Restriction map for
pFN21AE1319
(Circular) (Six-base) MAPSORT of: pFN21AE1319.seq Check: 1496 from: 1 to: 5644
pFN21AE1319 5644 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3454 278
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2987 1937
Size: 1050 4594
Acc65I G'GTAC_C
Cuts at: 2965 2965
Size: 5644
AclI AA'CG_TT
Cuts at: 1563 3771 4144 1563
Size: 2208 373 3063
Fragments arranged by size:
3063 2208 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2753 2795 2904 3707 5091 5359 1632
Size: 1121 42 109 803 1384 268 1917
Fragments arranged by size:
1917 1384 1121 803 268 109 42
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5447
AflIII A'CryG_T
Cuts at: 1203 2236 4543 5520 1203
Size: 1033 2307 977 1327
Fragments arranged by size:
2307 1327 1033 977
AgeI A'CCGG_T
Cuts at: 4475 4475
Size: 5644
AhdI GACnn_n'nnGTC
Cuts at: 4373 4373
Size: 5644
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5644
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5644
AlwNI CAG_nnn'CTG
Cuts at: 2104 3387 4959 2104
Size: 1283 1572 2789
Fragments arranged by size:
2789 1572 1283
ApaI G_GGCC'C
Cuts at: 2402 2402
Size: 5644
ApaLI G'TGCA_C
Cuts at: 3701 4857 3701
Size: 1156 4488
ApoI r'AATT_y
Cuts at: 1493 2213 2802 2953 3179 3331 5440 1493
Size: 720 589 151 226 152 2109 1697
Fragments arranged by size:
2109 1697 720 589 226 152 151
AseI AT'TA_AT
Cuts at: 160 1032 4198 160
Size: 872 3166 1606
Fragments arranged by size:
3166 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
AvaI C'yCGr_G
Cuts at: 1870 1943 2346 2969 1870
Size: 73 403 623 4545
Fragments arranged by size:
4545 623 403 73
BamHI G'GATC_C
Cuts at: 2285 2342 2974 3341 2285
Size: 57 632 367 4588
Fragments arranged by size:
4588 632 367 57
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2159 2165 2965 4420 618
Size: 325 217 627 372 6 800 1455 1842
Fragments arranged by size:
1842 1455 800 627 372 325 217 6
BanII G_rGCy'C
Cuts at: 1391 2402 2963 1391
Size: 1011 561 4072
Fragments arranged by size:
4072 1011 561
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2301 2892 928
Size: 971 402 591 3680
Fragments arranged by size:
3680 971 591 402
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3835 3869 1903
Size: 34 50 34 1814 34 3678
Fragments arranged by size:
3678 1814 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3536 4746 3536
Size: 1210 4434
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5644
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2035 4255 136
Size: 107 122 71 631 605 363 2220 1525
Fragments arranged by size:
2220 1525 631 605 363 122 107 71
BglII A'GATC_T
Cuts at: 2560 5639 2560
Size: 3079 2565
BlpI GC'TnA_GC
Cuts at: 3061 3061
Size: 5644
Bme1580I G_kGCm'C
Cuts at: 1790 2162 2402 3705 4861 1790
Size: 372 240 1303 1156 2573
Fragments arranged by size:
2573 1303 1156 372 240
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5644
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2396 3375 4333
Size: 416 53 83 399 24 979 979 958
Cuts at: 4333 442
Size: 1753
Fragments arranged by size:
1753 979 979 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5644
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4304 1239
Size: 3065 2579
Bpu10I CC'TnA_GC
Cuts at: 2840 2840
Size: 5644
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3769 4634 4932 3769
Size: 865 298 4481
Fragments arranged by size:
4481 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4307 882
Size: 617 2808 2219
Fragments arranged by size:
2808 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2706 3435 493
Size: 711 1502 729 2702
Fragments arranged by size:
2702 1502 729 711
BsaBI GATnn'nnATC
Cuts at: 5638 5638
Size: 5644
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2067 2136 3451 3833
Size: 53 83 186 936 534 69 1315 382
Cuts at: 3833 4468 275
Size: 635 1451
Fragments arranged by size:
1451 1315 936 635 534 382 186 83
69 53
BsaWI w'CCGG_w
Cuts at: 4077 4475 4749 4896 5307 4077
Size: 398 274 147 411 4414
Fragments arranged by size:
4414 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2084 2109 2114 2139 2255
Size: 30 165 30 681 25 5 25 116
Cuts at: 2255 2285 1178
Size: 30 4537
Fragments arranged by size:
4537 681 165 116 30 30 30 25
25 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2276 1186
Size: 1090 4554
BseYI C'CCAG_C
Cuts at: 4847 5343 4847
Size: 496 5148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5071
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2152 3094 3855 4883 1540
Size: 275 186 151 942 761 1028 2301
Fragments arranged by size:
2301 1028 942 761 275 186 151
BsiHKAI G_wGCw'C
Cuts at: 3705 3790 4861 3705
Size: 85 1071 4488
Fragments arranged by size:
4488 1071 85
BsmI GAATG_Cn'
Cuts at: 3155 3248 3155
Size: 93 5551
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2162 2402 2963 3705 3790 4861 1391
Size: 399 372 240 561 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 561 399 372 240 85
BspEI T'CCGG_A
Cuts at: 5307 5307
Size: 5644
BspHI T'CATG_A
Cuts at: 3533 5263 3533
Size: 1730 3914
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2999 844
Size: 2155 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2062 3531 1394
Size: 429 239 1469 3507
Fragments arranged by size:
3507 1469 429 239
BsrDI GCAATG_nn'
Cuts at: 65 1248 4139 4313 65
Size: 1183 2891 174 1396
Fragments arranged by size:
2891 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2015 2034 2162 4288 4475 1700
Size: 110 205 19 128 2126 187 2869
Fragments arranged by size:
2869 2126 205 187 128 110 19
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5644
BssHII G'CGCG_C
Cuts at: 1925 2192 1925
Size: 267 5377
BssSI C'ACGA_G
Cuts at: 3704 4716 3704
Size: 1012 4632
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5644
BstBI TT'CG_AA
Cuts at: 2851 4448 2851
Size: 1597 4047
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5644
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5644
BstYI r'GATC_y
Cuts at: 1972 2112 2285 2342 2560 2974 3341 3726 3743
Size: 140 173 57 218 414 367 385 17
Cuts at: 3743 5195 5281 5293 5639 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 414 385 367 346 218 173
140 86 57 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5092
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2668 487
Size: 683 56 1442 3463
Fragments arranged by size:
3463 1442 683 56
BtsI GCAGTG_nn'
Cuts at: 3160 3954 3974 3160
Size: 794 20 4830
Fragments arranged by size:
4830 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5644
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5609
DraI TTT'AAA
Cuts at: 2948 2948
Size: 5644
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4651 809
Size: 507 3335 1802
Fragments arranged by size:
3335 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 3091 3980
Size: 54 1425 50 275 18 183 1078 889
Cuts at: 3980 5609 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1078 889 275 183 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3091 1537
Size: 275 1279 4090
Fragments arranged by size:
4090 1279 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3574 1353
Size: 2221 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2070 4231 4605 4751 387
Size: 1064 138 481 2161 374 146 1280
Fragments arranged by size:
2161 1280 1064 481 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2753 2795 2904 3707 4304 5091 5359
Size: 393 1121 42 109 803 597 787 268
Cuts at: 5359 1239
Size: 1524
Fragments arranged by size:
1524 1121 803 787 597 393 268 109
42
EcoRI G'AATT_C
Cuts at: 2213 2953 3331 2213
Size: 740 378 4526
Fragments arranged by size:
4526 740 378
FspI TGC'GCA
Cuts at: 2718 4150 2718
Size: 1432 4212
HaeII r_GCGC'y
Cuts at: 1678 2579 4791 1678
Size: 901 2212 2531
Fragments arranged by size:
2531 2212 901
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2310 2342 2438 2470 4285
Size: 32 135 32 738 32 96 32 1815
Cuts at: 4285 4317 4359 4391 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1815 738 135 96 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2479 2988 3234 669
