 845 34 101 34 2267
Cuts at: 5302 5336 1903
Size: 34 3678
Fragments arranged by size:
3678 2267 845 101 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3839 4083 5003 6213 3839
Size: 244 920 1210 4737
Fragments arranged by size:
4737 1210 920 244
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7111
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3009 3708 5722
Size: 107 122 71 631 605 1337 699 2014
Cuts at: 5722 136
Size: 1525
Fragments arranged by size:
2014 1525 1337 699 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3361 4047 7106 3361
Size: 686 3059 3366
Fragments arranged by size:
3366 3059 686
BlpI GC'TnA_GC
Cuts at: 2184 4528 2184
Size: 2344 4767
Bme1580I G_kGCm'C
Cuts at: 1790 2652 3639 3697 5172 6328 1790
Size: 862 987 58 1475 1156 2573
Fragments arranged by size:
2573 1475 1156 987 862 58
BmgBI CAC'GTC
Cuts at: 1318 2803 4018 1318
Size: 1485 1215 4411
Fragments arranged by size:
4411 1485 1215
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3758 4842 5800
Size: 416 53 83 399 24 2341 1084 958
Cuts at: 5800 442
Size: 1753
Fragments arranged by size:
2341 1753 1084 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7111
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3289 4117 3289
Size: 828 6283
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2040 2090 2138 2269 2356 2644 2750 3284
Size: 801 50 48 131 87 288 106 534
Cuts at: 3284 3307 3833 4318 5771 1239
Size: 23 526 485 1453 2579
Fragments arranged by size:
2579 1453 801 534 526 485 288 131
106 87 50 48 23
Bpu10I CC'TnA_GC
Cuts at: 3719 3719
Size: 7111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2114 5236 6101 6399 2114
Size: 3122 865 298 2826
Fragments arranged by size:
3122 2826 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3562 3778 5774 882
Size: 617 2063 216 1996 2219
Fragments arranged by size:
2219 2063 1996 617 216
BsaAI yAC'GTr
Cuts at: 493 1204 3452 3646 4902 493
Size: 711 2248 194 1256 2702
Fragments arranged by size:
2702 2248 1256 711 194
BsaBI GATnn'nnATC
Cuts at: 7105 7105
Size: 7111
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3700 3912 3974 4314
Size: 53 83 186 936 2167 212 62 340
Cuts at: 4314 4918 5300 5935 275
Size: 604 382 635 1451
Fragments arranged by size:
2167 1451 936 635 604 382 340 212
186 83 62 53
BsaWI w'CCGG_w
Cuts at: 5544 5942 6216 6363 6774 5544
Size: 398 274 147 411 5881
Fragments arranged by size:
5881 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2798 2828 2942 2972 4082
Size: 30 165 30 1395 30 114 30 1110
Cuts at: 4082 4112 1178
Size: 30 4177
Fragments arranged by size:
4177 1395 1110 165 114 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3077 3310 4106 4274 4321 1186
Size: 1891 233 796 168 47 3976
Fragments arranged by size:
3976 1891 796 233 168 47
BseYI C'CCAG_C
Cuts at: 2111 2433 2460 2835 6314 6810 2111
Size: 322 27 375 3479 496 2412
Fragments arranged by size:
3479 2412 496 375 322 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3242 3474 3607 4424 1163
Size: 573 1506 232 133 817 3850
Fragments arranged by size:
3850 1506 817 573 232 133
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3196 3589 3952 4561 5322 6350
Size: 275 186 1195 393 363 609 761 1028
Cuts at: 6350 1540
Size: 2301
Fragments arranged by size:
2301 1195 1028 761 609 393 363 275
186
BsiHKAI G_wGCw'C
Cuts at: 3153 5172 5257 6328 3153
Size: 2019 85 1071 3936
Fragments arranged by size:
3936 2019 1071 85
BsmI GAATG_Cn'
Cuts at: 2601 4622 4715 2601
Size: 2021 93 4997
Fragments arranged by size:
4997 2021 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3320 4258 3320
Size: 938 6173
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2652 2769 3134 3153 3639 3697
Size: 399 310 552 117 365 19 486 58
Cuts at: 3697 4067 4430 5172 5257 6328 1391
Size: 370 363 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 552 486 399 370 365
363 310 117 85 58 19
BspEI T'CCGG_A
Cuts at: 6774 6774
Size: 7111
BspHI T'CATG_A
Cuts at: 5000 6730 5000
Size: 1730 5381
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2236 2500 2681 2724 2970 3008 3618 3684
Size: 1392 264 181 43 246 38 610 66
Cuts at: 3684 4334 4466 844
Size: 650 132 3489
Fragments arranged by size:
3489 1392 650 610 264 246 181 132
66 43 38
BsrBI CCG'CTC
Cuts at: 1394 1823 2734 3754 4998 1394
Size: 429 911 1020 1244 3507
Fragments arranged by size:
3507 1244 1020 911 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3859 5606 5780 65
Size: 1183 2611 1747 174 1396
Fragments arranged by size:
2611 1747 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2745 3195 3344 5755 5942 1700
Size: 110 935 450 149 2411 187 2869
Fragments arranged by size:
2869 2411 935 450 187 149 110
BsrGI T'GTAC_A
Cuts at: 96 2823 3999 96
Size: 2727 1176 3208
Fragments arranged by size:
3208 2727 1176
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7111
BssSI C'ACGA_G
Cuts at: 2313 2630 3350 5171 6183 2313
Size: 317 720 1821 1012 3241
Fragments arranged by size:
3241 1821 1012 720 317
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2161 2492 4326 794
Size: 1367 331 1834 3579
Fragments arranged by size:
3579 1834 1367 331
BstBI TT'CG_AA
Cuts at: 5915 5915
Size: 7111
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7111
BstXI CCAn_nnnn'nTGG
Cuts at: 2788 3502 2788
Size: 714 6397
BstYI r'GATC_y
Cuts at: 1972 3361 4032 4047 4373 4441 4808 5193 5210
Size: 1389 671 15 326 68 367 385 17
Cuts at: 5210 6662 6748 6760 7106 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1389 671 385 367 346 326
86 68 17 15 12
BstZ17I GTA'TAC
Cuts at: 3105 3105
Size: 7111
Bsu36I CC'TnA_GG
Cuts at: 2283 2283
Size: 7111
BtgI C'CryG_G
Cuts at: 513 1065 2659 2787 3190 3907 513
Size: 552 1594 128 403 717 3717
Fragments arranged by size:
3717 1594 717 552 403 128
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2844 487
Size: 683 56 1618 4754
Fragments arranged by size:
4754 1618 683 56
BtsI GCAGTG_nn'
Cuts at: 3748 4141 4627 5421 5441 3748
Size: 393 486 794 20 5418
Fragments arranged by size:
5418 794 486 393 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7111
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7076
DraI TTT'AAA
Cuts at: 4415 4415
Size: 7111
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6118 809
Size: 507 4802 1802
Fragments arranged by size:
4802 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2194 2233 2293
Size: 54 1425 50 275 18 364 39 60
Cuts at: 2293 2772 3162 3193 3439 3504 3546 3586 3949
Size: 479 390 31 246 65 42 40 363
Cuts at: 3949 4248 4558 5447 7076 8
Size: 299 310 889 1629 43
Fragments arranged by size:
1629 1425 889 479 390 364 363 310
299 275 246 65 60 54 50 43
42 40 39 31 18
EagI C'GGCC_G
Cuts at: 1537 1812 3193 3586 3949 4558 1537
Size: 275 1381 393 363 609 4090
Fragments arranged by size:
4090 1381 609 393 363 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3280 5041 1353
Size: 1927 1761 3423
Fragments arranged by size:
3423 1927 1761
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4265 5698 6072 6218 387
Size: 1064 138 2676 1433 374 146 1280
Fragments arranged by size:
2676 1433 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2040 2090 2138 2269 2356 2644 2750
Size: 393 408 50 48 131 87 288 106
Cuts at: 2750 2876 3284 3307 3833 4318 4333 5174 5771
Size: 126 408 23 526 485 15 841 597
Cuts at: 5771 6558 6826 1239
Size: 787 268 1524
Fragments arranged by size:
1524 841 787 597 526 485 408 408
393 288 268 131 126 106 87 50
48 23 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2282 2416 3425 4198 2282
Size: 134 1009 773 5195
Fragments arranged by size:
5195 1009 773 134
EcoO109I rG'GnC_Cy
Cuts at: 2032 2389 2407 2953 3067 3541 3635 3636 3710
Size: 357 18 546 114 474 94 1 74
Cuts at: 3710 3838 4240 2032
Size: 128 402 4903
Fragments arranged by size:
4903 546 474 402 357 128 114 94
74 18 1
EcoRI G'AATT_C
Cuts at: 4420 4798 4420
Size: 378 6733
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2108 2108
Size: 7111
FspI TGC'GCA
Cuts at: 5617 5617
Size: 7111
HaeII r_GCGC'y
Cuts at: 1678 3092 3188 3703 3977 4193 4232 6258 1678
Size: 1414 96 515 274 216 39 2026 2531
Fragments arranged by size:
2531 2026 1414 515 274 216 96 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2381 2413 3346 3378 3515
Size: 32 135 32 809 32 933 32 137
Cuts at: 3515 3542 3547 3574 4007 4039 5752 5784 5826
Size: 27 5 27 433 32 1713 32 42
Cuts at: 5826 5858 1373
Size: 32 2626
Fragments arranged by size:
2626 1713 933 809 433 137 135 42
32 32 32 32 32 32 32 27
27 5
HincII GTy'rAC
Cuts at: 669 1657 1938 4455 4701 669
Size: 988 281 2517 246 3079
Fragments arranged by size:
3079 2517 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7111
HpaI GTT'AAC
Cuts at: 4701 4701
Size: 7111
KasI G'GCGC_C
Cuts at: 3699 3973 3699
Size: 274 6837
KpnI G_GTAC'C
Cuts at: 4436 4436
Size: 7111
MfeI C'AATT_G
Cuts at: 4710 4710
Size: 7111
MluI A'CGCG_T
Cuts at: 3123 6987 3123
Size: 3864 3247
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6225 6409 1305
Size: 4920 184 2007
Fragments arranged by size:
4920 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2196 3441 10
Size: 54 1425 343 364 1245 3680
Fragments arranged by size:
3680 1425 1245 364 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2786 4011 4085 5069 5428 5587 518
Size: 648 1620 1225 74 984 359 159 2042
Fragments arranged by size:
2042 1620 1225 984 648 359 159 74
MspA1I CmG'CkG
Cuts at: 1606 2517 2525 2553 2661 3327 3909 4219 4527
Size: 911 8 28 108 666 582 310 308
Cuts at: 4527 5204 6352 6597 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 911 677 666 582 310 308
245 108 28 8
NaeI GCC'GGC
Cuts at: 3197 3346 3197
Size: 149 6962
NarI GG'CG_CC
Cuts at: 3700 3974 3700
Size: 274 6837
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6559
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7111
NgoMIV G'CCGG_C
Cuts at: 3195 3344 3195
Size: 149 6962
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7111
NotI GC'GGCC_GC
Cuts at: 4558 4558
Size: 7111
NspI r_CATG'y
Cuts at: 1132 1229 2486 3805 4469 6014 1132
Size: 97 1257 1319 664 1545 2229
Fragments arranged by size:
2229 1545 1319 1257 664 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7111
PciI A'CATG_T
Cuts at: 6010 6010
Size: 7111
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2467 2553 1108
Size: 1359 86 5666
Fragments arranged by size:
5666 1359 86
PfoI T'CCnGG_A
Cuts at: 3818 3818
Size: 7111
PmeI GTTT'AAAC
Cuts at: 4415 4415
Size: 7111
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4082 4114 5180 5212 6693 6725 4082
Size: 32 1066 32 1481 32 4468
Fragments arranged by size:
4468 1481 1066 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2032 2389 2407 2953 3067 3541 3838 4240 2032
Size: 357 18 546 114 474 297 402 4903
Fragments arranged by size:
4903 546 474 402 357 297 114 18
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7111
PsiI TTA'TAA
Cuts at: 4681 4681
Size: 7111
PspOMI G'GGCC_C
Cuts at: 3635 3693 3635
Size: 58 7053
PstI C_TGCA'G
Cuts at: 830 2337 2530 2760 2929 4463 830
Size: 1507 193 230 169 1534 3478
Fragments arranged by size:
3478 1534 1507 230 193 169
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7111
PvuII CAG'CTG
Cuts at: 2525 4219 2525
Size: 1694 5417
RsrII CG'GwC_CG
Cuts at: 2656 2656
Size: 7111
SacII CC_GC'GG
Cuts at: 2662 3910 2662
Size: 1248 5863
SalI G'TCGA_C
Cuts at: 1936 4453 1936
Size: 2517 4594
SanDI GG'GwC_CC
Cuts at: 2407 2407
Size: 7111
SapI GCTCTTCn'nnn_
Cuts at: 3280 3280
Size: 7111
SbfI CC_TGCA'GG
Cuts at: 2760 4463 2760
Size: 1703 5408
ScaI AGT'ACT
Cuts at: 5359 7071 5359
Size: 1712 5399
SexAI A'CCwGG_T
Cuts at: 3552 3786 3552
Size: 234 6877
SfcI C'TryA_G
Cuts at: 826 1045 2333 2526 2756 2925 4459 5594 6275
Size: 219 1288 193 230 169 1534 1135 681
Cuts at: 6275 6466 826
Size: 191 1471
Fragments arranged by size:
1534 1471 1288 1135 681 230 219 193
191 169
SfoI GGC'GCC
Cuts at: 3701 3975 3701
Size: 274 6837
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7111
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7111
SmaI CCC'GGG
Cuts at: 1872 3395 4438 1872
Size: 1523 1043 4545
Fragments arranged by size:
4545 1523 1043
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2093 2558 5215 6116 6378 820
Size: 197 926 150 465 2657 901 262 1553
Fragments arranged by size:
2657 1553 926 901 465 262 197 150
SnaBI TAC'GTA
Cuts at: 493 4902 493
Size: 4409 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7111
SphI G_CATG'C
Cuts at: 1132 2486 3805 4469 1132
Size: 1354 1319 664 3774
Fragments arranged by size:
3774 1354 1319 664
SspI AAT'ATT
Cuts at: 5 52 5035 5
Size: 47 4983 2081
Fragments arranged by size:
4983 2081 47
StuI AGG'CCT
Cuts at: 1517 2075 2573 3461 3983 4204 1517
Size: 558 498 888 522 221 4424
Fragments arranged by size:
4424 888 558 522 498 221
StyI C'CwwG_G
Cuts at: 513 1065 2149 3141 3421 3666 4550 513
Size: 552 1084 992 280 245 884 3074
Fragments arranged by size:
3074 1084 992 884 552 280 245
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3641 4146 5138 5155 5308 5493 5938
Size: 1255 2061 505 992 17 153 185 445
Cuts at: 5938 325
Size: 1498
Fragments arranged by size:
2061 1498 1255 992 505 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2823 2994 3999
Size: 274 80 33 51 1443 846 171 1005
Cuts at: 3999 5357 7069 96
Size: 1358 1712 138
Fragments arranged by size:
1712 1443 1358 1005 846 274 171 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2798 2830 7071 7103 2798
Size: 32 4241 32 2806
Fragments arranged by size:
4241 2806 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3554 4238 1316
Size: 2238 684 4189
Fragments arranged by size:
4189 2238 684
XbaI T'CTAG_A
Cuts at: 3358 4447 3358
Size: 1089 6022
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2788 2788
Size: 7111
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7111
XmaI C'CCGG_G
Cuts at: 1870 3393 4436 1870
Size: 1523 1043 4545
Fragments arranged by size:
4545 1523 1043
XmnI GAAnn'nnTTC
Cuts at: 5240 5240
Size: 7111
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4919 276
Size: 53 83 186 936 3385 2468
Fragments arranged by size:
3385 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BfrBI BsiWI DraIII EcoICRI EcoRV
FseI FspAI NruI NsiI PacI PmlI PspXI PsrI
SacI SfiI SrfI SwaI