Restriction map for
pFN21AE1274
(Circular) (Six-base) MAPSORT of: pFN21AE1274.seq Check: 7415 from: 1 to: 6838
pFN21AE1274 6838 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4648 278
Size: 53 83 186 936 3112 2468
Fragments arranged by size:
3112 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2642 4181 1937
Size: 705 1539 4594
Fragments arranged by size:
4594 1539 705
Acc65I G'GTAC_C
Cuts at: 4159 4159
Size: 6838
AclI AA'CG_TT
Cuts at: 1563 4965 5338 1563
Size: 3402 373 3063
Fragments arranged by size:
3402 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3144 3249 3677 3770 4109 4901 6285 6553
Size: 1512 105 428 93 339 792 1384 268
Cuts at: 6553 1632
Size: 1917
Fragments arranged by size:
1917 1512 1384 792 428 339 268 105
93
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6641
AflIII A'CryG_T
Cuts at: 1203 2199 2376 3542 5737 6714 1203
Size: 996 177 1166 2195 977 1327
Fragments arranged by size:
2195 1327 1166 996 977 177
AgeI A'CCGG_T
Cuts at: 5669 5669
Size: 6838
AhdI GACnn_n'nnGTC
Cuts at: 5567 5567
Size: 6838
AleI CACnn'nnGTG
Cuts at: 1166 3230 1166
Size: 2064 4774
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6838
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2012 2044 2012
Size: 32 6806
AlwNI CAG_nnn'CTG
Cuts at: 4098 4132 4581 6153 4098
Size: 34 449 1572 4783
Fragments arranged by size:
4783 1572 449 34
ApaLI G'TGCA_C
Cuts at: 4895 6051 4895
Size: 1156 5682
ApoI r'AATT_y
Cuts at: 1493 2209 2615 3287 3302 3635 3837 3853 3868
Size: 716 406 672 15 333 202 16 15
Cuts at: 3868 3908 3942 4147 4373 4525 6634 1493
Size: 40 34 205 226 152 2109 1697
Fragments arranged by size:
2109 1697 716 672 406 333 226 205
202 152 40 34 16 15 15
AseI AT'TA_AT
Cuts at: 160 1032 4104 5392 160
Size: 872 3072 1288 1606
Fragments arranged by size:
3072 1606 1288 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6838
AvaI C'yCGr_G
Cuts at: 1870 1943 2046 3022 3999 4163 1870
Size: 73 103 976 977 164 4545
Fragments arranged by size:
4545 977 976 164 103 73
BamHI G'GATC_C
Cuts at: 3437 4168 4535 3437
Size: 731 367 5740
Fragments arranged by size:
5740 731 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2056 2669 4159 5614 618
Size: 325 217 627 269 613 1490 1455 1842
Fragments arranged by size:
1842 1490 1455 627 613 325 269 217
BanII G_rGCy'C
Cuts at: 1391 2047 4157 1391
Size: 656 2110 4072
Fragments arranged by size:
4072 2110 656
BbeI G_GCGC'C
Cuts at: 2673 2673
Size: 6838
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2842 2947 3168 3335 928
Size: 971 943 105 221 167 4431
Fragments arranged by size:
4431 971 943 221 167 105
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2141 2175 5029 5063 1903
Size: 34 204 34 2854 34 3678
Fragments arranged by size:
3678 2854 204 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2671 3328 4730 5940 2671
Size: 657 1402 1210 3569
Fragments arranged by size:
3569 1402 1210 657
BclI T'GATC_A
Cuts at: 1548 2091 3978 1548
Size: 543 1887 4408
Fragments arranged by size:
4408 1887 543
BfrBI ATG'CAT
Cuts at: 2969 2969
Size: 6838
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5449 136
Size: 107 122 71 631 605 3777 1525
Fragments arranged by size:
3777 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4113 6833 4113
Size: 2720 4118
BlpI GC'TnA_GC
Cuts at: 4255 4255
Size: 6838
Bme1580I G_kGCm'C
Cuts at: 1790 4899 6055 1790
Size: 3109 1156 2573
Fragments arranged by size:
3109 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3510 1318
Size: 2192 4646
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4569 5527 442
Size: 416 53 83 399 24 3152 958 1753
Fragments arranged by size:
3152 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6838
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3634 3634
Size: 6838
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2484 3416 3497 3923 5498 1239
Size: 1245 932 81 426 1575 2579
Fragments arranged by size:
2579 1575 1245 932 426 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3940 4963 5828 6126 3940
Size: 1023 865 298 4652
Fragments arranged by size:
4652 1023 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4060 5501 882
Size: 617 2561 1441 2219
Fragments arranged by size:
2561 2219 1441 617
BsaAI yAC'GTr
Cuts at: 493 1204 4629 493
Size: 711 3425 2702
Fragments arranged by size:
3425 2702 711
BsaBI GATnn'nnATC
Cuts at: 6832 6832
Size: 6838
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2670 4645 5027 5662
Size: 53 83 186 936 1137 1975 382 635
Cuts at: 5662 275
Size: 1451
Fragments arranged by size:
1975 1451 1137 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5271 5669 5943 6090 6501 5271
Size: 398 274 147 411 5608
Fragments arranged by size:
5608 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2765 2795 1178
Size: 30 165 30 1362 30 5221
Fragments arranged by size:
5221 1362 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6838
BseYI C'CCAG_C
Cuts at: 3264 6041 6537 3264
Size: 2777 496 3565
Fragments arranged by size:
3565 2777 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2057 2456 4073 1163
Size: 573 321 399 1617 3928
Fragments arranged by size:
3928 1617 573 399 321
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4288 5049 6077 1540
Size: 275 186 2287 761 1028 2301
Fragments arranged by size:
2301 2287 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4899 4984 6055 4899
Size: 85 1071 5682
Fragments arranged by size:
5682 1071 85
BsmI GAATG_Cn'
Cuts at: 2164 2969 2971 3978 4349 4442 2164
Size: 805 2 1007 371 93 4560
Fragments arranged by size:
4560 1007 805 371 93 2
BsmBI CGTCTCn'nnnn_
Cuts at: 3744 3744
Size: 6838
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2047 4157 4899 4984 6055 1391
Size: 399 257 2110 742 85 1071 2174
Fragments arranged by size:
2174 2110 1071 742 399 257 85
BspEI T'CCGG_A
Cuts at: 6501 6501
Size: 6838
BspHI T'CATG_A
Cuts at: 4727 6457 4727
Size: 1730 5108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2584 4193 844
Size: 1740 1609 3489
Fragments arranged by size:
3489 1740 1609
BsrBI CCG'CTC
Cuts at: 1394 1823 2155 2167 4725 1394
Size: 429 332 12 2558 3507
Fragments arranged by size:
3507 2558 429 332 12
BsrDI GCAATG_nn'
Cuts at: 65 1248 5333 5507 65
Size: 1183 4085 174 1396
Fragments arranged by size:
4085 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2224 5482 5669 1700
Size: 110 414 3258 187 2869
Fragments arranged by size:
3258 2869 414 187 110
BsrGI T'GTAC_A
Cuts at: 96 2142 96
Size: 2046 4792
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6838
BssSI C'ACGA_G
Cuts at: 4898 5910 4898
Size: 1012 5826
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6838
BstBI TT'CG_AA
Cuts at: 5642 5642
Size: 6838
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6838
BstXI CCAn_nnnn'nTGG
Cuts at: 2950 2950
Size: 6838
BstYI r'GATC_y
Cuts at: 1972 2872 3437 3652 4113 4168 4535 4920 4937
Size: 900 565 215 461 55 367 385 17
Cuts at: 4937 6389 6475 6487 6833 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 900 565 461 385 367 346
215 86 55 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3800 4041 513
Size: 552 2735 241 3310
Fragments arranged by size:
3310 2735 552 241
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6099
Fragments arranged by size:
6099 683 56
BtsI GCAGTG_nn'
Cuts at: 4354 5148 5168 4354
Size: 794 20 6024
Fragments arranged by size:
6024 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6838
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2051 2086 529
Size: 35 1487 35 5281
Fragments arranged by size:
5281 1487 35 35
DraI TTT'AAA
Cuts at: 3247 4142 3247
Size: 895 5943
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5845 809
Size: 507 4529 1802
Fragments arranged by size:
4529 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3252 3797 4038
Size: 54 1425 50 275 18 1422 545 241
Cuts at: 4038 4285 5174 6803 8
Size: 247 889 1629 43
Fragments arranged by size:
1629 1425 1422 889 545 275 247 241
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4285 1537
Size: 275 2473 4090
Fragments arranged by size:
4090 2473 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2325 3008 3124 4768 1353
Size: 972 683 116 1644 3423
Fragments arranged by size:
3423 1644 972 683 116
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5425 5799 5945 387
Size: 1064 138 3836 374 146 1280
Fragments arranged by size:
3836 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2484 3144 3249 3416 3497 3677 3770
Size: 393 852 660 105 167 81 180 93
Cuts at: 3770 3923 4109 4901 5498 6285 6553 1239
Size: 153 186 792 597 787 268 1524
Fragments arranged by size:
1524 852 792 787 660 597 393 268
186 180 167 153 105 93 81
EcoNI CCTnn'n_nnAGG
Cuts at: 2456 2456
Size: 6838
EcoO109I rG'GnC_Cy
Cuts at: 3011 3471 3011
Size: 460 6378
EcoRI G'AATT_C
Cuts at: 3287 4147 4525 3287
Size: 860 378 5600
Fragments arranged by size:
5600 860 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3230 4045 3230
Size: 815 6023
FspI TGC'GCA
Cuts at: 5344 5344
Size: 6838
HaeII r_GCGC'y
Cuts at: 1678 2419 2673 3494 4051 5985 1678
Size: 741 254 821 557 1934 2531
Fragments arranged by size:
2531 1934 821 741 557 254
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2678 2710 3364 3396 5479
Size: 32 135 32 1106 32 654 32 2083
Cuts at: 5479 5511 5553 5585 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2083 1106 654 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2983 4182 4428 669
Size: 988 281 1045 1199 246 3079
Fragments arranged by size:
3079 1199 1045 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3241 3725 748
Size: 2493 484 3861
Fragments arranged by size:
3861 2493 484
HpaI GTT'AAC
Cuts at: 4428 4428
Size: 6838
KasI G'GCGC_C
Cuts at: 2669 2669
Size: 6838
KpnI G_GTAC'C
Cuts at: 4163 4163
Size: 6838
MfeI C'AATT_G
Cuts at: 4437 4437
Size: 6838
MluI A'CGCG_T
Cuts at: 6714 6714
Size: 6838
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2782 5952 6136 1305
Size: 1477 3170 184 2007
Fragments arranged by size:
3170 2007 1477 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3254 3799 4040 10
Size: 54 1425 343 1422 545 241 2808
Fragments arranged by size:
2808 1425 1422 545 343 241 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2204 3230 3368 4796 5155 5314 518
Size: 648 1038 1026 138 1428 359 159 2042
Fragments arranged by size:
2042 1428 1038 1026 648 359 159 138
MspA1I CmG'CkG
Cuts at: 1606 2612 3502 4062 4254 4931 6079 6324 1606
Size: 1006 890 560 192 677 1148 245 2120
Fragments arranged by size:
2120 1148 1006 890 677 560 245 192
NarI GG'CG_CC
Cuts at: 2670 2670
Size: 6838
NcoI C'CATG_G
Cuts at: 513 1065 4041 513
Size: 552 2976 3310
Fragments arranged by size:
3310 2976 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6838
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6838
NotI GC'GGCC_GC
Cuts at: 4285 4285
Size: 6838
NsiI A_TGCA'T
Cuts at: 2971 2971
Size: 6838
NspI r_CATG'y
Cuts at: 1132 1229 2203 2915 3546 3773 4196 5741 1132
Size: 97 974 712 631 227 423 1545 2229
Fragments arranged by size:
2229 1545 974 712 631 423 227 97
PasI CC'CwG_GG
Cuts at: 1348 2014 1348
Size: 666 6172
PciI A'CATG_T
Cuts at: 2199 3542 5737 2199
Size: 1343 2195 3300
Fragments arranged by size:
3300 2195 1343
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2085 2227 2761 1108
Size: 977 142 534 5185
Fragments arranged by size:
5185 977 534 142
PmeI GTTT'AAAC
Cuts at: 4142 4142
Size: 6838
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4907 4939 6420 6452 4907
Size: 32 1481 32 5293
Fragments arranged by size:
5293 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3011 3471 3011
Size: 460 6378
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6838
PsiI TTA'TAA
Cuts at: 4408 4408
Size: 6838
PspXI vC'TCGA_Gb
Cuts at: 3999 3999
Size: 6838
PstI C_TGCA'G
Cuts at: 830 2882 4190 830
Size: 2052 1308 3478
Fragments arranged by size:
3478 2052 1308
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6838
PvuII CAG'CTG
Cuts at: 2612 3502 2612
Size: 890 5948
RsrII CG'GwC_CG
Cuts at: 2169 2169
Size: 6838
SalI G'TCGA_C
Cuts at: 1936 4180 1936
Size: 2244 4594
SanDI GG'GwC_CC
Cuts at: 3471 3471
Size: 6838
SbfI CC_TGCA'GG
Cuts at: 4190 4190
Size: 6838
ScaI AGT'ACT
Cuts at: 2597 5086 6798 2597
Size: 2489 1712 2637
Fragments arranged by size:
2637 2489 1712
SfcI C'TryA_G
Cuts at: 826 1045 2878 4186 5321 6002 6193 826
Size: 219 1833 1308 1135 681 191 1471
Fragments arranged by size:
1833 1471 1308 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2671 2671
Size: 6838
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6838
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6838
SmaI CCC'GGG
Cuts at: 1872 2048 4165 1872
Size: 176 2117 4545
Fragments arranged by size:
4545 2117 176
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3919 3999 4942 5843 6105 820
Size: 197 926 1976 80 943 901 262 1553
Fragments arranged by size:
1976 1553 943 926 901 262 197 80
SnaBI TAC'GTA
Cuts at: 493 4629 493
Size: 4136 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6838
SphI G_CATG'C
Cuts at: 1132 4196 1132
Size: 3064 3774
SspI AAT'ATT
Cuts at: 5 52 3892 4762 5
Size: 47 3840 870 2081
Fragments arranged by size:
3840 2081 870 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6838
StyI C'CwwG_G
Cuts at: 513 1065 2079 2452 2768 3007 4041 4277 513
Size: 552 1014 373 316 239 1034 236 3074
Fragments arranged by size:
3074 1034 1014 552 373 316 239 236
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4865 4882 5035 5220 5665 325
Size: 1255 3285 17 153 185 445 1498
Fragments arranged by size:
3285 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2142 2595 2721
Size: 274 80 33 51 1443 165 453 126
Cuts at: 2721 3531 5084 6796 96
Size: 810 1553 1712 138
Fragments arranged by size:
1712 1553 1443 810 453 274 165 138
126 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6798 6830 6798
Size: 32 6806
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6838
XbaI T'CTAG_A
Cuts at: 4174 4174
Size: 6838
XhoI C'TCGA_G
Cuts at: 1943 3999 1943
Size: 2056 4782
XmaI C'CCGG_G
Cuts at: 1870 2046 4163 1870
Size: 176 2117 4545
Fragments arranged by size:
4545 2117 176
XmnI GAAnn'nnTTC
Cuts at: 2319 2969 3646 3815 3872 4967 2319
Size: 650 677 169 57 1095 4190
Fragments arranged by size:
4190 1095 677 650 169 57
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4646 276
Size: 53 83 186 936 3112 2468
Fragments arranged by size:
3112 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI PvuII RsrII SalI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI Bpu10I
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI
NaeI NgoMIV NruI PacI PfoI PmlI PspOMI PsrI
SacI SacII SapI SexAI SfiI SrfI SwaI XcmI