Restriction map for
pFN21AE1328
(Circular) (Six-base) MAPSORT of: pFN21AE1328.seq Check: 4306 from: 1 to: 5797
pFN21AE1328 5797 bp
With 182 enzymes: SgfI *
July 14, 2018 22:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2576 2576
Size: 5797
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3607 278
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3140 1937
Size: 1203 4594
Acc65I G'GTAC_C
Cuts at: 2649 3118 2649
Size: 469 5328
AclI AA'CG_TT
Cuts at: 1563 3924 4297 1563
Size: 2361 373 3063
Fragments arranged by size:
3063 2361 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2147 2618 3860 5244 5512 1632
Size: 515 471 1242 1384 268 1917
Fragments arranged by size:
1917 1384 1242 515 471 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5600
AflIII A'CryG_T
Cuts at: 1203 2073 2075 4696 5673 1203
Size: 870 2 2621 977 1327
Fragments arranged by size:
2621 1327 977 870 2
AgeI A'CCGG_T
Cuts at: 4628 4628
Size: 5797
AhdI GACnn_n'nnGTC
Cuts at: 4526 4526
Size: 5797
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5797
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2558 1184
Size: 1374 4423
AlwNI CAG_nnn'CTG
Cuts at: 3540 5112 3540
Size: 1572 4225
ApaLI G'TGCA_C
Cuts at: 2533 3854 5010 2533
Size: 1321 1156 3320
Fragments arranged by size:
3320 1321 1156
ApoI r'AATT_y
Cuts at: 1493 3106 3332 3484 5593 1493
Size: 1613 226 152 2109 1697
Fragments arranged by size:
2109 1697 1613 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4351 160
Size: 872 3319 1606
Fragments arranged by size:
3319 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
AvaI C'yCGr_G
Cuts at: 1870 1943 2426 3122 1870
Size: 73 483 696 4545
Fragments arranged by size:
4545 696 483 73
BamHI G'GATC_C
Cuts at: 3127 3494 3127
Size: 367 5430
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2204 2649 3118 4573 618
Size: 325 217 627 417 445 469 1455 1842
Fragments arranged by size:
1842 1455 627 469 445 417 325 217
BanII G_rGCy'C
Cuts at: 1391 2706 3029 3116 1391
Size: 1315 323 87 4072
Fragments arranged by size:
4072 1315 323 87
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2061 2236 928
Size: 971 162 175 4489
Fragments arranged by size:
4489 971 175 162
BbvCI CC'TCA_GC
Cuts at: 2973 2973
Size: 5797
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3988 4022 1903
Size: 34 2051 34 3678
Fragments arranged by size:
3678 2051 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3689 4899 3689
Size: 1210 4587
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5797
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4408 136
Size: 107 122 71 631 605 2736 1525
Fragments arranged by size:
2736 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5792 5792
Size: 5797
BlpI GC'TnA_GC
Cuts at: 2399 3214 2399
Size: 815 4982
Bme1580I G_kGCm'C
Cuts at: 1790 2207 2537 3858 5014 1790
Size: 417 330 1321 1156 2573
Fragments arranged by size:
2573 1321 1156 417 330
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5797
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3014 3528 4486
Size: 416 53 83 399 24 1597 514 958
Cuts at: 4486 442
Size: 1753
Fragments arranged by size:
1753 1597 958 514 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5797
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2405 2720 4457 1239
Size: 1166 315 1737 2579
Fragments arranged by size:
2579 1737 1166 315
Bpu10I CC'TnA_GC
Cuts at: 2973 2973
Size: 5797
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2603 3922 4787 5085 2603
Size: 1319 865 298 3315
Fragments arranged by size:
3315 1319 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2931 3040 4460 882
Size: 617 1432 109 1420 2219
Fragments arranged by size:
2219 1432 1420 617 109
BsaAI yAC'GTr
Cuts at: 493 1204 2076 3588 493
Size: 711 872 1512 2702
Fragments arranged by size:
2702 1512 872 711
BsaBI GATnn'nnATC
Cuts at: 5791 5791
Size: 5797
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3604 3986 4621 275
Size: 53 83 186 936 2071 382 635 1451
Fragments arranged by size:
2071 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4230 4628 4902 5049 5460 4230
Size: 398 274 147 411 4567
Fragments arranged by size:
4567 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5572
Fragments arranged by size:
5572 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2182 2543 1186
Size: 996 361 4440
Fragments arranged by size:
4440 996 361
BseYI C'CCAG_C
Cuts at: 2026 2379 5000 5496 2026
Size: 353 2621 496 2327
Fragments arranged by size:
2621 2327 496 353
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5224
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3247 4008 5036 1540
Size: 275 186 1246 761 1028 2301
Fragments arranged by size:
2301 1246 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2537 2706 3029 3858 3943 5014 2537
Size: 169 323 829 85 1071 3320
Fragments arranged by size:
3320 1071 829 323 169 85
BsmI GAATG_Cn'
Cuts at: 3308 3401 3308
Size: 93 5704
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2207 2537 2706 3029 3116 3858 3943
Size: 399 417 330 169 323 87 742 85
Cuts at: 3943 5014 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 417 399 330 323 169
87 85
BspEI T'CCGG_A
Cuts at: 5460 5460
Size: 5797
BspHI T'CATG_A
Cuts at: 3686 5416 3686
Size: 1730 4067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2550 2576 3152 844
Size: 1706 26 576 3489
Fragments arranged by size:
3489 1706 576 26
BsrBI CCG'CTC
Cuts at: 1394 1823 3684 1394
Size: 429 1861 3507
Fragments arranged by size:
3507 1861 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4292 4466 65
Size: 1183 3044 174 1396
Fragments arranged by size:
3044 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4441 4628 1700
Size: 110 2631 187 2869
Fragments arranged by size:
2869 2631 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5797
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5797
BssSI C'ACGA_G
Cuts at: 3857 4869 3857
Size: 1012 4785
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5797
BstBI TT'CG_AA
Cuts at: 4601 4601
Size: 5797
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5797
BstXI CCAn_nnnn'nTGG
Cuts at: 2038 2949 2038
Size: 911 4886
BstYI r'GATC_y
Cuts at: 1972 2011 3127 3494 3879 3896 5348 5434 5446
Size: 39 1116 367 385 17 1452 86 12
Cuts at: 5446 5792 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1116 385 367 346 86 39
17 12
Bsu36I CC'TnA_GG
Cuts at: 2194 2194
Size: 5797
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5245
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5058
Fragments arranged by size:
5058 683 56
BtsI GCAGTG_nn'
Cuts at: 3313 4107 4127 3313
Size: 794 20 4983
Fragments arranged by size:
4983 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5797
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5762
DraI TTT'AAA
Cuts at: 2839 3101 2839
Size: 262 5535
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2240 4804 809
Size: 507 924 2564 1802
Fragments arranged by size:
2564 1802 924 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3244 4133 5762
Size: 54 1425 50 275 18 1414 889 1629
Cuts at: 5762 8
Size: 43
Fragments arranged by size:
1629 1425 1414 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3244 1537
Size: 275 1432 4090
Fragments arranged by size:
4090 1432 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3727 1353
Size: 2374 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2621 2924 4384 4758 4904 387
Size: 1064 138 1032 303 1460 374 146 1280
Fragments arranged by size:
1460 1280 1064 1032 374 303 146 138
EcoICRI GAG'CTC
Cuts at: 2704 3027 2704
Size: 323 5474
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2147 2405 2618 2720 3860 4457 5244
Size: 393 515 258 213 102 1140 597 787
Cuts at: 5244 5512 1239
Size: 268 1524
Fragments arranged by size:
1524 1140 787 597 515 393 268 258
213 102
EcoO109I rG'GnC_Cy
Cuts at: 3015 3015
Size: 5797
EcoRI G'AATT_C
Cuts at: 3106 3484 3106
Size: 378 5419
FspI TGC'GCA
Cuts at: 4303 4303
Size: 5797
HaeII r_GCGC'y
Cuts at: 1678 2608 4944 1678
Size: 930 2336 2531
Fragments arranged by size:
2531 2336 930
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2283 2315 4438 4470 4512
Size: 32 135 32 711 32 2123 32 42
Cuts at: 4512 4544 1373
Size: 32 2626
Fragments arranged by size:
2626 2123 711 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2481 3141 3387 669
Size: 988 281 543 660 246 3079
Fragments arranged by size:
3079 988 660 543 281 246
HindIII A'AGCT_T
Cuts at: 748 2669 748
Size: 1921 3876
HpaI GTT'AAC
Cuts at: 3387 3387
Size: 5797
KpnI G_GTAC'C
Cuts at: 2653 3122 2653
Size: 469 5328
MfeI C'AATT_G
Cuts at: 3396 3396
Size: 5797
MluI A'CGCG_T
Cuts at: 5673 5673
Size: 5797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2930 4911 5095 1305
Size: 1625 1981 184 2007
Fragments arranged by size:
2007 1981 1625 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3975
Fragments arranged by size:
3975 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3755 4114 4273 518
Size: 648 2589 359 159 2042
Fragments arranged by size:
2589 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2320 2959 3213 3890 5038 5283 1606
Size: 714 639 254 677 1148 245 2120
Fragments arranged by size:
2120 1148 714 677 639 254 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5245
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5797
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5797
NotI GC'GGCC_GC
Cuts at: 3244 3244
Size: 5797
NspI r_CATG'y
Cuts at: 1132 1229 2089 3155 4700 1132
Size: 97 860 1066 1545 2229
Fragments arranged by size:
2229 1545 1066 860 97
PasI CC'CwG_GG
Cuts at: 1348 2470 2761 1348
Size: 1122 291 4384
Fragments arranged by size:
4384 1122 291
PciI A'CATG_T
Cuts at: 4696 4696
Size: 5797
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5797
PfoI T'CCnGG_A
Cuts at: 2301 2898 3029 2301
Size: 597 131 5069
Fragments arranged by size:
5069 597 131
PmeI GTTT'AAAC
Cuts at: 3101 3101
Size: 5797
PmlI CAC'GTG
Cuts at: 2076 2076
Size: 5797
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2543 2575 3866 3898 5379 5411 2543
Size: 32 1291 32 1481 32 2929
Fragments arranged by size:
2929 1481 1291 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3015 3015
Size: 5797
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5797
PsiI TTA'TAA
Cuts at: 3367 3367
Size: 5797
PstI C_TGCA'G
Cuts at: 830 2547 3149 830
Size: 1717 602 3478
Fragments arranged by size:
3478 1717 602
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5797
PvuII CAG'CTG
Cuts at: 2320 2959 2320
Size: 639 5158
SacI G_AGCT'C
Cuts at: 2706 3029 2706
Size: 323 5474
SalI G'TCGA_C
Cuts at: 1936 3139 1936
Size: 1203 4594
SanDI GG'GwC_CC
Cuts at: 3015 3015
Size: 5797
SbfI CC_TGCA'GG
Cuts at: 3149 3149
Size: 5797
ScaI AGT'ACT
Cuts at: 4045 5757 4045
Size: 1712 4085
SexAI A'CCwGG_T
Cuts at: 2235 2235
Size: 5797
SfcI C'TryA_G
Cuts at: 826 1045 2543 2582 2916 3145 4280 4961 5152
Size: 219 1498 39 334 229 1135 681 191
Cuts at: 5152 826
Size: 1471
Fragments arranged by size:
1498 1471 1135 681 334 229 219 191
39
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5797
SmaI CCC'GGG
Cuts at: 1872 3124 1872
Size: 1252 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2618 3901 4802 5064 820
Size: 197 926 675 1283 901 262 1553
Fragments arranged by size:
1553 1283 926 901 675 262 197
SnaBI TAC'GTA
Cuts at: 493 3588 493
Size: 3095 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5797
SphI G_CATG'C
Cuts at: 1132 2089 3155 1132
Size: 957 1066 3774
Fragments arranged by size:
3774 1066 957
SspI AAT'ATT
Cuts at: 5 52 3721 5
Size: 47 3669 2081
Fragments arranged by size:
3669 2081 47
StuI AGG'CCT
Cuts at: 1517 2890 2978 1517
Size: 1373 88 4336
Fragments arranged by size:
4336 1373 88
StyI C'CwwG_G
Cuts at: 513 1065 2172 3236 513
Size: 552 1107 1064 3074
Fragments arranged by size:
3074 1107 1064 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2918 3824 3841 3994 4179 4624 325
Size: 1255 1338 906 17 153 185 445 1498
Fragments arranged by size:
1498 1338 1255 906 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4043 5755 96
Size: 274 80 33 51 1443 2066 1712 138
Fragments arranged by size:
2066 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5757 5789 5757
Size: 32 5765
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5797
XbaI T'CTAG_A
Cuts at: 3133 3133
Size: 5797
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3027 3027
Size: 5797
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5797
XmaI C'CCGG_G
Cuts at: 1870 3122 1870
Size: 1252 4545
XmnI GAAnn'nnTTC
Cuts at: 3926 3926
Size: 5797
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3605 276
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PspOMI PspXI PsrI RsrII SacII SapI SfiI
SfoI SrfI SwaI