Restriction map for
pFN21AE1308
(Circular) (Six-base) MAPSORT of: pFN21AE1308.seq Check: 3858 from: 1 to: 6277
pFN21AE1308 6277 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4087 278
Size: 53 83 186 936 2551 2468
Fragments arranged by size:
2551 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3620 1937
Size: 1683 4594
Acc65I G'GTAC_C
Cuts at: 3598 3598
Size: 6277
AclI AA'CG_TT
Cuts at: 1563 4404 4777 1563
Size: 2841 373 3063
Fragments arranged by size:
3063 2841 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2088 2381 2705 3422 3518 3587 4340 5724
Size: 456 293 324 717 96 69 753 1384
Cuts at: 5724 5992 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 753 717 456 324 293 268
96 69
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6080
AflIII A'CryG_T
Cuts at: 1203 5176 6153 1203
Size: 3973 977 1327
Fragments arranged by size:
3973 1327 977
AgeI A'CCGG_T
Cuts at: 5108 5108
Size: 6277
AhdI GACnn_n'nnGTC
Cuts at: 5006 5006
Size: 6277
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6277
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2553 1184
Size: 1369 4908
AlwNI CAG_nnn'CTG
Cuts at: 2167 2338 3056 4020 5592 2167
Size: 171 718 964 1572 2852
Fragments arranged by size:
2852 1572 964 718 171
ApaI G_GGCC'C
Cuts at: 2018 2018
Size: 6277
ApaLI G'TGCA_C
Cuts at: 4334 5490 4334
Size: 1156 5121
ApoI r'AATT_y
Cuts at: 1493 2590 3586 3812 3964 6073 1493
Size: 1097 996 226 152 2109 1697
Fragments arranged by size:
2109 1697 1097 996 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4831 160
Size: 872 3799 1606
Fragments arranged by size:
3799 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6277
AvaI C'yCGr_G
Cuts at: 1870 1943 3602 1870
Size: 73 1659 4545
Fragments arranged by size:
4545 1659 73
BamHI G'GATC_C
Cuts at: 3607 3974 3607
Size: 367 5910
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3598 5053 618
Size: 325 217 627 1811 1455 1842
Fragments arranged by size:
1842 1811 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2018 2025 2231 3453 3596 1391
Size: 627 7 206 1222 143 4072
Fragments arranged by size:
4072 1222 627 206 143 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3237 3498 928
Size: 971 1338 261 3707
Fragments arranged by size:
3707 1338 971 261
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4468 4502 1903
Size: 34 2531 34 3678
Fragments arranged by size:
3678 2531 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3404 4169 5379 3404
Size: 765 1210 4302
Fragments arranged by size:
4302 1210 765
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6277
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4888 136
Size: 107 122 71 631 605 3216 1525
Fragments arranged by size:
3216 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2512 6272 2512
Size: 3760 2517
BlpI GC'TnA_GC
Cuts at: 3694 3694
Size: 6277
Bme1580I G_kGCm'C
Cuts at: 1790 2018 4338 5494 1790
Size: 228 2320 1156 2573
Fragments arranged by size:
2573 2320 1156 228
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6277
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2021 2608 4008
Size: 416 53 83 399 24 604 587 1400
Cuts at: 4008 4966 442
Size: 958 1753
Fragments arranged by size:
1753 1400 958 604 587 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6277
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2722 2722
Size: 6277
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2213 2483 2788 3072 4937 1239
Size: 974 270 305 284 1865 2579
Fragments arranged by size:
2579 1865 974 305 284 270
Bpu10I CC'TnA_GC
Cuts at: 3387 3387
Size: 6277
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2826 4402 5267 5565 2826
Size: 1576 865 298 3538
Fragments arranged by size:
3538 1576 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2693 2821 4940 882
Size: 617 1194 128 2119 2219
Fragments arranged by size:
2219 2119 1194 617 128
BsaAI yAC'GTr
Cuts at: 493 1204 4068 493
Size: 711 2864 2702
Fragments arranged by size:
2864 2702 711
BsaBI GATnn'nnATC
Cuts at: 6271 6271
Size: 6277
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4084 4466 5101 275
Size: 53 83 186 936 2551 382 635 1451
Fragments arranged by size:
2551 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4710 5108 5382 5529 5940 4710
Size: 398 274 147 411 5047
Fragments arranged by size:
5047 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6052
Fragments arranged by size:
6052 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2221 2380 2708 2711 2724 2861 2887 1186
Size: 1035 159 328 3 13 137 26 4576
Fragments arranged by size:
4576 1035 328 159 137 26 13 3
BseYI C'CCAG_C
Cuts at: 2542 3056 5480 5976 2542
Size: 514 2424 496 2843
Fragments arranged by size:
2843 2424 514 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2468 3219 1163
Size: 573 732 751 4221
Fragments arranged by size:
4221 751 732 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3727 4488 5516 1540
Size: 275 186 1726 761 1028 2301
Fragments arranged by size:
2301 1726 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4338 4423 5494 4338
Size: 85 1071 5121
Fragments arranged by size:
5121 1071 85
BsmI GAATG_Cn'
Cuts at: 3788 3881 3788
Size: 93 6184
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2025 2231 3453 3596 4338 4423
Size: 399 228 7 206 1222 143 742 85
Cuts at: 4423 5494 1391
Size: 1071 2174
Fragments arranged by size:
2174 1222 1071 742 399 228 206 143
85 7
BspEI T'CCGG_A
Cuts at: 5940 5940
Size: 6277
BspHI T'CATG_A
Cuts at: 2742 4166 5896 2742
Size: 1424 1730 3123
Fragments arranged by size:
3123 1730 1424
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3632 844
Size: 2788 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4164 1394
Size: 429 2341 3507
Fragments arranged by size:
3507 2341 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4772 4946 65
Size: 1183 3524 174 1396
Fragments arranged by size:
3524 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3330 3462 4921 5108 1700
Size: 110 1520 132 1459 187 2869
Fragments arranged by size:
2869 1520 1459 187 132 110
BsrGI T'GTAC_A
Cuts at: 96 3555 96
Size: 3459 2818
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6277
BssSI C'ACGA_G
Cuts at: 2472 2707 3476 4337 5349 2472
Size: 235 769 861 1012 3400
Fragments arranged by size:
3400 1012 861 769 235
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6277
BstBI TT'CG_AA
Cuts at: 5081 5081
Size: 6277
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6277
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6277
BstYI r'GATC_y
Cuts at: 1972 2512 3607 3974 4359 4376 5828 5914 5926
Size: 540 1095 367 385 17 1452 86 12
Cuts at: 5926 6272 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1095 540 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4786
Fragments arranged by size:
4786 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5538
Fragments arranged by size:
5538 683 56
BtsI GCAGTG_nn'
Cuts at: 2179 2584 3207 3292 3793 4587 4607 2179
Size: 405 623 85 501 794 20 3849
Fragments arranged by size:
3849 794 623 501 405 85 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6277
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6242
DraI TTT'AAA
Cuts at: 3075 3487 3581 3075
Size: 412 94 5771
Fragments arranged by size:
5771 412 94
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5284 809
Size: 507 3968 1802
Fragments arranged by size:
3968 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3724 4613 6242
Size: 54 1425 50 275 18 1894 889 1629
Cuts at: 6242 8
Size: 43
Fragments arranged by size:
1894 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3724 1537
Size: 275 1912 4090
Fragments arranged by size:
4090 1912 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2051 2357 2522 2819 2873 2943 3216 3533
Size: 698 306 165 297 54 70 273 317
Cuts at: 3533 4207 1353
Size: 674 3423
Fragments arranged by size:
3423 698 674 317 306 297 273 165
70 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4864 5238 5384 387
Size: 1064 138 3275 374 146 1280
Fragments arranged by size:
3275 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2088 2213 2381 2483 2705 2788 3072
Size: 393 456 125 168 102 222 83 284
Cuts at: 3072 3422 3518 3587 4340 4937 5724 5992 1239
Size: 350 96 69 753 597 787 268 1524
Fragments arranged by size:
1524 787 753 597 456 393 350 284
268 222 168 125 102 96 83 69
EcoO109I rG'GnC_Cy
Cuts at: 3010 3317 3010
Size: 307 5970
EcoRI G'AATT_C
Cuts at: 3586 3964 3586
Size: 378 5899
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2312 3439 2312
Size: 1127 5150
FspI TGC'GCA
Cuts at: 4783 4783
Size: 6277
HaeII r_GCGC'y
Cuts at: 1678 2093 2103 5424 1678
Size: 415 10 3321 2531
Fragments arranged by size:
3321 2531 415 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2719 2751 2926 2958 3174
Size: 32 135 32 1147 32 175 32 216
Cuts at: 3174 3206 3544 3576 4918 4950 4992 5024 1373
Size: 32 338 32 1342 32 42 32 2626
Fragments arranged by size:
2626 1342 1147 338 216 175 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2241 2910 3621 3867 669
Size: 988 281 303 669 711 246 3079
Fragments arranged by size:
3079 988 711 669 303 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6277
HpaI GTT'AAC
Cuts at: 3867 3867
Size: 6277
KpnI G_GTAC'C
Cuts at: 3602 3602
Size: 6277
MfeI C'AATT_G
Cuts at: 3876 3876
Size: 6277
MluI A'CGCG_T
Cuts at: 6153 6153
Size: 6277
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5391 5575 1305
Size: 4086 184 2007
Fragments arranged by size:
4086 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4455
Fragments arranged by size:
4455 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2490 3284 4235 4594 4753 518
Size: 648 1324 794 951 359 159 2042
Fragments arranged by size:
2042 1324 951 794 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2158 3693 4370 5518 5763 1606
Size: 552 1535 677 1148 245 2120
Fragments arranged by size:
2120 1535 1148 677 552 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4786
Fragments arranged by size:
4786 939 552
NdeI CA'TA_TG
Cuts at: 387 3169 387
Size: 2782 3495
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6277
NotI GC'GGCC_GC
Cuts at: 3724 3724
Size: 6277
NspI r_CATG'y
Cuts at: 1132 1229 2887 3328 3635 5180 1132
Size: 97 1658 441 307 1545 2229
Fragments arranged by size:
2229 1658 1545 441 307 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6277
PciI A'CATG_T
Cuts at: 5176 5176
Size: 6277
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6277
PfoI T'CCnGG_A
Cuts at: 2499 2499
Size: 6277
PmeI GTTT'AAAC
Cuts at: 3581 3581
Size: 6277
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4346 4378 5859 5891 4346
Size: 32 1481 32 4732
Fragments arranged by size:
4732 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3010 3317 3010
Size: 307 5970
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6277
PsiI TTA'TAA
Cuts at: 3847 3847
Size: 6277
PspOMI G'GGCC_C
Cuts at: 2014 2014
Size: 6277
PstI C_TGCA'G
Cuts at: 830 2057 2163 2220 3629 830
Size: 1227 106 57 1409 3478
Fragments arranged by size:
3478 1409 1227 106 57
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6277
PvuII CAG'CTG
Cuts at: 2158 2158
Size: 6277
SalI G'TCGA_C
Cuts at: 1936 3619 1936
Size: 1683 4594
SapI GCTCTTCn'nnn_
Cuts at: 2051 2873 3533 2051
Size: 822 660 4795
Fragments arranged by size:
4795 822 660
SbfI CC_TGCA'GG
Cuts at: 3629 3629
Size: 6277
ScaI AGT'ACT
Cuts at: 4525 6237 4525
Size: 1712 4565
SfcI C'TryA_G
Cuts at: 826 1045 2053 2159 2216 3625 4760 5441 5632
Size: 219 1008 106 57 1409 1135 681 191
Cuts at: 5632 826
Size: 1471
Fragments arranged by size:
1471 1409 1135 1008 681 219 191 106
57
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6277
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6277
SmaI CCC'GGG
Cuts at: 1872 3604 1872
Size: 1732 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2841 4381 5282 5544 820
Size: 197 926 898 1540 901 262 1553
Fragments arranged by size:
1553 1540 926 901 898 262 197
SnaBI TAC'GTA
Cuts at: 493 4068 493
Size: 3575 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6277
SphI G_CATG'C
Cuts at: 1132 3635 1132
Size: 2503 3774
SspI AAT'ATT
Cuts at: 5 52 4201 5
Size: 47 4149 2081
Fragments arranged by size:
4149 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6277
StyI C'CwwG_G
Cuts at: 513 1065 2004 3005 3030 3716 513
Size: 552 939 1001 25 686 3074
Fragments arranged by size:
3074 1001 939 686 552 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2802 4304 4321 4474 4659 5104 325
Size: 1255 1222 1502 17 153 185 445 1498
Fragments arranged by size:
1502 1498 1255 1222 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2864 3555 4523
Size: 274 80 33 51 1443 887 691 968
Cuts at: 4523 6235 96
Size: 1712 138
Fragments arranged by size:
1712 1443 968 887 691 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6237 6269 6237
Size: 32 6245
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6277
XbaI T'CTAG_A
Cuts at: 2260 3613 2260
Size: 1353 4924
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6277
XmaI C'CCGG_G
Cuts at: 1870 3602 1870
Size: 1732 4545
XmnI GAAnn'nnTTC
Cuts at: 2320 3503 4406 2320
Size: 1183 903 4191
Fragments arranged by size:
4191 1183 903
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4085 276
Size: 53 83 186 936 2551 2468
Fragments arranged by size:
2551 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI