Restriction map for
pFN21AE1276
(Circular) (Six-base) MAPSORT of: pFN21AE1276.seq Check: 6286 from: 1 to: 6961
pFN21AE1276 6961 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4771 278
Size: 53 83 186 936 3235 2468
Fragments arranged by size:
3235 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4304 1937
Size: 2367 4594
Acc65I G'GTAC_C
Cuts at: 3653 4282 3653
Size: 629 6332
AclI AA'CG_TT
Cuts at: 1563 5088 5461 1563
Size: 3525 373 3063
Fragments arranged by size:
3525 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2030 2111 2739 3954 4115 5024 6408 6676
Size: 398 81 628 1215 161 909 1384 268
Cuts at: 6676 1632
Size: 1917
Fragments arranged by size:
1917 1384 1215 909 628 398 268 161
81
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6764
AflIII A'CryG_T
Cuts at: 1203 2737 2922 3485 5860 6837 1203
Size: 1534 185 563 2375 977 1327
Fragments arranged by size:
2375 1534 1327 977 563 185
AgeI A'CCGG_T
Cuts at: 5792 5792
Size: 6961
AhdI GACnn_n'nnGTC
Cuts at: 3952 5690 3952
Size: 1738 5223
AleI CACnn'nnGTG
Cuts at: 1166 2958 1166
Size: 1792 5169
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6961
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3658 3690 3658
Size: 32 6929
AlwNI CAG_nnn'CTG
Cuts at: 4704 6276 4704
Size: 1572 5389
ApaLI G'TGCA_C
Cuts at: 5018 6174 5018
Size: 1156 5805
ApoI r'AATT_y
Cuts at: 1493 2545 2862 3007 3074 3195 4048 4270 4496
Size: 1052 317 145 67 121 853 222 226
Cuts at: 4496 4648 6757 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1052 853 317 226 222 152
145 121 67
AseI AT'TA_AT
Cuts at: 160 1032 3188 3983 5515 160
Size: 872 2156 795 1532 1606
Fragments arranged by size:
2156 1606 1532 872 795
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6961
AvaI C'yCGr_G
Cuts at: 1870 1943 4286 1870
Size: 73 2343 4545
Fragments arranged by size:
4545 2343 73
BamHI G'GATC_C
Cuts at: 4291 4658 4291
Size: 367 6594
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3653 4282 5737 618
Size: 325 217 627 1866 629 1455 1842
Fragments arranged by size:
1866 1842 1455 629 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4252 4280 1391
Size: 2861 28 4072
Fragments arranged by size:
4072 2861 28
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3942 4175 4182 928
Size: 971 2043 233 7 3707
Fragments arranged by size:
3707 2043 971 233 7
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5152 5186 1903
Size: 34 3215 34 3678
Fragments arranged by size:
3678 3215 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4853 6063 4853
Size: 1210 5751
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6961
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3035 5572 136
Size: 107 122 71 631 605 1363 2537 1525
Fragments arranged by size:
2537 1525 1363 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3641 6956 3641
Size: 3315 3646
BlpI GC'TnA_GC
Cuts at: 3000 4378 3000
Size: 1378 5583
Bme1580I G_kGCm'C
Cuts at: 1790 5022 6178 1790
Size: 3232 1156 2573
Fragments arranged by size:
3232 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6961
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3173 3659 4692
Size: 416 53 83 399 24 1756 486 1033
Cuts at: 4692 5650 442
Size: 958 1753
Fragments arranged by size:
1756 1753 1033 958 486 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6961
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5621 1239
Size: 4382 2579
Bpu10I CC'TnA_GC
Cuts at: 3699 3699
Size: 6961
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2837 5086 5951 6249 2837
Size: 2249 865 298 3549
Fragments arranged by size:
3549 2249 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5624 882
Size: 617 4125 2219
Fragments arranged by size:
4125 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4752 493
Size: 711 3548 2702
Fragments arranged by size:
3548 2702 711
BsaBI GATnn'nnATC
Cuts at: 2614 2784 2982 6955 2614
Size: 170 198 3973 2620
Fragments arranged by size:
3973 2620 198 170
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4768 5150 5785 275
Size: 53 83 186 936 3235 382 635 1451
Fragments arranged by size:
3235 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5394 5792 6066 6213 6624 5394
Size: 398 274 147 411 5731
Fragments arranged by size:
5731 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3658 3688 1178
Size: 30 165 30 2255 30 4451
Fragments arranged by size:
4451 2255 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2453 3561 1186
Size: 1267 1108 4586
Fragments arranged by size:
4586 1267 1108
BseYI C'CCAG_C
Cuts at: 3735 6164 6660 3735
Size: 2429 496 4036
Fragments arranged by size:
4036 2429 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2661 1163
Size: 573 925 5463
Fragments arranged by size:
5463 925 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4411 5172 6200 1540
Size: 275 186 2410 761 1028 2301
Fragments arranged by size:
2410 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2955 4252 5022 5107 6178 2955
Size: 1297 770 85 1071 3738
Fragments arranged by size:
3738 1297 1071 770 85
BsmI GAATG_Cn'
Cuts at: 3227 3691 4472 4565 3227
Size: 464 781 93 5623
Fragments arranged by size:
5623 781 464 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2955 4252 4280 5022 5107 6178 1391
Size: 399 1165 1297 28 742 85 1071 2174
Fragments arranged by size:
2174 1297 1165 1071 742 399 85 28
BspEI T'CCGG_A
Cuts at: 6624 6624
Size: 6961
BspHI T'CATG_A
Cuts at: 2059 4850 6580 2059
Size: 2791 1730 2440
Fragments arranged by size:
2791 2440 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3832 4316 844
Size: 2988 484 3489
Fragments arranged by size:
3489 2988 484
BsrBI CCG'CTC
Cuts at: 1394 1823 4848 1394
Size: 429 3025 3507
Fragments arranged by size:
3507 3025 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5456 5630 65
Size: 1183 4208 174 1396
Fragments arranged by size:
4208 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5605 5792 1700
Size: 110 3795 187 2869
Fragments arranged by size:
3795 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6961
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6961
BssSI C'ACGA_G
Cuts at: 5021 6033 5021
Size: 1012 5949
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2495 794
Size: 1701 5260
BstBI TT'CG_AA
Cuts at: 5765 5765
Size: 6961
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6961
BstXI CCAn_nnnn'nTGG
Cuts at: 2331 2331
Size: 6961
BstYI r'GATC_y
Cuts at: 1972 2691 2785 3641 4291 4658 5043 5060 6512
Size: 719 94 856 650 367 385 17 1452
Cuts at: 6512 6598 6610 6956 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 856 719 650 385 367 346
94 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2409 2409
Size: 6961
BtgI C'CryG_G
Cuts at: 513 1065 2004 3030 3216 513
Size: 552 939 1026 186 4258
Fragments arranged by size:
4258 1026 939 552 186
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6222
Fragments arranged by size:
6222 683 56
BtsI GCAGTG_nn'
Cuts at: 2029 2381 4477 5271 5291 2029
Size: 352 2096 794 20 3699
Fragments arranged by size:
3699 2096 794 352 20
ClaI AT'CG_AT
Cuts at: 1554 2230 1554
Size: 676 6285
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6926
DraI TTT'AAA
Cuts at: 2247 2355 3777 4265 2247
Size: 108 1422 488 4943
Fragments arranged by size:
4943 1422 488 108
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5968 809
Size: 507 4652 1802
Fragments arranged by size:
4652 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3027 4408 5297
Size: 54 1425 50 275 18 1197 1381 889
Cuts at: 5297 6926 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1381 1197 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4408 1537
Size: 275 2596 4090
Fragments arranged by size:
4090 2596 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2006 2430 3347 3579 4891 1353
Size: 653 424 917 232 1312 3423
Fragments arranged by size:
3423 1312 917 653 424 232
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5548 5922 6068 387
Size: 1064 138 3959 374 146 1280
Fragments arranged by size:
3959 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4250 4250
Size: 6961
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2030 2111 2739 3954 4115 5024 5621
Size: 393 398 81 628 1215 161 909 597
Cuts at: 5621 6408 6676 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1215 909 787 628 597 398 393
268 161 81
EcoNI CCTnn'n_nnAGG
Cuts at: 3809 3809
Size: 6961
EcoO109I rG'GnC_Cy
Cuts at: 2267 2267
Size: 6961
EcoRI G'AATT_C
Cuts at: 2545 2862 3007 3195 4270 4648 2545
Size: 317 145 188 1075 378 4858
Fragments arranged by size:
4858 1075 378 317 188 145
EcoRV GAT'ATC
Cuts at: 4211 4211
Size: 6961
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2352 4199 2352
Size: 1847 5114
FspI TGC'GCA
Cuts at: 5467 5467
Size: 6961
HaeII r_GCGC'y
Cuts at: 1678 3431 6108 1678
Size: 1753 2677 2531
Fragments arranged by size:
2677 2531 1753
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2251 2283 2909 2941 3938
Size: 32 135 32 679 32 626 32 997
Cuts at: 3938 3970 5602 5634 5676 5708 1373
Size: 32 1632 32 42 32 2626
Fragments arranged by size:
2626 1632 997 679 626 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3930 4305 4551 669
Size: 988 281 1992 375 246 3079
Fragments arranged by size:
3079 1992 988 375 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6961
HpaI GTT'AAC
Cuts at: 4551 4551
Size: 6961
KpnI G_GTAC'C
Cuts at: 3657 4286 3657
Size: 629 6332
MfeI C'AATT_G
Cuts at: 2725 4560 2725
Size: 1835 5126
MluI A'CGCG_T
Cuts at: 6837 6837
Size: 6961
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6075 6259 1305
Size: 4770 184 2007
Fragments arranged by size:
4770 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5139
Fragments arranged by size:
5139 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2064 2958 4919 5278 5437 518
Size: 648 898 894 1961 359 159 2042
Fragments arranged by size:
2042 1961 898 894 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2837 4377 5054 6202 6447 1606
Size: 1231 1540 677 1148 245 2120
Fragments arranged by size:
2120 1540 1231 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3216 513
Size: 552 939 1212 4258
Fragments arranged by size:
4258 1212 939 552
NdeI CA'TA_TG
Cuts at: 387 2655 387
Size: 2268 4693
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6961
NotI GC'GGCC_GC
Cuts at: 4408 4408
Size: 6961
NspI r_CATG'y
Cuts at: 1132 1229 2741 2926 3489 3952 4319 5864 1132
Size: 97 1512 185 563 463 367 1545 2229
Fragments arranged by size:
2229 1545 1512 563 463 367 185 97
PasI CC'CwG_GG
Cuts at: 1348 2696 1348
Size: 1348 5613
PciI A'CATG_T
Cuts at: 2737 2922 3485 5860 2737
Size: 185 563 2375 3838
Fragments arranged by size:
3838 2375 563 185
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6961
PfoI T'CCnGG_A
Cuts at: 2426 2509 2426
Size: 83 6878
PmeI GTTT'AAAC
Cuts at: 4265 4265
Size: 6961
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3658 3690 5030 5062 6543 6575 3658
Size: 32 1340 32 1481 32 4044
Fragments arranged by size:
4044 1481 1340 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2267 2267
Size: 6961
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6961
PsiI TTA'TAA
Cuts at: 4531 4531
Size: 6961
PstI C_TGCA'G
Cuts at: 830 4313 830
Size: 3483 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6961
PvuII CAG'CTG
Cuts at: 2837 2837
Size: 6961
SacI G_AGCT'C
Cuts at: 4252 4252
Size: 6961
SalI G'TCGA_C
Cuts at: 1936 4303 1936
Size: 2367 4594
SbfI CC_TGCA'GG
Cuts at: 4313 4313
Size: 6961
ScaI AGT'ACT
Cuts at: 4219 4240 5209 6921 4219
Size: 21 969 1712 4259
Fragments arranged by size:
4259 1712 969 21
SfcI C'TryA_G
Cuts at: 826 1045 3111 4309 5444 6125 6316 826
Size: 219 2066 1198 1135 681 191 1471
Fragments arranged by size:
2066 1471 1198 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6961
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6961
SmaI CCC'GGG
Cuts at: 1872 4288 1872
Size: 2416 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2816 5065 5966 6228 820
Size: 197 926 873 2249 901 262 1553
Fragments arranged by size:
2249 1553 926 901 873 262 197
SnaBI TAC'GTA
Cuts at: 493 4752 493
Size: 4259 2702
SpeI A'CTAG_T
Cuts at: 152 2957 3883 152
Size: 2805 926 3230
Fragments arranged by size:
3230 2805 926
SphI G_CATG'C
Cuts at: 1132 4319 1132
Size: 3187 3774
SspI AAT'ATT
Cuts at: 5 52 4885 5
Size: 47 4833 2081
Fragments arranged by size:
4833 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6961
StyI C'CwwG_G
Cuts at: 513 1065 2004 2270 3216 4400 513
Size: 552 939 266 946 1184 3074
Fragments arranged by size:
3074 1184 946 939 552 266
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3028 4988 5005 5158 5343 5788 325
Size: 1255 1448 1960 17 153 185 445 1498
Fragments arranged by size:
1960 1498 1448 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3244 4217 4238
Size: 274 80 33 51 1443 1267 973 21
Cuts at: 4238 5207 6919 96
Size: 969 1712 138
Fragments arranged by size:
1712 1443 1267 973 969 274 138 80
51 33 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3324 3356 6921 6953 3324
Size: 32 3565 32 3332
Fragments arranged by size:
3565 3332 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6961
XbaI T'CTAG_A
Cuts at: 3088 4297 3088
Size: 1209 5752
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3022 3022
Size: 6961
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6961
XmaI C'CCGG_G
Cuts at: 1870 4286 1870
Size: 2416 4545
XmnI GAAnn'nnTTC
Cuts at: 2307 2739 2884 3291 3762 5090 2307
Size: 432 145 407 471 1328 4178
Fragments arranged by size:
4178 1328 471 432 407 145
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4769 276
Size: 53 83 186 936 3235 2468
Fragments arranged by size:
3235 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I DraIII FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI