Restriction map for
pFN21AE1315
(Circular) (Six-base) MAPSORT of: pFN21AE1315.seq Check: 3093 from: 1 to: 5632
pFN21AE1315 5632 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3442 278
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2709 2975 1937
Size: 772 266 4594
Fragments arranged by size:
4594 772 266
Acc65I G'GTAC_C
Cuts at: 2953 2953
Size: 5632
AclI AA'CG_TT
Cuts at: 1563 3759 4132 1563
Size: 2196 373 3063
Fragments arranged by size:
3063 2196 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3695 5079 5347 1632
Size: 2063 1384 268 1917
Fragments arranged by size:
2063 1917 1384 268
AfeI AGC'GCT
Cuts at: 2761 2761
Size: 5632
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5435
AflIII A'CryG_T
Cuts at: 1203 4531 5508 1203
Size: 3328 977 1327
Fragments arranged by size:
3328 1327 977
AgeI A'CCGG_T
Cuts at: 4463 4463
Size: 5632
AhdI GACnn_n'nnGTC
Cuts at: 4361 4361
Size: 5632
AleI CACnn'nnGTG
Cuts at: 1166 2027 2417 1166
Size: 861 390 4381
Fragments arranged by size:
4381 861 390
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2698 1184
Size: 1514 4118
AlwNI CAG_nnn'CTG
Cuts at: 3375 4947 3375
Size: 1572 4060
ApaI G_GGCC'C
Cuts at: 2228 2802 2228
Size: 574 5058
ApaLI G'TGCA_C
Cuts at: 3689 4845 3689
Size: 1156 4476
ApoI r'AATT_y
Cuts at: 1493 2941 3167 3319 5428 1493
Size: 1448 226 152 2109 1697
Fragments arranged by size:
2109 1697 1448 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4186 160
Size: 872 3154 1606
Fragments arranged by size:
3154 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
AvaI C'yCGr_G
Cuts at: 1870 1943 2448 2505 2802 2916 2957 1870
Size: 73 505 57 297 114 41 4545
Fragments arranged by size:
4545 505 297 114 73 57 41
BamHI G'GATC_C
Cuts at: 2962 3329 2962
Size: 367 5265
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2195 2384 2953 4408 618
Size: 325 217 627 408 189 569 1455 1842
Fragments arranged by size:
1842 1455 627 569 408 325 217 189
BanII G_rGCy'C
Cuts at: 1391 2228 2336 2802 2896 2951 1391
Size: 837 108 466 94 55 4072
Fragments arranged by size:
4072 837 466 108 94 55
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4661
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3823 3857 1903
Size: 34 1886 34 3678
Fragments arranged by size:
3678 1886 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2101 3524 4734 2101
Size: 1423 1210 2999
Fragments arranged by size:
2999 1423 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5632
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2672 2900 4243
Size: 107 122 71 631 605 1000 228 1343
Cuts at: 4243 136
Size: 1525
Fragments arranged by size:
1525 1343 1000 631 605 228 122 107
71
BglII A'GATC_T
Cuts at: 2086 5627 2086
Size: 3541 2091
BlpI GC'TnA_GC
Cuts at: 2580 3049 2580
Size: 469 5163
Bme1580I G_kGCm'C
Cuts at: 1790 2034 2198 2228 2389 2776 2802 3693 4849
Size: 244 164 30 161 387 26 891 1156
Cuts at: 4849 1790
Size: 2573
Fragments arranged by size:
2573 1156 891 387 244 164 161 30
26
BmgBI CAC'GTC
Cuts at: 1318 2713 1318
Size: 1395 4237
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2170 3363 4321
Size: 416 53 83 399 24 753 1193 958
Cuts at: 4321 442
Size: 1753
Fragments arranged by size:
1753 1193 958 753 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5632
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2475 2771 4292 1239
Size: 1236 296 1521 2579
Fragments arranged by size:
2579 1521 1236 296
Bpu10I CC'TnA_GC
Cuts at: 2442 2442
Size: 5632
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3757 4622 4920 3757
Size: 865 298 4469
Fragments arranged by size:
4469 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4295 882
Size: 617 2796 2219
Fragments arranged by size:
2796 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3423 493
Size: 711 2219 2702
Fragments arranged by size:
2702 2219 711
BsaBI GATnn'nnATC
Cuts at: 5626 5626
Size: 5632
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3439 3821 4456 275
Size: 53 83 186 936 1906 382 635 1451
Fragments arranged by size:
1906 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2121 4065 4463 4737 4884 5295 2121
Size: 1944 398 274 147 411 2458
Fragments arranged by size:
2458 1944 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5407
Fragments arranged by size:
5407 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5632
BseYI C'CCAG_C
Cuts at: 2406 4835 5331 2406
Size: 2429 496 2707
Fragments arranged by size:
2707 2429 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2668 2893 1163
Size: 573 932 225 3902
Fragments arranged by size:
3902 932 573 225
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3082 3843 4871 1540
Size: 275 186 1081 761 1028 2301
Fragments arranged by size:
2301 1081 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3693 3778 4849 3693
Size: 85 1071 4476
Fragments arranged by size:
4476 1071 85
BsmI GAATG_Cn'
Cuts at: 3143 3236 3143
Size: 93 5539
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2198 2228 2336 2389 2776 2802
Size: 399 244 164 30 108 53 387 26
Cuts at: 2802 2896 2951 3693 3778 4849 1391
Size: 94 55 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 387 244 164 108
94 85 55 53 30 26
BspEI T'CCGG_A
Cuts at: 5295 5295
Size: 5632
BspHI T'CATG_A
Cuts at: 2631 3521 5251 2631
Size: 890 1730 3012
Fragments arranged by size:
3012 1730 890
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2987 844
Size: 2143 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3519 1394
Size: 429 1696 3507
Fragments arranged by size:
3507 1696 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2499 4127 4301 65
Size: 1183 1251 1628 174 1396
Fragments arranged by size:
1628 1396 1251 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2255 4276 4463 1700
Size: 110 445 2021 187 2869
Fragments arranged by size:
2869 2021 445 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5632
BssHII G'CGCG_C
Cuts at: 1925 2158 1925
Size: 233 5399
BssSI C'ACGA_G
Cuts at: 3692 4704 3692
Size: 1012 4620
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5632
BstBI TT'CG_AA
Cuts at: 4436 4436
Size: 5632
BstEII G'GTnAC_C
Cuts at: 1184 2587 1184
Size: 1403 4229
BstXI CCAn_nnnn'nTGG
Cuts at: 2419 2419
Size: 5632
BstYI r'GATC_y
Cuts at: 1972 2086 2130 2962 3329 3714 3731 5183 5269
Size: 114 44 832 367 385 17 1452 86
Cuts at: 5269 5281 5627 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 832 385 367 346 114 86
44 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2182 2208 2412 513
Size: 552 939 178 26 204 3733
Fragments arranged by size:
3733 939 552 204 178 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2065 2680 487
Size: 683 56 839 615 3439
Fragments arranged by size:
3439 839 683 615 56
BtsI GCAGTG_nn'
Cuts at: 3148 3942 3962 3148
Size: 794 20 4818
Fragments arranged by size:
4818 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5632
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5597
DraI TTT'AAA
Cuts at: 2936 2936
Size: 5632
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4639 809
Size: 507 3323 1802
Fragments arranged by size:
3323 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2764 3079 3968
Size: 54 1425 50 275 18 934 315 889
Cuts at: 3968 5597 8
Size: 1629 43
Fragments arranged by size:
1629 1425 934 889 315 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3079 1537
Size: 275 1267 4090
Fragments arranged by size:
4090 1267 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3562 1353
Size: 2209 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4219 4593 4739 387
Size: 1064 138 2630 374 146 1280
Fragments arranged by size:
2630 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2475 2771 3695 4292 5079 5347 1239
Size: 393 843 296 924 597 787 268 1524
Fragments arranged by size:
1524 924 843 787 597 393 296 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2603 2603
Size: 5632
EcoO109I rG'GnC_Cy
Cuts at: 2224 2225 2524 2538 2798 2799 2224
Size: 1 299 14 260 1 5057
Fragments arranged by size:
5057 299 260 14 1 1
EcoRI G'AATT_C
Cuts at: 2941 3319 2941
Size: 378 5254
FspI TGC'GCA
Cuts at: 4138 4138
Size: 5632
HaeII r_GCGC'y
Cuts at: 1678 2175 2269 2763 4779 1678
Size: 497 94 494 2016 2531
Fragments arranged by size:
2531 2016 497 494 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4273 4305 4347 4379 1373
Size: 32 135 32 2701 32 42 32 2626
Fragments arranged by size:
2701 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2572 2976 3222 669
Size: 988 281 634 404 246 3079
Fragments arranged by size:
3079 988 634 404 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5632
HpaI GTT'AAC
Cuts at: 3222 3222
Size: 5632
KpnI G_GTAC'C
Cuts at: 2957 2957
Size: 5632
MfeI C'AATT_G
Cuts at: 3231 3231
Size: 5632
MluI A'CGCG_T
Cuts at: 5508 5508
Size: 5632
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2444 4746 4930 1305
Size: 1139 2302 184 2007
Fragments arranged by size:
2302 2007 1139 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2766 10
Size: 54 1425 343 934 2876
Fragments arranged by size:
2876 1425 934 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2417 3590 3949 4108 518
Size: 648 861 390 1173 359 159 2042
Fragments arranged by size:
2042 1173 861 648 390 359 159
MspA1I CmG'CkG
Cuts at: 1606 2072 2184 2884 3048 3725 4873 5118 1606
Size: 466 112 700 164 677 1148 245 2120
Fragments arranged by size:
2120 1148 700 677 466 245 164 112
NaeI GCC'GGC
Cuts at: 2257 2257
Size: 5632
NcoI C'CATG_G
Cuts at: 513 1065 2004 2208 513
Size: 552 939 204 3937
Fragments arranged by size:
3937 939 552 204
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5632
NgoMIV G'CCGG_C
Cuts at: 2255 2255
Size: 5632
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5632
NotI GC'GGCC_GC
Cuts at: 3079 3079
Size: 5632
NspI r_CATG'y
Cuts at: 1132 1229 2345 2990 4535 1132
Size: 97 1116 645 1545 2229
Fragments arranged by size:
2229 1545 1116 645 97
PasI CC'CwG_GG
Cuts at: 1348 2743 1348
Size: 1395 4237
PciI A'CATG_T
Cuts at: 4531 4531
Size: 5632
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5632
PfoI T'CCnGG_A
Cuts at: 2849 2849
Size: 5632
PmeI GTTT'AAAC
Cuts at: 2936 2936
Size: 5632
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2501 2533 3701 3733 5214 5246 2501
Size: 32 1168 32 1481 32 2887
Fragments arranged by size:
2887 1481 1168 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2538 2538
Size: 5632
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5632
PsiI TTA'TAA
Cuts at: 3202 3202
Size: 5632
PspOMI G'GGCC_C
Cuts at: 2224 2798 2224
Size: 574 5058
PstI C_TGCA'G
Cuts at: 830 2265 2984 830
Size: 1435 719 3478
Fragments arranged by size:
3478 1435 719
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5632
PvuII CAG'CTG
Cuts at: 2072 2884 2072
Size: 812 4820
SacII CC_GC'GG
Cuts at: 2185 2185
Size: 5632
SalI G'TCGA_C
Cuts at: 1936 2974 1936
Size: 1038 4594
SbfI CC_TGCA'GG
Cuts at: 2984 2984
Size: 5632
ScaI AGT'ACT
Cuts at: 3880 5592 3880
Size: 1712 3920
SfcI C'TryA_G
Cuts at: 826 1045 2163 2261 2706 2980 4115 4796 4987
Size: 219 1118 98 445 274 1135 681 191
Cuts at: 4987 826
Size: 1471
Fragments arranged by size:
1471 1135 1118 681 445 274 219 191
98
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5632
SmaI CCC'GGG
Cuts at: 1872 2450 2959 1872
Size: 578 509 4545
Fragments arranged by size:
4545 578 509
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3736 4637 4899 820
Size: 197 926 1793 901 262 1553
Fragments arranged by size:
1793 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3423 493
Size: 2930 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5632
SphI G_CATG'C
Cuts at: 1132 2345 2990 1132
Size: 1213 645 3774
Fragments arranged by size:
3774 1213 645
SrfI GCCC'GGGC
Cuts at: 2450 2450
Size: 5632
SspI AAT'ATT
Cuts at: 5 52 3556 5
Size: 47 3504 2081
Fragments arranged by size:
3504 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5632
StyI C'CwwG_G
Cuts at: 513 1065 2004 2134 2208 2328 3071 513
Size: 552 939 130 74 120 743 3074
Fragments arranged by size:
3074 939 743 552 130 120 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3659 3676 3829 4014 4459 325
Size: 1255 2079 17 153 185 445 1498
Fragments arranged by size:
2079 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2751 3878 5590
Size: 274 80 33 51 1443 774 1127 1712
Cuts at: 5590 96
Size: 138
Fragments arranged by size:
1712 1443 1127 774 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5592 5624 5592
Size: 32 5600
Tth111I GACn'n_nGTC
Cuts at: 1316 2714 1316
Size: 1398 4234
XbaI T'CTAG_A
Cuts at: 2968 2968
Size: 5632
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2206 2386 2206
Size: 180 5452
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5632
XmaI C'CCGG_G
Cuts at: 1870 2448 2957 1870
Size: 578 509 4545
Fragments arranged by size:
4545 578 509
XmnI GAAnn'nnTTC
Cuts at: 3761 3761
Size: 5632
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3440 276
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI
SfiI SfoI SwaI