Restriction map for
pFN21AE1333
(Circular) (Six-base) MAPSORT of: pFN21AE1333.seq Check: 1446 from: 1 to: 6496
pFN21AE1333 6496 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2252 2252
Size: 6496
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4306 278
Size: 53 83 186 936 2770 2468
Fragments arranged by size:
2770 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3839 1937
Size: 1902 4594
Acc65I G'GTAC_C
Cuts at: 2991 3597 3817 2991
Size: 606 220 5670
Fragments arranged by size:
5670 606 220
AclI AA'CG_TT
Cuts at: 1563 4623 4996 1563
Size: 3060 373 3063
Fragments arranged by size:
3063 3060 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4559 5943 6211 1632
Size: 2927 1384 268 1917
Fragments arranged by size:
2927 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6299
AflIII A'CryG_T
Cuts at: 1203 5395 6372 1203
Size: 4192 977 1327
Fragments arranged by size:
4192 1327 977
AgeI A'CCGG_T
Cuts at: 5327 5327
Size: 6496
AhdI GACnn_n'nnGTC
Cuts at: 5225 5225
Size: 6496
AleI CACnn'nnGTG
Cuts at: 1166 2565 2792 1166
Size: 1399 227 4870
Fragments arranged by size:
4870 1399 227
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6496
AlwNI CAG_nnn'CTG
Cuts at: 3594 4239 5811 3594
Size: 645 1572 4279
Fragments arranged by size:
4279 1572 645
ApaLI G'TGCA_C
Cuts at: 4553 5709 4553
Size: 1156 5340
ApoI r'AATT_y
Cuts at: 1493 2414 2575 3055 3805 4031 4183 6292 1493
Size: 921 161 480 750 226 152 2109 1697
Fragments arranged by size:
2109 1697 921 750 480 226 161 152
AseI AT'TA_AT
Cuts at: 160 1032 2904 5050 160
Size: 872 1872 2146 1606
Fragments arranged by size:
2146 1872 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6496
AvaI C'yCGr_G
Cuts at: 1870 1943 3821 1870
Size: 73 1878 4545
Fragments arranged by size:
4545 1878 73
AvrII C'CTAG_G
Cuts at: 2945 2945
Size: 6496
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2983 3016 2983
Size: 33 6463
BamHI G'GATC_C
Cuts at: 3826 4193 3826
Size: 367 6129
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2991 3597 3607 3817 5272
Size: 325 217 627 1204 606 10 210 1455
Cuts at: 5272 618
Size: 1842
Fragments arranged by size:
1842 1455 1204 627 606 325 217 210
10
BanII G_rGCy'C
Cuts at: 1391 2035 2892 3815 1391
Size: 644 857 923 4072
Fragments arranged by size:
4072 923 857 644
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5525
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4687 4721 1903
Size: 34 50 34 2666 34 3678
Fragments arranged by size:
3678 2666 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4388 5598 4388
Size: 1210 5286
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6496
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2253 5107 136
Size: 107 122 71 631 605 581 2854 1525
Fragments arranged by size:
2854 1525 631 605 581 122 107 71
BglII A'GATC_T
Cuts at: 6491 6491
Size: 6496
BlpI GC'TnA_GC
Cuts at: 3913 3913
Size: 6496
Bme1580I G_kGCm'C
Cuts at: 1790 3612 4557 5713 1790
Size: 1822 945 1156 2573
Fragments arranged by size:
2573 1822 1156 945
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6496
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4227 5185 442
Size: 416 53 83 399 24 2810 958 1753
Fragments arranged by size:
2810 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6496
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2517 2517
Size: 6496
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2056 2512 2523 3414 3731 5156 1239
Size: 817 456 11 891 317 1425 2579
Fragments arranged by size:
2579 1425 891 817 456 317 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4621 5486 5784 4621
Size: 865 298 5333
Fragments arranged by size:
5333 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2680 2758 3200 3620 5159 882
Size: 617 1181 78 442 420 1539 2219
Fragments arranged by size:
2219 1539 1181 617 442 420 78
BsaAI yAC'GTr
Cuts at: 493 1204 2363 3246 4287 493
Size: 711 1159 883 1041 2702
Fragments arranged by size:
2702 1159 1041 883 711
BsaBI GATnn'nnATC
Cuts at: 3498 6490 3498
Size: 2992 3504
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4303 4685 5320 275
Size: 53 83 186 936 2770 382 635 1451
Fragments arranged by size:
2770 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4929 5327 5601 5748 6159 4929
Size: 398 274 147 411 5266
Fragments arranged by size:
5266 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6271
Fragments arranged by size:
6271 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2496 2526 2865 1186
Size: 1310 30 339 4817
Fragments arranged by size:
4817 1310 339 30
BseYI C'CCAG_C
Cuts at: 5699 6195 5699
Size: 496 6000
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5923
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3946 4707 5735 1540
Size: 275 186 1945 761 1028 2301
Fragments arranged by size:
2301 1945 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3654 4557 4642 5713 3654
Size: 903 85 1071 4437
Fragments arranged by size:
4437 1071 903 85
BsmI GAATG_Cn'
Cuts at: 4007 4100 4007
Size: 93 6403
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2035 2892 3612 3654 3815 4557 4642
Size: 399 245 857 720 42 161 742 85
Cuts at: 4642 5713 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 857 742 720 399 245 161
85 42
BspEI T'CCGG_A
Cuts at: 6159 6159
Size: 6496
BspHI T'CATG_A
Cuts at: 3202 4385 6115 3202
Size: 1183 1730 3583
Fragments arranged by size:
3583 1730 1183
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2252 3851 844
Size: 1408 1599 3489
Fragments arranged by size:
3489 1599 1408
BsrBI CCG'CTC
Cuts at: 1394 1823 4383 1394
Size: 429 2560 3507
Fragments arranged by size:
3507 2560 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4991 5165 65
Size: 1183 3743 174 1396
Fragments arranged by size:
3743 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5140 5327 1700
Size: 110 3330 187 2869
Fragments arranged by size:
3330 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6496
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6496
BssSI C'ACGA_G
Cuts at: 4556 5568 4556
Size: 1012 5484
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6496
BstBI TT'CG_AA
Cuts at: 5300 5300
Size: 6496
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6496
BstXI CCAn_nnnn'nTGG
Cuts at: 3239 3239
Size: 6496
BstYI r'GATC_y
Cuts at: 1972 2264 3826 4193 4578 4595 6047 6133 6145
Size: 292 1562 367 385 17 1452 86 12
Cuts at: 6145 6491 1972
Size: 346 1977
Fragments arranged by size:
1977 1562 1452 385 367 346 292 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3304 513
Size: 552 2239 3705
Fragments arranged by size:
3705 2239 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5757
Fragments arranged by size:
5757 683 56
BtsI GCAGTG_nn'
Cuts at: 2839 3680 4012 4806 4826 2839
Size: 841 332 794 20 4509
Fragments arranged by size:
4509 841 794 332 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6496
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6461
DraI TTT'AAA
Cuts at: 3800 3800
Size: 6496
DraIII CAC_nnn'GTG
Cuts at: 2513 2513
Size: 6496
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5503 809
Size: 507 4187 1802
Fragments arranged by size:
4187 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3943 4832 6461
Size: 54 1425 50 275 18 2113 889 1629
Cuts at: 6461 8
Size: 43
Fragments arranged by size:
2113 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3943 1537
Size: 275 2131 4090
Fragments arranged by size:
4090 2131 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2040 2108 3342 4426 1353
Size: 687 68 1234 1084 3423
Fragments arranged by size:
3423 1234 1084 687 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5083 5457 5603 387
Size: 1064 138 3494 374 146 1280
Fragments arranged by size:
3494 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2056 2512 2523 3414 3731 4559 5156
Size: 393 424 456 11 891 317 828 597
Cuts at: 5156 5943 6211 1239
Size: 787 268 1524
Fragments arranged by size:
1524 891 828 787 597 456 424 393
317 268 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2071 2071
Size: 6496
EcoRI G'AATT_C
Cuts at: 2414 3805 4183 2414
Size: 1391 378 4727
Fragments arranged by size:
4727 1391 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3591 3591
Size: 6496
FspI TGC'GCA
Cuts at: 5002 5002
Size: 6496
HaeII r_GCGC'y
Cuts at: 1678 5643 1678
Size: 3965 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2110 2142 5137 5169 5211
Size: 32 135 32 538 32 2995 32 42
Cuts at: 5211 5243 1373
Size: 32 2626
Fragments arranged by size:
2995 2626 538 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2202 3840 4086 669
Size: 988 281 264 1638 246 3079
Fragments arranged by size:
3079 1638 988 281 264 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6496
HpaI GTT'AAC
Cuts at: 4086 4086
Size: 6496
KpnI G_GTAC'C
Cuts at: 2995 3601 3821 2995
Size: 606 220 5670
Fragments arranged by size:
5670 606 220
MfeI C'AATT_G
Cuts at: 2149 4095 2149
Size: 1946 4550
MluI A'CGCG_T
Cuts at: 6372 6372
Size: 6496
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3367 3437 5610 5794 1305
Size: 2062 70 2173 184 2007
Fragments arranged by size:
2173 2062 2007 184 70
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4674
Fragments arranged by size:
4674 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2565 2792 2847 3196 3265 4454 4813
Size: 648 1399 227 55 349 69 1189 359
Cuts at: 4813 4972 518
Size: 159 2042
Fragments arranged by size:
2042 1399 1189 648 359 349 227 159
69 55
MspA1I CmG'CkG
Cuts at: 1606 2421 2565 2610 2997 3912 4589 5737 5982
Size: 815 144 45 387 915 677 1148 245
Cuts at: 5982 1606
Size: 2120
Fragments arranged by size:
2120 1148 915 815 677 387 245 144
45
NcoI C'CATG_G
Cuts at: 513 1065 3304 513
Size: 552 2239 3705
Fragments arranged by size:
3705 2239 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6496
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6496
NotI GC'GGCC_GC
Cuts at: 3943 3943
Size: 6496
NspI r_CATG'y
Cuts at: 1132 1229 2927 3854 5399 1132
Size: 97 1698 927 1545 2229
Fragments arranged by size:
2229 1698 1545 927 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6496
PciI A'CATG_T
Cuts at: 5395 5395
Size: 6496
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2079 3269 1108
Size: 971 1190 4335
Fragments arranged by size:
4335 1190 971
PmeI GTTT'AAAC
Cuts at: 3800 3800
Size: 6496
PmlI CAC'GTG
Cuts at: 3246 3246
Size: 6496
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3636 3668 4565 4597 6078 6110 3636
Size: 32 897 32 1481 32 4022
Fragments arranged by size:
4022 1481 897 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6496
PsiI TTA'TAA
Cuts at: 3315 4066 3315
Size: 751 5745
PstI C_TGCA'G
Cuts at: 830 3410 3848 830
Size: 2580 438 3478
Fragments arranged by size:
3478 2580 438
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6496
PvuII CAG'CTG
Cuts at: 2421 2610 2421
Size: 189 6307
SalI G'TCGA_C
Cuts at: 1936 3838 1936
Size: 1902 4594
SapI GCTCTTCn'nnn_
Cuts at: 2040 2040
Size: 6496
SbfI CC_TGCA'GG
Cuts at: 3848 3848
Size: 6496
ScaI AGT'ACT
Cuts at: 4744 6456 4744
Size: 1712 4784
SexAI A'CCwGG_T
Cuts at: 3593 3593
Size: 6496
SfcI C'TryA_G
Cuts at: 826 1045 2605 3021 3406 3752 3844 4979 5660
Size: 219 1560 416 385 346 92 1135 681
Cuts at: 5660 5851 826
Size: 191 1471
Fragments arranged by size:
1560 1471 1135 681 416 385 346 219
191 92
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6496
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6496
SmaI CCC'GGG
Cuts at: 1872 3823 1872
Size: 1951 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4600 5501 5763 820
Size: 197 926 2657 901 262 1553
Fragments arranged by size:
2657 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2363 4287 493
Size: 1870 1924 2702
Fragments arranged by size:
2702 1924 1870
SpeI A'CTAG_T
Cuts at: 152 2456 3793 152
Size: 2304 1337 2855
Fragments arranged by size:
2855 2304 1337
SphI G_CATG'C
Cuts at: 1132 3854 1132
Size: 2722 3774
SspI AAT'ATT
Cuts at: 5 52 4420 5
Size: 47 4368 2081
Fragments arranged by size:
4368 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6496
StyI C'CwwG_G
Cuts at: 513 1065 2945 3076 3232 3304 3935 513
Size: 552 1880 131 156 72 631 3074
Fragments arranged by size:
3074 1880 631 552 156 131 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2179 2299 2364 4523 4540 4693 4878
Size: 1255 599 120 65 2159 17 153 185
Cuts at: 4878 5323 325
Size: 445 1498
Fragments arranged by size:
2159 1498 1255 599 445 185 153 120
65 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4742 6454 96
Size: 274 80 33 51 1443 2765 1712 138
Fragments arranged by size:
2765 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3235 3267 6456 6488 3235
Size: 32 3189 32 3243
Fragments arranged by size:
3243 3189 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6496
XbaI T'CTAG_A
Cuts at: 3832 3832
Size: 6496
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2928 3539 2928
Size: 611 5885
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6496
XmaI C'CCGG_G
Cuts at: 1870 3821 1870
Size: 1951 4545
XmnI GAAnn'nnTTC
Cuts at: 4625 4625
Size: 6496
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4304 276
Size: 53 83 186 936 2770 2468
Fragments arranged by size:
2770 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SfiI SfoI SrfI SwaI