Size: 988 281 541 509 246 3079
Fragments arranged by size:
3079 988 541 509 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5644
HpaI GTT'AAC
Cuts at: 3234 3234
Size: 5644
KpnI G_GTAC'C
Cuts at: 2969 2969
Size: 5644
MfeI C'AATT_G
Cuts at: 3243 3243
Size: 5644
MluI A'CGCG_T
Cuts at: 5520 5520
Size: 5644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2140 2942 4758 4942 1305
Size: 835 802 1816 184 2007
Fragments arranged by size:
2007 1816 835 802 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3822
Fragments arranged by size:
3822 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2708 3602 3961 4120 518
Size: 648 843 699 894 359 159 2042
Fragments arranged by size:
2042 894 843 699 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2130 2638 3060 3737 4885 5130 1606
Size: 524 508 422 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 524 508 422 245
NaeI GCC'GGC
Cuts at: 2017 2036 2017
Size: 19 5625
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5092
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5644
NgoMIV G'CCGG_C
Cuts at: 2015 2034 2015
Size: 19 5625
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5644
NotI GC'GGCC_GC
Cuts at: 3091 3091
Size: 5644
NspI r_CATG'y
Cuts at: 1132 1229 2188 3002 4547 1132
Size: 97 959 814 1545 2229
Fragments arranged by size:
2229 1545 959 814 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5644
PciI A'CATG_T
Cuts at: 4543 4543
Size: 5644
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2408 1108
Size: 1300 4344
PmeI GTTT'AAAC
Cuts at: 2948 2948
Size: 5644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3713 3745 5226 5258 3713
Size: 32 1481 32 4099
Fragments arranged by size:
4099 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5644
PsiI TTA'TAA
Cuts at: 3214 3214
Size: 5644
PspOMI G'GGCC_C
Cuts at: 2398 2398
Size: 5644
PstI C_TGCA'G
Cuts at: 830 2100 2996 830
Size: 1270 896 3478
Fragments arranged by size:
3478 1270 896
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5644
PvuII CAG'CTG
Cuts at: 2638 2638
Size: 5644
SalI G'TCGA_C
Cuts at: 1936 2986 1936
Size: 1050 4594
SbfI CC_TGCA'GG
Cuts at: 2996 2996
Size: 5644
ScaI AGT'ACT
Cuts at: 3892 5604 3892
Size: 1712 3932
SfcI C'TryA_G
Cuts at: 826 1045 2096 2197 2992 4127 4808 4999 826
Size: 219 1051 101 795 1135 681 191 1471
Fragments arranged by size:
1471 1135 1051 795 681 219 191 101
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5644
SmaI CCC'GGG
Cuts at: 1872 2971 1872
Size: 1099 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3748 4649 4911 820
Size: 197 926 1805 901 262 1553
Fragments arranged by size:
1805 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3435 493
Size: 2942 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5644
SphI G_CATG'C
Cuts at: 1132 2188 3002 1132
Size: 1056 814 3774
Fragments arranged by size:
3774 1056 814
SspI AAT'ATT
Cuts at: 5 52 3568 5
Size: 47 3516 2081
Fragments arranged by size:
3516 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5644
StyI C'CwwG_G
Cuts at: 513 1065 2313 3083 513
Size: 552 1248 770 3074
Fragments arranged by size:
3074 1248 770 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3671 3688 3841 4026 4471 325
Size: 1255 2091 17 153 185 445 1498
Fragments arranged by size:
2091 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3890 5602 96
Size: 274 80 33 51 1443 1913 1712 138
Fragments arranged by size:
1913 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2255 2287 5604 5636 2255
Size: 32 3317 32 2263
Fragments arranged by size:
3317 2263 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5644
XbaI T'CTAG_A
Cuts at: 2980 2980
Size: 5644
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5644
XmaI C'CCGG_G
Cuts at: 1870 2969 1870
Size: 1099 4545
XmnI GAAnn'nnTTC
Cuts at: 2889 3773 2889
Size: 884 4760
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3452 276
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI