Restriction map for
pFN21AE1318
(Circular) (Six-base) MAPSORT of: pFN21AE1318.seq Check: 6162 from: 1 to: 8311
pFN21AE1318 8311 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6121 278
Size: 53 83 186 936 4585 2468
Fragments arranged by size:
4585 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4971 5031 5654 1937
Size: 3034 60 623 4594
Fragments arranged by size:
4594 3034 623 60
Acc65I G'GTAC_C
Cuts at: 5632 5632
Size: 8311
AclI AA'CG_TT
Cuts at: 1563 3340 6438 6811 1563
Size: 1777 3098 373 3063
Fragments arranged by size:
3098 3063 1777 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2516 2916 3105 3240 3884 4820 5257 6374
Size: 884 400 189 135 644 936 437 1117
Cuts at: 6374 7758 8026 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1117 936 884 644 437 400
268 189 135
AfeI AGC'GCT
Cuts at: 3113 3207 3113
Size: 94 8217
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8114
AflIII A'CryG_T
Cuts at: 1203 3801 7210 8187 1203
Size: 2598 3409 977 1327
Fragments arranged by size:
3409 2598 1327 977
AgeI A'CCGG_T
Cuts at: 7142 7142
Size: 8311
AhdI GACnn_n'nnGTC
Cuts at: 4154 7040 4154
Size: 2886 5425
AleI CACnn'nnGTG
Cuts at: 1166 3017 1166
Size: 1851 6460
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4805 1184
Size: 3621 4690
AlwNI CAG_nnn'CTG
Cuts at: 3148 3353 6054 7626 3148
Size: 205 2701 1572 3833
Fragments arranged by size:
3833 2701 1572 205
ApaLI G'TGCA_C
Cuts at: 5277 6368 7524 5277
Size: 1091 1156 6064
Fragments arranged by size:
6064 1156 1091
ApoI r'AATT_y
Cuts at: 1493 5342 5620 5846 5998 8107 1493
Size: 3849 278 226 152 2109 1697
Fragments arranged by size:
3849 2109 1697 278 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6865 160
Size: 872 5833 1606
Fragments arranged by size:
5833 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8311
AvaI C'yCGr_G
Cuts at: 1870 1943 4134 4240 5636 1870
Size: 73 2191 106 1396 4545
Fragments arranged by size:
4545 2191 1396 106 73
BamHI G'GATC_C
Cuts at: 4116 5181 5641 6008 4116
Size: 1065 460 367 6419
Fragments arranged by size:
6419 1065 460 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2594 5632 7087 618
Size: 325 217 627 807 3038 1455 1842
Fragments arranged by size:
3038 1842 1455 807 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2769 4043 4249 4671 5630 1391
Size: 1378 1274 206 422 959 4072
Fragments arranged by size:
4072 1378 1274 959 422 206
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2751 5508 928
Size: 971 852 2757 3731
Fragments arranged by size:
3731 2757 971 852
BbvCI CC'TCA_GC
Cuts at: 3836 4805 3836
Size: 969 7342
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3776 3810 5258 5292 6502 6536 1903
Size: 34 1839 34 1448 34 1210 34 3678
Fragments arranged by size:
3678 1839 1448 1210 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6203 7413 6203
Size: 1210 7101
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8311
BfrBI ATG'CAT
Cuts at: 3073 3073
Size: 8311
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3865 6922 136
Size: 107 122 71 631 605 2193 3057 1525
Fragments arranged by size:
3057 2193 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2714 2881 8306 2714
Size: 167 5425 2719
Fragments arranged by size:
5425 2719 167
BlpI GC'TnA_GC
Cuts at: 5458 5728 5458
Size: 270 8041
Bme1580I G_kGCm'C
Cuts at: 1790 3748 5281 6372 7528 1790
Size: 1958 1533 1091 1156 2573
Fragments arranged by size:
2573 1958 1533 1156 1091
BmgBI CAC'GTC
Cuts at: 1318 3802 5146 1318
Size: 2484 1344 4483
Fragments arranged by size:
4483 2484 1344
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4160 4933 6042
Size: 416 53 83 399 24 2743 773 1109
Cuts at: 6042 7000 442
Size: 958 1753
Fragments arranged by size:
2743 1753 1109 958 773 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 4077 1056
Size: 3021 5290
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2190 2190
Size: 8311
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2073 2386 2439 6971 1239
Size: 834 313 53 4532 2579
Fragments arranged by size:
4532 2579 834 313 53
Bpu10I CC'TnA_GC
Cuts at: 3836 4805 3836
Size: 969 7342
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2160 6436 7301 7599 2160
Size: 4276 865 298 2872
Fragments arranged by size:
4276 2872 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2131 2421 6974 882
Size: 617 632 290 4553 2219
Fragments arranged by size:
4553 2219 632 617 290
BsaAI yAC'GTr
Cuts at: 493 1204 6102 493
Size: 711 4898 2702
Fragments arranged by size:
4898 2702 711
BsaBI GATnn'nnATC
Cuts at: 3495 4775 8305 3495
Size: 1280 3530 3501
Fragments arranged by size:
3530 3501 1280
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3915 4155 4974 6118
Size: 53 83 186 936 2382 240 819 1144
Cuts at: 6118 6500 7135 275
Size: 382 635 1451
Fragments arranged by size:
2382 1451 1144 936 819 635 382 240
186 83 53
BsaWI w'CCGG_w
Cuts at: 5094 6744 7142 7416 7563 7974 5094
Size: 1650 398 274 147 411 5431
Fragments arranged by size:
5431 1650 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3620 3650 4603 4633 4814
Size: 30 165 30 2217 30 953 30 181
Cuts at: 4814 4844 1178
Size: 30 4645
Fragments arranged by size:
4645 2217 953 181 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2070 3290 3824 3992 4069 1186
Size: 884 1220 534 168 77 5428
Fragments arranged by size:
5428 1220 884 534 168 77
BseYI C'CCAG_C
Cuts at: 3747 3860 4129 4355 4597 4922 5465 7514 8010
Size: 113 269 226 242 325 543 2049 496
Cuts at: 8010 3747
Size: 4048
Fragments arranged by size:
4048 2049 543 496 325 269 242 226
113
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2982 3672 4250 4265 4775 1163
Size: 573 1246 690 578 15 510 4699
Fragments arranged by size:
4699 1246 690 578 573 510 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5761 6522 7550 1540
Size: 275 186 3760 761 1028 2301
Fragments arranged by size:
3760 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2769 4043 4671 5245 5281 5416 6372 6457 7528
Size: 1274 628 574 36 135 956 85 1071
Cuts at: 7528 2769
Size: 3552
Fragments arranged by size:
3552 1274 1071 956 628 574 135 85
36
BsmI GAATG_Cn'
Cuts at: 2396 3782 5822 5915 2396
Size: 1386 2040 93 4792
Fragments arranged by size:
4792 2040 1386 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3155 4499 3155
Size: 1344 6967
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2769 3748 4043 4249 4671 5245 5281
Size: 399 979 979 295 206 422 574 36
Cuts at: 5281 5416 5630 6372 6457 7528 1391
Size: 135 214 742 85 1071 2174
Fragments arranged by size:
2174 1071 979 979 742 574 422 399
295 214 206 135 85 36
BspEI T'CCGG_A
Cuts at: 7974 7974
Size: 8311
BspHI T'CATG_A
Cuts at: 2221 4158 6200 7930 2221
Size: 1937 2042 1730 2602
Fragments arranged by size:
2602 2042 1937 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3858 5666 844
Size: 3014 1808 3489
Fragments arranged by size:
3489 3014 1808
BsrBI CCG'CTC
Cuts at: 1394 1823 2413 6198 1394
Size: 429 590 3785 3507
Fragments arranged by size:
3785 3507 590 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5167 5563 6806 6980 65
Size: 1183 3919 396 1243 174 1396
Fragments arranged by size:
3919 1396 1243 1183 396 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3678 4260 6955 7142 1700
Size: 110 1868 582 2695 187 2869
Fragments arranged by size:
2869 2695 1868 582 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8311
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8311
BssSI C'ACGA_G
Cuts at: 2210 2701 2738 4295 4895 5322 6371 7383 2210
Size: 491 37 1557 600 427 1049 1012 3138
Fragments arranged by size:
3138 1557 1049 1012 600 491 427 37
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3136 4594 794
Size: 2342 1458 4511
Fragments arranged by size:
4511 2342 1458
BstBI TT'CG_AA
Cuts at: 7115 7115
Size: 8311
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8311
BstXI CCAn_nnnn'nTGG
Cuts at: 2476 4984 2476
Size: 2508 5803
BstYI r'GATC_y
Cuts at: 1972 2714 2860 2881 4116 4758 5181 5298 5641
Size: 742 146 21 1235 642 423 117 343
Cuts at: 5641 6008 6393 6410 7862 7948 7960 8306 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1235 742 642 423 385 367
346 343 146 117 86 21 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2068 2430 2937 3615 513
Size: 552 1003 362 507 678 5209
Fragments arranged by size:
5209 1003 678 552 507 362
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7572
Fragments arranged by size:
7572 683 56
BtsI GCAGTG_nn'
Cuts at: 3598 3660 4006 4099 5285 5827 6621 6641 3598
Size: 62 346 93 1186 542 794 20 5268
Fragments arranged by size:
5268 1186 794 542 346 93 62 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8311
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4635 4670 529
Size: 35 4071 35 4170
Fragments arranged by size:
4170 4071 35 35
DraI TTT'AAA
Cuts at: 5615 5615
Size: 8311
DraIII CAC_nnn'GTG
Cuts at: 3005 3005
Size: 8311
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4843 7318 809
Size: 507 3527 2475 1802
Fragments arranged by size:
3527 2475 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3574 3625 4426
Size: 54 1425 50 275 18 1744 51 801
Cuts at: 4426 4812 5758 6647 8276 8
Size: 386 946 889 1629 43
Fragments arranged by size:
1744 1629 1425 946 889 801 386 275
54 51 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5758 1537
Size: 275 3946 4090
Fragments arranged by size:
4090 3946 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2432 2577 2850 2955 3216 4179 6241 1353
Size: 1079 145 273 105 261 963 2062 3423
Fragments arranged by size:
3423 2062 1079 963 273 261 145 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3197 6898 7272 7418 387
Size: 1064 138 1608 3701 374 146 1280
Fragments arranged by size:
3701 1608 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2767 4041 4669 2767
Size: 1274 628 6409
Fragments arranged by size:
6409 1274 628
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2073 2386 2439 2516 2916 3105 3240
Size: 393 441 313 53 77 400 189 135
Cuts at: 3240 3884 4820 5257 6374 6971 7758 8026 1239
Size: 644 936 437 1117 597 787 268 1524
Fragments arranged by size:
1524 1117 936 787 644 597 441 437
400 393 313 268 189 135 77 53
EcoNI CCTnn'n_nnAGG
Cuts at: 2055 2055
Size: 8311
EcoO109I rG'GnC_Cy
Cuts at: 2045 2045
Size: 8311
EcoRI G'AATT_C
Cuts at: 5620 5998 5620
Size: 378 7933
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3576 5165 3576
Size: 1589 6722
FspI TGC'GCA
Cuts at: 6817 6817
Size: 8311
HaeII r_GCGC'y
Cuts at: 1678 3115 3209 4102 4280 7458 1678
Size: 1437 94 893 178 3178 2531
Fragments arranged by size:
3178 2531 1437 893 178 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3419 3451 3839 3871 3973
Size: 32 135 32 1847 32 388 32 102
Cuts at: 3973 4005 4140 4172 4502 4534 4814 4846 5149
Size: 32 135 32 330 32 280 32 303
Cuts at: 5149 5181 6952 6984 7026 7058 1373
Size: 32 1771 32 42 32 2626
Fragments arranged by size:
2626 1847 1771 388 330 303 280 135
135 102 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3358 3847 5032 5655 5901 669
Size: 988 281 1420 489 1185 623 246 3079
Fragments arranged by size:
3079 1420 1185 988 623 489 281 246
HindIII A'AGCT_T
Cuts at: 748 5401 748
Size: 4653 3658
HpaI GTT'AAC
Cuts at: 5901 5901
Size: 8311
KpnI G_GTAC'C
Cuts at: 5636 5636
Size: 8311
MfeI C'AATT_G
Cuts at: 5190 5910 5190
Size: 720 7591
MluI A'CGCG_T
Cuts at: 8187 8187
Size: 8311
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3252 4773 5559 7425 7609 1305
Size: 1947 1521 786 1866 184 2007
Fragments arranged by size:
2007 1947 1866 1521 786 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3627 4814 10
Size: 54 1425 343 1795 1187 3507
Fragments arranged by size:
3507 1795 1425 1187 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3017 5419 6269 6628 6787 518
Size: 648 1851 2402 850 359 159 2042
Fragments arranged by size:
2402 2042 1851 850 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3298 3864 3910 3970 5020 5727 6404 7552
Size: 1692 566 46 60 1050 707 677 1148
Cuts at: 7552 7797 1606
Size: 245 2120
Fragments arranged by size:
2120 1692 1148 1050 707 677 566 245
60 46
NaeI GCC'GGC
Cuts at: 3680 3680
Size: 8311
NcoI C'CATG_G
Cuts at: 513 1065 2068 2937 3615 513
Size: 552 1003 869 678 5209
Fragments arranged by size:
5209 1003 869 678 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8311
NgoMIV G'CCGG_C
Cuts at: 3678 3678
Size: 8311
NheI G'CTAG_C
Cuts at: 1052 4073 1052
Size: 3021 5290
NotI GC'GGCC_GC
Cuts at: 5758 5758
Size: 8311
NsiI A_TGCA'T
Cuts at: 3075 3075
Size: 8311
NspI r_CATG'y
Cuts at: 1132 1229 5669 7214 1132
Size: 97 4440 1545 2229
Fragments arranged by size:
4440 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2197 1348
Size: 849 7462
PciI A'CATG_T
Cuts at: 7210 7210
Size: 8311
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3199 1108
Size: 2091 6220
PfoI T'CCnGG_A
Cuts at: 3168 4818 3168
Size: 1650 6661
PmeI GTTT'AAAC
Cuts at: 5615 5615
Size: 8311
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6380 6412 7893 7925 6380
Size: 32 1481 32 6766
Fragments arranged by size:
6766 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8311
PsiI TTA'TAA
Cuts at: 5881 5881
Size: 8311
PstI C_TGCA'G
Cuts at: 830 3297 5663 830
Size: 2467 2366 3478
Fragments arranged by size:
3478 2467 2366
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8311
PvuII CAG'CTG
Cuts at: 3910 5020 3910
Size: 1110 7201
SacI G_AGCT'C
Cuts at: 2769 4043 4671 2769
Size: 1274 628 6409
Fragments arranged by size:
6409 1274 628
SalI G'TCGA_C
Cuts at: 1936 5030 5653 1936
Size: 3094 623 4594
Fragments arranged by size:
4594 3094 623
SbfI CC_TGCA'GG
Cuts at: 5663 5663
Size: 8311
ScaI AGT'ACT
Cuts at: 6559 8271 6559
Size: 1712 6599
SfcI C'TryA_G
Cuts at: 826 1045 3293 3640 5589 5659 6794 7475 7666
Size: 219 2248 347 1949 70 1135 681 191
Cuts at: 7666 826
Size: 1471
Fragments arranged by size:
2248 1949 1471 1135 681 347 219 191
70
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8311
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8311
SmaI CCC'GGG
Cuts at: 1872 5638 1872
Size: 3766 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2175 6415 7316 7578 820
Size: 197 926 232 4240 901 262 1553
Fragments arranged by size:
4240 1553 926 901 262 232 197
SnaBI TAC'GTA
Cuts at: 493 6102 493
Size: 5609 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8311
SphI G_CATG'C
Cuts at: 1132 5669 1132
Size: 4537 3774
SspI AAT'ATT
Cuts at: 5 52 6235 5
Size: 47 6183 2081
Fragments arranged by size:
6183 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8311
StyI C'CwwG_G
Cuts at: 513 1065 2068 2937 3213 3615 3708 4530 5750
Size: 552 1003 869 276 402 93 822 1220
Cuts at: 5750 513
Size: 3074
Fragments arranged by size:
3074 1220 1003 869 822 552 402 276
93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6338 6355 6508 6693 7138 325
Size: 1255 4758 17 153 185 445 1498
Fragments arranged by size:
4758 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6557 8269 96
Size: 274 80 33 51 1443 4580 1712 138
Fragments arranged by size:
4580 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2245 2277 3620 3652 4101 4133 8271 8303 2245
Size: 32 1343 32 449 32 4138 32 2253
Fragments arranged by size:
4138 2253 1343 449 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8311
XbaI T'CTAG_A
Cuts at: 4009 4862 5647 4009
Size: 853 785 6673
Fragments arranged by size:
6673 853 785
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4769 4769
Size: 8311
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8311
XmaI C'CCGG_G
Cuts at: 1870 5636 1870
Size: 3766 4545
XmnI GAAnn'nnTTC
Cuts at: 6440 6440
Size: 8311
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6119 276
Size: 53 83 186 936 4585 2468
Fragments arranged by size:
4585 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BsiWI
BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI