Restriction map for
pFN21AE1314
(Circular) (Six-base) MAPSORT of: pFN21AE1314.seq Check: 755 from: 1 to: 6382
pFN21AE1314 6382 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4192 278
Size: 53 83 186 936 2656 2468
Fragments arranged by size:
2656 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3725 1937
Size: 1788 4594
Acc65I G'GTAC_C
Cuts at: 3703 3703
Size: 6382
AclI AA'CG_TT
Cuts at: 1563 4509 4882 1563
Size: 2946 373 3063
Fragments arranged by size:
3063 2946 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3666 4445 5829 6097 1632
Size: 2034 779 1384 268 1917
Fragments arranged by size:
2034 1917 1384 779 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6185
AflIII A'CryG_T
Cuts at: 1203 5281 6258 1203
Size: 4078 977 1327
Fragments arranged by size:
4078 1327 977
AgeI A'CCGG_T
Cuts at: 5213 5213
Size: 6382
AhdI GACnn_n'nnGTC
Cuts at: 5111 5111
Size: 6382
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6382
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2363 1184
Size: 1179 5203
AlwNI CAG_nnn'CTG
Cuts at: 4125 5697 4125
Size: 1572 4810
ApaLI G'TGCA_C
Cuts at: 4439 5595 4439
Size: 1156 5226
ApoI r'AATT_y
Cuts at: 1493 2635 2704 2729 2904 3200 3515 3602 3639
Size: 1142 69 25 175 296 315 87 37
Cuts at: 3639 3691 3917 4069 6178 1493
Size: 52 226 152 2109 1697
Fragments arranged by size:
2109 1697 1142 315 296 226 175 152
87 69 52 37 25
AseI AT'TA_AT
Cuts at: 160 1032 4936 160
Size: 872 3904 1606
Fragments arranged by size:
3904 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6382
AvaI C'yCGr_G
Cuts at: 1870 1943 3707 1870
Size: 73 1764 4545
Fragments arranged by size:
4545 1764 73
BamHI G'GATC_C
Cuts at: 2500 3712 4079 2500
Size: 1212 367 4803
Fragments arranged by size:
4803 1212 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3703 5158 618
Size: 325 217 627 1916 1455 1842
Fragments arranged by size:
1916 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2144 2283 2418 3701 1391
Size: 753 139 135 1283 4072
Fragments arranged by size:
4072 1283 753 139 135
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2593 2702 3507 928
Size: 971 694 109 805 3803
Fragments arranged by size:
3803 971 805 694 109
BbvCI CC'TCA_GC
Cuts at: 2295 2295
Size: 6382
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4573 4607 1903
Size: 34 2636 34 3678
Fragments arranged by size:
3678 2636 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4274 5484 4274
Size: 1210 5172
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6382
BfrBI ATG'CAT
Cuts at: 2340 3314 2340
Size: 974 5408
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4993 136
Size: 107 122 71 631 605 3321 1525
Fragments arranged by size:
3321 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6377 6377
Size: 6382
BlpI GC'TnA_GC
Cuts at: 3799 3799
Size: 6382
Bme1580I G_kGCm'C
Cuts at: 1790 2274 4443 5599 1790
Size: 484 2169 1156 2573
Fragments arranged by size:
2573 2169 1156 484
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6382
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3385 4113 5071
Size: 416 53 83 399 24 1968 728 958
Cuts at: 5071 442
Size: 1753
Fragments arranged by size:
1968 1753 958 728 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6382
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2167 5042 1239
Size: 928 2875 2579
Fragments arranged by size:
2875 2579 928
Bpu10I CC'TnA_GC
Cuts at: 2295 2507 2295
Size: 212 6170
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4507 5372 5670 4507
Size: 865 298 5219
Fragments arranged by size:
5219 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3060 5045 882
Size: 617 1561 1985 2219
Fragments arranged by size:
2219 1985 1561 617
BsaAI yAC'GTr
Cuts at: 493 1204 4173 493
Size: 711 2969 2702
Fragments arranged by size:
2969 2702 711
BsaBI GATnn'nnATC
Cuts at: 6376 6376
Size: 6382
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4189 4571 5206 275
Size: 53 83 186 936 2656 382 635 1451
Fragments arranged by size:
2656 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4815 5213 5487 5634 6045 4815
Size: 398 274 147 411 5152
Fragments arranged by size:
5152 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3543 3573 1178
Size: 30 165 30 2140 30 3987
Fragments arranged by size:
3987 2140 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2773 1186
Size: 1587 4795
BseYI C'CCAG_C
Cuts at: 5585 6081 5585
Size: 496 5886
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5809
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3832 4593 5621 1540
Size: 275 186 1831 761 1028 2301
Fragments arranged by size:
2301 1831 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2949 4443 4528 5599 2949
Size: 1494 85 1071 3732
Fragments arranged by size:
3732 1494 1071 85
BsmI GAATG_Cn'
Cuts at: 3893 3986 3893
Size: 93 6289
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2144 2274 2283 2418 2949 3701 4443
Size: 399 354 130 9 135 531 752 742
Cuts at: 4443 4528 5599 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 752 742 531 399 354 135
130 85 9
BspEI T'CCGG_A
Cuts at: 6045 6045
Size: 6382
BspHI T'CATG_A
Cuts at: 4271 6001 4271
Size: 1730 4652
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3737 844
Size: 2893 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4269 1394
Size: 429 2446 3507
Fragments arranged by size:
3507 2446 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4877 5051 65
Size: 1183 3629 174 1396
Fragments arranged by size:
3629 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5026 5213 1700
Size: 110 3216 187 2869
Fragments arranged by size:
3216 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6382
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6382
BssSI C'ACGA_G
Cuts at: 4442 5454 4442
Size: 1012 5370
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6382
BstBI TT'CG_AA
Cuts at: 5186 5186
Size: 6382
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6382
BstYI r'GATC_y
Cuts at: 1972 2500 3712 4079 4464 4481 5933 6019 6031
Size: 528 1212 367 385 17 1452 86 12
Cuts at: 6031 6377 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1212 528 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5830
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2952 487
Size: 683 56 1726 3917
Fragments arranged by size:
3917 1726 683 56
BtsI GCAGTG_nn'
Cuts at: 2113 3898 4692 4712 2113
Size: 1785 794 20 3783
Fragments arranged by size:
3783 1785 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6382
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2943 2978 529
Size: 35 2379 35 3933
Fragments arranged by size:
3933 2379 35 35
DraI TTT'AAA
Cuts at: 2677 3686 2677
Size: 1009 5373
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5389 809
Size: 507 4073 1802
Fragments arranged by size:
4073 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3829 4718 6347
Size: 54 1425 50 275 18 1999 889 1629
Cuts at: 6347 8
Size: 43
Fragments arranged by size:
1999 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3829 1537
Size: 275 2017 4090
Fragments arranged by size:
4090 2017 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2442 3388 4312 1353
Size: 1089 946 924 3423
Fragments arranged by size:
3423 1089 946 924
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4969 5343 5489 387
Size: 1064 138 3380 374 146 1280
Fragments arranged by size:
3380 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2167 3666 4445 5042 5829 6097 1239
Size: 393 535 1499 779 597 787 268 1524
Fragments arranged by size:
1524 1499 787 779 597 535 393 268
EcoRI G'AATT_C
Cuts at: 3691 4069 3691
Size: 378 6004
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2532 3350 2532
Size: 818 5564
FspI TGC'GCA
Cuts at: 4888 4888
Size: 6382
HaeII r_GCGC'y
Cuts at: 1678 5529 1678
Size: 3851 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2684 2716 5023 5055 5097
Size: 32 135 32 1112 32 2307 32 42
Cuts at: 5097 5129 1373
Size: 32 2626
Fragments arranged by size:
2626 2307 1112 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3373 3726 3972 669
Size: 988 281 1435 353 246 3079
Fragments arranged by size:
3079 1435 988 353 281 246
HindIII A'AGCT_T
Cuts at: 748 2976 3096 3332 748
Size: 2228 120 236 3798
Fragments arranged by size:
3798 2228 236 120
HpaI GTT'AAC
Cuts at: 3972 3972
Size: 6382
KpnI G_GTAC'C
Cuts at: 3707 3707
Size: 6382
MfeI C'AATT_G
Cuts at: 3981 3981
Size: 6382
MluI A'CGCG_T
Cuts at: 6258 6258
Size: 6382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3172 5496 5680 1305
Size: 1867 2324 184 2007
Fragments arranged by size:
2324 2007 1867 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4560
Fragments arranged by size:
4560 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2345 3159 4340 4699 4858 518
Size: 648 1179 814 1181 359 159 2042
Fragments arranged by size:
2042 1181 1179 814 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3798 4475 5623 5868 1606
Size: 2192 677 1148 245 2120
Fragments arranged by size:
2192 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5830
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6382
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6382
NotI GC'GGCC_GC
Cuts at: 3829 3829
Size: 6382
NruI TCG'CGA
Cuts at: 2937 2937
Size: 6382
NsiI A_TGCA'T
Cuts at: 2342 3316 2342
Size: 974 5408
NspI r_CATG'y
Cuts at: 1132 1229 2894 3543 3740 5285 1132
Size: 97 1665 649 197 1545 2229
Fragments arranged by size:
2229 1665 1545 649 197 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6382
PciI A'CATG_T
Cuts at: 5281 5281
Size: 6382
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6382
PmeI GTTT'AAAC
Cuts at: 3686 3686
Size: 6382
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4451 4483 5964 5996 4451
Size: 32 1481 32 4837
Fragments arranged by size:
4837 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6382
PsiI TTA'TAA
Cuts at: 3952 3952
Size: 6382
PstI C_TGCA'G
Cuts at: 830 2135 2432 3734 830
Size: 1305 297 1302 3478
Fragments arranged by size:
3478 1305 1302 297
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6382
SalI G'TCGA_C
Cuts at: 1936 3724 1936
Size: 1788 4594
SbfI CC_TGCA'GG
Cuts at: 3734 3734
Size: 6382
ScaI AGT'ACT
Cuts at: 4630 6342 4630
Size: 1712 4670
SfcI C'TryA_G
Cuts at: 826 1045 2131 2428 3040 3730 4865 5546 5737
Size: 219 1086 297 612 690 1135 681 191
Cuts at: 5737 826
Size: 1471
Fragments arranged by size:
1471 1135 1086 690 681 612 297 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6382
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6382
SmaI CCC'GGG
Cuts at: 1872 3709 1872
Size: 1837 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4486 5387 5649 820
Size: 197 926 2543 901 262 1553
Fragments arranged by size:
2543 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4173 493
Size: 3680 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6382
SphI G_CATG'C
Cuts at: 1132 3740 1132
Size: 2608 3774
SspI AAT'ATT
Cuts at: 5 52 3008 4306 5
Size: 47 2956 1298 2081
Fragments arranged by size:
2956 2081 1298 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6382
StyI C'CwwG_G
Cuts at: 513 1065 2591 3145 3821 513
Size: 552 1526 554 676 3074
Fragments arranged by size:
3074 1526 676 554 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2752 4409 4426 4579 4764 5209 325
Size: 1255 1172 1657 17 153 185 445 1498
Fragments arranged by size:
1657 1498 1255 1172 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2070 2327 4628
Size: 274 80 33 51 1443 93 257 2301
Cuts at: 4628 6340 96
Size: 1712 138
Fragments arranged by size:
2301 1712 1443 274 257 138 93 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3543 3563 3575 3595 6342 6374 3543
Size: 20 12 20 2747 32 3551
Fragments arranged by size:
3551 2747 32 20 20 12
Tth111I GACn'n_nGTC
Cuts at: 1316 3368 1316
Size: 2052 4330
XbaI T'CTAG_A
Cuts at: 3718 3718
Size: 6382
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6382
XmaI C'CCGG_G
Cuts at: 1870 3707 1870
Size: 1837 4545
XmnI GAAnn'nnTTC
Cuts at: 2636 3333 4511 2636
Size: 697 1178 4507
Fragments arranged by size:
4507 1178 697
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4190 276
Size: 53 83 186 936 2656 2468
Fragments arranged by size:
2656 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NruI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI