Restriction map for
pFN21AE1312
(Circular) (Six-base) MAPSORT of: pFN21AE1312.seq Check: 3563 from: 1 to: 6145
pFN21AE1312 6145 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2259 2259
Size: 6145
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3955 278
Size: 53 83 186 936 2419 2468
Fragments arranged by size:
2468 2419 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3488 1937
Size: 1551 4594
Acc65I G'GTAC_C
Cuts at: 3466 3466
Size: 6145
AclI AA'CG_TT
Cuts at: 1563 4272 4645 1563
Size: 2709 373 3063
Fragments arranged by size:
3063 2709 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2034 2516 2590 2745 4208 5592 5860 1632
Size: 402 482 74 155 1463 1384 268 1917
Fragments arranged by size:
1917 1463 1384 482 402 268 155 74
AflII C'TTAA_G
Cuts at: 820 1017 2642 820
Size: 197 1625 4323
Fragments arranged by size:
4323 1625 197
AflIII A'CryG_T
Cuts at: 1203 3231 3351 5044 6021 1203
Size: 2028 120 1693 977 1327
Fragments arranged by size:
2028 1693 1327 977 120
AgeI A'CCGG_T
Cuts at: 4976 4976
Size: 6145
AhdI GACnn_n'nnGTC
Cuts at: 4874 4874
Size: 6145
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6145
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6145
AlwNI CAG_nnn'CTG
Cuts at: 3295 3888 5460 3295
Size: 593 1572 3980
Fragments arranged by size:
3980 1572 593
ApaLI G'TGCA_C
Cuts at: 3181 4202 5358 3181
Size: 1021 1156 3968
Fragments arranged by size:
3968 1156 1021
ApoI r'AATT_y
Cuts at: 1493 2843 3155 3454 3680 3832 5941 1493
Size: 1350 312 299 226 152 2109 1697
Fragments arranged by size:
2109 1697 1350 312 299 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2339 4699 160
Size: 872 1307 2360 1606
Fragments arranged by size:
2360 1606 1307 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6145
AvaI C'yCGr_G
Cuts at: 1870 1943 2903 3470 1870
Size: 73 960 567 4545
Fragments arranged by size:
4545 960 567 73
AvrII C'CTAG_G
Cuts at: 2755 2755
Size: 6145
BamHI G'GATC_C
Cuts at: 2590 3475 3842 2590
Size: 885 367 4893
Fragments arranged by size:
4893 885 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3466 4921 618
Size: 325 217 627 1679 1455 1842
Fragments arranged by size:
1842 1679 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2997 3464 1391
Size: 1606 467 4072
Fragments arranged by size:
4072 1606 467
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2504 928
Size: 971 605 4569
Fragments arranged by size:
4569 971 605
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3162 3196 4336 4370 1903
Size: 34 1225 34 1140 34 3678
Fragments arranged by size:
3678 1225 1140 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4037 5247 4037
Size: 1210 4935
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6145
BfrBI ATG'CAT
Cuts at: 2863 2863
Size: 6145
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2952 4756 136
Size: 107 122 71 631 605 1280 1804 1525
Fragments arranged by size:
1804 1525 1280 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6140 6140
Size: 6145
BlpI GC'TnA_GC
Cuts at: 3562 3562
Size: 6145
Bme1580I G_kGCm'C
Cuts at: 1790 3185 4206 5362 1790
Size: 1395 1021 1156 2573
Fragments arranged by size:
2573 1395 1156 1021
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6145
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3876 4834 442
Size: 416 53 83 399 24 2459 958 1753
Fragments arranged by size:
2459 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6145
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3125 3232 3263 4805 1239
Size: 1886 107 31 1542 2579
Fragments arranged by size:
2579 1886 1542 107 31
Bpu10I CC'TnA_GC
Cuts at: 2255 2255
Size: 6145
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2570 4270 5135 5433 2570
Size: 1700 865 298 3282
Fragments arranged by size:
3282 1700 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2237 4808 882
Size: 617 738 2571 2219
Fragments arranged by size:
2571 2219 738 617
BsaAI yAC'GTr
Cuts at: 493 1204 3936 493
Size: 711 2732 2702
Fragments arranged by size:
2732 2702 711
BsaBI GATnn'nnATC
Cuts at: 6139 6139
Size: 6145
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3952 4334 4969 275
Size: 53 83 186 936 2419 382 635 1451
Fragments arranged by size:
2419 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4578 4976 5250 5397 5808 4578
Size: 398 274 147 411 4915
Fragments arranged by size:
4915 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3205 3235 3303 3333 1178
Size: 30 165 30 1802 30 68 30 3990
Fragments arranged by size:
3990 1802 165 68 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2964 1186
Size: 1778 4367
BseYI C'CCAG_C
Cuts at: 5348 5844 5348
Size: 496 5649
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5572
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3595 4356 5384 1540
Size: 275 186 1594 761 1028 2301
Fragments arranged by size:
2301 1594 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2910 3185 4206 4291 5362 2910
Size: 275 1021 85 1071 3693
Fragments arranged by size:
3693 1071 1021 275 85
BsmI GAATG_Cn'
Cuts at: 3656 3749 3656
Size: 93 6052
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2910 2997 3185 3464 4206 4291 5362
Size: 399 1120 87 188 279 742 85 1071
Cuts at: 5362 1391
Size: 2174
Fragments arranged by size:
2174 1120 1071 742 399 279 188 87
85
BspEI T'CCGG_A
Cuts at: 5808 5808
Size: 6145
BspHI T'CATG_A
Cuts at: 2349 4034 5764 2349
Size: 1685 1730 2730
Fragments arranged by size:
2730 1730 1685
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2211 2259 3282 3500 844
Size: 1367 48 1023 218 3489
Fragments arranged by size:
3489 1367 1023 218 48
BsrBI CCG'CTC
Cuts at: 1394 1823 4032 1394
Size: 429 2209 3507
Fragments arranged by size:
3507 2209 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2794 4640 4814 65
Size: 1183 1546 1846 174 1396
Fragments arranged by size:
1846 1546 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4789 4976 1700
Size: 110 2979 187 2869
Fragments arranged by size:
2979 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6145
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6145
BssSI C'ACGA_G
Cuts at: 4205 5217 4205
Size: 1012 5133
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6145
BstBI TT'CG_AA
Cuts at: 4949 4949
Size: 6145
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6145
BstXI CCAn_nnnn'nTGG
Cuts at: 2882 2882
Size: 6145
BstYI r'GATC_y
Cuts at: 1972 2590 3325 3475 3842 4227 4244 5696 5782
Size: 618 735 150 367 385 17 1452 86
Cuts at: 5782 5794 6140 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 735 618 385 367 346 150
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5593
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3155 487
Size: 683 56 1929 3477
Fragments arranged by size:
3477 1929 683 56
BtsI GCAGTG_nn'
Cuts at: 3284 3661 4455 4475 3284
Size: 377 794 20 4954
Fragments arranged by size:
4954 794 377 20
ClaI AT'CG_AT
Cuts at: 1554 2775 1554
Size: 1221 4924
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6110
DraI TTT'AAA
Cuts at: 3449 3449
Size: 6145
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5152 809
Size: 507 3836 1802
Fragments arranged by size:
3836 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2884 3592 4481
Size: 54 1425 50 275 18 1054 708 889
Cuts at: 4481 6110 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1054 889 708 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3592 1537
Size: 275 1780 4090
Fragments arranged by size:
4090 1780 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2384 2721 2738 4075 1353
Size: 1031 337 17 1337 3423
Fragments arranged by size:
3423 1337 1031 337 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4732 5106 5252 387
Size: 1064 138 3143 374 146 1280
Fragments arranged by size:
3143 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2034 2516 2590 2745 3125 3232 3263
Size: 393 402 482 74 155 380 107 31
Cuts at: 3263 4208 4805 5592 5860 1239
Size: 945 597 787 268 1524
Fragments arranged by size:
1524 945 787 597 482 402 393 380
268 155 107 74 31
EcoNI CCTnn'n_nnAGG
Cuts at: 2351 3122 2351
Size: 771 5374
EcoO109I rG'GnC_Cy
Cuts at: 2119 2119
Size: 6145
EcoRI G'AATT_C
Cuts at: 3155 3454 3832 3155
Size: 299 378 5468
Fragments arranged by size:
5468 378 299
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2057 2413 2057
Size: 356 5789
FspI TGC'GCA
Cuts at: 4651 4651
Size: 6145
HaeII r_GCGC'y
Cuts at: 1678 2661 5292 1678
Size: 983 2631 2531
Fragments arranged by size:
2631 2531 983
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2318 2350 4786 4818 4860
Size: 32 135 32 746 32 2436 32 42
Cuts at: 4860 4892 1373
Size: 32 2626
Fragments arranged by size:
2626 2436 746 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3489 3735 669
Size: 988 281 1551 246 3079
Fragments arranged by size:
3079 1551 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2700 748
Size: 1952 4193
HpaI GTT'AAC
Cuts at: 3735 3735
Size: 6145
KpnI G_GTAC'C
Cuts at: 3470 3470
Size: 6145
MfeI C'AATT_G
Cuts at: 3744 3744
Size: 6145
MluI A'CGCG_T
Cuts at: 6021 6021
Size: 6145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2397 5259 5443 1305
Size: 1092 2862 184 2007
Fragments arranged by size:
2862 2007 1092 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2886 10
Size: 54 1425 343 1054 3269
Fragments arranged by size:
3269 1425 1054 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4103 4462 4621 518
Size: 648 2937 359 159 2042
Fragments arranged by size:
2937 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3103 3561 4238 5386 5631 1606
Size: 1497 458 677 1148 245 2120
Fragments arranged by size:
2120 1497 1148 677 458 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5593
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6145
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6145
NotI GC'GGCC_GC
Cuts at: 3592 3592
Size: 6145
NsiI A_TGCA'T
Cuts at: 2865 2865
Size: 6145
NspI r_CATG'y
Cuts at: 1132 1229 3235 3355 3503 5048 1132
Size: 97 2006 120 148 1545 2229
Fragments arranged by size:
2229 2006 1545 148 120 97
PasI CC'CwG_GG
Cuts at: 1348 2824 1348
Size: 1476 4669
PciI A'CATG_T
Cuts at: 3231 3351 5044 3231
Size: 120 1693 4332
Fragments arranged by size:
4332 1693 120
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6145
PfoI T'CCnGG_A
Cuts at: 3321 3321
Size: 6145
PmeI GTTT'AAAC
Cuts at: 3449 3449
Size: 6145
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4214 4246 5727 5759 4214
Size: 32 1481 32 4600
Fragments arranged by size:
4600 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2119 2119
Size: 6145
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6145
PsiI TTA'TAA
Cuts at: 3715 3715
Size: 6145
PstI C_TGCA'G
Cuts at: 830 3279 3408 3497 830
Size: 2449 129 89 3478
Fragments arranged by size:
3478 2449 129 89
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6145
PvuII CAG'CTG
Cuts at: 3103 3103
Size: 6145
SalI G'TCGA_C
Cuts at: 1936 3487 1936
Size: 1551 4594
SapI GCTCTTCn'nnn_
Cuts at: 2738 2738
Size: 6145
SbfI CC_TGCA'GG
Cuts at: 3497 3497
Size: 6145
ScaI AGT'ACT
Cuts at: 4393 6105 4393
Size: 1712 4433
SfcI C'TryA_G
Cuts at: 826 1045 2091 2182 2281 3275 3404 3493 4628
Size: 219 1046 91 99 994 129 89 1135
Cuts at: 4628 5309 5500 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1046 994 681 219 191 129
99 91 89
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6145
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6145
SmaI CCC'GGG
Cuts at: 1872 3472 1872
Size: 1600 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2585 2642 4249 5150 5412 820
Size: 197 926 642 57 1607 901 262 1553
Fragments arranged by size:
1607 1553 926 901 642 262 197 57
SnaBI TAC'GTA
Cuts at: 493 3936 493
Size: 3443 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6145
SphI G_CATG'C
Cuts at: 1132 3503 1132
Size: 2371 3774
SspI AAT'ATT
Cuts at: 5 52 3392 4069 5
Size: 47 3340 677 2081
Fragments arranged by size:
3340 2081 677 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6145
StyI C'CwwG_G
Cuts at: 513 1065 2755 3381 3584 513
Size: 552 1690 626 203 3074
Fragments arranged by size:
3074 1690 626 552 203
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4172 4189 4342 4527 4972 325
Size: 1255 2592 17 153 185 445 1498
Fragments arranged by size:
2592 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2514 3221 4391
Size: 274 80 33 51 1443 537 707 1170
Cuts at: 4391 6103 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1170 707 537 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6105 6137 6105
Size: 32 6113
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6145
XbaI T'CTAG_A
Cuts at: 3481 3481
Size: 6145
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2966 2966
Size: 6145
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6145
XmaI C'CCGG_G
Cuts at: 1870 3470 1870
Size: 1600 4545
XmnI GAAnn'nnTTC
Cuts at: 2393 4274 2393
Size: 1881 4264
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3953 276
Size: 53 83 186 936 2419 2468
Fragments arranged by size:
2468 2419 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BbvCI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI