Restriction map for
pFN21AE1325
(Circular) (Six-base) MAPSORT of: pFN21AE1325.seq Check: 593 from: 1 to: 6211
pFN21AE1325 6211 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4021 278
Size: 53 83 186 936 2485 2468
Fragments arranged by size:
2485 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2322 3554 1937
Size: 385 1232 4594
Fragments arranged by size:
4594 1232 385
Acc65I G'GTAC_C
Cuts at: 3532 3532
Size: 6211
AclI AA'CG_TT
Cuts at: 1563 4338 4711 1563
Size: 2775 373 3063
Fragments arranged by size:
3063 2775 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2258 4274 5658 5926 1632
Size: 626 2016 1384 268 1917
Fragments arranged by size:
2016 1917 1384 626 268
AfeI AGC'GCT
Cuts at: 3303 3369 3303
Size: 66 6145
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6014
AflIII A'CryG_T
Cuts at: 1203 5110 6087 1203
Size: 3907 977 1327
Fragments arranged by size:
3907 1327 977
AgeI A'CCGG_T
Cuts at: 5042 5042
Size: 6211
AhdI GACnn_n'nnGTC
Cuts at: 4940 4940
Size: 6211
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6211
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6211
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2057 2089 2057
Size: 32 6179
AlwNI CAG_nnn'CTG
Cuts at: 2305 2657 3128 3954 5526 2305
Size: 352 471 826 1572 2990
Fragments arranged by size:
2990 1572 826 471 352
ApaI G_GGCC'C
Cuts at: 2018 2422 3104 3355 3412 2018
Size: 404 682 251 57 4817
Fragments arranged by size:
4817 682 404 251 57
ApaLI G'TGCA_C
Cuts at: 2721 4268 5424 2721
Size: 1547 1156 3508
Fragments arranged by size:
3508 1547 1156
ApoI r'AATT_y
Cuts at: 1493 3327 3520 3746 3898 6007 1493
Size: 1834 193 226 152 2109 1697
Fragments arranged by size:
2109 1834 1697 226 193 152
AseI AT'TA_AT
Cuts at: 160 1032 4765 160
Size: 872 3733 1606
Fragments arranged by size:
3733 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6211
AvaI C'yCGr_G
Cuts at: 1870 1943 2033 2964 3072 3536 1870
Size: 73 90 931 108 464 4545
Fragments arranged by size:
4545 931 464 108 90 73
BamHI G'GATC_C
Cuts at: 3541 3908 3541
Size: 367 5844
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2928 3262 3532 4987 618
Size: 325 217 627 1141 334 270 1455 1842
Fragments arranged by size:
1842 1455 1141 627 334 325 270 217
BanII G_rGCy'C
Cuts at: 1391 2018 2422 2526 2649 3104 3355 3365 3392
Size: 627 404 104 123 455 251 10 27
Cuts at: 3392 3412 3530 1391
Size: 20 118 4072
Fragments arranged by size:
4072 627 455 404 251 123 118 104
27 20 10
BbeI G_GCGC'C
Cuts at: 2932 2932
Size: 6211
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5240
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4402 4436 1903
Size: 34 2465 34 3678
Fragments arranged by size:
3678 2465 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4103 5313 4103
Size: 1210 5001
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6211
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2268 2417 3099
Size: 107 122 71 631 605 596 149 682
Cuts at: 3099 4822 136
Size: 1723 1525
Fragments arranged by size:
1723 1525 682 631 605 596 149 122
107 71
BglII A'GATC_T
Cuts at: 2800 6206 2800
Size: 3406 2805
BlpI GC'TnA_GC
Cuts at: 2187 3364 3628 2187
Size: 1177 264 4770
Fragments arranged by size:
4770 1177 264
Bme1580I G_kGCm'C
Cuts at: 1790 2018 2422 2725 3104 3355 3412 4272 5428
Size: 228 404 303 379 251 57 860 1156
Cuts at: 5428 1790
Size: 2573
Fragments arranged by size:
2573 1156 860 404 379 303 251 228
57
BmgBI CAC'GTC
Cuts at: 1318 2782 1318
Size: 1464 4747
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2721 3942 4900
Size: 416 53 83 399 24 1304 1221 958
Cuts at: 4900 442
Size: 1753
Fragments arranged by size:
1753 1304 1221 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6211
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2326 2540 2326
Size: 214 5997
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2069 2202 2379 2553 2803 2924 3105 3183
Size: 830 133 177 174 250 121 181 78
Cuts at: 3183 4871 1239
Size: 1688 2579
Fragments arranged by size:
2579 1688 830 250 181 177 174 133
121 78
Bpu10I CC'TnA_GC
Cuts at: 2686 2686
Size: 6211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4336 5201 5499 4336
Size: 865 298 5048
Fragments arranged by size:
5048 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2547 2852 4874 882
Size: 617 1048 305 2022 2219
Fragments arranged by size:
2219 2022 1048 617 305
BsaAI yAC'GTr
Cuts at: 493 1204 2752 3421 4002 493
Size: 711 1548 669 581 2702
Fragments arranged by size:
2702 1548 711 669 581
BsaBI GATnn'nnATC
Cuts at: 6205 6205
Size: 6211
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2473 2853 2929 4018
Size: 53 83 186 936 940 380 76 1089
Cuts at: 4018 4400 5035 275
Size: 382 635 1451
Fragments arranged by size:
1451 1089 940 936 635 382 380 186
83 76 53
BsaWI w'CCGG_w
Cuts at: 4644 5042 5316 5463 5874 4644
Size: 398 274 147 411 4981
Fragments arranged by size:
4981 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2057 2087 1178
Size: 30 165 30 654 30 5302
Fragments arranged by size:
5302 654 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2058 2072 2532 2982 3442 1186
Size: 872 14 460 450 460 3955
Fragments arranged by size:
3955 872 460 460 450 14
BseYI C'CCAG_C
Cuts at: 2650 2668 2841 3215 5414 5910 2650
Size: 18 173 374 2199 496 2951
Fragments arranged by size:
2951 2199 496 374 173 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2447 1163
Size: 573 711 4927
Fragments arranged by size:
4927 711 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3661 4422 5450 1540
Size: 275 186 1660 761 1028 2301
Fragments arranged by size:
2301 1660 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2050 2193 2725 3365 3392 4272 4357 5428 2050
Size: 143 532 640 27 880 85 1071 2833
Fragments arranged by size:
2833 1071 880 640 532 143 85 27
BsmI GAATG_Cn'
Cuts at: 3722 3815 3722
Size: 93 6118
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2050 2193 2422 2526 2649 2725
Size: 399 228 32 143 229 104 123 76
Cuts at: 2725 3104 3355 3365 3392 3412 3530 4272 4357
Size: 379 251 10 27 20 118 742 85
Cuts at: 4357 5428 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 379 251 229 228
143 123 118 104 85 76 32 27
20 10
BspEI T'CCGG_A
Cuts at: 5874 5874
Size: 6211
BspHI T'CATG_A
Cuts at: 4100 5830 4100
Size: 1730 4481
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3566 844
Size: 2722 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2581 4098 1394
Size: 429 758 1517 3507
Fragments arranged by size:
3507 1517 758 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4706 4880 65
Size: 1183 3458 174 1396
Fragments arranged by size:
3458 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4855 5042 1700
Size: 110 3045 187 2869
Fragments arranged by size:
3045 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6211
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6211
BssSI C'ACGA_G
Cuts at: 2093 2423 2626 4271 5283 2093
Size: 330 203 1645 1012 3021
Fragments arranged by size:
3021 1645 1012 330 203
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3424 794
Size: 2630 3581
BstBI TT'CG_AA
Cuts at: 5015 5015
Size: 6211
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6211
BstYI r'GATC_y
Cuts at: 1972 2800 3541 3908 4293 4310 5762 5848 5860
Size: 828 741 367 385 17 1452 86 12
Cuts at: 5860 6206 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 828 741 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2641 513
Size: 552 1576 4083
Fragments arranged by size:
4083 1576 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3495 487
Size: 683 56 2269 3203
Fragments arranged by size:
3203 2269 683 56
BtsI GCAGTG_nn'
Cuts at: 2145 3026 3143 3727 4521 4541 2145
Size: 881 117 584 794 20 3815
Fragments arranged by size:
3815 881 794 584 117 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6211
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2134 2169 529
Size: 35 1570 35 4571
Fragments arranged by size:
4571 1570 35 35
DraI TTT'AAA
Cuts at: 3515 3515
Size: 6211
DraIII CAC_nnn'GTG
Cuts at: 2729 2729
Size: 6211
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5218 809
Size: 507 3902 1802
Fragments arranged by size:
3902 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2142 3658 4547
Size: 54 1425 50 275 18 312 1516 889
Cuts at: 4547 6176 8
Size: 1629 43
Fragments arranged by size:
1629 1516 1425 889 312 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3658 1537
Size: 275 1846 4090
Fragments arranged by size:
4090 1846 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2502 2509 3425 4141 1353
Size: 1149 7 916 716 3423
Fragments arranged by size:
3423 1149 916 716 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2249 2934 4798 5172 5318 387
Size: 1064 138 660 685 1864 374 146 1280
Fragments arranged by size:
1864 1280 1064 685 660 374 146 138
EcoICRI GAG'CTC
Cuts at: 3363 3390 3363
Size: 27 6184
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2202 2258 2379 2553 2803 2924
Size: 393 437 133 56 121 174 250 121
Cuts at: 2924 3105 3183 4274 4871 5658 5926 1239
Size: 181 78 1091 597 787 268 1524
Fragments arranged by size:
1524 1091 787 597 437 393 268 250
181 174 133 121 121 78 56
EcoNI CCTnn'n_nnAGG
Cuts at: 2084 2426 2084
Size: 342 5869
EcoO109I rG'GnC_Cy
Cuts at: 2014 2419 2564 2761 3077 3100 3101 3274 3351
Size: 405 145 197 316 23 1 173 77
Cuts at: 3351 3352 3408 3409 2014
Size: 1 56 1 4816
Fragments arranged by size:
4816 405 316 197 173 145 77 56
23 1 1 1
EcoRI G'AATT_C
Cuts at: 3327 3520 3898 3327
Size: 193 378 5640
Fragments arranged by size:
5640 378 193
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2269 2269
Size: 6211
FspI TGC'GCA
Cuts at: 4717 4717
Size: 6211
HaeII r_GCGC'y
Cuts at: 1678 2174 2932 3305 3371 5358 1678
Size: 496 758 373 66 1987 2531
Fragments arranged by size:
2531 1987 758 496 373 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4852 4884 4926 4958 1373
Size: 32 135 32 3280 32 42 32 2626
Fragments arranged by size:
3280 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3555 3801 669
Size: 988 281 1617 246 3079
Fragments arranged by size:
3079 1617 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6211
HpaI GTT'AAC
Cuts at: 3801 3801
Size: 6211
KasI G'GCGC_C
Cuts at: 2928 2928
Size: 6211
KpnI G_GTAC'C
Cuts at: 3536 3536
Size: 6211
MfeI C'AATT_G
Cuts at: 3810 3810
Size: 6211
MluI A'CGCG_T
Cuts at: 6087 6087
Size: 6211
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3480 5325 5509 1305
Size: 2175 1845 184 2007
Fragments arranged by size:
2175 2007 1845 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2144 10
Size: 54 1425 343 312 4077
Fragments arranged by size:
4077 1425 343 312 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2547 4169 4528 4687 518
Size: 648 1381 1622 359 159 2042
Fragments arranged by size:
2042 1622 1381 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2054 2161 2672 3334 3627 4304 5452 5697
Size: 448 107 511 662 293 677 1148 245
Cuts at: 5697 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 662 511 448 293 245
107
NarI GG'CG_CC
Cuts at: 2929 2929
Size: 6211
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5659
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6211
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6211
NotI GC'GGCC_GC
Cuts at: 3658 3658
Size: 6211
NspI r_CATG'y
Cuts at: 1132 1229 2744 3569 5114 1132
Size: 97 1515 825 1545 2229
Fragments arranged by size:
2229 1545 1515 825 97
PasI CC'CwG_GG
Cuts at: 1348 2587 3347 1348
Size: 1239 760 4212
Fragments arranged by size:
4212 1239 760
PciI A'CATG_T
Cuts at: 5110 5110
Size: 6211
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2657 3128 3161 1108
Size: 1549 471 33 4158
Fragments arranged by size:
4158 1549 471 33
PfoI T'CCnGG_A
Cuts at: 2085 3221 2085
Size: 1136 5075
PmeI GTTT'AAAC
Cuts at: 3515 3515
Size: 6211
PmlI CAC'GTG
Cuts at: 2752 3421 2752
Size: 669 5542
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2057 2089 4280 4312 5793 5825 2057
Size: 32 2191 32 1481 32 2443
Fragments arranged by size:
2443 2191 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2761 3077 2761
Size: 316 5895
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6211
PsiI TTA'TAA
Cuts at: 3781 3781
Size: 6211
PspOMI G'GGCC_C
Cuts at: 2014 2418 3100 3351 3408 2014
Size: 404 682 251 57 4817
Fragments arranged by size:
4817 682 404 251 57
PstI C_TGCA'G
Cuts at: 830 2152 3375 3563 830
Size: 1322 1223 188 3478
Fragments arranged by size:
3478 1322 1223 188
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6211
PvuII CAG'CTG
Cuts at: 2161 2672 3334 2161
Size: 511 662 5038
Fragments arranged by size:
5038 662 511
SacI G_AGCT'C
Cuts at: 3365 3392 3365
Size: 27 6184
SalI G'TCGA_C
Cuts at: 1936 3553 1936
Size: 1617 4594
SanDI GG'GwC_CC
Cuts at: 2761 3077 2761
Size: 316 5895
SbfI CC_TGCA'GG
Cuts at: 3563 3563
Size: 6211
ScaI AGT'ACT
Cuts at: 4459 6171 4459
Size: 1712 4499
SfcI C'TryA_G
Cuts at: 826 1045 2148 3371 3559 4694 5375 5566 826
Size: 219 1103 1223 188 1135 681 191 1471
Fragments arranged by size:
1471 1223 1135 1103 681 219 191 188
SfiI GGCCn_nnn'nGGCC
Cuts at: 2417 3099 2417
Size: 682 5529
SfoI GGC'GCC
Cuts at: 2930 2930
Size: 6211
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6211
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6211
SmaI CCC'GGG
Cuts at: 1872 2035 3074 3538 1872
Size: 163 1039 464 4545
Fragments arranged by size:
4545 1039 464 163
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2964 4315 5216 5478 820
Size: 197 926 1021 1351 901 262 1553
Fragments arranged by size:
1553 1351 1021 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4002 493
Size: 3509 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6211
SphI G_CATG'C
Cuts at: 1132 3569 1132
Size: 2437 3774
SspI AAT'ATT
Cuts at: 5 52 4135 5
Size: 47 4083 2081
Fragments arranged by size:
4083 2081 47
StuI AGG'CCT
Cuts at: 1517 2411 1517
Size: 894 5317
StyI C'CwwG_G
Cuts at: 513 1065 2199 2557 3650 513
Size: 552 1134 358 1093 3074
Fragments arranged by size:
3074 1134 1093 552 358
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2740 3193 4238 4255 4408 4593 5038
Size: 1255 1160 453 1045 17 153 185 445
Cuts at: 5038 325
Size: 1498
Fragments arranged by size:
1498 1255 1160 1045 453 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2307 4457 6169
Size: 274 80 33 51 1443 330 2150 1712
Cuts at: 6169 96
Size: 138
Fragments arranged by size:
2150 1712 1443 330 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2766 2798 6171 6203 2766
Size: 32 3373 32 2774
Fragments arranged by size:
3373 2774 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6211
XbaI T'CTAG_A
Cuts at: 3547 3547
Size: 6211
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2354 2354
Size: 6211
XhoI C'TCGA_G
Cuts at: 1943 2964 1943
Size: 1021 5190
XmaI C'CCGG_G
Cuts at: 1870 2033 3072 3536 1870
Size: 163 1039 464 4545
Fragments arranged by size:
4545 1039 464 163
XmnI GAAnn'nnTTC
Cuts at: 4340 4340
Size: 6211
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4019 276
Size: 53 83 186 936 2485 2468
Fragments arranged by size:
2485 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI
BstXI BstZ17I Bsu36I EcoRV FseI FspAI NaeI NgoMIV
NruI NsiI PacI PspXI PsrI RsrII SacII SapI
SexAI SrfI SwaI