Restriction map for
pFN21AE1282
(Circular) (Six-base) MAPSORT of: pFN21AE1282.seq Check: 4969 from: 1 to: 7087
pFN21AE1282 7087 bp
With 182 enzymes: SgfI *
July 6, 2012 17:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2008 2008
Size: 7087
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4897 278
Size: 53 83 186 936 3361 2468
Fragments arranged by size:
3361 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4430 1937
Size: 2493 4594
Acc65I G'GTAC_C
Cuts at: 2900 4408 2900
Size: 1508 5579
AclI AA'CG_TT
Cuts at: 1563 4159 5214 5587 1563
Size: 2596 1055 373 3063
Fragments arranged by size:
3063 2596 1055 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2195 2640 3692 3749 5150 6534 6802 1632
Size: 563 445 1052 57 1401 1384 268 1917
Fragments arranged by size:
1917 1401 1384 1052 563 445 268 57
AfeI AGC'GCT
Cuts at: 2441 2455 2800 2441
Size: 14 345 6728
Fragments arranged by size:
6728 345 14
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6890
AflIII A'CryG_T
Cuts at: 1203 3285 5986 6963 1203
Size: 2082 2701 977 1327
Fragments arranged by size:
2701 2082 1327 977
AgeI A'CCGG_T
Cuts at: 5918 5918
Size: 7087
AhdI GACnn_n'nnGTC
Cuts at: 5816 5816
Size: 7087
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7087
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3950 1184
Size: 2766 4321
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3845 3877 3845
Size: 32 7055
AlwNI CAG_nnn'CTG
Cuts at: 2779 4830 6402 2779
Size: 2051 1572 3464
Fragments arranged by size:
3464 2051 1572
ApaI G_GGCC'C
Cuts at: 2585 2850 2885 2585
Size: 265 35 6787
Fragments arranged by size:
6787 265 35
ApaLI G'TGCA_C
Cuts at: 3935 4088 5144 6300 3935
Size: 153 1056 1156 4722
Fragments arranged by size:
4722 1156 1056 153
ApoI r'AATT_y
Cuts at: 1493 2147 4036 4208 4396 4622 4774 6883 1493
Size: 654 1889 172 188 226 152 2109 1697
Fragments arranged by size:
2109 1889 1697 654 226 188 172 152
AseI AT'TA_AT
Cuts at: 160 1032 5641 160
Size: 872 4609 1606
Fragments arranged by size:
4609 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7087
AvaI C'yCGr_G
Cuts at: 1870 1943 2213 2722 2894 2905 3277 3361 3562
Size: 73 270 509 172 11 372 84 201
Cuts at: 3562 4072 4412 1870
Size: 510 340 4545
Fragments arranged by size:
4545 510 509 372 340 270 201 172
84 73 11
BamHI G'GATC_C
Cuts at: 4417 4784 4417
Size: 367 6720
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2900 3411 3470 3760 4048
Size: 325 217 627 1113 511 59 290 288
Cuts at: 4048 4408 5863 618
Size: 360 1455 1842
Fragments arranged by size:
1842 1455 1113 627 511 360 325 290
288 217 59
BanII G_rGCy'C
Cuts at: 1391 2585 2645 2850 2875 2885 3278 3648 4406
Size: 1194 60 205 25 10 393 370 758
Cuts at: 4406 1391
Size: 4072
Fragments arranged by size:
4072 1194 758 393 370 205 60 25
10
BbeI G_GCGC'C
Cuts at: 3415 3474 3764 4052 3415
Size: 59 290 288 6450
Fragments arranged by size:
6450 290 288 59
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2931 928
Size: 971 1032 5084
Fragments arranged by size:
5084 1032 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2131 2165 2491 2525 5278 5312 1903
Size: 34 194 34 326 34 2753 34 3678
Fragments arranged by size:
3678 2753 326 194 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4162 4979 6189 4162
Size: 817 1210 5060
Fragments arranged by size:
5060 1210 817
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7087
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2255 2580
Size: 107 122 71 631 605 337 246 325
Cuts at: 2580 2589 4250 5698 136
Size: 9 1661 1448 1525
Fragments arranged by size:
1661 1525 1448 631 605 337 325 246
122 107 71 9
BglII A'GATC_T
Cuts at: 7082 7082
Size: 7087
BlpI GC'TnA_GC
Cuts at: 3068 4504 3068
Size: 1436 5651
Bme1580I G_kGCm'C
Cuts at: 1790 2585 2757 2850 2885 3468 3771 3905 3939
Size: 795 172 93 35 583 303 134 34
Cuts at: 3939 4092 4190 5148 6304 1790
Size: 153 98 958 1156 2573
Fragments arranged by size:
2573 1156 958 795 583 303 172 153
134 98 93 35 34
BmgBI CAC'GTC
Cuts at: 1318 2922 1318
Size: 1604 5483
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3971 4818 5776
Size: 416 53 83 399 24 2554 847 958
Cuts at: 5776 442
Size: 1753
Fragments arranged by size:
2554 1753 958 847 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7087
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3446 3446
Size: 7087
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2456 3277 3398 5747 1239
Size: 1217 821 121 2349 2579
Fragments arranged by size:
2579 2349 1217 821 121
Bpu10I CC'TnA_GC
Cuts at: 2259 2450 2259
Size: 191 6896
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5212 6077 6375 5212
Size: 865 298 5924
Fragments arranged by size:
5924 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3417 4374 5750 882
Size: 617 1918 957 1376 2219
Fragments arranged by size:
2219 1918 1376 957 617
BsaAI yAC'GTr
Cuts at: 493 1204 4022 4878 493
Size: 711 2818 856 2702
Fragments arranged by size:
2818 2702 856 711
BsaBI GATnn'nnATC
Cuts at: 7081 7081
Size: 7087
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2666 2675 2855 3216
Size: 53 83 186 936 1133 9 180 361
Cuts at: 3216 3412 3471 3501 3761 4049 4894 5276 5911
Size: 196 59 30 260 288 845 382 635
Cuts at: 5911 275
Size: 1451
Fragments arranged by size:
1451 1133 936 845 635 382 361 288
260 196 186 180 83 59 53 30
9
BsaWI w'CCGG_w
Cuts at: 3640 3822 4105 5520 5918 6192 6339 6750 3640
Size: 182 283 1415 398 274 147 411 3977
Fragments arranged by size:
3977 1415 411 398 283 274 182 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2192 2222 2845 2875 3011
Size: 30 165 30 789 30 623 30 136
Cuts at: 3011 3041 3206 3236 3275 3305 1178
Size: 30 165 30 39 30 4960
Fragments arranged by size:
4960 789 623 165 165 136 39 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2627 3110 1186
Size: 1441 483 5163
Fragments arranged by size:
5163 1441 483
BseYI C'CCAG_C
Cuts at: 2742 3193 3486 3551 3676 4199 4274 4352 6290
Size: 451 293 65 125 523 75 78 1938
Cuts at: 6290 6786 2742
Size: 496 3043
Fragments arranged by size:
3043 1938 523 496 451 293 125 78
75 65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3136 4126 1163
Size: 573 1400 990 4124
Fragments arranged by size:
4124 1400 990 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2206 2295 3349 4537 5298 6326
Size: 275 186 205 89 1054 1188 761 1028
Cuts at: 6326 1540
Size: 2301
Fragments arranged by size:
2301 1188 1054 1028 761 275 205 186
89
BsiHKAI G_wGCw'C
Cuts at: 3011 3939 4092 5148 5233 6304 3011
Size: 928 153 1056 85 1071 3794
Fragments arranged by size:
3794 1071 1056 928 153 85
BsmI GAATG_Cn'
Cuts at: 4598 4691 4598
Size: 93 6994
BsmBI CGTCTCn'nnnn_
Cuts at: 2682 3226 2682
Size: 544 6543
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2585 2645 2757 2850 2875 2885 3011
Size: 399 795 60 112 93 25 10 126
Cuts at: 3011 3278 3468 3648 3771 3905 3939 4092 4190
Size: 267 190 180 123 134 34 153 98
Cuts at: 4190 4406 5148 5233 6304 1391
Size: 216 742 85 1071 2174
Fragments arranged by size:
2174 1071 795 742 399 267 216 190
180 153 134 126 123 112 98 93
85 60 34 25 10
BspEI T'CCGG_A
Cuts at: 3640 4105 6750 3640
Size: 465 2645 3977
Fragments arranged by size:
3977 2645 465
BspHI T'CATG_A
Cuts at: 4976 6706 4976
Size: 1730 5357
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2008 2067 2772 3435 4442 844
Size: 1164 59 705 663 1007 3489
Fragments arranged by size:
3489 1164 1007 705 663 59
BsrBI CCG'CTC
Cuts at: 1394 1823 2208 3875 4974 1394
Size: 429 385 1667 1099 3507
Fragments arranged by size:
3507 1667 1099 429 385
BsrDI GCAATG_nn'
Cuts at: 65 1248 5582 5756 65
Size: 1183 4334 174 1396
Fragments arranged by size:
4334 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2527 3985 4256 5731 5918 1700
Size: 110 717 1458 271 1475 187 2869
Fragments arranged by size:
2869 1475 1458 717 271 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7087
BssHII G'CGCG_C
Cuts at: 1925 2165 3666 1925
Size: 240 1501 5346
Fragments arranged by size:
5346 1501 240
BssSI C'ACGA_G
Cuts at: 5147 6159 5147
Size: 1012 6075
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2779 4149 794
Size: 1985 1370 3732
Fragments arranged by size:
3732 1985 1370
BstBI TT'CG_AA
Cuts at: 5891 5891
Size: 7087
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7087
BstXI CCAn_nnnn'nTGG
Cuts at: 2393 2575 3529 2393
Size: 182 954 5951
Fragments arranged by size:
5951 954 182
BstYI r'GATC_y
Cuts at: 1972 4417 4784 5169 5186 6638 6724 6736 7082
Size: 2445 367 385 17 1452 86 12 346
Cuts at: 7082 1972
Size: 1977
Fragments arranged by size:
2445 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2110 2487 2842 3270 4116 513
Size: 552 1045 377 355 428 846 3484
Fragments arranged by size:
3484 1045 846 552 428 377 355
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6348
Fragments arranged by size:
6348 683 56
BtsI GCAGTG_nn'
Cuts at: 3522 3707 3945 4603 5397 5417 3522
Size: 185 238 658 794 20 5192
Fragments arranged by size:
5192 794 658 238 185 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7087
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4193 4228 529
Size: 35 3629 35 3388
Fragments arranged by size:
3629 3388 35 35
DraI TTT'AAA
Cuts at: 4391 4391
Size: 7087
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2950 6094 809
Size: 507 1634 3144 1802
Fragments arranged by size:
3144 1802 1634 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2203 2292 2793
Size: 54 1425 50 275 18 373 89 501
Cuts at: 2793 2916 3060 3309 3346 3870 4119 4534 5423
Size: 123 144 249 37 524 249 415 889
Cuts at: 5423 7052 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 524 501 415 373 275
249 249 144 123 89 54 50 43
37 18
EagI C'GGCC_G
Cuts at: 1537 1812 2203 2292 3346 4534 1537
Size: 275 391 89 1054 1188 4090
Fragments arranged by size:
4090 1188 1054 391 275 89
EarI CTCTTCn'nnn_
Cuts at: 1353 2953 5017 1353
Size: 1600 2064 3423
Fragments arranged by size:
3423 2064 1600
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2748 3733 3774 3780 5674 6048
Size: 1064 138 1159 985 41 6 1894 374
Cuts at: 6048 6194 387
Size: 146 1280
Fragments arranged by size:
1894 1280 1159 1064 985 374 146 138
41 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2195 2456 2640 3277 3398 3692 3749
Size: 393 563 261 184 637 121 294 57
Cuts at: 3749 5150 5747 6534 6802 1239
Size: 1401 597 787 268 1524
Fragments arranged by size:
1524 1401 787 637 597 563 393 294
268 261 184 121 57
EcoO109I rG'GnC_Cy
Cuts at: 2581 2618 2738 2847 2881 2882 3055 3352 3573
Size: 37 120 109 34 1 173 297 221
Cuts at: 3573 3809 4030 2581
Size: 236 221 5638
Fragments arranged by size:
5638 297 236 221 221 173 120 109
37 34 1
EcoRI G'AATT_C
Cuts at: 2147 4396 4774 2147
Size: 2249 378 4460
Fragments arranged by size:
4460 2249 378
FspI TGC'GCA
Cuts at: 3083 3623 5593 3083
Size: 540 1970 4577
Fragments arranged by size:
4577 1970 540
HaeII r_GCGC'y
Cuts at: 1678 2136 2443 2457 2802 3415 3474 3728 3764
Size: 458 307 14 345 613 59 254 36
Cuts at: 3764 4009 4052 4068 6234 1678
Size: 245 43 16 2166 2531
Fragments arranged by size:
2531 2166 613 458 345 307 254 245
59 43 36 16 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2192 2224 2845 2877 3206
Size: 32 135 32 620 32 621 32 329
Cuts at: 3206 3238 4221 4253 5728 5760 5802 5834 1373
Size: 32 983 32 1475 32 42 32 2626
Fragments arranged by size:
2626 1475 983 621 620 329 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2122 4431 4677 669
Size: 988 281 184 2309 246 3079
Fragments arranged by size:
3079 2309 988 281 246 184
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7087
HpaI GTT'AAC
Cuts at: 4677 4677
Size: 7087
KasI G'GCGC_C
Cuts at: 3411 3470 3760 4048 3411
Size: 59 290 288 6450
Fragments arranged by size:
6450 290 288 59
KpnI G_GTAC'C
Cuts at: 2904 4412 2904
Size: 1508 5579
MfeI C'AATT_G
Cuts at: 4686 4686
Size: 7087
MluI A'CGCG_T
Cuts at: 6963 6963
Size: 7087
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3843 6201 6385 1305
Size: 2538 2358 184 2007
Fragments arranged by size:
2538 2358 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3311 4121 10
Size: 54 1425 343 1479 810 2976
Fragments arranged by size:
2976 1479 1425 810 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2391 3884 5045 5404 5563 518
Size: 648 1225 1493 1161 359 159 2042
Fragments arranged by size:
2042 1493 1225 1161 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2112 2173 2288 2489 2660 3507 4128 4278
Size: 506 61 115 201 171 847 621 150
Cuts at: 4278 4503 5180 6328 6573 1606
Size: 225 677 1148 245 2120
Fragments arranged by size:
2120 1148 847 677 621 506 245 225
201 171 150 115 61
NaeI GCC'GGC
Cuts at: 3987 3987
Size: 7087
NarI GG'CG_CC
Cuts at: 3412 3471 3761 4049 3412
Size: 59 290 288 6450
Fragments arranged by size:
6450 290 288 59
NcoI C'CATG_G
Cuts at: 513 1065 4116 513
Size: 552 3051 3484
Fragments arranged by size:
3484 3051 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7087
NgoMIV G'CCGG_C
Cuts at: 3985 3985
Size: 7087
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7087
NotI GC'GGCC_GC
Cuts at: 2292 4534 2292
Size: 2242 4845
NruI TCG'CGA
Cuts at: 4340 4340
Size: 7087
NspI r_CATG'y
Cuts at: 1132 1229 2071 3481 4445 5990 1132
Size: 97 842 1410 964 1545 2229
Fragments arranged by size:
2229 1545 1410 964 842 97
PasI CC'CwG_GG
Cuts at: 1348 2073 2833 2867 4246 1348
Size: 725 760 34 1379 4189
Fragments arranged by size:
4189 1379 760 725 34
PciI A'CATG_T
Cuts at: 5986 5986
Size: 7087
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2085 2833 1108
Size: 977 748 5362
Fragments arranged by size:
5362 977 748
PfoI T'CCnGG_A
Cuts at: 2030 2196 2030
Size: 166 6921
PmeI GTTT'AAAC
Cuts at: 4391 4391
Size: 7087
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5156 5188 6669 6701 5156
Size: 32 1481 32 5542
Fragments arranged by size:
5542 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2618 3809 4030 2618
Size: 1191 221 5675
Fragments arranged by size:
5675 1191 221
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7087
PsiI TTA'TAA
Cuts at: 4657 4657
Size: 7087
PspOMI G'GGCC_C
Cuts at: 2581 2846 2881 2581
Size: 265 35 6787
Fragments arranged by size:
6787 265 35
PstI C_TGCA'G
Cuts at: 830 4439 830
Size: 3609 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7087
PvuII CAG'CTG
Cuts at: 2173 3507 2173
Size: 1334 5753
RsrII CG'GwC_CG
Cuts at: 3857 3857
Size: 7087
SacII CC_GC'GG
Cuts at: 2113 2490 2113
Size: 377 6710
SalI G'TCGA_C
Cuts at: 1936 4429 1936
Size: 2493 4594
SanDI GG'GwC_CC
Cuts at: 2618 3809 2618
Size: 1191 5896
SbfI CC_TGCA'GG
Cuts at: 4439 4439
Size: 7087
ScaI AGT'ACT
Cuts at: 5335 7047 5335
Size: 1712 5375
SfcI C'TryA_G
Cuts at: 826 1045 4435 5570 6251 6442 826
Size: 219 3390 1135 681 191 1471
Fragments arranged by size:
3390 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2580 4250 2580
Size: 1670 5417
SfoI GGC'GCC
Cuts at: 3413 3472 3762 4050 3413
Size: 59 290 288 6450
Fragments arranged by size:
6450 290 288 59
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7087
SgrAI Cr'CCGG_yG
Cuts at: 1700 2527 4256 1700
Size: 827 1729 4531
Fragments arranged by size:
4531 1729 827
SmaI CCC'GGG
Cuts at: 1872 2215 2724 2907 3564 4074 4414 1872
Size: 343 509 183 657 510 340 4545
Fragments arranged by size:
4545 657 510 509 343 340 183
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5191 6092 6354 820
Size: 197 926 3248 901 262 1553
Fragments arranged by size:
3248 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4878 493
Size: 4385 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7087
SphI G_CATG'C
Cuts at: 1132 2071 4445 1132
Size: 939 2374 3774
Fragments arranged by size:
3774 2374 939
SspI AAT'ATT
Cuts at: 5 52 5011 5
Size: 47 4959 2081
Fragments arranged by size:
4959 2081 47
StuI AGG'CCT
Cuts at: 1517 3635 1517
Size: 2118 4969
StyI C'CwwG_G
Cuts at: 513 1065 3918 4116 4313 4526 513
Size: 552 2853 198 197 213 3074
Fragments arranged by size:
3074 2853 552 213 198 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2963 5114 5131 5284 5469 5914 325
Size: 1255 1383 2151 17 153 185 445 1498
Fragments arranged by size:
2151 1498 1383 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3288 5333 7045
Size: 274 80 33 51 1443 1311 2045 1712
Cuts at: 7045 96
Size: 138
Fragments arranged by size:
2045 1712 1443 1311 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7047 7079 7047
Size: 32 7055
Tth111I GACn'n_nGTC
Cuts at: 1316 3218 1316
Size: 1902 5185
XbaI T'CTAG_A
Cuts at: 4226 4423 4226
Size: 197 6890
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3232 3232
Size: 7087
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7087
XmaI C'CCGG_G
Cuts at: 1870 2213 2722 2905 3562 4072 4412 1870
Size: 343 509 183 657 510 340 4545
Fragments arranged by size:
4545 657 510 509 343 340 183
XmnI GAAnn'nnTTC
Cuts at: 3254 3341 5216 3254
Size: 87 1875 5125
Fragments arranged by size:
5125 1875 87
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4895 276
Size: 53 83 186 936 3361 2468
Fragments arranged by size:
3361 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII
SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NsiI
PacI PmlI PspXI PsrI SacI SapI SexAI SrfI
SwaI