Restriction map for
pFN21AE1336
(Circular) (Six-base) MAPSORT of: pFN21AE1336.seq Check: 1469 from: 1 to: 5455
pFN21AE1336 5455 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2328 2328
Size: 5455
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2061 3265 278
Size: 53 83 186 936 525 1204 2468
Fragments arranged by size:
2468 1204 936 525 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2798 1937
Size: 861 4594
Acc65I G'GTAC_C
Cuts at: 2776 2776
Size: 5455
AclI AA'CG_TT
Cuts at: 1563 3582 3955 1563
Size: 2019 373 3063
Fragments arranged by size:
3063 2019 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2186 3518 4902 5170 1632
Size: 554 1332 1384 268 1917
Fragments arranged by size:
1917 1384 1332 554 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5258
AflIII A'CryG_T
Cuts at: 1203 4354 5331 1203
Size: 3151 977 1327
Fragments arranged by size:
3151 1327 977
AgeI A'CCGG_T
Cuts at: 4286 4286
Size: 5455
AhdI GACnn_n'nnGTC
Cuts at: 4184 4184
Size: 5455
AleI CACnn'nnGTG
Cuts at: 1166 2297 1166
Size: 1131 4324
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5455
AlwNI CAG_nnn'CTG
Cuts at: 3198 4770 3198
Size: 1572 3883
ApaI G_GGCC'C
Cuts at: 2163 2163
Size: 5455
ApaLI G'TGCA_C
Cuts at: 3512 4668 3512
Size: 1156 4299
ApoI r'AATT_y
Cuts at: 1493 2378 2764 2990 3142 5251 1493
Size: 885 386 226 152 2109 1697
Fragments arranged by size:
2109 1697 885 386 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4009 160
Size: 872 2977 1606
Fragments arranged by size:
2977 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5455
AvaI C'yCGr_G
Cuts at: 1870 1943 2780 1870
Size: 73 837 4545
Fragments arranged by size:
4545 837 73
BamHI G'GATC_C
Cuts at: 2785 3152 2785
Size: 367 5088
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2299 2776 4231 618
Size: 325 217 627 512 477 1455 1842
Fragments arranged by size:
1842 1455 627 512 477 325 217
BanII G_rGCy'C
Cuts at: 1391 2163 2253 2774 1391
Size: 772 90 521 4072
Fragments arranged by size:
4072 772 521 90
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4484
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3646 3680 1903
Size: 34 1709 34 3678
Fragments arranged by size:
3678 1709 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3347 4557 3347
Size: 1210 4245
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5455
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4066 136
Size: 107 122 71 631 605 2394 1525
Fragments arranged by size:
2394 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5450 5450
Size: 5455
BlpI GC'TnA_GC
Cuts at: 2872 2872
Size: 5455
Bme1580I G_kGCm'C
Cuts at: 1790 2163 2304 2622 3516 4672 1790
Size: 373 141 318 894 1156 2573
Fragments arranged by size:
2573 1156 894 373 318 141
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5455
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3186 4144 442
Size: 416 53 83 399 24 1769 958 1753
Fragments arranged by size:
1769 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2429 1056
Size: 1373 4082
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2582 4115 1239
Size: 1343 1533 2579
Fragments arranged by size:
2579 1533 1343
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3580 4445 4743 3580
Size: 865 298 4292
Fragments arranged by size:
4292 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4118 882
Size: 617 2619 2219
Fragments arranged by size:
2619 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3246 493
Size: 711 2042 2702
Fragments arranged by size:
2702 2042 711
BsaBI GATnn'nnATC
Cuts at: 5449 5449
Size: 5455
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2058 2081 3262 3644
Size: 53 83 186 936 525 23 1181 382
Cuts at: 3644 4279 275
Size: 635 1451
Fragments arranged by size:
1451 1181 936 635 525 382 186 83
53 23
BsaWI w'CCGG_w
Cuts at: 3888 4286 4560 4707 5118 3888
Size: 398 274 147 411 4225
Fragments arranged by size:
4225 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2336 2366 1178
Size: 30 165 30 933 30 4267
Fragments arranged by size:
4267 933 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2384 1186
Size: 1198 4257
BseYI C'CCAG_C
Cuts at: 2580 2661 4658 5154 2580
