Restriction map for
pFN21AE1329
(Circular) (Six-base) MAPSORT of: pFN21AE1329.seq Check: 4075 from: 1 to: 6496
pFN21AE1329 6496 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4306 278
Size: 53 83 186 936 2770 2468
Fragments arranged by size:
2770 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2631 3839 1937
Size: 694 1208 4594
Fragments arranged by size:
4594 1208 694
Acc65I G'GTAC_C
Cuts at: 2340 3817 2340
Size: 1477 5019
AclI AA'CG_TT
Cuts at: 1563 4623 4996 1563
Size: 3060 373 3063
Fragments arranged by size:
3063 3060 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4559 5943 6211 1632
Size: 2927 1384 268 1917
Fragments arranged by size:
2927 1917 1384 268
AfeI AGC'GCT
Cuts at: 3494 3494
Size: 6496
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6299
AflIII A'CryG_T
Cuts at: 1203 2352 5395 6372 1203
Size: 1149 3043 977 1327
Fragments arranged by size:
3043 1327 1149 977
AgeI A'CCGG_T
Cuts at: 5327 5327
Size: 6496
AhdI GACnn_n'nnGTC
Cuts at: 5225 5225
Size: 6496
AleI CACnn'nnGTG
Cuts at: 1166 2660 1166
Size: 1494 5002
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6496
AlwNI CAG_nnn'CTG
Cuts at: 2797 3253 4239 5811 2797
Size: 456 986 1572 3482
Fragments arranged by size:
3482 1572 986 456
ApaI G_GGCC'C
Cuts at: 3138 3354 3138
Size: 216 6280
ApaLI G'TGCA_C
Cuts at: 3362 4553 5709 3362
Size: 1191 1156 4149
Fragments arranged by size:
4149 1191 1156
ApoI r'AATT_y
Cuts at: 1493 3805 4031 4183 6292 1493
Size: 2312 226 152 2109 1697
Fragments arranged by size:
2312 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5050 160
Size: 872 4018 1606
Fragments arranged by size:
4018 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6496
AvaI C'yCGr_G
Cuts at: 1870 1943 2900 3063 3107 3496 3821 1870
Size: 73 957 163 44 389 325 4545
Fragments arranged by size:
4545 957 389 325 163 73 44
BamHI G'GATC_C
Cuts at: 2169 3826 4193 2169
Size: 1657 367 4472
Fragments arranged by size:
4472 1657 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2282 2340 2621 3392 3817
Size: 325 217 627 495 58 281 771 425
Cuts at: 3817 5272 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 771 627 495 425 325 281
217 58
BanII G_rGCy'C
Cuts at: 1391 2400 3138 3354 3815 1391
Size: 1009 738 216 461 4072
Fragments arranged by size:
4072 1009 738 461 216
BbeI G_GCGC'C
Cuts at: 2625 2625
Size: 6496
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2603 3473 928
Size: 971 704 870 3951
Fragments arranged by size:
3951 971 870 704
BbvCI CC'TCA_GC
Cuts at: 2426 3299 2426
Size: 873 5623
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4687 4721 1903
Size: 34 50 34 2666 34 3678
Fragments arranged by size:
3678 2666 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2716 4388 5598 2716
Size: 1672 1210 3614
Fragments arranged by size:
3614 1672 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6496
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3390 5107 136
Size: 107 122 71 631 605 1718 1717 1525
Fragments arranged by size:
1718 1717 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3234 6491 3234
Size: 3257 3239
BlpI GC'TnA_GC
Cuts at: 3913 3913
Size: 6496
Bme1580I G_kGCm'C
Cuts at: 1790 3138 3354 3366 4557 5713 1790
Size: 1348 216 12 1191 1156 2573
Fragments arranged by size:
2573 1348 1191 1156 216 12
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6496
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2097 3273 3544
Size: 416 53 83 399 24 680 1176 271
Cuts at: 3544 4227 5185 442
Size: 683 958 1753
Fragments arranged by size:
1753 1176 958 683 680 416 399 271
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6496
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2632 3445 2632
Size: 813 5683
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2457 3014 3599 5156 1239
Size: 1218 557 585 1557 2579
Fragments arranged by size:
2579 1557 1218 585 557
Bpu10I CC'TnA_GC
Cuts at: 2426 2495 3254 3299 2426
Size: 69 759 45 5623
Fragments arranged by size:
5623 759 69 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2048 2282 3770 4621 5486 5784 2048
Size: 234 1488 851 865 298 2760
Fragments arranged by size:
2760 1488 865 851 298 234
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2025 2048 2549 3205 3213 5159 882
Size: 617 526 23 501 656 8 1946 2219
Fragments arranged by size:
2219 1946 656 617 526 501 23 8
BsaAI yAC'GTr
Cuts at: 493 1204 3538 4287 493
Size: 711 2334 749 2702
Fragments arranged by size:
2702 2334 749 711
BsaBI GATnn'nnATC
Cuts at: 6490 6490
Size: 6496
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2622 4303 4685 5320
Size: 53 83 186 936 1089 1681 382 635
Cuts at: 5320 275
Size: 1451
Fragments arranged by size:
1681 1451 1089 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4929 5327 5601 5748 6159 4929
Size: 398 274 147 411 5266
Fragments arranged by size:
5266 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2430 2460 3506 3536 3599
Size: 30 165 30 1027 30 1046 30 63
Cuts at: 3599 3629 3683 3713 1178
Size: 30 54 30 3961
Fragments arranged by size:
3961 1046 1027 165 63 54 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2624 2762 3746 1186
Size: 1438 138 984 3936
Fragments arranged by size:
3936 1438 984 138
BseYI C'CCAG_C
Cuts at: 3637 5699 6195 3637
Size: 2062 496 3938
Fragments arranged by size:
3938 2062 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3527 3695 1163
Size: 573 1791 168 3964
Fragments arranged by size:
3964 1791 573 168
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3385 3946 4707 5735 1540
Size: 275 186 1384 561 761 1028 2301
Fragments arranged by size:
2301 1384 1028 761 561 275 186
BsiHKAI G_wGCw'C
Cuts at: 2460 3366 4557 4642 5713 2460
Size: 906 1191 85 1071 3243
Fragments arranged by size:
3243 1191 1071 906 85
BsmI GAATG_Cn'
Cuts at: 2671 3655 4007 4100 2671
Size: 984 352 93 5067
Fragments arranged by size:
5067 984 352 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2366 2366
Size: 6496
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2400 2460 3138 3354 3366 3815 4557
Size: 399 610 60 678 216 12 449 742
Cuts at: 4557 4642 5713 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 678 610 449 399 216
85 60 12
BspEI T'CCGG_A
Cuts at: 6159 6159
Size: 6496
BspHI T'CATG_A
Cuts at: 3054 4385 6115 3054
Size: 1331 1730 3435
Fragments arranged by size:
3435 1730 1331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2643 3717 3851 844
Size: 1799 1074 134 3489
Fragments arranged by size:
3489 1799 1074 134
BsrBI CCG'CTC
Cuts at: 1394 1823 2494 4383 1394
Size: 429 671 1889 3507
Fragments arranged by size:
3507 1889 671 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4991 5165 65
Size: 1183 3743 174 1396
Fragments arranged by size:
3743 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3374 5140 5327 1700
Size: 110 1564 1766 187 2869
Fragments arranged by size:
2869 1766 1564 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6496
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6496
BssSI C'ACGA_G
Cuts at: 2656 4556 5568 2656
Size: 1900 1012 3584
Fragments arranged by size:
3584 1900 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6496
BstBI TT'CG_AA
Cuts at: 5300 5300
Size: 6496
BstEII G'GTnAC_C
Cuts at: 1184 2155 2500 1184
Size: 971 345 5180
Fragments arranged by size:
5180 971 345
BstXI CCAn_nnnn'nTGG
Cuts at: 2357 3442 3478 2357
Size: 1085 36 5375
Fragments arranged by size:
5375 1085 36
BstYI r'GATC_y
Cuts at: 1972 2169 3234 3826 4193 4578 4595 6047 6133
Size: 197 1065 592 367 385 17 1452 86
Cuts at: 6133 6145 6491 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1065 592 385 367 346 197
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2617 2617
Size: 6496
BtgI C'CryG_G
Cuts at: 513 1065 2014 2080 2344 3000 3268 513
Size: 552 949 66 264 656 268 3741
Fragments arranged by size:
3741 949 656 552 268 264 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2959 3529 487
Size: 683 56 1733 570 3454
Fragments arranged by size:
3454 1733 683 570 56
BtsI GCAGTG_nn'
Cuts at: 3364 4012 4806 4826 3364
Size: 648 794 20 5034
Fragments arranged by size:
5034 794 648 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6496
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6461
DraI TTT'AAA
Cuts at: 3800 3800
Size: 6496
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3272 5503 809
Size: 507 1956 2231 1802
Fragments arranged by size:
2231 1956 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2347 2810 3382
Size: 54 1425 50 275 18 517 463 572
Cuts at: 3382 3540 3943 4832 6461 8
Size: 158 403 889 1629 43
Fragments arranged by size:
1629 1425 889 572 517 463 403 275
158 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3382 3943 1537
Size: 275 1570 561 4090
Fragments arranged by size:
4090 1570 561 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3764 4426 1353
Size: 2411 662 3423
Fragments arranged by size:
3423 2411 662
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5083 5457 5603 387
Size: 1064 138 3494 374 146 1280
Fragments arranged by size:
3494 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2457 3014 3599 4559 5156 5943 6211
Size: 393 825 557 585 960 597 787 268
Cuts at: 6211 1239
Size: 1524
Fragments arranged by size:
1524 960 825 787 597 585 557 393
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2063 3345 2063
Size: 1282 5214
EcoO109I rG'GnC_Cy
Cuts at: 2948 3135 3274 3350 3439 3583 2948
Size: 187 139 76 89 144 5861
Fragments arranged by size:
5861 187 144 139 89 76
EcoRI G'AATT_C
Cuts at: 3805 4183 3805
Size: 378 6118
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2755 2755
Size: 6496
FspI TGC'GCA
Cuts at: 5002 5002
Size: 6496
HaeII r_GCGC'y
Cuts at: 1678 2625 3375 3496 5643 1678
Size: 947 750 121 2147 2531
Fragments arranged by size:
2531 2147 947 750 121
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5137 5169 5211 5243 1373
Size: 32 135 32 3565 32 42 32 2626
Fragments arranged by size:
3565 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3840 4086 669
Size: 988 281 1902 246 3079
Fragments arranged by size:
3079 1902 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6496
HpaI GTT'AAC
Cuts at: 4086 4086
Size: 6496
KasI G'GCGC_C
Cuts at: 2621 2621
Size: 6496
KpnI G_GTAC'C
Cuts at: 2344 3821 2344
Size: 1477 5019
MfeI C'AATT_G
Cuts at: 4095 4095
Size: 6496
MluI A'CGCG_T
Cuts at: 6372 6372
Size: 6496
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2673 2931 3591 5610 5794 1305
Size: 1368 258 660 2019 184 2007
Fragments arranged by size:
2019 2007 1368 660 258 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2349 3542 10
Size: 54 1425 343 517 1193 2964
Fragments arranged by size:
2964 1425 1193 517 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2660 3053 3359 3410 3416 3476 3536
Size: 648 1494 393 306 51 6 60 60
Cuts at: 3536 4454 4813 4972 518
Size: 918 359 159 2042
Fragments arranged by size:
2042 1494 918 648 393 359 306 159
60 60 51 6
MspA1I CmG'CkG
Cuts at: 1606 2308 2809 2941 3208 3325 3751 3912 4589
Size: 702 501 132 267 117 426 161 677
Cuts at: 4589 5737 5982 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 702 677 501 426 267 245
161 132 117
NaeI GCC'GGC
Cuts at: 3376 3376
Size: 6496
NarI GG'CG_CC
Cuts at: 2622 2622
Size: 6496
NcoI C'CATG_G
Cuts at: 513 1065 2344 3268 513
Size: 552 1279 924 3741
Fragments arranged by size:
3741 1279 924 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6496
NgoMIV G'CCGG_C
Cuts at: 3374 3374
Size: 6496
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6496
NotI GC'GGCC_GC
Cuts at: 3943 3943
Size: 6496
NspI r_CATG'y
Cuts at: 1132 1229 2356 3121 3415 3854 5399 1132
Size: 97 1127 765 294 439 1545 2229
Fragments arranged by size:
2229 1545 1127 765 439 294 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6496
PciI A'CATG_T
Cuts at: 2352 5395 2352
Size: 3043 3453
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2797 1108
Size: 1689 4807
PfoI T'CCnGG_A
Cuts at: 3306 3306
Size: 6496
PmeI GTTT'AAAC
Cuts at: 3800 3800
Size: 6496
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3763 3795 4565 4597 6078 6110 3763
Size: 32 770 32 1481 32 4149
Fragments arranged by size:
4149 1481 770 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3274 3583 3274
Size: 309 6187
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6496
PsiI TTA'TAA
Cuts at: 4066 4066
Size: 6496
PspOMI G'GGCC_C
Cuts at: 3134 3350 3134
Size: 216 6280
PstI C_TGCA'G
Cuts at: 830 2271 2385 2880 3371 3591 3848 830
Size: 1441 114 495 491 220 257 3478
Fragments arranged by size:
3478 1441 495 491 257 220 114
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6496
PvuII CAG'CTG
Cuts at: 2941 2941
Size: 6496
SalI G'TCGA_C
Cuts at: 1936 3838 1936
Size: 1902 4594
SanDI GG'GwC_CC
Cuts at: 3274 3274
Size: 6496
SapI GCTCTTCn'nnn_
Cuts at: 3764 3764
Size: 6496
SbfI CC_TGCA'GG
Cuts at: 2271 2880 3848 2271
Size: 609 968 4919
Fragments arranged by size:
4919 968 609
ScaI AGT'ACT
Cuts at: 2243 4744 6456 2243
Size: 2501 1712 2283
Fragments arranged by size:
2501 2283 1712
SexAI A'CCwGG_T
Cuts at: 2577 2577
Size: 6496
SfcI C'TryA_G
Cuts at: 826 1045 2267 2381 2876 3367 3587 3844 4979
Size: 219 1222 114 495 491 220 257 1135
Cuts at: 4979 5660 5851 826
Size: 681 191 1471
Fragments arranged by size:
1471 1222 1135 681 495 491 257 220
219 191 114
SfoI GGC'GCC
Cuts at: 2623 2623
Size: 6496
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6496
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6496
SmaI CCC'GGG
Cuts at: 1872 2902 3823 1872
Size: 1030 921 4545
Fragments arranged by size:
4545 1030 921
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2063 2297 3785 4600 5501 5763
Size: 197 926 120 234 1488 815 901 262
Cuts at: 5763 820
Size: 1553
Fragments arranged by size:
1553 1488 926 901 815 262 234 197
120
SnaBI TAC'GTA
Cuts at: 493 4287 493
Size: 3794 2702
SpeI A'CTAG_T
Cuts at: 152 2327 152
Size: 2175 4321
SphI G_CATG'C
Cuts at: 1132 3121 3854 1132
Size: 1989 733 3774
Fragments arranged by size:
3774 1989 733
SspI AAT'ATT
Cuts at: 5 52 4420 5
Size: 47 4368 2081
Fragments arranged by size:
4368 2081 47
StuI AGG'CCT
Cuts at: 1517 2615 3665 1517
Size: 1098 1050 4348
Fragments arranged by size:
4348 1098 1050
StyI C'CwwG_G
Cuts at: 513 1065 2344 2388 2472 2730 3268 3435 3660
Size: 552 1279 44 84 258 538 167 225
Cuts at: 3660 3935 513
Size: 275 3074
Fragments arranged by size:
3074 1279 552 538 275 258 225 167
84 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2007 2519 2534 4523 4540 4693 4878
Size: 1255 427 512 15 1989 17 153 185
Cuts at: 4878 5323 325
Size: 445 1498
Fragments arranged by size:
1989 1498 1255 512 445 427 185 153
17 15
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2241 3522 4742
Size: 274 80 33 51 1443 264 1281 1220
Cuts at: 4742 6454 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1281 1220 274 264 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2430 2462 6456 6488 2430
Size: 32 3994 32 2438
Fragments arranged by size:
3994 2438 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6496
XbaI T'CTAG_A
Cuts at: 3832 3832
Size: 6496
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2474 3067 3439 2474
Size: 593 372 5531
Fragments arranged by size:
5531 593 372
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6496
XmaI C'CCGG_G
Cuts at: 1870 2900 3821 1870
Size: 1030 921 4545
Fragments arranged by size:
4545 1030 921
XmnI GAAnn'nnTTC
Cuts at: 4625 4625
Size: 6496
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4304 276
Size: 53 83 186 936 2770 2468
Fragments arranged by size:
2770 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SacII SfiI SrfI
SwaI