Restriction map for
pFN21AE1310
(Circular) (Six-base) MAPSORT of: pFN21AE1310.seq Check: 1 from: 1 to: 5896
pFN21AE1310 5896 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3706 278
Size: 53 83 186 936 2170 2468
Fragments arranged by size:
2468 2170 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3239 1937
Size: 1302 4594
Acc65I G'GTAC_C
Cuts at: 3217 3217
Size: 5896
AclI AA'CG_TT
Cuts at: 1563 4023 4396 1563
Size: 2460 373 3063
Fragments arranged by size:
3063 2460 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2751 2898 3959 5343 5611 1632
Size: 1119 147 1061 1384 268 1917
Fragments arranged by size:
1917 1384 1119 1061 268 147
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5699
AflIII A'CryG_T
Cuts at: 1203 4795 5772 1203
Size: 3592 977 1327
Fragments arranged by size:
3592 1327 977
AgeI A'CCGG_T
Cuts at: 4727 4727
Size: 5896
AhdI GACnn_n'nnGTC
Cuts at: 4625 4625
Size: 5896
AleI CACnn'nnGTG
Cuts at: 1166 2085 1166
Size: 919 4977
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2619 2838 1184
Size: 1435 219 4242
Fragments arranged by size:
4242 1435 219
AlwNI CAG_nnn'CTG
Cuts at: 2269 2631 2974 3639 5211 2269
Size: 362 343 665 1572 2954
Fragments arranged by size:
2954 1572 665 362 343
ApaI G_GGCC'C
Cuts at: 2455 2625 2455
Size: 170 5726
ApaLI G'TGCA_C
Cuts at: 3953 5109 3953
Size: 1156 4740
ApoI r'AATT_y
Cuts at: 1493 3205 3431 3583 5692 1493
Size: 1712 226 152 2109 1697
Fragments arranged by size:
2109 1712 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4450 160
Size: 872 3418 1606
Fragments arranged by size:
3418 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5896
AvaI C'yCGr_G
Cuts at: 1870 1943 3221 1870
Size: 73 1278 4545
Fragments arranged by size:
4545 1278 73
BamHI G'GATC_C
Cuts at: 3226 3593 3226
Size: 367 5529
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2306 3187 3217 4672 618
Size: 325 217 627 519 881 30 1455 1842
Fragments arranged by size:
1842 1455 881 627 519 325 217 30
BanII G_rGCy'C
Cuts at: 1391 2391 2455 2625 2765 3215 1391
Size: 1000 64 170 140 450 4072
Fragments arranged by size:
4072 1000 450 170 140 64
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4925
BbvCI CC'TCA_GC
Cuts at: 2637 2937 2637
Size: 300 5596
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4087 4121 1903
Size: 34 2150 34 3678
Fragments arranged by size:
3678 2150 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3788 4998 3788
Size: 1210 4686
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5896
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3102 4507 136
Size: 107 122 71 631 605 1430 1405 1525
Fragments arranged by size:
1525 1430 1405 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5891 5891
Size: 5896
BlpI GC'TnA_GC
Cuts at: 3313 3313
Size: 5896
Bme1580I G_kGCm'C
Cuts at: 1790 2455 2552 2625 3192 3957 5113 1790
Size: 665 97 73 567 765 1156 2573
Fragments arranged by size:
2573 1156 765 665 567 97 73
BmgBI CAC'GTC
Cuts at: 1318 2860 1318
Size: 1542 4354
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2845 3627 4585
Size: 416 53 83 399 24 1428 782 958
Cuts at: 4585 442
Size: 1753
Fragments arranged by size:
1753 1428 958 782 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5896
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2622 2622
Size: 5896
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2405 2438 2804 3083 4556 1239
Size: 1166 33 366 279 1473 2579
Fragments arranged by size:
2579 1473 1166 366 279 33
Bpu10I CC'TnA_GC
Cuts at: 2529 2637 2937 2529
Size: 108 300 5488
Fragments arranged by size:
5488 300 108
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4021 4886 5184 4021
Size: 865 298 4733
Fragments arranged by size:
4733 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4559 882
Size: 617 3060 2219
Fragments arranged by size:
3060 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2109 2592 3687 493
Size: 711 905 483 1095 2702
Fragments arranged by size:
2702 1095 905 711 483
BsaBI GATnn'nnATC
Cuts at: 5890 5890
Size: 5896
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2519 2979 3003 3703
Size: 53 83 186 936 986 460 24 700
Cuts at: 3703 4085 4720 275
Size: 382 635 1451
Fragments arranged by size:
1451 986 936 700 635 460 382 186
83 53 24
BsaWI w'CCGG_w
Cuts at: 4329 4727 5001 5148 5559 4329
Size: 398 274 147 411 4666
Fragments arranged by size:
4666 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2529 2559 3151 3181 1178
Size: 30 165 30 1126 30 592 30 3893
Fragments arranged by size:
3893 1126 592 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2729 2807 2869 3047 1186
Size: 1543 78 62 178 4035
Fragments arranged by size:
4035 1543 178 78 62
BseYI C'CCAG_C
Cuts at: 2624 2704 2894 2974 5099 5595 2624
Size: 80 190 80 2125 496 2925
Fragments arranged by size:
2925 2125 496 190 80 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2131 1163
Size: 573 395 4928
Fragments arranged by size:
4928 573 395
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3346 4107 5135 1540
Size: 275 186 1345 761 1028 2301
Fragments arranged by size:
2301 1345 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3957 4042 5113 3957
Size: 85 1071 4740
Fragments arranged by size:
4740 1071 85
BsmI GAATG_Cn'
Cuts at: 2725 3407 3500 2725
Size: 682 93 5121
Fragments arranged by size:
5121 682 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2195 2581 2866 2195
Size: 386 285 5225
Fragments arranged by size:
5225 386 285
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2391 2455 2552 2625 2765 3192 3215
Size: 399 601 64 97 73 140 427 23
Cuts at: 3215 3957 4042 5113 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 601 427 399 140 97
85 73 64 23
BspEI T'CCGG_A
Cuts at: 5559 5559
Size: 5896
BspHI T'CATG_A
Cuts at: 3785 5515 3785
Size: 1730 4166
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3251 844
Size: 2407 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2788 3783 1394
Size: 429 965 995 3507
Fragments arranged by size:
3507 995 965 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2249 4391 4565 65
Size: 1183 1001 2142 174 1396
Fragments arranged by size:
2142 1396 1183 1001 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4540 4727 1700
Size: 110 2730 187 2869
Fragments arranged by size:
2869 2730 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5896
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5896
BssSI C'ACGA_G
Cuts at: 3956 4968 3956
Size: 1012 4884
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5896
BstBI TT'CG_AA
Cuts at: 4700 4700
Size: 5896
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5896
BstXI CCAn_nnnn'nTGG
Cuts at: 2618 2618
Size: 5896
BstYI r'GATC_y
Cuts at: 1972 3226 3593 3978 3995 5447 5533 5545 5891
Size: 1254 367 385 17 1452 86 12 346
Cuts at: 5891 1972
Size: 1977
Fragments arranged by size:
1977 1452 1254 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2497 2931 2497
Size: 434 5462
BtgI C'CryG_G
Cuts at: 513 1065 2004 2083 2958 3192 513
Size: 552 939 79 875 234 3217
Fragments arranged by size:
3217 939 875 552 234 79
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2273 487
Size: 683 56 1047 4110
Fragments arranged by size:
4110 1047 683 56
BtsI GCAGTG_nn'
Cuts at: 2580 2691 3412 4206 4226 2580
Size: 111 721 794 20 4250
Fragments arranged by size:
4250 794 721 111 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5896
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5861
DraI TTT'AAA
Cuts at: 3200 3200
Size: 5896
DraIII CAC_nnn'GTG
Cuts at: 2089 2089
Size: 5896
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4903 809
Size: 507 3587 1802
Fragments arranged by size:
3587 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 3075 3343
Size: 54 1425 50 275 18 210 1035 268
Cuts at: 3343 4232 5861 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1035 889 275 268 210 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3343 1537
Size: 275 1531 4090
Fragments arranged by size:
4090 1531 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3065 3826 1353
Size: 1712 761 3423
Fragments arranged by size:
3423 1712 761
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3149 4483 4857 5003 387
Size: 1064 138 1560 1334 374 146 1280
Fragments arranged by size:
1560 1334 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2405 2438 2751 2804 2898 3083 3959
Size: 393 773 33 313 53 94 185 876
Cuts at: 3959 4556 5343 5611 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 876 787 773 597 393 313 268
185 94 53 33
EcoO109I rG'GnC_Cy
Cuts at: 2451 2452 2460 2540 2656 2671 2801 2840 2889
Size: 1 8 80 116 15 130 39 49
Cuts at: 2889 3139 2451
Size: 250 5208
Fragments arranged by size:
5208 250 130 116 80 49 39 15
8 1
EcoRI G'AATT_C
Cuts at: 3205 3583 3205
Size: 378 5518
EcoRV GAT'ATC
Cuts at: 2016 2016
Size: 5896
FspI TGC'GCA
Cuts at: 4402 4402
Size: 5896
HaeII r_GCGC'y
Cuts at: 1678 5043 1678
Size: 3365 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2251 2283 2645 2677 4537
Size: 32 135 32 679 32 362 32 1860
Cuts at: 4537 4569 4611 4643 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1860 679 362 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3240 3486 669
Size: 988 281 1302 246 3079
Fragments arranged by size:
3079 1302 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5896
HpaI GTT'AAC
Cuts at: 3486 3486
Size: 5896
KpnI G_GTAC'C
Cuts at: 3221 3221
Size: 5896
MfeI C'AATT_G
Cuts at: 3495 3495
Size: 5896
MluI A'CGCG_T
Cuts at: 5772 5772
Size: 5896
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5010 5194 1305
Size: 3705 184 2007
Fragments arranged by size:
3705 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2042 3077 10
Size: 54 1425 343 210 1035 2829
Fragments arranged by size:
2829 1425 1035 343 210 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2085 2820 3854 4213 4372 518
Size: 648 919 735 1034 359 159 2042
Fragments arranged by size:
2042 1034 919 735 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2922 3312 3989 5137 5382 1606
Size: 1316 390 677 1148 245 2120
Fragments arranged by size:
2120 1316 1148 677 390 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2958 513
Size: 552 939 954 3451
Fragments arranged by size:
3451 954 939 552
NdeI CA'TA_TG
Cuts at: 387 3178 387
Size: 2791 3105
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5896
NotI GC'GGCC_GC
Cuts at: 3343 3343
Size: 5896
NspI r_CATG'y
Cuts at: 1132 1229 3254 4799 1132
Size: 97 2025 1545 2229
Fragments arranged by size:
2229 2025 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2455 1348
Size: 1107 4789
PciI A'CATG_T
Cuts at: 4795 4795
Size: 5896
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2631 2974 1108
Size: 1523 343 4030
Fragments arranged by size:
4030 1523 343
PfoI T'CCnGG_A
Cuts at: 2382 2829 2382
Size: 447 5449
PmeI GTTT'AAAC
Cuts at: 3200 3200
Size: 5896
PmlI CAC'GTG
Cuts at: 2109 2592 2109
Size: 483 5413
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3965 3997 5478 5510 3965
Size: 32 1481 32 4351
Fragments arranged by size:
4351 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2460 2671 2801 2889 2460
Size: 211 130 88 5467
Fragments arranged by size:
5467 211 130 88
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5896
PsiI TTA'TAA
Cuts at: 3466 3466
Size: 5896
PspOMI G'GGCC_C
Cuts at: 2451 2621 2451
Size: 170 5726
PstI C_TGCA'G
Cuts at: 830 3248 830
Size: 2418 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5896
PvuII CAG'CTG
Cuts at: 2922 2922
Size: 5896
SalI G'TCGA_C
Cuts at: 1936 3238 1936
Size: 1302 4594
SanDI GG'GwC_CC
Cuts at: 2801 2889 2801
Size: 88 5808
SbfI CC_TGCA'GG
Cuts at: 3248 3248
Size: 5896
ScaI AGT'ACT
Cuts at: 4144 5856 4144
Size: 1712 4184
SfcI C'TryA_G
Cuts at: 826 1045 2136 3244 4379 5060 5251 826
Size: 219 1091 1108 1135 681 191 1471
Fragments arranged by size:
1471 1135 1108 1091 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5896
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5896
SmaI CCC'GGG
Cuts at: 1872 3223 1872
Size: 1351 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4000 4901 5163 820
Size: 197 926 2057 901 262 1553
Fragments arranged by size:
2057 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3687 493
Size: 3194 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5896
SphI G_CATG'C
Cuts at: 1132 3254 1132
Size: 2122 3774
SspI AAT'ATT
Cuts at: 5 52 3820 5
Size: 47 3768 2081
Fragments arranged by size:
3768 2081 47
StuI AGG'CCT
Cuts at: 1517 2687 1517
Size: 1170 4726
StyI C'CwwG_G
Cuts at: 513 1065 2004 2958 3049 3335 513
Size: 552 939 954 91 286 3074
Fragments arranged by size:
3074 954 939 552 286 91
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2681 2696 3923 3940 4093 4278 4723
Size: 1255 1101 15 1227 17 153 185 445
Cuts at: 4723 325
Size: 1498
Fragments arranged by size:
1498 1255 1227 1101 445 185 153 17
15
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4142 5854 96
Size: 274 80 33 51 1443 2165 1712 138
Fragments arranged by size:
2165 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2529 2561 5856 5888 2529
Size: 32 3295 32 2537
Fragments arranged by size:
3295 2537 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5896
XbaI T'CTAG_A
Cuts at: 3232 3232
Size: 5896
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5896
XmaI C'CCGG_G
Cuts at: 1870 3221 1870
Size: 1351 4545
XmnI GAAnn'nnTTC
Cuts at: 4025 4025
Size: 5896
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3704 276
Size: 53 83 186 936 2170 2468
Fragments arranged by size:
2468 2170 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I EcoICRI EcoNI FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI
RsrII SacI SacII SapI SexAI SfiI SfoI SrfI
SwaI XcmI