Size: 81 1997 496 2881
Fragments arranged by size:
2881 1997 496 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2096 1163
Size: 573 360 4522
Fragments arranged by size:
4522 573 360
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2905 3666 4694 1540
Size: 275 186 904 761 1028 2301
Fragments arranged by size:
2301 1028 904 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2253 3516 3601 4672 2253
Size: 1263 85 1071 3036
Fragments arranged by size:
3036 1263 1071 85
BsmI GAATG_Cn'
Cuts at: 2966 3059 2966
Size: 93 5362
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2163 2253 2304 2622 2774 3516 3601
Size: 399 373 90 51 318 152 742 85
Cuts at: 3601 4672 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 373 318 152 90
85 51
BspEI T'CCGG_A
Cuts at: 5118 5118
Size: 5455
BspHI T'CATG_A
Cuts at: 3344 5074 3344
Size: 1730 3725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2160 2328 2810 844
Size: 1316 168 482 3489
Fragments arranged by size:
3489 1316 482 168
BsrBI CCG'CTC
Cuts at: 1394 1823 3342 1394
Size: 429 1519 3507
Fragments arranged by size:
3507 1519 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2547 3950 4124 65
Size: 1183 1299 1403 174 1396
Fragments arranged by size:
1403 1396 1299 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4099 4286 1700
Size: 110 2289 187 2869
Fragments arranged by size:
2869 2289 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5455
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5455
BssSI C'ACGA_G
Cuts at: 2609 3515 4527 2609
Size: 906 1012 3537
Fragments arranged by size:
3537 1012 906
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2566 794
Size: 1772 3683
BstBI TT'CG_AA
Cuts at: 4259 4259
Size: 5455
BstEII G'GTnAC_C
Cuts at: 1184 2626 1184
Size: 1442 4013
BstYI r'GATC_y
Cuts at: 1972 2280 2785 3152 3537 3554 5006 5092 5104
Size: 308 505 367 385 17 1452 86 12
Cuts at: 5104 5450 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 505 385 367 346 308 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2735 2735
Size: 5455
BtgI C'CryG_G
Cuts at: 513 1065 2064 2551 513
Size: 552 999 487 3417
Fragments arranged by size:
3417 999 552 487
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4716
Fragments arranged by size:
4716 683 56
BtsI GCAGTG_nn'
Cuts at: 2971 3765 3785 2971
Size: 794 20 4641
Fragments arranged by size:
4641 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5455
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5420
DraI TTT'AAA
Cuts at: 2759 2759
Size: 5455
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4462 809
Size: 507 3146 1802
Fragments arranged by size:
3146 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2902 3791 5420
Size: 54 1425 50 275 18 1072 889 1629
Cuts at: 5420 8
Size: 43
Fragments arranged by size:
1629 1425 1072 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2902 1537
Size: 275 1090 4090
Fragments arranged by size:
4090 1090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2258 2361 2448 3385 1353
Size: 905 103 87 937 3423
Fragments arranged by size:
3423 937 905 103 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4042 4416 4562 387
Size: 1064 138 2453 374 146 1280
Fragments arranged by size:
2453 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2251 2251
Size: 5455
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2186 2582 3518 4115 4902 5170 1239
Size: 393 554 396 936 597 787 268 1524
Fragments arranged by size:
1524 936 787 597 554 396 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2159 2160 2159
Size: 1 5454
EcoRI G'AATT_C
Cuts at: 2764 3142 2764
Size: 378 5077
FspI TGC'GCA
Cuts at: 3961 3961
Size: 5455
HaeII r_GCGC'y
Cuts at: 1678 4602 1678
Size: 2924 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2128 2160 2578 2610 4096
Size: 32 135 32 556 32 418 32 1486
Cuts at: 4096 4128 4170 4202 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1486 556 418 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2128 2799 3045 669
Size: 988 281 190 671 246 3079
Fragments arranged by size:
3079 988 671 281 246 190
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5455
HpaI GTT'AAC
Cuts at: 2128 3045 2128
Size: 917 4538
KpnI G_GTAC'C
Cuts at: 2780 2780
Size: 5455
MfeI C'AATT_G
Cuts at: 3054 3054
Size: 5455
MluI A'CGCG_T
Cuts at: 5331 5331
Size: 5455
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2385 4569 4753 1305
Size: 1080 2184 184 2007
Fragments arranged by size:
2184 2007 1080 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3633
Fragments arranged by size:
3633 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2297 2528 2613 3413 3772 3931 518
Size: 648 1131 231 85 800 359 159 2042
Fragments arranged by size:
2042 1131 800 648 359 231 159 85
MspA1I CmG'CkG
Cuts at: 1606 2041 2553 2584 2871 3548 4696 4941 1606
Size: 435 512 31 287 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 512 435 287 245 31
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4903
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5455
NheI G'CTAG_C
Cuts at: 1052 2425 1052
Size: 1373 4082
NotI GC'GGCC_GC
Cuts at: 2902 2902
Size: 5455
NspI r_CATG'y
Cuts at: 1132 1229 2560 2813 4358 1132
Size: 97 1331 253 1545 2229
Fragments arranged by size:
2229 1545 1331 253 97
PasI CC'CwG_GG
Cuts at: 1348 2507 2622 1348
Size: 1159 115 4181
Fragments arranged by size:
4181 1159 115
PciI A'CATG_T
Cuts at: 4354 4354
Size: 5455
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5455
PfoI T'CCnGG_A
Cuts at: 2244 2244
Size: 5455
PmeI GTTT'AAAC
Cuts at: 2759 2759
Size: 5455
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3524 3556 5037 5069 3524
Size: 32 1481 32 3910
Fragments arranged by size:
3910 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2441 1613
Size: 828 4627
PsiI TTA'TAA
Cuts at: 3025 3025
Size: 5455
PspOMI G'GGCC_C
Cuts at: 2159 2159
Size: 5455
PstI C_TGCA'G
Cuts at: 830 2496 2807 830
Size: 1666 311 3478
Fragments arranged by size:
3478 1666 311
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5455
SacI G_AGCT'C
Cuts at: 2253 2253
Size: 5455
SacII CC_GC'GG
Cuts at: 2554 2554
Size: 5455
SalI G'TCGA_C
Cuts at: 1936 2797 1936
Size: 861 4594
SapI GCTCTTCn'nnn_
Cuts at: 2258 2258
Size: 5455
SbfI CC_TGCA'GG
Cuts at: 2807 2807
Size: 5455
ScaI AGT'ACT
Cuts at: 3703 5415 3703
Size: 1712 3743
SfcI C'TryA_G
Cuts at: 826 1045 2492 2803 3938 4619 4810 826
Size: 219 1447 311 1135 681 191 1471
Fragments arranged by size:
1471 1447 1135 681 311 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5455
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5455
SmaI CCC'GGG
Cuts at: 1872 2782 1872
Size: 910 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3559 4460 4722 820
Size: 197 926 1616 901 262 1553
Fragments arranged by size:
1616 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3246 493
Size: 2753 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5455
SphI G_CATG'C
Cuts at: 1132 2560 2813 1132
Size: 1428 253 3774
Fragments arranged by size:
3774 1428 253
SspI AAT'ATT
Cuts at: 5 52 3379 5
Size: 47 3327 2081
Fragments arranged by size:
3327 2081 47
StuI AGG'CCT
Cuts at: 1517 2396 1517
Size: 879 4576
StyI C'CwwG_G
Cuts at: 513 1065 2646 2894 513
Size: 552 1581 248 3074
Fragments arranged by size:
3074 1581 552 248
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3482 3499 3652 3837 4282 325
Size: 1255 1902 17 153 185 445 1498
Fragments arranged by size:
1902 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3701 5413 96
Size: 274 80 33 51 1443 1724 1712 138
Fragments arranged by size:
1724 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2336 2368 5415 5447 2336
Size: 32 3047 32 2344
Fragments arranged by size:
3047 2344 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2011 1316
Size: 695 4760
XbaI T'CTAG_A
Cuts at: 2791 2791
Size: 5455
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2446 2446
Size: 5455
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5455
XmaI C'CCGG_G
Cuts at: 1870 2780 1870
Size: 910 4545
XmnI GAAnn'nnTTC
Cuts at: 3584 3584
Size: 5455
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2059 3263 276
Size: 53 83 186 936 525 1204 2468
Fragments arranged by size:
2468 1204 936 525 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI SacI SacII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII
RsrII SanDI SexAI SfiI SfoI SrfI SwaI