Restriction map for
pFN21AE1331
(Circular) (Six-base) MAPSORT of: pFN21AE1331.seq Check: 3515 from: 1 to: 5713
pFN21AE1331 5713 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3523 278
Size: 53 83 186 936 1987 2468
Fragments arranged by size:
2468 1987 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3056 1937
Size: 1119 4594
Acc65I G'GTAC_C
Cuts at: 3034 3034
Size: 5713
AclI AA'CG_TT
Cuts at: 1563 3840 4213 1563
Size: 2277 373 3063
Fragments arranged by size:
3063 2277 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2314 2633 2717 2991 3776 5160 5428 1632
Size: 682 319 84 274 785 1384 268 1917
Fragments arranged by size:
1917 1384 785 682 319 274 268 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5516
AflIII A'CryG_T
Cuts at: 1203 2828 4612 5589 1203
Size: 1625 1784 977 1327
Fragments arranged by size:
1784 1625 1327 977
AgeI A'CCGG_T
Cuts at: 4544 4544
Size: 5713
AhdI GACnn_n'nnGTC
Cuts at: 4442 4442
Size: 5713
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5713
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2357 2966 1184
Size: 1173 609 3931
Fragments arranged by size:
3931 1173 609
AlwNI CAG_nnn'CTG
Cuts at: 2848 3456 5028 2848
Size: 608 1572 3533
Fragments arranged by size:
3533 1572 608
ApaLI G'TGCA_C
Cuts at: 3770 4926 3770
Size: 1156 4557
ApoI r'AATT_y
Cuts at: 1493 2083 2520 3022 3248 3400 5509 1493
Size: 590 437 502 226 152 2109 1697
Fragments arranged by size:
2109 1697 590 502 437 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4267 160
Size: 872 3235 1606
Fragments arranged by size:
3235 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5713
AvaI C'yCGr_G
Cuts at: 1870 1943 3038 1870
Size: 73 1095 4545
Fragments arranged by size:
4545 1095 73
BamHI G'GATC_C
Cuts at: 3043 3410 3043
Size: 367 5346
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2129 2341 2448 3034 4489
Size: 325 217 627 342 212 107 586 1455
Cuts at: 4489 618
Size: 1842
Fragments arranged by size:
1842 1455 627 586 342 325 217 212
107
BanII G_rGCy'C
Cuts at: 1391 2034 2511 3032 1391
Size: 643 477 521 4072
Fragments arranged by size:
4072 643 521 477
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4742
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3904 3938 1903
Size: 34 1967 34 3678
Fragments arranged by size:
3678 1967 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2231 3605 4815 2231
Size: 1374 1210 3129
Fragments arranged by size:
3129 1374 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5713
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2843 4324 136
Size: 107 122 71 631 605 1171 1481 1525
Fragments arranged by size:
1525 1481 1171 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5708 5708
Size: 5713
BlpI GC'TnA_GC
Cuts at: 2419 3130 2419
Size: 711 5002
Bme1580I G_kGCm'C
Cuts at: 1790 2326 2346 3774 4930 1790
Size: 536 20 1428 1156 2573
Fragments arranged by size:
2573 1428 1156 536 20
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5713
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2483 3444 4402
Size: 416 53 83 399 24 1066 961 958
Cuts at: 4402 442
Size: 1753
Fragments arranged by size:
1753 1066 961 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2232 1056
Size: 1176 4537
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4373 1239
Size: 3134 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2038 3838 4703 5001 2038
Size: 1800 865 298 2750
Fragments arranged by size:
2750 1800 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4376 882
Size: 617 2877 2219
Fragments arranged by size:
2877 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3504 493
Size: 711 2300 2702
Fragments arranged by size:
2702 2300 711
BsaBI GATnn'nnATC
Cuts at: 2735 5707 2735
Size: 2972 2741
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3520 3902 4537 275
Size: 53 83 186 936 1987 382 635 1451
Fragments arranged by size:
1987 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4146 4544 4818 4965 5376 4146
Size: 398 274 147 411 4483
Fragments arranged by size:
4483 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5488
Fragments arranged by size:
5488 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2903 1186
Size: 1717 3996
BseYI C'CCAG_C
Cuts at: 2838 4916 5412 2838
Size: 2078 496 3139
Fragments arranged by size:
3139 2078 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2003 2169 2499 2572 1163
Size: 573 267 166 330 73 4304
Fragments arranged by size:
4304 573 330 267 166 73
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3163 3924 4952 1540
Size: 275 186 1162 761 1028 2301
Fragments arranged by size:
2301 1162 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2034 3774 3859 4930 2034
Size: 1740 85 1071 2817
Fragments arranged by size:
2817 1740 1071 85
BsmI GAATG_Cn'
Cuts at: 3224 3317 3224
Size: 93 5620
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2326 2346 2511 3032 3774 3859
Size: 399 244 292 20 165 521 742 85
Cuts at: 3859 4930 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 521 399 292 244 165
85 20
BspEI T'CCGG_A
Cuts at: 5376 5376
Size: 5713
BspHI T'CATG_A
Cuts at: 3602 5332 3602
Size: 1730 3983
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2178 2862 3068 844
Size: 1334 684 206 3489
Fragments arranged by size:
3489 1334 684 206
BsrBI CCG'CTC
Cuts at: 1394 1823 3600 1394
Size: 429 1777 3507
Fragments arranged by size:
3507 1777 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4208 4382 65
Size: 1183 2960 174 1396
Fragments arranged by size:
2960 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4357 4544 1700
Size: 110 2547 187 2869
Fragments arranged by size:
2869 2547 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5713
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5713
BssSI C'ACGA_G
Cuts at: 3773 4785 3773
Size: 1012 4701
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5713
BstBI TT'CG_AA
Cuts at: 2070 4517 2070
Size: 2447 3266
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5713
BstXI CCAn_nnnn'nTGG
Cuts at: 2215 2727 2215
Size: 512 5201
BstYI r'GATC_y
Cuts at: 1972 3043 3410 3795 3812 5264 5350 5362 5708
Size: 1071 367 385 17 1452 86 12 346
Cuts at: 5708 1972
Size: 1977
Fragments arranged by size:
1977 1452 1071 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4222
Fragments arranged by size:
4222 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4974
Fragments arranged by size:
4974 683 56
BtsI GCAGTG_nn'
Cuts at: 2186 2326 2546 3229 4023 4043 2186
Size: 140 220 683 794 20 3856
Fragments arranged by size:
3856 794 683 220 140 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5713
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5678
DraI TTT'AAA
Cuts at: 3017 3017
Size: 5713
DraIII CAC_nnn'GTG
Cuts at: 2896 2896
Size: 5713
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4720 809
Size: 507 3404 1802
Fragments arranged by size:
3404 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2562 3160 4049
Size: 54 1425 50 275 18 732 598 889
Cuts at: 4049 5678 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 732 598 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3160 1537
Size: 275 1348 4090
Fragments arranged by size:
4090 1348 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2249 2270 2333 3643 1353
Size: 896 21 63 1310 3423
Fragments arranged by size:
3423 1310 896 63 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2042 4300 4674 4820 387
Size: 1064 138 453 2258 374 146 1280
Fragments arranged by size:
2258 1280 1064 453 374 146 138
EcoICRI GAG'CTC
Cuts at: 2032 2032
Size: 5713
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2314 2633 2717 2991 3776 4373 5160
Size: 393 682 319 84 274 785 597 787
Cuts at: 5160 5428 1239
Size: 268 1524
Fragments arranged by size:
1524 787 785 682 597 393 319 274
268 84
EcoO109I rG'GnC_Cy
Cuts at: 2488 2723 2488
Size: 235 5478
EcoRI G'AATT_C
Cuts at: 2083 3022 3400 2083
Size: 939 378 4396
Fragments arranged by size:
4396 939 378
FspI TGC'GCA
Cuts at: 4219 4219
Size: 5713
HaeII r_GCGC'y
Cuts at: 1678 4860 1678
Size: 3182 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4354 4386 4428 4460 1373
Size: 32 135 32 2782 32 42 32 2626
Fragments arranged by size:
2782 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2826 3057 3303 669
Size: 988 281 888 231 246 3079
Fragments arranged by size:
3079 988 888 281 246 231
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5713
HpaI GTT'AAC
Cuts at: 3303 3303
Size: 5713
KpnI G_GTAC'C
Cuts at: 3038 3038
Size: 5713
MfeI C'AATT_G
Cuts at: 3312 3312
Size: 5713
MluI A'CGCG_T
Cuts at: 5589 5589
Size: 5713
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4827 5011 1305
Size: 3522 184 2007
Fragments arranged by size:
3522 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2564 10
Size: 54 1425 343 732 3159
Fragments arranged by size:
3159 1425 732 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2339 3671 4030 4189 518
Size: 648 1173 1332 359 159 2042
Fragments arranged by size:
2042 1332 1173 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2062 2147 2271 2308 3129 3806 4954 5199
Size: 456 85 124 37 821 677 1148 245
Cuts at: 5199 1606
Size: 2120
Fragments arranged by size:
2120 1148 821 677 456 245 124 85
37
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4222
Fragments arranged by size:
4222 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5713
NheI G'CTAG_C
Cuts at: 1052 2228 1052
Size: 1176 4537
NotI GC'GGCC_GC
Cuts at: 3160 3160
Size: 5713
NspI r_CATG'y
Cuts at: 1132 1229 2832 3071 4616 1132
Size: 97 1603 239 1545 2229
Fragments arranged by size:
2229 1603 1545 239 97
PasI CC'CwG_GG
Cuts at: 1348 2357 2461 2503 1348
Size: 1009 104 42 4558
Fragments arranged by size:
4558 1009 104 42
PciI A'CATG_T
Cuts at: 2828 4612 2828
Size: 1784 3929
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2473 1108
Size: 1365 4348
PmeI GTTT'AAAC
Cuts at: 3017 3017
Size: 5713
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3782 3814 5295 5327 3782
Size: 32 1481 32 4168
Fragments arranged by size:
4168 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2488 2488
Size: 5713
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5713
PsiI TTA'TAA
Cuts at: 3283 3283
Size: 5713
PstI C_TGCA'G
Cuts at: 830 3065 830
Size: 2235 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5713
PvuII CAG'CTG
Cuts at: 2062 2147 2308 2062
Size: 85 161 5467
Fragments arranged by size:
5467 161 85
SacI G_AGCT'C
Cuts at: 2034 2034
Size: 5713
SalI G'TCGA_C
Cuts at: 1936 3055 1936
Size: 1119 4594
SbfI CC_TGCA'GG
Cuts at: 3065 3065
Size: 5713
ScaI AGT'ACT
Cuts at: 3961 5673 3961
Size: 1712 4001
SfcI C'TryA_G
Cuts at: 826 1045 2637 3061 4196 4877 5068 826
Size: 219 1592 424 1135 681 191 1471
Fragments arranged by size:
1592 1471 1135 681 424 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5713
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5713
SmaI CCC'GGG
Cuts at: 1872 3040 1872
Size: 1168 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2053 3817 4718 4980 820
Size: 197 926 110 1764 901 262 1553
Fragments arranged by size:
1764 1553 926 901 262 197 110
SnaBI TAC'GTA
Cuts at: 493 3504 493
Size: 3011 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5713
SphI G_CATG'C
Cuts at: 1132 3071 1132
Size: 1939 3774
SspI AAT'ATT
Cuts at: 5 52 3637 5
Size: 47 3585 2081
Fragments arranged by size:
3585 2081 47
StuI AGG'CCT
Cuts at: 1517 2496 1517
Size: 979 4734
StyI C'CwwG_G
Cuts at: 513 1065 2004 2688 2998 3152 513
Size: 552 939 684 310 154 3074
Fragments arranged by size:
3074 939 684 552 310 154
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2465 3740 3757 3910 4095 4540 325
Size: 1255 885 1275 17 153 185 445 1498
Fragments arranged by size:
1498 1275 1255 885 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3959 5671 96
Size: 274 80 33 51 1443 1982 1712 138
Fragments arranged by size:
1982 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5673 5705 5673
Size: 32 5681
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5713
XbaI T'CTAG_A
Cuts at: 3049 3049
Size: 5713
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5713
XmaI C'CCGG_G
Cuts at: 1870 3038 1870
Size: 1168 4545
XmnI GAAnn'nnTTC
Cuts at: 3842 3842
Size: 5713
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3521 276
Size: 53 83 186 936 1987 2468
Fragments arranged by size:
2468 1987 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI