Restriction map for
pFN21AE1306
(Circular) (Six-base) MAPSORT of: pFN21AE1306.seq Check: 2024 from: 1 to: 5938
pFN21AE1306 5938 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2760 2958 3748 278
Size: 53 83 186 936 1224 198 790 2468
Fragments arranged by size:
2468 1224 936 790 198 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3281 1937
Size: 1344 4594
Acc65I G'GTAC_C
Cuts at: 2490 3259 2490
Size: 769 5169
AclI AA'CG_TT
Cuts at: 1563 4065 4438 1563
Size: 2502 373 3063
Fragments arranged by size:
3063 2502 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2686 4001 5385 5653 1632
Size: 1054 1315 1384 268 1917
Fragments arranged by size:
1917 1384 1315 1054 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5741
AflIII A'CryG_T
Cuts at: 1203 2646 4837 5814 1203
Size: 1443 2191 977 1327
Fragments arranged by size:
2191 1443 1327 977
AgeI A'CCGG_T
Cuts at: 4769 4769
Size: 5938
AhdI GACnn_n'nnGTC
Cuts at: 4667 4667
Size: 5938
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5938
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5938
AlwNI CAG_nnn'CTG
Cuts at: 2134 3681 5253 2134
Size: 1547 1572 2819
Fragments arranged by size:
2819 1572 1547
ApaLI G'TGCA_C
Cuts at: 3995 5151 3995
Size: 1156 4782
ApoI r'AATT_y
Cuts at: 1493 3247 3473 3625 5734 1493
Size: 1754 226 152 2109 1697
Fragments arranged by size:
2109 1754 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4492 160
Size: 872 3460 1606
Fragments arranged by size:
3460 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5938
AvaI C'yCGr_G
Cuts at: 1870 1943 3092 3263 1870
Size: 73 1149 171 4545
Fragments arranged by size:
4545 1149 171 73
BamHI G'GATC_C
Cuts at: 3268 3635 3268
Size: 367 5571
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2490 3259 4714 618
Size: 325 217 627 703 769 1455 1842
Fragments arranged by size:
1842 1455 769 703 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2565 2692 3105 3192 3257 1391
Size: 1174 127 413 87 65 4072
Fragments arranged by size:
4072 1174 413 127 87 65
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3043 928
Size: 971 1144 3823
Fragments arranged by size:
3823 1144 971
BbvCI CC'TCA_GC
Cuts at: 2216 3044 2216
Size: 828 5110
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4129 4163 1903
Size: 34 2192 34 3678
Fragments arranged by size:
3678 2192 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2976 3830 5040 2976
Size: 854 1210 3874
Fragments arranged by size:
3874 1210 854
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5938
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2418 4549 136
Size: 107 122 71 631 605 746 2131 1525
Fragments arranged by size:
2131 1525 746 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5933 5933
Size: 5938
BlpI GC'TnA_GC
Cuts at: 2982 3355 2982
Size: 373 5565
Bme1580I G_kGCm'C
Cuts at: 1790 2112 2470 3999 5155 1790
Size: 322 358 1529 1156 2573
Fragments arranged by size:
2573 1529 1156 358 322
BmgBI CAC'GTC
Cuts at: 1318 2314 1318
Size: 996 4942
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3193 3669 4627
Size: 416 53 83 399 24 1776 476 958
Cuts at: 4627 442
Size: 1753
Fragments arranged by size:
1776 1753 958 476 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5938
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2974 2974
Size: 5938
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2321 2969 4598 1239
Size: 1082 648 1629 2579
Fragments arranged by size:
2579 1629 1082 648
Bpu10I CC'TnA_GC
Cuts at: 2216 2990 3044 2216
Size: 774 54 5110
Fragments arranged by size:
5110 774 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4063 4928 5226 4063
Size: 865 298 4775
Fragments arranged by size:
4775 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2339 4601 882
Size: 617 840 2262 2219
Fragments arranged by size:
2262 2219 840 617
BsaAI yAC'GTr
Cuts at: 493 1204 2647 3729 493
Size: 711 1443 1082 2702
Fragments arranged by size:
2702 1443 1082 711
BsaBI GATnn'nnATC
Cuts at: 5932 5932
Size: 5938
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2283 2457 2757 2955
Size: 53 83 186 936 750 174 300 198
Cuts at: 2955 3745 4127 4762 275
Size: 790 382 635 1451
Fragments arranged by size:
1451 936 790 750 635 382 300 198
186 174 83 53
BsaWI w'CCGG_w
Cuts at: 4371 4769 5043 5190 5601 4371
Size: 398 274 147 411 4708
Fragments arranged by size:
4708 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3073 3103 3137 3167 1178
Size: 30 165 30 1670 30 34 30 3949
Fragments arranged by size:
3949 1670 165 34 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2701 3110 3113 3145 1186
Size: 1515 409 3 32 3979
Fragments arranged by size:
3979 1515 409 32 3
BseYI C'CCAG_C
Cuts at: 2479 3121 5141 5637 2479
Size: 642 2020 496 2780
Fragments arranged by size:
2780 2020 642 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2029 2809 1163
Size: 573 293 780 4292
Fragments arranged by size:
4292 780 573 293
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3388 4149 5177 1540
Size: 275 186 1387 761 1028 2301
Fragments arranged by size:
2301 1387 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3999 4084 5155 3999
Size: 85 1071 4782
Fragments arranged by size:
4782 1071 85
BsmI GAATG_Cn'
Cuts at: 2376 3449 3542 2376
Size: 1073 93 4772
Fragments arranged by size:
4772 1073 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2962 2962
Size: 5938
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2112 2470 2565 2692 3105 3192 3257
Size: 399 322 358 95 127 413 87 65
Cuts at: 3257 3999 4084 5155 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 413 399 358 322 127
95 87 85 65
BspEI T'CCGG_A
Cuts at: 5601 5601
Size: 5938
BspHI T'CATG_A
Cuts at: 2082 3827 5557 2082
Size: 1745 1730 2463
Fragments arranged by size:
2463 1745 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3048 3293 844
Size: 2204 245 3489
Fragments arranged by size:
3489 2204 245
BsrBI CCG'CTC
Cuts at: 1394 1823 2013 3825 1394
Size: 429 190 1812 3507
Fragments arranged by size:
3507 1812 429 190
BsrDI GCAATG_nn'
Cuts at: 65 1248 4433 4607 65
Size: 1183 3185 174 1396
Fragments arranged by size:
3185 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2151 2189 2222 2372 2736 4582 4769
Size: 110 341 38 33 150 364 1846 187
Cuts at: 4769 1700
Size: 2869
Fragments arranged by size:
2869 1846 364 341 187 150 110 38
33
BsrGI T'GTAC_A
Cuts at: 96 2253 96
Size: 2157 3781
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5938
BssSI C'ACGA_G
Cuts at: 3998 5010 3998
Size: 1012 4926
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5938
BstBI TT'CG_AA
Cuts at: 4742 4742
Size: 5938
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5938
BstXI CCAn_nnnn'nTGG
Cuts at: 2899 3112 3125 2899
Size: 213 13 5712
Fragments arranged by size:
5712 213 13
BstYI r'GATC_y
Cuts at: 1972 3268 3635 4020 4037 5489 5575 5587 5933
Size: 1296 367 385 17 1452 86 12 346
Cuts at: 5933 1972
Size: 1977
Fragments arranged by size:
1977 1452 1296 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2762 2940 2762
Size: 178 5760
BtgI C'CryG_G
Cuts at: 513 1065 2898 513
Size: 552 1833 3553
Fragments arranged by size:
3553 1833 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3161 487
Size: 683 56 1935 3264
Fragments arranged by size:
3264 1935 683 56
BtsI GCAGTG_nn'
Cuts at: 2046 2374 3454 4248 4268 2046
Size: 328 1080 794 20 3716
Fragments arranged by size:
3716 1080 794 328 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5938
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5903
DraI TTT'AAA
Cuts at: 3242 3242
Size: 5938
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2096 4945 809
Size: 507 780 2849 1802
Fragments arranged by size:
2849 1802 780 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2187 2406
Size: 54 1425 50 275 18 207 150 219
Cuts at: 2406 2431 2655 2853 3385 4274 5903 8
Size: 25 224 198 532 889 1629 43
Fragments arranged by size:
1629 1425 889 532 275 224 219 207
198 150 54 50 43 25 18
EagI C'GGCC_G
Cuts at: 1537 1812 3385 1537
Size: 275 1573 4090
Fragments arranged by size:
4090 1573 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3868 1353
Size: 2515 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3026 4525 4899 5045 387
Size: 1064 138 1437 1499 374 146 1280
Fragments arranged by size:
1499 1437 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2321 2686 2969 4001 4598 5385 5653
Size: 393 689 365 283 1032 597 787 268
Cuts at: 5653 1239
Size: 1524
Fragments arranged by size:
1524 1032 787 689 597 393 365 283
268
EcoNI CCTnn'n_nnAGG
Cuts at: 3083 3083
Size: 5938
EcoO109I rG'GnC_Cy
Cuts at: 2626 2935 3138 2626
Size: 309 203 5426
Fragments arranged by size:
5426 309 203
EcoRI G'AATT_C
Cuts at: 3247 3625 3247
Size: 378 5560
FspI TGC'GCA
Cuts at: 2731 4444 2731
Size: 1713 4225
HaeII r_GCGC'y
Cuts at: 1678 5085 1678
Size: 3407 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2679 2711 4579 4611 4653
Size: 32 135 32 1107 32 1868 32 42
Cuts at: 4653 4685 1373
Size: 32 2626
Fragments arranged by size:
2626 1868 1107 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3282 3528 669
Size: 988 281 1344 246 3079
Fragments arranged by size:
3079 1344 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5938
HpaI GTT'AAC
Cuts at: 3528 3528
Size: 5938
KpnI G_GTAC'C
Cuts at: 2494 3263 2494
Size: 769 5169
MfeI C'AATT_G
Cuts at: 3537 3537
Size: 5938
MluI A'CGCG_T
Cuts at: 5814 5814
Size: 5938
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5052 5236 1305
Size: 3747 184 2007
Fragments arranged by size:
3747 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2408 2657 10
Size: 54 1425 343 576 249 3291
Fragments arranged by size:
3291 1425 576 343 249 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3896 4255 4414 518
Size: 648 2730 359 159 2042
Fragments arranged by size:
2730 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2131 2479 2511 2680 3112 3121 3354 4031
Size: 525 348 32 169 432 9 233 677
Cuts at: 4031 5179 5424 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 525 432 348 245 233
169 32 9
NaeI GCC'GGC
Cuts at: 2191 2224 2374 2738 2191
Size: 33 150 364 5391
Fragments arranged by size:
5391 364 150 33
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5386
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5938
NgoMIV G'CCGG_C
Cuts at: 2189 2222 2372 2736 2189
Size: 33 150 364 5391
Fragments arranged by size:
5391 364 150 33
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5938
NotI GC'GGCC_GC
Cuts at: 3385 3385
Size: 5938
NspI r_CATG'y
Cuts at: 1132 1229 2329 2488 3296 4841 1132
Size: 97 1100 159 808 1545 2229
Fragments arranged by size:
2229 1545 1100 808 159 97
PasI CC'CwG_GG
Cuts at: 1348 2523 1348
Size: 1175 4763
PciI A'CATG_T
Cuts at: 4837 4837
Size: 5938
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5938
PfoI T'CCnGG_A
Cuts at: 2391 2391
Size: 5938
PmeI GTTT'AAAC
Cuts at: 3242 3242
Size: 5938
PmlI CAC'GTG
Cuts at: 2647 2647
Size: 5938
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4007 4039 5520 5552 4007
Size: 32 1481 32 4393
Fragments arranged by size:
4393 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2626 2935 3138 2626
Size: 309 203 5426
Fragments arranged by size:
5426 309 203
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5938
PsiI TTA'TAA
Cuts at: 3508 3508
Size: 5938
PstI C_TGCA'G
Cuts at: 830 2053 2160 2679 3137 3290 830
Size: 1223 107 519 458 153 3478
Fragments arranged by size:
3478 1223 519 458 153 107
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5938
PvuII CAG'CTG
Cuts at: 2131 2479 2511 2680 3121 2131
Size: 348 32 169 441 4948
Fragments arranged by size:
4948 441 348 169 32
SalI G'TCGA_C
Cuts at: 1936 3280 1936
Size: 1344 4594
SanDI GG'GwC_CC
Cuts at: 2935 2935
Size: 5938
SbfI CC_TGCA'GG
Cuts at: 3137 3290 3137
Size: 153 5785
ScaI AGT'ACT
Cuts at: 4186 5898 4186
Size: 1712 4226
SfcI C'TryA_G
Cuts at: 826 1045 2049 2156 2675 2719 3133 3286 4421
Size: 219 1004 107 519 44 414 153 1135
Cuts at: 4421 5102 5293 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1004 681 519 414 219 191
153 107 44
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5938
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5938
SmaI CCC'GGG
Cuts at: 1872 3265 1872
Size: 1393 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4042 4943 5205 820
Size: 197 926 2099 901 262 1553
Fragments arranged by size:
2099 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3729 493
Size: 3236 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5938
SphI G_CATG'C
Cuts at: 1132 2488 3296 1132
Size: 1356 808 3774
Fragments arranged by size:
3774 1356 808
SspI AAT'ATT
Cuts at: 5 52 3862 5
Size: 47 3810 2081
Fragments arranged by size:
3810 2081 47
StuI AGG'CCT
Cuts at: 1517 2387 1517
Size: 870 5068
StyI C'CwwG_G
Cuts at: 513 1065 2184 2572 2930 3377 513
Size: 552 1119 388 358 447 3074
Fragments arranged by size:
3074 1119 552 447 388 358
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2285 2519 3113 3965 3982 4135 4320
Size: 1255 705 234 594 852 17 153 185
Cuts at: 4320 4765 325
Size: 445 1498
Fragments arranged by size:
1498 1255 852 705 594 445 234 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2253 4184 5896
Size: 274 80 33 51 1443 276 1931 1712
Cuts at: 5896 96
Size: 138
Fragments arranged by size:
1931 1712 1443 276 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3073 3105 5898 5930 3073
Size: 32 2793 32 3081
Fragments arranged by size:
3081 2793 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2315 2756 1316
Size: 999 441 4498
Fragments arranged by size:
4498 999 441
XbaI T'CTAG_A
Cuts at: 3274 3274
Size: 5938
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5938
XmaI C'CCGG_G
Cuts at: 1870 3263 1870
Size: 1393 4545
XmnI GAAnn'nnTTC
Cuts at: 4067 4067
Size: 5938
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2758 2956 3746 276
Size: 53 83 186 936 1224 198 790 2468
Fragments arranged by size:
2468 1224 936 790 198 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PspOMI PspXI
PsrI RsrII SacI SacII SapI SexAI SfiI SfoI
SrfI SwaI XcmI